Restriction map for
pFN21AA1796
(Linear) (Six-base) MAPSORT of: KIAA1796.seq Check: 2490 from: 1 to: 1128
KIAA1796.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AccI GT'mk_AC
Cuts at: 0 1069 1128
Size: 1069 59
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 672 1128
Size: 539 133 456
Fragments arranged by size:
539 456 133
AflII C'TTAA_G
Cuts at: 0 541 1128
Size: 541 587
AflIII A'CryG_T
Cuts at: 0 184 1128
Size: 184 944
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 1128
Size: 265 32 831
Fragments arranged by size:
831 265 32
AlwNI CAG_nnn'CTG
Cuts at: 0 47 1128
Size: 47 1081
ApoI r'AATT_y
Cuts at: 0 289 463 703 880 909 925 1128
Size: 289 174 240 177 29 16 203
Fragments arranged by size:
289 240 203 177 174 29 16
BanI G'GyrC_C
Cuts at: 0 6 1027 1128
Size: 6 1021 101
Fragments arranged by size:
1021 101 6
BbvCI CC'TCA_GC
Cuts at: 0 70 1128
Size: 70 1058
BclI T'GATC_A
Cuts at: 0 59 1128
Size: 59 1069
Bme1580I G_kGCm'C
Cuts at: 0 1030 1128
Size: 1030 98
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1128
Size: 97 1031
Bpu10I CC'TnA_GC
Cuts at: 0 70 1128
Size: 70 1058
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 1128
Size: 265 30 833
Fragments arranged by size:
833 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 1128
Size: 67 1061
BseYI C'CCAG_C
Cuts at: 0 1093 1128
Size: 1093 35
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 453 1128
Size: 421 32 675
Fragments arranged by size:
675 421 32
BsiEI CG_ry'CG
Cuts at: 0 99 1128
Size: 99 1029
BsmI GAATG_Cn'
Cuts at: 0 615 1128
Size: 615 513
Bsp1286I G_dGCh'C
Cuts at: 0 1030 1128
Size: 1030 98
BspHI T'CATG_A
Cuts at: 0 1058 1128
Size: 1058 70
BsrGI T'GTAC_A
Cuts at: 0 821 937 1128
Size: 821 116 191
Fragments arranged by size:
821 191 116
BstXI CCAn_nnnn'nTGG
Cuts at: 0 318 1128
Size: 318 810
BstYI r'GATC_y
Cuts at: 0 1014 1089 1128
Size: 1014 75 39
Fragments arranged by size:
1014 75 39
BtgI C'CryG_G
Cuts at: 0 354 648 1031 1128
Size: 354 294 383 97
Fragments arranged by size:
383 354 294 97
BtsI GCAGTG_nn'
Cuts at: 0 404 824 1128
Size: 404 420 304
Fragments arranged by size:
420 404 304
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 302 337 1128
Size: 302 35 791
Fragments arranged by size:
791 302 35
DraI TTT'AAA
Cuts at: 0 294 1128
Size: 294 834
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 539 672 1128
Size: 97 442 133 456
Fragments arranged by size:
456 442 133 97
EcoRI G'AATT_C
Cuts at: 0 463 703 1128
Size: 463 240 425
Fragments arranged by size:
463 425 240
HaeII r_GCGC'y
Cuts at: 0 897 1128
Size: 897 231
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 1128
Size: 208 32 888
Fragments arranged by size:
888 208 32
MfeI C'AATT_G
Cuts at: 0 502 1128
Size: 502 626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 1101 1128
Size: 488 613 27
Fragments arranged by size:
613 488 27
MslI CAynn'nnrTG
Cuts at: 0 302 608 1128
Size: 302 306 520
Fragments arranged by size:
520 306 302
MspA1I CmG'CkG
Cuts at: 0 90 692 1097 1128
Size: 90 602 405 31
Fragments arranged by size:
602 405 90 31
NcoI C'CATG_G
Cuts at: 0 648 1128
Size: 648 480
NspI r_CATG'y
Cuts at: 0 540 1128
Size: 540 588
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 1128
Size: 265 32 831
Fragments arranged by size:
831 265 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 137 169 372 404 1128
Size: 137 32 203 32 724
Fragments arranged by size:
724 203 137 32 32
PstI C_TGCA'G
Cuts at: 0 697 1128
Size: 697 431
PvuII CAG'CTG
Cuts at: 0 90 692 1097 1128
Size: 90 602 405 31
Fragments arranged by size:
602 405 90 31
SfcI C'TryA_G
Cuts at: 0 693 1128
Size: 693 435
SmlI C'TyrA_G
Cuts at: 0 541 1128
Size: 541 587
SphI G_CATG'C
Cuts at: 0 540 1128
Size: 540 588
SspI AAT'ATT
Cuts at: 0 580 1128
Size: 580 548
StyI C'CwwG_G
Cuts at: 0 648 1128
Size: 648 480
TatI w'GTAC_w
Cuts at: 0 821 937 1128
Size: 821 116 191
Fragments arranged by size:
821 191 116
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 1128
Size: 318 810
XmnI GAAnn'nnTTC
Cuts at: 0 146 1128
Size: 146 982
Enzymes that do cut:
AccI AcuI AflII AflIII AloI AlwNI ApoI BanI
BbvCI BclI Bme1580I BpmI Bpu10I BsaXI BseRI BseYI
BsgI BsiEI BsmI Bsp1286I BspHI BsrGI BstXI BstYI
BtgI BtsI CspCI DraI Eco57MI EcoRI HaeII Hin4I
MfeI MmeI MslI MspA1I NcoI NspI PpiI PsrI
PstI PvuII SfcI SmlI SphI SspI StyI TatI
XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbsI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsiHKAI BsiWI BsmBI
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraIII DrdI
EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI StuI SwaI TaqII TstI Tth111I XbaI XhoI
XmaI ZraI