Restriction map for
pFN21AA1803
(Linear) (Six-base) MAPSORT of: KIAA1803.seq Check: 7120 from: 1 to: 4236
KIAA1803.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3649 4041 4236
Size: 3649 392 195
Fragments arranged by size:
3649 392 195
AccI GT'mk_AC
Cuts at: 0 831 1076 4236
Size: 831 245 3160
Fragments arranged by size:
3160 831 245
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 896 1430 3031 3143 3299 3917 4236
Size: 896 534 1601 112 156 618 319
Fragments arranged by size:
1601 896 618 534 319 156 112
AflIII A'CryG_T
Cuts at: 0 4011 4236
Size: 4011 225
AgeI A'CCGG_T
Cuts at: 0 1265 4236
Size: 1265 2971
AleI CACnn'nnGTG
Cuts at: 0 1411 4236
Size: 1411 2825
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2360 4236
Size: 2360 1876
AlwNI CAG_nnn'CTG
Cuts at: 0 653 811 2539 2733 4236
Size: 653 158 1728 194 1503
Fragments arranged by size:
1728 1503 653 194 158
ApaI G_GGCC'C
Cuts at: 0 23 3469 4236
Size: 23 3446 767
Fragments arranged by size:
3446 767 23
ApaLI G'TGCA_C
Cuts at: 0 948 1180 2461 4236
Size: 948 232 1281 1775
Fragments arranged by size:
1775 1281 948 232
ApoI r'AATT_y
Cuts at: 0 1345 2284 2446 3298 3307 3967 4236
Size: 1345 939 162 852 9 660 269
Fragments arranged by size:
1345 939 852 660 269 162 9
AvaI C'yCGr_G
Cuts at: 0 1390 1396 3457 4236
Size: 1390 6 2061 779
Fragments arranged by size:
2061 1390 779 6
BanI G'GyrC_C
Cuts at: 0 1069 1258 1517 2403 2611 4236
Size: 1069 189 259 886 208 1625
Fragments arranged by size:
1625 1069 886 259 208 189
BanII G_rGCy'C
Cuts at: 0 23 506 1397 1420 1463 1599 3164 3469
Size: 23 483 891 23 43 136 1565 305
Cuts at: 3469 3850 3997 4236
Size: 381 147 239
Fragments arranged by size:
1565 891 483 381 305 239 147 136
43 23 23
BbeI G_GCGC'C
Cuts at: 0 1262 2615 4236
Size: 1262 1353 1621
Fragments arranged by size:
1621 1353 1262
BbsI GAAGACnn'nnnn_
Cuts at: 0 3140 3905 4236
Size: 3140 765 331
Fragments arranged by size:
3140 765 331
BbvCI CC'TCA_GC
Cuts at: 0 1169 1796 4236
Size: 1169 627 2440
Fragments arranged by size:
2440 1169 627
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 224 258 1684 1718 2465 2499 4236
Size: 224 34 1426 34 747 34 1737
Fragments arranged by size:
1737 1426 747 224 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2680 4236
Size: 2680 1556
BclI T'GATC_A
Cuts at: 0 1763 4236
Size: 1763 2473
BfrBI ATG'CAT
Cuts at: 0 2127 4236
Size: 2127 2109
BglII A'GATC_T
Cuts at: 0 3474 4236
Size: 3474 762
BlpI GC'TnA_GC
Cuts at: 0 1801 4236
Size: 1801 2435
Bme1580I G_kGCm'C
Cuts at: 0 23 952 1184 1635 2465 3446 3469 4236
Size: 23 929 232 451 830 981 23 767
Fragments arranged by size:
981 929 830 767 451 232 23 23
BmgBI CAC'GTC
Cuts at: 0 422 1221 2505 4236
Size: 422 799 1284 1731
Fragments arranged by size:
1731 1284 799 422
BmrI ACTGGGnnnn_n'
Cuts at: 0 1356 4236
Size: 1356 2880
BmtI G_CTAG'C
Cuts at: 0 910 4236
Size: 910 3326
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2274 4236
Size: 2274 1962
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 1716 3630 3646 3834 4231 4236
Size: 319 1397 1914 16 188 397 5
Fragments arranged by size:
1914 1397 397 319 188 16 5
Bpu10I CC'TnA_GC
Cuts at: 0 1169 1796 2048 4236
Size: 1169 627 252 2188
Fragments arranged by size:
2188 1169 627 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3010 4236
Size: 3010 1226
BsaI GGTCTCn'nnnn_
