(Circular) (Six-base) MAPSORT of: pFN21AA1782.seq Check: 2515 from: 1 to: 6319 pFN21AA1782 6319 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3116 3116 Size: 6319 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2352 4129 278 Size: 53 83 186 936 816 1777 2468 Fragments arranged by size: 2468 1777 936 816 186 83 53 AccI GT'mk_AC Cuts at: 1937 3069 3097 3662 1937 Size: 1132 28 565 4594 Fragments arranged by size: 4594 1132 565 28 Acc65I G'GTAC_C Cuts at: 3640 3640 Size: 6319 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2721 2863 3508 4382 5766 6034 1632 Size: 1089 142 645 874 1384 268 1917 Fragments arranged by size: 1917 1384 1089 874 645 268 142 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 5218 6195 1203 Size: 4015 977 1327 Fragments arranged by size: 4015 1327 977 AgeI A'CCGG_T Cuts at: 3570 5150 3570 Size: 1580 4739 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 2409 3317 1166 Size: 1243 908 4168 Fragments arranged by size: 4168 1243 908 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2339 3354 1184 Size: 1155 1015 4149 Fragments arranged by size: 4149 1155 1015 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2057 2089 2057 Size: 32 6287 AlwNI CAG_nnn'CTG Cuts at: 4062 5634 4062 Size: 1572 4747 ApaI G_GGCC'C Cuts at: 2086 2097 2086 Size: 11 6308 ApaLI G'TGCA_C Cuts at: 2390 4376 5532 2390 Size: 1986 1156 3177 Fragments arranged by size: 3177 1986 1156 ApoI r'AATT_y Cuts at: 1493 3578 3628 3854 4006 6115 1493 Size: 2085 50 226 152 2109 1697 Fragments arranged by size: 2109 2085 1697 226 152 50 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2078 2911 3117 3177 3644 1870 Size: 73 135 833 206 60 467 4545 Fragments arranged by size: 4545 833 467 206 135 73 60 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3454 3487 3454 Size: 33 6286 BamHI G'GATC_C Cuts at: 3113 3649 4016 3113 Size: 536 367 5416 Fragments arranged by size: 5416 536 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2441 2670 2755 3386 3640 Size: 325 217 627 654 229 85 631 254 Cuts at: 3640 5095 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 654 631 627 325 254 229 217 85 BanII G_rGCy'C Cuts at: 1391 2086 2097 2268 3638 1391 Size: 695 11 171 1370 4072 Fragments arranged by size: 4072 1370 695 171 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2022 928 Size: 971 123 5225 Fragments arranged by size: 5225 971 123 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4510 4544 1903 Size: 34 2573 34 3678 Fragments arranged by size: 3678 2573 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3286 3482 4211 5421 3286 Size: 196 729 1210 4184 Fragments arranged by size: 4184 1210 729 196 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4930 136 Size: 107 122 71 631 605 3258 1525 Fragments arranged by size: 3258 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6314 6314 Size: 6319 BlpI GC'TnA_GC Cuts at: 3736 3736 Size: 6319 Bme1580I G_kGCm'C Cuts at: 1790 2086 2097 2394 3389 4380 5536 1790 Size: 296 11 297 995 991 1156 2573 Fragments arranged by size: 2573 1156 995 991 297 296 11 BmgBI CAC'GTC Cuts at: 1318 3490 1318 Size: 2172 4147 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2081 4050 5008 Size: 416 53 83 399 24 664 1969 958 Cuts at: 5008 442 Size: 1753 Fragments arranged by size: 1969 1753 958 664 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2320 3179 2320 Size: 859 5460 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2672 2768 3122 4979 1239 Size: 845 588 96 354 1857 2579 Fragments arranged by size: 2579 1857 845 588 354 96 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2324 4444 5309 5607 2324 Size: 2120 865 298 3036 Fragments arranged by size: 3036 2120 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3522 4982 882 Size: 617 2023 1460 2219 Fragments arranged by size: 2219 2023 1460 617 BsaAI yAC'GTr Cuts at: 493 1204 2741 4110 493 Size: 711 1537 1369 2702 Fragments arranged by size: 2702 1537 1369 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2349 4126 4508 5143 Size: 53 83 186 936 816 1777 382 635 Cuts at: 5143 275 Size: 1451 Fragments arranged by size: 1777 1451 936 816 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3570 4752 5150 5424 5571 5982 3570 Size: 1182 398 274 147 411 3907 Fragments arranged by size: 3907 1182 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2057 2087 2564 2594 1178 Size: 30 165 30 654 30 477 30 4903 Fragments arranged by size: 4903 654 477 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2198 2572 2669 3155 1186 Size: 1012 374 97 486 4350 Fragments arranged by size: 4350 1012 486 374 97 BseYI C'CCAG_C Cuts at: 2200 3209 3300 5522 6018 2200 Size: 1009 91 2222 496 2501 Fragments arranged by size: 2501 2222 1009 496 91 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2471 2756 3015 1163 Size: 573 735 285 259 4467 Fragments arranged by size: 4467 735 573 285 259 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2806 3769 4530 5558 1540 Size: 275 186 805 963 761 1028 2301 Fragments arranged by size: 2301 1028 963 805 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2108 2385 2394 2661 3465 4380 4465 5536 2108 Size: 277 9 267 804 915 85 1071 2891 Fragments arranged by size: 2891 1071 915 804 277 267 85 9 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2086 2097 2108 2268 2385 2394 2661 Size: 399 296 11 11 160 117 9 267 Cuts at: 2661 3389 3465 3638 4380 4465 5536 1391 Size: 728 76 173 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 728 399 296 267 173 160 117 85 76 11 11 9 BspEI T'CCGG_A Cuts at: 5982 5982 Size: 6319 BspHI T'CATG_A Cuts at: 4208 5938 4208 Size: 1730 4589 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2475 3116 3674 844 Size: 1631 641 558 3489 Fragments arranged by size: 3489 1631 641 558 BsrBI CCG'CTC Cuts at: 1394 1823 2031 2139 2215 2668 2788 4206 1394 Size: 429 208 108 76 453 120 1418 3507 Fragments arranged by size: 3507 1418 453 429 208 120 108 76 BsrDI GCAATG_nn' Cuts at: 65 1248 2029 2425 3220 4814 4988 65 Size: 1183 781 396 795 1594 174 1396 Fragments arranged by size: 1594 1396 1183 795 781 396 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2538 3171 3327 3570 4963 5150 1700 Size: 110 728 633 156 243 1393 187 2869 Fragments arranged by size: 2869 1393 728 633 243 187 156 110 BsrGI T'GTAC_A Cuts at: 96 2172 96 Size: 2076 4243 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 3219 794 Size: 2425 3894 BstBI TT'CG_AA Cuts at: 5123 5123 Size: 6319 BstEII G'GTnAC_C Cuts at: 1184 2558 1184 Size: 1374 4945 BstXI CCAn_nnnn'nTGG Cuts at: 2164 3319 3322 2164 Size: 1155 3 5161 Fragments arranged by size: 5161 1155 3 BstYI r'GATC_y Cuts at: 1972 3113 3649 4016 4401 4418 5870 5956 5968 Size: 1141 536 367 385 17 1452 86 12 Cuts at: 5968 6314 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1141 536 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3138 3285 3138 Size: 147 6172 BtgI C'CryG_G Cuts at: 513 1065 2004 3517 513 Size: 552 939 1513 3315 Fragments arranged by size: 3315 1513 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2193 487 Size: 683 56 967 4613 Fragments arranged by size: 4613 967 683 56 BtsI GCAGTG_nn' Cuts at: 3463 3835 4629 4649 3463 Size: 372 794 20 5133 Fragments arranged by size: 5133 794 372 20 ClaI AT'CG_AT Cuts at: 1554 2802 1554 Size: 1248 5071 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2419 2454 3532 3567 529 Size: 35 1855 35 1078 35 3281 Fragments arranged by size: 3281 1855 1078 35 35 35 DraI TTT'AAA Cuts at: 2509 3623 2509 Size: 1114 5205 DraIII CAC_nnn'GTG Cuts at: 2147 2147 Size: 6319 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5326 809 Size: 507 4010 1802 Fragments arranged by size: 4010 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2026 2623 2719 Size: 54 1425 50 275 18 196 597 96 Cuts at: 2719 2789 2811 3766 4655 6284 8 Size: 70 22 955 889 1629 43 Fragments arranged by size: 1629 1425 955 889 597 275 196 96 70 54 50 43 22 18 EagI C'GGCC_G Cuts at: 1537 1812 3766 1537 Size: 275 1954 4090 Fragments arranged by size: 4090 1954 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2131 3482 3589 4249 1353 Size: 778 1351 107 660 3423 Fragments arranged by size: 3423 1351 778 660 107 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3005 4906 5280 5426 387 Size: 1064 138 1416 1901 374 146 1280 Fragments arranged by size: 1901 1416 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2672 2721 2768 2863 3122 3508 Size: 393 452 588 49 47 95 259 386 Cuts at: 3508 4382 4979 5766 6034 1239 Size: 874 597 787 268 1524 Fragments arranged by size: 1524 874 787 597 588 452 393 386 268 259 95 49 47 EcoNI CCTnn'n_nnAGG Cuts at: 2225 3284 2225 Size: 1059 5260 EcoO109I rG'GnC_Cy Cuts at: 2082 2083 2093 2094 2418 3134 3142 3158 3182 Size: 1 10 1 324 716 8 16 24 Cuts at: 3182 3290 3355 3395 2082 Size: 108 65 40 5006 Fragments arranged by size: 5006 716 324 108 65 40 24 16 10 8 1 1 EcoRI G'AATT_C Cuts at: 3578 3628 4006 3578 Size: 50 378 5891 Fragments arranged by size: 5891 378 50 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 5466 1678 Size: 3788 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 2243 2275 2894 Size: 32 135 32 599 32 40 32 619 Cuts at: 2894 2926 4960 4992 5034 5066 1373 Size: 32 2034 32 42 32 2626 Fragments arranged by size: 2626 2034 619 599 135 42 40 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3098 3663 3909 669 Size: 988 281 1160 565 246 3079 Fragments arranged by size: 3079 1160 988 565 281 246 HindIII A'AGCT_T Cuts at: 748 2709 748 Size: 1961 4358 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KpnI G_GTAC'C Cuts at: 3644 3644 Size: 6319 MfeI C'AATT_G Cuts at: 3031 3918 3031 Size: 887 5432 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5433 5617 1305 Size: 4128 184 2007 Fragments arranged by size: 4128 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2721 2813 10 Size: 54 1425 343 889 92 3516 Fragments arranged by size: 3516 1425 889 343 92 54 MslI CAynn'nnrTG Cuts at: 518 1166 2162 2409 3077 3317 3320 4277 4636 Size: 648 996 247 668 240 3 957 359 Cuts at: 4636 4795 518 Size: 159 2042 Fragments arranged by size: 2042 996 957 668 648 359 247 240 159 3 MspA1I CmG'CkG Cuts at: 1606 3735 4412 5560 5805 1606 Size: 2129 677 1148 245 2120 Fragments arranged by size: 2129 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2540 3329 2540 Size: 789 5530 NcoI C'CATG_G Cuts at: 513 1065 2004 3517 513 Size: 552 939 1513 3315 Fragments arranged by size: 3315 1513 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NgoMIV G'CCGG_C Cuts at: 2538 3327 2538 Size: 789 5530 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 3677 5222 1132 Size: 97 2448 1545 2229 Fragments arranged by size: 2448 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2269 2309 3197 1348 Size: 921 40 888 4470 Fragments arranged by size: 4470 921 888 40 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 2713 1108 Size: 1605 4714 PfoI T'CCnGG_A Cuts at: 2220 3109 3480 2220 Size: 889 371 5059 Fragments arranged by size: 5059 889 371 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2057 2089 4388 4420 5901 5933 2057 Size: 32 2299 32 1481 32 2443 Fragments arranged by size: 2443 2299 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3134 3142 3158 3290 3355 3134 Size: 8 16 132 65 6098 Fragments arranged by size: 6098 132 65 16 8 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PspOMI G'GGCC_C Cuts at: 2082 2093 2082 Size: 11 6308 PstI C_TGCA'G Cuts at: 830 3671 830 Size: 2841 3478 PvuI CG_AT'CG Cuts at: 2001 2806 2001 Size: 805 5514 SalI G'TCGA_C Cuts at: 1936 3096 3661 1936 Size: 1160 565 4594 Fragments arranged by size: 4594 1160 565 SanDI GG'GwC_CC Cuts at: 3290 3290 Size: 6319 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SexAI A'CCwGG_T Cuts at: 2725 2725 Size: 6319 SfcI C'TryA_G Cuts at: 826 1045 2684 3667 4802 5483 5674 826 Size: 219 1639 983 1135 681 191 1471 Fragments arranged by size: 1639 1471 1135 983 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 2538 1700 Size: 838 5481 SmaI CCC'GGG Cuts at: 1872 3646 1872 Size: 1774 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2339 4423 5324 5586 820 Size: 197 926 396 2084 901 262 1553 Fragments arranged by size: 2084 1553 926 901 396 262 197 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3677 1132 Size: 2545 3774 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 3449 1517 Size: 1932 4387 StyI C'CwwG_G Cuts at: 513 1065 2004 3402 3517 3758 513 Size: 552 939 1398 115 241 3074 Fragments arranged by size: 3074 1398 939 552 241 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2468 2981 3040 3089 4346 4363 4516 Size: 1255 888 513 59 49 1257 17 153 Cuts at: 4516 4701 5146 325 Size: 185 445 1498 Fragments arranged by size: 1498 1257 1255 888 513 445 185 153 59 49 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2172 4565 6277 Size: 274 80 33 51 1443 195 2393 1712 Cuts at: 6277 96 Size: 138 Fragments arranged by size: 2393 1712 1443 274 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2364 2396 2564 2596 6279 6311 2364 Size: 32 168 32 3683 32 2372 Fragments arranged by size: 3683 2372 168 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3488 1316 Size: 2172 4147 XbaI T'CTAG_A Cuts at: 3655 3655 Size: 6319 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2383 2714 3319 2383 Size: 331 605 5383 Fragments arranged by size: 5383 605 331 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 3644 1870 Size: 1774 4545 XmnI GAAnn'nnTTC Cuts at: 2072 3411 3449 4448 2072 Size: 1339 38 999 3943 Fragments arranged by size: 3943 1339 999 38 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2350 4127 276 Size: 53 83 186 936 816 1777 2468 Fragments arranged by size: 2468 1777 936 816 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SfiI SfoI SrfI SwaI