(Circular) (Six-base) MAPSORT of: pFN21AA1739.seq Check: 5181 from: 1 to: 7204 pFN21AA1739 7204 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2749 3202 2749 Size: 453 6751 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5014 278 Size: 53 83 186 936 3478 2468 Fragments arranged by size: 3478 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2709 4547 1937 Size: 772 1838 4594 Fragments arranged by size: 4594 1838 772 Acc65I G'GTAC_C Cuts at: 4525 4525 Size: 7204 AclI AA'CG_TT Cuts at: 1563 5331 5704 1563 Size: 3768 373 3063 Fragments arranged by size: 3768 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2215 2704 2861 3627 3939 5267 6651 6919 Size: 583 489 157 766 312 1328 1384 268 Cuts at: 6919 1632 Size: 1917 Fragments arranged by size: 1917 1384 1328 766 583 489 312 268 157 AflII C'TTAA_G Cuts at: 820 1017 4232 820 Size: 197 3215 3792 Fragments arranged by size: 3792 3215 197 AflIII A'CryG_T Cuts at: 1203 2973 6103 7080 1203 Size: 1770 3130 977 1327 Fragments arranged by size: 3130 1770 1327 977 AgeI A'CCGG_T Cuts at: 6035 6035 Size: 7204 AhdI GACnn_n'nnGTC Cuts at: 5933 5933 Size: 7204 AleI CACnn'nnGTG Cuts at: 1166 3704 1166 Size: 2538 4666 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3467 1184 Size: 2283 4921 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2559 2591 2559 Size: 32 7172 AlwNI CAG_nnn'CTG Cuts at: 3579 3838 4312 4471 4947 6519 3579 Size: 259 474 159 476 1572 4264 Fragments arranged by size: 4264 1572 476 474 259 159 ApaI G_GGCC'C Cuts at: 2595 3035 3372 3976 4278 2595 Size: 440 337 604 302 5521 Fragments arranged by size: 5521 604 440 337 302 ApaLI G'TGCA_C Cuts at: 2413 5261 6417 2413 Size: 2848 1156 3200 Fragments arranged by size: 3200 2848 1156 ApoI r'AATT_y Cuts at: 1493 4513 4739 4891 7000 1493 Size: 3020 226 152 2109 1697 Fragments arranged by size: 3020 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5758 160 Size: 872 4726 1606 Fragments arranged by size: 4726 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7204 AvaI C'yCGr_G Cuts at: 1870 1943 2211 3540 3803 3969 4409 4487 4529 Size: 73 268 1329 263 166 440 78 42 Cuts at: 4529 1870 Size: 4545 Fragments arranged by size: 4545 1329 440 268 263 166 78 73 42 BamHI G'GATC_C Cuts at: 4198 4534 4901 4198 Size: 336 367 6501 Fragments arranged by size: 6501 367 336 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2207 2390 3132 3254 4257 Size: 325 217 627 420 183 742 122 1003 Cuts at: 4257 4413 4525 5980 618 Size: 156 112 1455 1842 Fragments arranged by size: 1842 1455 1003 742 627 420 325 217 183 156 122 112 BanII G_rGCy'C Cuts at: 1391 2187 2595 3035 3372 3417 3976 4149 4278 Size: 796 408 440 337 45 559 173 129 Cuts at: 4278 4410 4431 4523 1391 Size: 132 21 92 4072 Fragments arranged by size: 4072 796 559 440 408 337 173 132 129 92 45 21 BbeI G_GCGC'C Cuts at: 2211 3136 2211 Size: 925 6279 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2097 2297 2615 928 Size: 971 198 200 318 5517 Fragments arranged by size: 5517 971 318 200 198 BbvCI CC'TCA_GC Cuts at: 2067 2067 Size: 7204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2732 2766 3933 3967 5395 5429 1903 Size: 34 795 34 1167 34 1428 34 3678 Fragments arranged by size: 3678 1428 1167 795 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2312 5096 6306 2312 Size: 2784 1210 3210 Fragments arranged by size: 3210 2784 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7204 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2191 