Restriction map for
pFN21AA1796
(Circular) (Six-base) MAPSORT of: pFN21AA1796.seq Check: 7335 from: 1 to: 5833
pFN21AA1796 5833 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3643 278
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3074 3176 1937
Size: 1137 102 4594
Fragments arranged by size:
4594 1137 102
Acc65I G'GTAC_C
Cuts at: 3154 3154
Size: 5833
AclI AA'CG_TT
Cuts at: 1563 3960 4333 1563
Size: 2397 373 3063
Fragments arranged by size:
3063 2397 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2544 2677 3896 5280 5548 1632
Size: 912 133 1219 1384 268 1917
Fragments arranged by size:
1917 1384 1219 912 268 133
AflII C'TTAA_G
Cuts at: 820 1017 2546 820
Size: 197 1529 4107
Fragments arranged by size:
4107 1529 197
AflIII A'CryG_T
Cuts at: 1203 2189 3130 4732 5709 1203
Size: 986 941 1602 977 1327
Fragments arranged by size:
1602 1327 986 977 941
AgeI A'CCGG_T
Cuts at: 4664 4664
Size: 5833
AhdI GACnn_n'nnGTC
Cuts at: 4562 4562
Size: 5833
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5833
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 2270
Size: 32 5801
AlwNI CAG_nnn'CTG
Cuts at: 2052 3576 5148 2052
Size: 1524 1572 2737
Fragments arranged by size:
2737 1572 1524
ApaLI G'TGCA_C
Cuts at: 3890 5046 3890
Size: 1156 4677
ApoI r'AATT_y
Cuts at: 1493 2294 2468 2708 2885 2914 2930 3142 3368
Size: 801 174 240 177 29 16 212 226
Cuts at: 3368 3520 5629 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 801 240 226 212 177 174
152 29 16
AseI AT'TA_AT
Cuts at: 160 1032 4387 160
Size: 872 3355 1606
Fragments arranged by size:
3355 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
AvaI C'yCGr_G
Cuts at: 1870 1943 3158 1870
Size: 73 1215 4545
Fragments arranged by size:
4545 1215 73
BamHI G'GATC_C
Cuts at: 3163 3530 3163
Size: 367 5466
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2011 3032 3154 4609 618
Size: 325 217 627 224 1021 122 1455 1842
Fragments arranged by size:
1842 1455 1021 627 325 224 217 122
BanII G_rGCy'C
Cuts at: 1391 3152 1391
Size: 1761 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4862
BbvCI CC'TCA_GC
Cuts at: 2075 2075
Size: 5833
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4024 4058 1903
Size: 34 2087 34 3678
Fragments arranged by size:
3678 2087 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3725 4935 3725
Size: 1210 4623
BclI T'GATC_A
Cuts at: 1548 2064 1548
Size: 516 5317
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4444 136
Size: 107 122 71 631 605 2772 1525
Fragments arranged by size:
2772 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5828 5828
Size: 5833
BlpI GC'TnA_GC
Cuts at: 3250 3250
Size: 5833
Bme1580I G_kGCm'C
Cuts at: 1790 3035 3894 5050 1790
Size: 1245 859 1156 2573
Fragments arranged by size:
2573 1245 1156 859
BmgBI CAC'GTC
Cuts at: 1318 3131 1318
Size: 1813 4020
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3564 4522 442
Size: 416 53 83 399 24 2147 958 1753
Fragments arranged by size:
2147 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5833
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2102 4493 1239
Size: 863 2391 2579
Fragments arranged by size:
2579 2391 863
Bpu10I CC'TnA_GC
Cuts at: 2075 2075
Size: 5833
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3958 4823 5121 3958
Size: 865 298 4670
Fragments arranged by size:
4670 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4496 882
Size: 617 2997 2219
Fragments arranged by size:
2997 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3624 493
Size: 711 2420 2702
Fragments arranged by size:
2702 2420 711
BsaBI GATnn'nnATC
Cuts at: 5827 5827
Size: 5833
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3640 4022 4657 275
Size: 53 83 186 936 2107 382 635 1451
Fragments arranged by size:
2107 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4266 4664 4938 5085 5496 4266
Size: 398 274 147 411 4603
Fragments arranged by size:
4603 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 1178
Size: 30 165 30 867 30 4711
Fragments arranged by size:
4711 867 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 1186
Size: 886 4947
BseYI C'CCAG_C
Cuts at: 3098 5036 5532 3098
Size: 1938 496 3399
Fragments arranged by size:
3399 1938 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2426 2458 1163
Size: 573 690 32 4538
Fragments arranged by size:
4538 690 573 32
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2104 3283 4044 5072 1540
Size: 275 186 103 1179 761 1028 2301
Fragments arranged by size:
2301 1179 1028 761 275 186 103
BsiHKAI G_wGCw'C
Cuts at: 3894 3979 5050 3894
Size: 85 1071 4677
Fragments arranged by size:
4677 1071 85
BsmI GAATG_Cn'
Cuts at: 2620 3344 3437 2620
Size: 724 93 5016
Fragments arranged by size:
5016 724 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3035 3152 3894 3979 5050 1391
Size: 399 1245 117 742 85 1071 2174
Fragments arranged by size:
2174 1245 1071 742 399 117 85
BspEI T'CCGG_A
Cuts at: 5496 5496
Size: 5833
BspHI T'CATG_A
Cuts at: 3063 3722 5452 3063
Size: 659 1730 3444
Fragments arranged by size:
3444 1730 659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3188 844
Size: 2344 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3720 1394
Size: 429 1897 3507
Fragments arranged by size:
3507 1897 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4328 4502 65
Size: 1183 3080 174 1396
Fragments arranged by size:
3080 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4477 4664 1700
Size: 110 2667 187 2869
Fragments arranged by size:
2869 2667 187 110
BsrGI T'GTAC_A
Cuts at: 96 2826 2942 96
Size: 2730 116 2987
Fragments arranged by size:
2987 2730 116
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5833
BssSI C'ACGA_G
Cuts at: 3893 4905 3893
Size: 1012 4821
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5833
BstBI TT'CG_AA
Cuts at: 4637 4637
Size: 5833
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5833
BstXI CCAn_nnnn'nTGG
Cuts at: 2323 2323
Size: 5833
BstYI r'GATC_y
Cuts at: 1972 3019 3094 3163 3530 3915 3932 5384 5470
Size: 1047 75 69 367 385 17 1452 86
Cuts at: 5470 5482 5828 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1047 385 367 346 86 75
69 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2359 2653 3036 513
Size: 552 939 355 294 383 3310
Fragments arranged by size:
3310 939 552 383 355 294
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5094
Fragments arranged by size:
5094 683 56
BtsI GCAGTG_nn'
Cuts at: 2409 2829 3349 4143 4163 2409
Size: 420 520 794 20 4079
Fragments arranged by size:
4079 794 520 420 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5833
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2307 2342 529
Size: 35 1743 35 4020
Fragments arranged by size:
4020 1743 35 35
DraI TTT'AAA
Cuts at: 2299 3137 2299
Size: 838 4995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4840 809
Size: 507 3524 1802
Fragments arranged by size:
3524 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3280 4169 5798
Size: 54 1425 50 275 18 1450 889 1629
Cuts at: 5798 8
Size: 43
Fragments arranged by size:
1629 1450 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3280 1537
Size: 275 1468 4090
Fragments arranged by size:
4090 1468 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3763 1353
Size: 2410 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4420 4794 4940 387
Size: 1064 138 2831 374 146 1280
Fragments arranged by size:
2831 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2102 2544 2677 3896 4493 5280 5548
Size: 393 470 442 133 1219 597 787 268
Cuts at: 5548 1239
Size: 1524
Fragments arranged by size:
1524 1219 787 597 470 442 393 268
133
EcoRI G'AATT_C
Cuts at: 2468 2708 3142 3520 2468
Size: 240 434 378 4781
Fragments arranged by size:
4781 434 378 240
FspI TGC'GCA
Cuts at: 4339 4339
Size: 5833
HaeII r_GCGC'y
Cuts at: 1678 2902 4980 1678
Size: 1224 2078 2531
Fragments arranged by size:
2531 2078 1224
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2213 2245 4474 4506 4548
Size: 32 135 32 641 32 2229 32 42
Cuts at: 4548 4580 1373
Size: 32 2626
Fragments arranged by size:
2626 2229 641 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3177 3423 669
Size: 988 281 1239 246 3079
Fragments arranged by size:
3079 1239 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5833
HpaI GTT'AAC
Cuts at: 3423 3423
Size: 5833
KpnI G_GTAC'C
Cuts at: 3158 3158
Size: 5833
MfeI C'AATT_G
Cuts at: 2507 3432 2507
Size: 925 4908
MluI A'CGCG_T
Cuts at: 5709 5709
Size: 5833
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2493 3106 4947 5131 1305
Size: 1188 613 1841 184 2007
Fragments arranged by size:
2007 1841 1188 613 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4011
Fragments arranged by size:
4011 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2307 2613 3791 4150 4309 518
Size: 648 843 298 306 1178 359 159 2042
Fragments arranged by size:
2042 1178 843 648 359 306 298 159
MspA1I CmG'CkG
Cuts at: 1606 2095 2697 3102 3249 3926 5074 5319 1606
Size: 489 602 405 147 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 602 489 405 245 147
NcoI C'CATG_G
Cuts at: 513 1065 2004 2653 513
Size: 552 939 649 3693
Fragments arranged by size:
3693 939 649 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5833
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5833
NotI GC'GGCC_GC
Cuts at: 3280 3280
Size: 5833
NspI r_CATG'y
Cuts at: 1132 1229 2545 3191 4736 1132
Size: 97 1316 646 1545 2229
Fragments arranged by size:
2229 1545 1316 646 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5833
PciI A'CATG_T
Cuts at: 4732 4732
Size: 5833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5833
PmeI GTTT'AAAC
Cuts at: 3137 3137
Size: 5833
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2270 2302 3902 3934 5415 5447 2270
Size: 32 1600 32 1481 32 2656
Fragments arranged by size:
2656 1600 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5833
PsiI TTA'TAA
Cuts at: 3403 3403
Size: 5833
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2142 2174 2377 2409 2142
Size: 32 203 32 5566
Fragments arranged by size:
5566 203 32 32
PstI C_TGCA'G
Cuts at: 830 2702 3185 830
Size: 1872 483 3478
Fragments arranged by size:
3478 1872 483
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5833
PvuII CAG'CTG
Cuts at: 2095 2697 3102 2095
Size: 602 405 4826
Fragments arranged by size:
4826 602 405
SalI G'TCGA_C
Cuts at: 1936 3175 1936
Size: 1239 4594
SbfI CC_TGCA'GG
Cuts at: 3185 3185
Size: 5833
ScaI AGT'ACT
Cuts at: 4081 5793 4081
Size: 1712 4121
SfcI C'TryA_G
Cuts at: 826 1045 2698 3181 4316 4997 5188 826
Size: 219 1653 483 1135 681 191 1471
Fragments arranged by size:
1653 1471 1135 681 483 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5833
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5833
SmaI CCC'GGG
Cuts at: 1872 3160 1872
Size: 1288 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2546 3937 4838 5100 820
Size: 197 926 603 1391 901 262 1553
Fragments arranged by size:
1553 1391 926 901 603 262 197
SnaBI TAC'GTA
Cuts at: 493 3624 493
Size: 3131 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5833
SphI G_CATG'C
Cuts at: 1132 2545 3191 1132
Size: 1413 646 3774
Fragments arranged by size:
3774 1413 646
SspI AAT'ATT
Cuts at: 5 52 2585 3757 5
Size: 47 2533 1172 2081
Fragments arranged by size:
2533 2081 1172 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5833
StyI C'CwwG_G
Cuts at: 513 1065 2004 2653 3272 513
Size: 552 939 649 619 3074
Fragments arranged by size:
3074 939 649 619 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3860 3877 4030 4215 4660 325
Size: 1255 2280 17 153 185 445 1498
Fragments arranged by size:
2280 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2826 2942 4079
Size: 274 80 33 51 1443 849 116 1137
Cuts at: 4079 5791 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1137 849 274 138 116 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5793 5825 5793
Size: 32 5801
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5833
XbaI T'CTAG_A
Cuts at: 3169 3169
Size: 5833
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2323 2323
Size: 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5833
XmaI C'CCGG_G
Cuts at: 1870 3158 1870
Size: 1288 4545
XmnI GAAnn'nnTTC
Cuts at: 2151 3962 2151
Size: 1811 4022
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3641 276
Size: 53 83 186 936 2107 2468
Fragments arranged by size:
2468 2107 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI