Restriction map for
pFN21AA1796
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BcgI
BciVI BfaI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiHKAI
BsiWI BsmAI BsmBI BsoBI BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MamI
MluI MluNI MroI MscI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 1068 GTCTAC GT^[AC][GT]AC
AccII 11 CGCG CG^CG
AflII 541 CTTAAG C^TTAAG
AflIII 184 ACGTGT A^C[GA][CT]GT
AlwNI 42 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 142 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 69 CCTCAGC CCTCAGC(-5/-2)
BclI 59 TGATCA T^GATCA
BcnI 99 CCGGG CC^[GC]GG
BfrI 541 CTTAAG C^TTAAG
BmyI 1026 GGGCAC G[AGT]GC[ACT]^C
BpmI 76 CTGGAG CTGGAG(16/14)
BseRI 52 GAGGAG GAGGAG(10/8)
BsiEI 96 CGACCG CG[GA][CT]^CG
BsmI 609 GAATGC GAATGC(1/-1)
Bsp1286I 1026 GGGCAC G[AGT]GC[ACT]^C
BspHI 1058 TCATGA T^CATGA
BstUI 11 CGCG CG^CG
BstXI 311 CCACAAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 536 GCATGC GC[ACGT]^[ACGT]GC
DraI 292 TTTAAA TTT^AAA
EcoT14I 648 CCATGG C^C[AT][AT]GG
FbaI 59 TGATCA T^GATCA
HaeII 893 AGCGCC [GA]GCGC^[CT]
HaeIII 81 GGCC GG^CC
HapII 99 CCGG C^CGG
HgaI 533 GACGC GACGC(5/10)
HpaII 99 CCGG C^CGG
MaeIII 188 GTGAC ^GT[ACGT]AC
MfeI 502 CAATTG C^AATTG
MspI 99 CCGG C^CGG
MunI 502 CAATTG C^AATTG
MvnI 11 CGCG CG^CG
MwoI 35 GCCCCACCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 99 CCGGG CC^[GC]GG
NcoI 648 CCATGG C^CATGG
NspI 536 GCATGC [GA]CATG^[CT]
PleI 190 GACTC GAGTC(4/5)
PstI 693 CTGCAG CTGCA^G
RcaI 1058 TCATGA T^CATGA
SfaNI 680 GCATC GCATC(5/9)
SfcI 693 CTGCAG C^T[GA][CT]AG
SmlI 541 CTTAAG C^T[CT][GA]AG
SphI 536 GCATGC GCATG^C
SspI 578 AATATT AAT^ATT
StyI 648 CCATGG C^C[AT][AT]GG
TaqI 594 TCGA T^CGA
Tsp45I 188 GTGAC ^GT[GC]AC
TthHB8I 594 TCGA T^CGA
XcmI 311 CCACAAAATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 142 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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BanI 6 GGTGCC G^G[CT][GA]CC
1027 GGCACC
BsgI 468 CTGCAC GTGCAG(16/14)
400 GTGCAG
BsmFI 101 GGGAC GGGAC(10/14)
806 GGGAC
Bsp1407I 821 TGTACA T^GTACA
937 TGTACA
BsrGI 821 TGTACA T^GTACA
937 TGTACA
BstF5I 303 GGATG GGATG(2/0)
978 GGATG
BstYI 1014 GGATCT [GA]^GATC[CT]
1089 AGATCC
CfoI 12 GCGC GCG^C
894 GCGC
DdeI 70 CTCAG C^T[ACGT]AG
914 CTAAG
EcoRI 463 GAATTC G^AATTC
703 GAATTC
FokI 303 GGATG GGATG(9/13)
978 GGATG
HhaI 12 GCGC GCG^C
894 GCGC
HinP1I 12 GCGC G^CGC
894 GCGC
MaeII 184 ACGT A^CGT
1125 ACGT
MflI 1014 GGATCT [GA]^GATC[CT]
1089 AGATCC
MslI 298 CACAAGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
604 CATGGGAATG
NlaIV 6 GGTGCC GG[ACGT]^[ACGT]CC
1027 GGCACC
SspBI 821 TGTACA T^GTACA
937 TGTACA
XhoII 1014 GGATCT [GA]^GATC[CT]
1089 AGATCC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
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AciI AcsI AfaI AluI AlwI
ApoI BbvI BsaJI BsiYI BslI
BsrI BssKI BstNI DpnII DsaI
EcoRII Fnu4HI HinfI HphI ItaI
MboI MboII MnlI MseI MspA1I
MvaI NdeII NlaIII PvuII RsaI
Sau3AI ScrFI TfiI Tru9I TseI
Tsp509I TspRI