(Circular) (Six-base) MAPSORT of: pFN21AA1795.seq Check: 9002 from: 1 to: 6004 pFN21AA1795 6004 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2960 3347 1937 Size: 1023 387 4594 Fragments arranged by size: 4594 1023 387 Acc65I G'GTAC_C Cuts at: 2392 3325 2392 Size: 933 5071 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4067 5451 5719 1632 Size: 2435 1384 268 1917 Fragments arranged by size: 2435 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 4903 5880 1203 Size: 3700 977 1327 Fragments arranged by size: 3700 1327 977 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6004 AlwNI CAG_nnn'CTG Cuts at: 2951 3747 5319 2951 Size: 796 1572 3636 Fragments arranged by size: 3636 1572 796 ApaLI G'TGCA_C Cuts at: 2643 4061 5217 2643 Size: 1418 1156 3430 Fragments arranged by size: 3430 1418 1156 ApoI r'AATT_y Cuts at: 1493 3313 3539 3691 5800 1493 Size: 1820 226 152 2109 1697 Fragments arranged by size: 2109 1820 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3212 4558 160 Size: 872 2180 1346 1606 Fragments arranged by size: 2180 1606 1346 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 3329 1870 Size: 73 1386 4545 Fragments arranged by size: 4545 1386 73 BamHI G'GATC_C Cuts at: 3334 3701 3334 Size: 367 5637 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2392 3325 4780 618 Size: 325 217 627 605 933 1455 1842 Fragments arranged by size: 1842 1455 933 627 605 325 217 BanII G_rGCy'C Cuts at: 1391 2945 3323 1391 Size: 1554 378 4072 Fragments arranged by size: 4072 1554 378 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5033 BbvCI CC'TCA_GC Cuts at: 2615 2615 Size: 6004 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4195 4229 1903 Size: 34 50 34 2174 34 3678 Fragments arranged by size: 3678 2174 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 2815 1548 Size: 1267 4737 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2588 4615 136 Size: 107 122 71 631 605 916 2027 1525 Fragments arranged by size: 2027 1525 916 631 605 122 107 71 BglII A'GATC_T Cuts at: 5999 5999 Size: 6004 BlpI GC'TnA_GC Cuts at: 2162 2952 3421 2162 Size: 790 469 4745 Fragments arranged by size: 4745 790 469 Bme1580I G_kGCm'C Cuts at: 1790 2647 3179 4065 5221 1790 Size: 857 532 886 1156 2573 Fragments arranged by size: 2573 1156 886 857 532 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6004 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3735 4693 442 Size: 416 53 83 399 24 2318 958 1753 Fragments arranged by size: 2318 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2322 2322 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2100 2292 2302 3086 3170 4664 1239 Size: 861 192 10 784 84 1494 2579 Fragments arranged by size: 2579 1494 861 784 192 84 10 Bpu10I CC'TnA_GC Cuts at: 2297 2615 2297 Size: 318 5686 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2310 4129 4994 5292 2310 Size: 1819 865 298 3022 Fragments arranged by size: 3022 1819 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2341 2994 4667 882 Size: 617 842 653 1673 2219 Fragments arranged by size: 2219 1673 842 653 617 BsaAI yAC'GTr Cuts at: 493 1204 3795 493 Size: 711 2591 2702 Fragments arranged by size: 2702 2591 711 BsaBI GATnn'nnATC Cuts at: 5998 5998 Size: 6004 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3811 4193 4828 275 Size: 53 83 186 936 2278 382 635 1451 Fragments arranged by size: 2278 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4437 4835 5109 5256 5667 4437 Size: 398 274 147 411 4774 Fragments arranged by size: 4774 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2113 2143 2993 3023 1178 Size: 30 165 30 710 30 850 30 4159 Fragments arranged by size: 4159 850 710 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6004 BseYI C'CCAG_C Cuts at: 2069 2351 3172 5207 5703 2069 Size: 282 821 2035 496 2370 Fragments arranged by size: 2370 2035 821 496 282 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5431 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2163 2647 4065 4150 5221 2163 Size: 484 1418 85 1071 2946 Fragments arranged by size: 2946 1418 1071 484 85 BsmI GAATG_Cn' Cuts at: 3515 3608 3515 Size: 93 5911 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2163 2647 2945 3179 3323 4065 4150 Size: 399 373 484 298 234 144 742 85 Cuts at: 4150 5221 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 484 399 373 298 234 144 85 BspEI T'CCGG_A Cuts at: 5667 5667 Size: 6004 BspHI T'CATG_A Cuts at: 3893 5623 3893 Size: 1730 4274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3359 844 Size: 2515 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3891 1394 Size: 429 2068 3507 Fragments arranged by size: 3507 2068 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4499 4673 65 Size: 1183 3251 174 1396 Fragments arranged by size: 3251 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4648 4835 1700 Size: 110 2838 187 2869 Fragments arranged by size: 2869 2838 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6004 BssHII G'CGCG_C Cuts at: 1925 2862 1925 Size: 937 5067 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 3281 794 Size: 2487 3517 BstBI TT'CG_AA Cuts at: 4808 4808 Size: 6004 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstYI r'GATC_y Cuts at: 1972 2788 3256 3334 3701 4086 4103 5555 5641 Size: 816 468 78 367 385 17 1452 86 Cuts at: 5641 5653 5999 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 816 468 385 367 346 86 78 17 12 BtgI C'CryG_G Cuts at: 513 1065 2133 513 Size: 552 1068 4384 Fragments arranged by size: 4384 1068 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5265 Fragments arranged by size: 5265 683 56 BtsI GCAGTG_nn' Cuts at: 3520 4314 4334 3520 Size: 794 20 5190 Fragments arranged by size: 5190 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3063 3098 529 Size: 35 2499 35 3435 Fragments arranged by size: 3435 2499 35 35 DraI TTT'AAA Cuts at: 2763 3308 2763 Size: 545 5459 DraIII CAC_nnn'GTG Cuts at: 3281 3281 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2207 5011 809 Size: 507 891 2804 1802 Fragments arranged by size: 2804 1802 891 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3451 4340 5969 Size: 54 1425 50 275 18 1621 889 1629 Cuts at: 5969 8 Size: 43 Fragments arranged by size: 1629 1621 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3934 1353 Size: 2581 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4591 4965 5111 387 Size: 1064 138 3002 374 146 1280 Fragments arranged by size: 3002 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2100 2292 2302 3086 3170 4067 4664 Size: 393 468 192 10 784 84 897 597 Cuts at: 4664 5451 5719 1239 Size: 787 268 1524 Fragments arranged by size: 1524 897 787 784 597 468 393 268 192 84 10 EcoO109I rG'GnC_Cy Cuts at: 2065 2065 Size: 6004 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 5151 1678 Size: 3473 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2174 2206 2993 3025 4645 Size: 32 135 32 602 32 787 32 1620 Cuts at: 4645 4677 4719 4751 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1620 787 602 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3348 3594 669 Size: 988 281 1410 246 3079 Fragments arranged by size: 3079 1410 988 281 246 HindIII A'AGCT_T Cuts at: 748 2404 748 Size: 1656 4348 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 2396 3329 2396 Size: 933 5071 MfeI C'AATT_G Cuts at: 2507 3603 2507 Size: 1096 4908 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2044 3201 5118 5302 1305 Size: 739 1157 1917 184 2007 Fragments arranged by size: 2007 1917 1157 739 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4182 Fragments arranged by size: 4182 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3962 4321 4480 518 Size: 648 2796 359 159 2042 Fragments arranged by size: 2796 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2519 3420 4097 5245 5490 1606 Size: 913 901 677 1148 245 2120 Fragments arranged by size: 2120 1148 913 901 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5452 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 2917 3362 4907 1132 Size: 97 1688 445 1545 2229 Fragments arranged by size: 2229 1688 1545 445 97 PasI CC'CwG_GG Cuts at: 1348 3262 1348 Size: 1914 4090 PciI A'CATG_T Cuts at: 4903 4903 Size: 6004 PflMI CCAn_nnn'nTGG Cuts at: 1108 2282 1108 Size: 1174 4830 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2065 2065 Size: 6004 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PstI C_TGCA'G Cuts at: 830 2478 3356 830 Size: 1648 878 3478 Fragments arranged by size: 3478 1648 878 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 PvuII CAG'CTG Cuts at: 2519 2519 Size: 6004 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 2323 4252 5964 2323 Size: 1929 1712 2363 Fragments arranged by size: 2363 1929 1712 SfcI C'TryA_G Cuts at: 826 1045 2474 2754 2827 3352 4487 5168 5359 Size: 219 1429 280 73 525 1135 681 191 Cuts at: 5359 826 Size: 1471 Fragments arranged by size: 1471 1429 1135 681 525 280 219 191 73 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 3331 1872 Size: 1459 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2325 4108 5009 5271 820 Size: 197 926 382 1783 901 262 1553 Fragments arranged by size: 1783 1553 926 901 382 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SspI AAT'ATT Cuts at: 5 52 2568 3928 5 Size: 47 2516 1360 2081 Fragments arranged by size: 2516 2081 1360 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6004 StyI C'CwwG_G Cuts at: 513 1065 2527 3443 513 Size: 552 1462 916 3074 Fragments arranged by size: 3074 1462 916 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4031 4048 4201 4386 4831 325 Size: 1255 2451 17 153 185 445 1498 Fragments arranged by size: 2451 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2248 2321 2820 Size: 274 80 33 51 1443 271 73 499 Cuts at: 2820 3048 3143 3153 4250 5962 96 Size: 228 95 10 1097 1712 138 Fragments arranged by size: 1712 1443 1097 499 274 271 228 138 95 80 73 51 33 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2113 2145 5964 5996 2113 Size: 32 3819 32 2121 Fragments arranged by size: 3819 2121 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6004 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 3329 1870 Size: 1459 4545 XmnI GAAnn'nnTTC Cuts at: 4133 4133 Size: 6004 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI