Restriction map for
pFN21AA1739
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AgeI AhdI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BbrPI BclI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrGI
BssHII Bst1107I BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SfuI SnaBI SpeI
SspBI SspI StuI SwaI TaqI
TfiI Tsp509I TthHB8I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AccI 703 GTCTAC GT^[AC][GT]AC
AflII 2227 CTTAAG C^TTAAG
AflIII 968 ACATGT A^C[GA][CT]GT
Alw44I 408 GTGCAC G^TGCAC
ApaLI 408 GTGCAC G^TGCAC
BamHI 2193 GGATCC G^GATCC
BbvCI 61 GCTGAGG CCTCAGC(-5/-2)
BciVI 296 GTATCC GTATCC(6/5)
BfaI 2380 CTAG C^TAG
BfrI 2227 CTTAAG C^TTAAG
BsiEI 1275 CGACCG CG[GA][CT]^CG
BspLU11I 968 ACATGT A^CATGT
BsrDI 2054 CATTGC GCAATG(2/0)
BstEII 1950 GGTGACC G^GT[ACGT]ACC
BstPI 1950 GGTGACC G^GT[ACGT]ACC
DraIII 1264 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 694 GACGACAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1377 CCTGATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1950 GGTGACC G^GT[ACGT]ACC
MaeI 2380 CTAG C^TAG
SacI 2422 GAGCTC GAGCT^C
SapI 2074 GAAGAGC GCTCTTC(1/4)
SbfI 906 CCTGCAGG CCTGCA^GG
SfiI 496 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2059 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 906 CCTGCAGG CCTGCA^GG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AcyI 202 GGCGCC G[GA]^CG[CT]C
1127 GGCGCC
AspHI 408 GTGCAC G[AT]GC[AT]^C
2422 GAGCTC
BalI 113 TGGCCA TGG^CCA
1590 TGGCCA
BbeI 202 GGCGCC GGCGC^C
1127 GGCGCC
BcgI 738 CGACTGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1939 GCAGGCCCGTCG
BsaAI 196 TACGTG [CT]AC^GT[GA]
2365 TACGTG
BsaHI 202 GGCGCC G[GA]^CG[CT]C
1127 GGCGCC
BsgI 1477 GTGCAG GTGCAG(16/14)
1517 GTGCAG
BsiHKAI 408 GTGCAC G[AT]GC[AT]^C
2422 GAGCTC
BsmAI 1331 GAGAC GTCTC(1/5)
2439 GAGAC
BsmBI 1331 GAGACG CGTCTC(1/5)
2439 GAGACG
BsrBI 1464 CCGCTC CCGCTC(-3/-3)
721 GAGCGG
BstXI 1142 CCAGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2256 CCAGCCCGGTGG
EarI 1003 GAAGAG CTCTTC(1/4)
2074 GAAGAG
HaeII 202 GGCGCC [GA]GCGC^[CT]
1127 GGCGCC
Hin1I 202 GGCGCC G[GA]^CG[CT]C
1127 GGCGCC
HincII 418 GTCAAC GT[CT]^[GA]AC
1561 GTCAAC
HindII 418 GTCAAC GT[CT]^[GA]AC
1561 GTCAAC
KasI 202 GGCGCC G^GCGCC
1127 GGCGCC
Ksp632I 1003 GAAGAG CTCTTC(1/4)
2074 GAAGAG
MluNI 113 TGGCCA TGG^CCA
1590 TGGCCA
MscI 113 TGGCCA TGG^CCA
1590 TGGCCA
MseI 1112 TTAA T^TAA
2228 TTAA
MslI 1692 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1695 CACGGTGGTG
NaeI 1456 GCCGGC GCC^GGC
2060 GCCGGC
NarI 202 GGCGCC GG^CGCC
1127 GGCGCC
NgoMIV 1456 GCCGGC G^CCGGC
2060 GCCGGC
PflMI 1263 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1827 CCAGGCCCTGG
SexAI 1265 ACCTGGT A^CC[AT]GGT
1715 ACCTGGT
SfoI 202 GGCGCC GGC^GCC
1127 GGCGCC
SmlI 1345 CTCAAG C^T[CT][GA]AG
2227 CTTAAG
SphI 850 GCATGC GCATG^C
2244 GCATGC
Tru9I 1112 TTAA T^TAA
2228 TTAA
Tsp45I 965 GTGAC ^GT[GC]AC
1951 GTGAC
Van91I 1263 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1827 CCAGGCCCTGG
XcmI 1412 CCATGAGAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1568 CCAGCCGCTGTGTGG
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StyI TseI
TspRI Tth111I XhoII XmaCI XmaI