2216 2508 Size: 107 122 71 631 605 519 25 292 Cuts at: 2508 4022 5815 136 Size: 1514 1793 1525 Fragments arranged by size: 1793 1525 1514 631 605 519 292 122 107 71 25 BglII A'GATC_T Cuts at: 7199 7199 Size: 7204 BlpI GC'TnA_GC Cuts at: 4621 4621 Size: 7204 Bme1580I G_kGCm'C Cuts at: 1790 2417 2564 2595 3035 3257 3372 3716 3976 Size: 627 147 31 440 222 115 344 260 Cuts at: 3976 4278 4416 5265 6421 1790 Size: 302 138 849 1156 2573 Fragments arranged by size: 2573 1156 849 627 440 344 302 260 222 147 138 115 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7204 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2026 2199 4935 Size: 416 53 83 399 24 609 173 2736 Cuts at: 4935 5893 442 Size: 958 1753 Fragments arranged by size: 2736 1753 958 609 416 399 173 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7204 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2551 2551 Size: 7204 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 3176 3580 3815 4106 4151 4275 5864 Size: 1028 909 404 235 291 45 124 1589 Cuts at: 5864 1239 Size: 2579 Fragments arranged by size: 2579 1589 1028 909 404 291 235 124 45 Bpu10I CC'TnA_GC Cuts at: 2067 2496 4425 2067 Size: 429 1929 4846 Fragments arranged by size: 4846 1929 429 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3335 5329 6194 6492 3335 Size: 1994 865 298 4047 Fragments arranged by size: 4047 1994 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5867 882 Size: 617 4368 2219 Fragments arranged by size: 4368 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2203 4372 4995 493 Size: 711 999 2169 623 2702 Fragments arranged by size: 2702 2169 999 711 623 BsaBI GATnn'nnATC Cuts at: 7198 7198 Size: 7204 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2208 3133 5011 5393 Size: 53 83 186 936 675 925 1878 382 Cuts at: 5393 6028 275 Size: 635 1451 Fragments arranged by size: 1878 1451 936 925 675 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5637 6035 6309 6456 6867 5637 Size: 398 274 147 411 5974 Fragments arranged by size: 5974 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2354 2384 3134 3164 3728 Size: 30 165 30 951 30 750 30 564 Cuts at: 3728 3758 1178 Size: 30 4624 Fragments arranged by size: 4624 951 750 564 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3221 3404 3733 3908 4505 1186 Size: 2035 183 329 175 597 3885 Fragments arranged by size: 3885 2035 597 329 183 175 BseYI C'CCAG_C Cuts at: 2491 3092 3855 6407 6903 2491 Size: 601 763 2552 496 2792 Fragments arranged by size: 2792 2552 763 601 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3503 3543 1163 Size: 573 1767 40 4824 Fragments arranged by size: 4824 1767 573 40 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 4654 5415 6443 1540 Size: 275 186 1282 1371 761 1028 2301 Fragments arranged by size: 2301 1371 1282 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2417 4431 5265 5350 6421 2417 Size: 2014 834 85 1071 3200 Fragments arranged by size: 3200 2014 1071 834 85 BsmI GAATG_Cn' Cuts at: 4715 4808 4715 Size: 93 7111 BsmBI CGTCTCn'nnnn_ Cuts at: 3330 4438 3330 Size: 1108 6096 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2417 2564 2595 3035 3257 3372 Size: 399 397 230 147 31 440 222 115 Cuts at: 3372 3417 3716 3976 4149 4278 4410 4416 4431 Size: 45 299 260 173 129 132 6 15 Cuts at: 4431 4523 5265 5350 6421 1391 Size: 92 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 440 399 397 299 260 230 222 173 147 132 129 115 92 85 45 31 15 6 BspEI T'CCGG_A Cuts at: 6867 6867 Size: 7204 BspHI T'CATG_A Cuts at: 5093 6823 5093 Size: 1730 5474 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2749 3202 3795 4559 844 Size: 1905 453 593 764 3489 Fragments arranged by size: 3489 1905 764 593 453 BsrBI CCG'CTC Cuts at: 1394 1823 2728 3471 5091 1394 Size: 429 905 743 1620 3507 Fragments arranged by size: 3507 1620 905 743 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4058 5699 5873 65 Size: 1183 2810 1641 174 1396 Fragments arranged by size: 2810 1641 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2147 3461 4065 5848 6035 1700 Size: 110 337 1314 604 1783 187 2869 Fragments arranged by size: 2869 1783 1314 604 337 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7204 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7204 BssSI C'ACGA_G Cuts at: 2769 3109 3242 5264 6276 2769 Size: 340 133 2022 1012 3697 Fragments arranged by size: 3697 2022 1012 340 133 BstAPI GCAn_nnn'nTGC Cuts at: 794 2861 2902 2911 4420 794 Size: 2067 41 9 1509 3578 Fragments arranged by size: 3578 2067 1509 41 9 BstBI TT'CG_AA Cuts at: 6008 6008 Size: 7204 BstEII G'GTnAC_C Cuts at: 1184 3955 1184 Size: 2771 4433 BstXI CCAn_nnnn'nTGG Cuts at: 3154 4268 3154 Size: 1114 6090 BstYI r'GATC_y Cuts at: 1972 3012 3605 4198 4534 4901 5286 5303 6755 Size: 1040 593 593 336 367 385 17 1452 Cuts at: 6755 6841 6853 7199 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1040 593 593 385 367 346 336 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2121 2280 3136 3492 3588 3661 Size: 552 939 117 159 856 356 96 73 Cuts at: 3661 513 Size: 4056 Fragments arranged by size: 4056 939 856 552 356 159 117 96 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2915 487 Size: 683 56 1689 4776 Fragments arranged by size: 4776 1689 683 56 BtsI GCAGTG_nn' Cuts at: 2739 3880 4469 4720 5514 5534 2739 Size: 1141 589 251 794 20 4409 Fragments arranged by size: 4409 1141 794 589 251 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7204 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2926 2945 2961 2980 3643 3678 529 Size: 35 2362 19 16 19 663 35 4055 Fragments arranged by size: 4055 2362 663 35 35 19 19 16 DraI TTT'AAA Cuts at: 4508 4508 Size: 7204 DraIII CAC_nnn'GTG Cuts at: 3274 3274 Size: 7204 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2705 6211 809 Size: 507 1389 3506 1802 Fragments arranged by size: 3506 1802 1389 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 2538 2985 Size: 54 1425 50 275 18 288 420 447 Cuts at: 2985 3276 3495 3585 3595 4651 5540 7169 8 Size: 291 219 90 10 1056 889 1629 43 Fragments arranged by size: 1629 1425 1056 889 447 420 291 288 275 219 90 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 4651 1537 Size: 275 2839 4090 Fragments arranged by size: 4090 2839 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3003 4074 5134 1353 Size: 1650 1071 1060 3423 Fragments arranged by size: 3423 1650 1071 1060 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4363 5791 6165 6311 387 Size: 1064 138 2774 1428 374 146 1280 Fragments arranged by size: 2774 1428 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4429 4429 Size: 7204 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2215 2267 2704 2861 3176 3580 3627 Size: 393 583 52 437 157 315 404 47 Cuts at: 3627 3815 3939 4106 4151 4275 5267 5864 6651 Size: 188 124 167 45 124 992 597 787 Cuts at: 6651 6919 1239 Size: 268 1524 Fragments arranged by size: 1524 992 787 597 583 437 404 393 315 268 188 167 157 124 124 52 47 45 EcoNI CCTnn'n_nnAGG Cuts at: 3386 3386 Size: 7204 EcoO109I rG'GnC_Cy Cuts at: 2050 2218 2242 2275 2591 2592 3031 3032 3218 Size: 168 24 33 316 1 439 1 186 Cuts at: 3218 3368 3369 3544 3784 3807 3835 3873 3973 Size: 150 1 175 240 23 28 38 100 Cuts at: 3973 4113 4274 4275 2050 Size: 140 161 1 4979 Fragments arranged by size: 4979 439 316 240 186 175 168 161 150 140 100 38 33 28 24 23 1 1 1 1 EcoRI G'AATT_C Cuts at: 4513 4891 4513 Size: 378 6826 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2728 3472 3944 2728 Size: 744 472 5988 Fragments arranged by size: 5988 744 472 FspI TGC'GCA Cuts at: 5710 5710 Size: 7204 HaeII r_GCGC'y Cuts at: 1678 2211 3136 6351 1678 Size: 533 925 3215 2531 Fragments arranged by size: 3215 2531 925 533 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4481 4513 5845 5877 5919 Size: 32 135 32 2909 32 1332 32 42 Cuts at: 5919 5951 1373 Size: 32 2626 Fragments arranged by size: 2909 2626 1332 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2425 3568 4548 4794 669 Size: 988 281 487 1143 980 246 3079 Fragments arranged by size: 3079 1143 988 980 487 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7204 HpaI GTT'AAC Cuts at: 4794 4794 Size: 7204 KasI G'GCGC_C Cuts at: 2207 3132 2207 Size: 925 6279 KpnI G_GTAC'C Cuts at: 4529 4529 Size: 7204 MfeI C'AATT_G Cuts at: 4803 4803 Size: 7204 MluI A'CGCG_T Cuts at: 7080 7080 Size: 7204 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2766 2985 3771 4346 4506 6318 6502 1305 Size: 1461 219 786 575 160 1812 184 2007 Fragments arranged by size: 2007 1812 1461 786 575 219 184 160 MscI TGG'CCA Cuts at: 10 64 1489 1832 2120 3597 10 Size: 54 1425 343 288 1477 3617 Fragments arranged by size: 3617 1477 1425 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 3701 3704 5162 5521 5680 518 Size: 648 2535 3 1458 359 159 2042 Fragments arranged by size: 2535 2042 1458 648 359 159 3 MspA1I CmG'CkG Cuts at: 1606 2893 3019 3199 3400 3478 3487 3579 3765 Size: 1287 126 180 201 78 9 92 186 Cuts at: 3765 4620 5297 6445 6690 1606 Size: 855 677 1148 245 2120 Fragments arranged by size: 2120 1287 1148 855 677 245 201 186 180 126 92 78 9 NaeI GCC'GGC Cuts at: 3463 4067 3463 Size: 604 6600 NarI GG'CG_CC Cuts at: 2208 3133 2208 Size: 925 6279 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5713 Fragments arranged by size: 5713 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7204 NgoMIV G'CCGG_C Cuts at: 3461 4065 3461 Size: 604 6600 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7204 NotI GC'GGCC_GC Cuts at: 4651 4651 Size: 7204 NspI r_CATG'y Cuts at: 1132 1229 2431 2859 2977 3626 4253 4562 6107 Size: 97 1202 428 118 649 627 309 1545 Cuts at: 6107 1132 Size: 2229 Fragments arranged by size: 2229 1545 1202 649 627 428 309 118 97 PasI CC'CwG_GG Cuts at: 1348 3372 4018 1348 Size: 2024 646 4534 Fragments arranged by size: 4534 2024 646 PciI A'CATG_T Cuts at: 2973 6103 2973 Size: 3130 4074 PflMI CCAn_nnn'nTGG Cuts at: 1108 3274 3838 1108 Size: 2166 564 4474 Fragments arranged by size: 4474 2166 564 PfoI T'CCnGG_A Cuts at: 2154 2154 Size: 7204 PmeI GTTT'AAAC Cuts at: 4508 4508 Size: 7204 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5273 5305 6786 6818 5273 Size: 32 1481 32 5659 Fragments arranged by size: 5659 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3544 3784 3807 4113 3544 Size: 240 23 306 6635 Fragments arranged by size: 6635 306 240 23 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7204 PsiI TTA'TAA Cuts at: 4774 4774 Size: 7204 PspOMI G'GGCC_C Cuts at: 2591 3031 3368 3972 4274 2591 Size: 440 337 604 302 5521 Fragments arranged by size: 5521 604 440 337 302 PstI C_TGCA'G Cuts at: 830 2358 2820 2898 2916 3399 4458 4556 830 Size: 1528 462 78 18 483 1059 98 3478 Fragments arranged by size: 3478 1528 1059 483 462 98 78 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7204 PvuII CAG'CTG Cuts at: 2893 3400 3478 3487 2893 Size: 507 78 9 6610 Fragments arranged by size: 6610 507 78 9 SacI G_AGCT'C Cuts at: 4431 4431 Size: 7204 SalI G'TCGA_C Cuts at: 1936 4546 1936 Size: 2610 4594 SanDI GG'GwC_CC Cuts at: 3544 3544 Size: 7204 SapI GCTCTTCn'nnn_ Cuts at: 4074 4074 Size: 7204 SbfI CC_TGCA'GG Cuts at: 2916 4556 2916 Size: 1640 5564 ScaI AGT'ACT Cuts at: 5452 7164 5452 Size: 1712 5492 SexAI A'CCwGG_T Cuts at: 3270 3720 3270 Size: 450 6754 SfcI C'TryA_G Cuts at: 826 1045 2354 2816 2894 2912 3395 4454 4552 Size: 219 1309 462 78 18 483 1059 98 Cuts at: 4552 5687 6368 6559 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1309 1135 1059 681 483 462 219 191 98 78 18 SfiI GGCCn_nnn'nGGCC Cuts at: 2508 2508 Size: 7204 SfoI GGC'GCC Cuts at: 2209 3134 2209 Size: 925 6279 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7204 SgrAI Cr'CCGG_yG Cuts at: 1700 4065 1700 Size: 2365 4839 SmaI CCC'GGG Cuts at: 1872 3542 3805 3971 4531 1872 Size: 1670 263 166 560 4545 Fragments arranged by size: 4545 1670 560 263 166 SmlI C'TyrA_G Cuts at: 820 1017 1943 3350 4232 5308 6209 6471 820 Size: 197 926 1407 882 1076 901 262 1553 Fragments arranged by size: 1553 1407 1076 926 901 882 262 197 SnaBI TAC'GTA Cuts at: 493 4995 493 Size: 4502 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7204 SphI G_CATG'C Cuts at: 1132 2859 4253 4562 1132 Size: 1727 1394 309 3774 Fragments arranged by size: 3774 1727 1394 309 SrfI GCCC'GGGC Cuts at: 3971 3971 Size: 7204 SspI AAT'ATT Cuts at: 5 52 5128 5 Size: 47 5076 2081 Fragments arranged by size: 5076 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7204 StyI C'CwwG_G Cuts at: 513 1065 2004 3960 4098 4116 4140 4643 513 Size: 552 939 1956 138 18 24 503 3074 Fragments arranged by size: 3074 1956 939 552 503 138 24 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2342 3281 3581 5231 5248 5401 5586 Size: 1255 762 939 300 1650 17 153 185 Cuts at: 5586 6031 325 Size: 445 1498 Fragments arranged by size: 1650 1498 1255 939 762 445 300 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2532 5450 7162 Size: 274 80 33 51 1443 555 2918 1712 Cuts at: 7162 96 Size: 138 Fragments arranged by size: 2918 1712 1443 555 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3728 3760 7164 7196 3728 Size: 32 3404 32 3736 Fragments arranged by size: 3736 3404 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2101 2705 3530 1316 Size: 785 604 825 4990 Fragments arranged by size: 4990 825 785 604 XbaI T'CTAG_A Cuts at: 4540 4540 Size: 7204 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3424 3580 3424 Size: 156 7048 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7204 XmaI C'CCGG_G Cuts at: 1870 3540 3803 3969 4529 1870 Size: 1670 263 166 560 4545 Fragments arranged by size: 4545 1670 560 263 166 XmnI GAAnn'nnTTC Cuts at: 5333 5333 Size: 7204 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5012 276 Size: 53 83 186 936 3478 2468 Fragments arranged by size: 3478 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SwaI