Cuts at: 0 1503 3718 4236
Size: 1503 2215 518
Fragments arranged by size:
2215 1503 518
BsaAI yAC'GTr
Cuts at: 0 3432 4236
Size: 3432 804
BsaHI Gr'CG_yC
Cuts at: 0 1259 2603 2612 3339 4236
Size: 1259 1344 9 727 897
Fragments arranged by size:
1344 1259 897 727 9
BsaWI w'CCGG_w
Cuts at: 0 140 1265 1514 4236
Size: 140 1125 249 2722
Fragments arranged by size:
2722 1125 249 140
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1450 1480 1483 1513 1525 1555 3085 3115
Size: 1450 30 3 30 12 30 1530 30
Cuts at: 3115 4236
Size: 1121
Fragments arranged by size:
1530 1450 1121 30 30 30 30 12
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1240 1456 1459 1471 2020 2328 2763 3067
Size: 1240 216 3 12 549 308 435 304
Cuts at: 3067 3072 3090 3303 3475 4236
Size: 5 18 213 172 761
Fragments arranged by size:
1240 761 549 435 308 304 216 213
172 18 12 5 3
BseYI C'CCAG_C
Cuts at: 0 586 1598 2523 3439 4236
Size: 586 1012 925 916 797
Fragments arranged by size:
1012 925 916 797 586
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3526 4236
Size: 3526 710
BsiEI CG_ry'CG
Cuts at: 0 249 1961 2680 4236
Size: 249 1712 719 1556
Fragments arranged by size:
1712 1556 719 249
BsiHKAI G_wGCw'C
Cuts at: 0 327 346 952 1184 1843 1852 2465 2983
Size: 327 19 606 232 659 9 613 518
Cuts at: 2983 3850 4236
Size: 867 386
Fragments arranged by size:
867 659 613 606 518 386 327 232
19 9
BsmI GAATG_Cn'
Cuts at: 0 703 4221 4236
Size: 703 3518 15
Fragments arranged by size:
3518 703 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1222 4236
Size: 1222 3014
Bsp1286I G_dGCh'C
Cuts at: 0 23 327 346 506 952 1184 1397 1420
Size: 23 304 19 160 446 232 213 23
Cuts at: 1420 1463 1599 1635 1843 1852 2465 2983 3164
Size: 43 136 36 208 9 613 518 181
Cuts at: 3164 3446 3469 3850 3997 4236
Size: 282 23 381 147 239
Fragments arranged by size:
613 518 446 381 304 282 239 232
213 208 181 160 147 136 43 36
23 23 23 19 9
BspHI T'CATG_A
Cuts at: 0 2742 4236
Size: 2742 1494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3649 4041 4236
Size: 3649 392 195
Fragments arranged by size:
3649 392 195
BsrBI CCG'CTC
Cuts at: 0 303 4236
Size: 303 3933
BsrDI GCAATG_nn'
Cuts at: 0 1089 2523 2691 4236
Size: 1089 1434 168 1545
Fragments arranged by size:
1545 1434 1089 168
BsrFI r'CCGG_y
Cuts at: 0 1265 2918 3578 4236
Size: 1265 1653 660 658
Fragments arranged by size:
1653 1265 660 658
BssSI C'ACGA_G
Cuts at: 0 1545 4236
Size: 1545 2691
BstAPI GCAn_nnn'nTGC
Cuts at: 0 956 1091 2084 2353 2733 2891 3389 4236
Size: 956 135 993 269 380 158 498 847
Fragments arranged by size:
993 956 847 498 380 269 158 135
BstBI TT'CG_AA
Cuts at: 0 234 662 4236
Size: 234 428 3574
Fragments arranged by size:
3574 428 234
BstEII G'GTnAC_C
Cuts at: 0 1161 4236
Size: 1161 3075
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1941 1995 4236
Size: 1941 54 2241
Fragments arranged by size:
2241 1941 54
BstYI r'GATC_y
Cuts at: 0 1238 3474 4236
Size: 1238 2236 762
Fragments arranged by size:
2236 1238 762
BstZ17I GTA'TAC
Cuts at: 0 832 4236
Size: 832 3404
BtgI C'CryG_G
Cuts at: 0 1116 2859 3033 3061 3927 4236
Size: 1116 1743 174 28 866 309
Fragments arranged by size:
1743 1116 866 309 174 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 447 3872 4236
Size: 447 3425 364
Fragments arranged by size:
3425 447 364
BtsI GCAGTG_nn'
Cuts at: 0 346 471 1716 1829 2105 2640 3291 4236
Size: 346 125 1245 113 276 535 651 945
Fragments arranged by size:
1245 945 651 535 346 276 125 113
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3342 3377 4236
Size: 3342 35 859
Fragments arranged by size:
3342 859 35
DraI TTT'AAA
Cuts at: 0 3411 4236
Size: 3411 825
DraIII CAC_nnn'GTG
Cuts at: 0 1509 3537 4236
Size: 1509 2028 699
Fragments arranged by size:
2028 1509 699
EaeI y'GGCC_r
Cuts at: 0 37 224 1958 2677 2765 4236
Size: 37 187 1734 719 88 1471
Fragments arranged by size:
1734 1471 719 187 88 37
EagI C'GGCC_G
Cuts at: 0 1958 2677 4236
Size: 1958 719 1559
Fragments arranged by size:
1958 1559 719
EarI CTCTTCn'nnn_
Cuts at: 0 791 1627 1997 2393 3545 4236
Size: 791 836 370 396 1152 691
Fragments arranged by size:
1152 836 791 691 396 370
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2569 4236
Size: 2569 1667
EcoICRI GAG'CTC
Cuts at: 0 3848 4236
Size: 3848 388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 896 1430 1716 3031 3143 3299 3630
Size: 319 577 534 286 1315 112 156 331
Cuts at: 3630 3646 3834 3917 4231 4236
Size: 16 188 83 314 5
Fragments arranged by size:
1315 577 534 331 319 314 286 188
156 112 83 16 5
EcoO109I rG'GnC_Cy
Cuts at: 0 19 20 808 2151 3465 3466 4236
Size: 19 1 788 1343 1314 1 770
Fragments arranged by size:
1343 1314 788 770 19 1 1
EcoRI G'AATT_C
Cuts at: 0 2284 3298 3307 4236
Size: 2284 1014 9 929
Fragments arranged by size:
2284 1014 929 9
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1860 2115 2181 3304 3651 4236
Size: 1860 255 66 1123 347 585
Fragments arranged by size:
1860 1123 585 347 255 66
HaeII r_GCGC'y
Cuts at: 0 1262 1692 2615 4236
Size: 1262 430 923 1621
Fragments arranged by size:
1621 1262 923 430
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1450 1482 2873 2905 3057 3089 4052 4084
Size: 1450 32 1391 32 152 32 963 32
Cuts at: 4084 4236
Size: 152
Fragments arranged by size:
1450 1391 963 152 152 32 32 32
32
HincII GTy'rAC
Cuts at: 0 3807 4236
Size: 3807 429
HindIII A'AGCT_T
Cuts at: 0 3370 4236
Size: 3370 866
HpaI GTT'AAC
Cuts at: 0 3807 4236
Size: 3807 429
KasI G'GCGC_C
Cuts at: 0 1258 2611 4236
Size: 1258 1353 1625
Fragments arranged by size:
1625 1353 1258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2642 3777 4236
Size: 2642 1135 459
Fragments arranged by size:
2642 1135 459
MscI TGG'CCA
Cuts at: 0 2767 4236
Size: 2767 1469
MslI CAynn'nnrTG
Cuts at: 0 330 565 945 1024 1159 1411 1586 1993
Size: 330 235 380 79 135 252 175 407
Cuts at: 1993 2880 2951 4236
Size: 887 71 1285
Fragments arranged by size:
1285 887 407 380 330 252 235 175
135 79 71
MspA1I CmG'CkG
Cuts at: 0 60 272 1168 1800 2075 2527 2536 2795
Size: 60 212 896 632 275 452 9 259
Cuts at: 2795 3009 3202 3620 3819 4236
Size: 214 193 418 199 417
Fragments arranged by size:
896 632 452 418 417 275 259 214
212 199 193 60 9
NaeI GCC'GGC
Cuts at: 0 2920 4236
Size: 2920 1316
NarI GG'CG_CC
Cuts at: 0 1259 2612 4236
Size: 1259 1353 1624
Fragments arranged by size:
1624 1353 1259
NdeI CA'TA_TG
Cuts at: 0 2846 4236
Size: 2846 1390
NgoMIV G'CCGG_C
Cuts at: 0 2918 4236
Size: 2918 1318
NheI G'CTAG_C
Cuts at: 0 906 4236
Size: 906 3330
NsiI A_TGCA'T
Cuts at: 0 2129 4236
Size: 2129 2107
NspI r_CATG'y
Cuts at: 0 2127 2962 4015 4236
Size: 2127 835 1053 221
Fragments arranged by size:
2127 1053 835 221
PasI CC'CwG_GG
Cuts at: 0 713 1906 4236
Size: 713 1193 2330
Fragments arranged by size:
2330 1193 713
PciI A'CATG_T
Cuts at: 0 4011 4236
Size: 4011 225
PfoI T'CCnGG_A
Cuts at: 0 845 1732 2576 4208 4236
Size: 845 887 844 1632 28
Fragments arranged by size:
1632 887 845 844 28
PmlI CAC'GTG
Cuts at: 0 3432 4236
Size: 3432 804
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3008 3040 4236
Size: 3008 32 1196
Fragments arranged by size:
3008 1196 32
PpuMI rG'GwC_Cy
Cuts at: 0 2151 4236
Size: 2151 2085
PshAI GACnn'nnGTC
Cuts at: 0 1149 4236
Size: 1149 3087
PspOMI G'GGCC_C
Cuts at: 0 19 3465 4236
Size: 19 3446 771
Fragments arranged by size:
3446 771 19
PspXI vC'TCGA_Gb
Cuts at: 0 1390 4236
Size: 1390 2846
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2191 2223 4236
Size: 2191 32 2013
Fragments arranged by size:
2191 2013 32
PstI C_TGCA'G
Cuts at: 0 905 2112 4236
Size: 905 1207 2124
Fragments arranged by size:
2124 1207 905
PvuII CAG'CTG
Cuts at: 0 60 272 1800 2527 2536 2795 3009 3202
Size: 60 212 1528 727 9 259 214 193
Cuts at: 3202 3819 4236
Size: 617 417
Fragments arranged by size:
1528 727 617 417 259 214 212 193
60 9
SacI G_AGCT'C
Cuts at: 0 3850 4236
Size: 3850 386
SapI GCTCTTCn'nnn_
Cuts at: 0 3545 4236
Size: 3545 691
ScaI AGT'ACT
Cuts at: 0 1534 4236
Size: 1534 2702
SexAI A'CCwGG_T
Cuts at: 0 1493 4236
Size: 1493 2743
SfcI C'TryA_G
Cuts at: 0 639 901 2108 4236
Size: 639 262 1207 2128
Fragments arranged by size:
2128 1207 639 262
SfoI GGC'GCC
Cuts at: 0 1260 2613 4236
Size: 1260 1353 1623
Fragments arranged by size:
1623 1353 1260
SmlI C'TyrA_G
Cuts at: 0 1390 3025 4236
Size: 1390 1635 1211
Fragments arranged by size:
1635 1390 1211
SphI G_CATG'C
Cuts at: 0 2127 2962 4236
Size: 2127 835 1274
Fragments arranged by size:
2127 1274 835
StuI AGG'CCT
Cuts at: 0 2382 4236
Size: 2382 1854
StyI C'CwwG_G
Cuts at: 0 24 1781 4236
Size: 24 1757 2455
Fragments arranged by size:
2455 1757 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 263 1919 2480 4088 4236
Size: 263 1656 561 1608 148
Fragments arranged by size:
1656 1608 561 263 148
TatI w'GTAC_w
Cuts at: 0 1532 2789 4236
Size: 1532 1257 1447
Fragments arranged by size:
1532 1447 1257
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1483 1515 2044 2076 3085 3117 4236
Size: 1483 32 529 32 1009 32 1119
Fragments arranged by size:
1483 1119 1009 529 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2710 4236
Size: 2710 1526
XbaI T'CTAG_A
Cuts at: 0 444 4236
Size: 444 3792
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2613 4236
Size: 2613 1623
XhoI C'TCGA_G
Cuts at: 0 1390 4236
Size: 1390 2846
XmnI GAAnn'nnTTC
Cuts at: 0 3373 4236
Size: 3373 863
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI CspCI DraI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I HincII HindIII HpaI KasI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NsiI NspI
PasI PciI PfoI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuII SacI SapI ScaI SexAI
SfcI SfoI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AhdI AloI AscI
AseI AsiSI AvrII BaeI BamHI BglI BsaBI BsiWI
BspEI BsrGI BssHII Bsu36I ClaI DrdI EcoNI EcoRV
FseI FspI FspAI KpnI MfeI MluI NcoI NotI
NruI PacI PflMI PmeI PsiI PvuI RsrII SacII
SalI SanDI SbfI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI XmaI ZraI