(Circular) (Six-base) MAPSORT of: pFN21AA1726.seq Check: 3284 from: 1 to: 7357 pFN21AA1726 7357 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4143 4143 Size: 7357 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5167 278 Size: 53 83 186 936 3631 2468 Fragments arranged by size: 3631 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3504 3762 4700 1937 Size: 1567 258 938 4594 Fragments arranged by size: 4594 1567 938 258 Acc65I G'GTAC_C Cuts at: 4371 4678 4371 Size: 307 7050 AclI AA'CG_TT Cuts at: 1563 5484 5857 1563 Size: 3921 373 3063 Fragments arranged by size: 3921 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2271 2298 2454 2553 5420 6804 7072 1632 Size: 639 27 156 99 2867 1384 268 1917 Fragments arranged by size: 2867 1917 1384 639 268 156 99 27 AflII C'TTAA_G Cuts at: 820 1017 2516 820 Size: 197 1499 5661 Fragments arranged by size: 5661 1499 197 AflIII A'CryG_T Cuts at: 1203 2087 6256 7233 1203 Size: 884 4169 977 1327 Fragments arranged by size: 4169 1327 977 884 AgeI A'CCGG_T Cuts at: 3942 6188 3942 Size: 2246 5111 AhdI GACnn_n'nnGTC Cuts at: 6086 6086 Size: 7357 AleI CACnn'nnGTG Cuts at: 1166 4411 1166 Size: 3245 4112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7357 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4080 4112 4080 Size: 32 7325 AlwNI CAG_nnn'CTG Cuts at: 3338 5100 6672 3338 Size: 1762 1572 4023 Fragments arranged by size: 4023 1762 1572 ApaLI G'TGCA_C Cuts at: 5414 6570 5414 Size: 1156 6201 ApoI r'AATT_y Cuts at: 1493 2290 2412 2928 3982 4042 4666 4892 5044 Size: 797 122 516 1054 60 624 226 152 Cuts at: 5044 7153 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1054 797 624 516 226 152 122 60 AseI AT'TA_AT Cuts at: 160 1032 3131 5911 160 Size: 872 2099 2780 1606 Fragments arranged by size: 2780 2099 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7357 AvaI C'yCGr_G Cuts at: 1870 1943 4501 4682 1870 Size: 73 2558 181 4545 Fragments arranged by size: 4545 2558 181 73 AvrII C'CTAG_G Cuts at: 2119 4222 2119 Size: 2103 5254 BamHI G'GATC_C Cuts at: 4470 4687 5054 4470 Size: 217 367 6773 Fragments arranged by size: 6773 367 217 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4154 4213 4371 4678 6133 Size: 325 217 627 2367 59 158 307 1455 Cuts at: 6133 618 Size: 1842 Fragments arranged by size: 2367 1842 1455 627 325 307 217 158 59 BanII G_rGCy'C Cuts at: 1391 4676 1391 Size: 3285 4072 BbeI G_GCGC'C Cuts at: 4158 4158 Size: 7357 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6386 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5548 5582 1903 Size: 34 3611 34 3678 Fragments arranged by size: 3678 3611 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2206 3750 5249 6459 2206 Size: 1544 1499 1210 3104 Fragments arranged by size: 3104 1544 1499 1210 BclI T'GATC_A Cuts at: 1548 2296 2632 1548 Size: 748 336 6273 Fragments arranged by size: 6273 748 336 BfrBI ATG'CAT Cuts at: 3791 3791 Size: 7357 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2977 4323 4465 Size: 107 122 71 631 605 1305 1346 142 Cuts at: 4465 5968 136 Size: 1503 1525 Fragments arranged by size: 1525 1503 1346 1305 631 605 142 122 107 71 BglII A'GATC_T Cuts at: 7352 7352 Size: 7357 BlpI GC'TnA_GC Cuts at: 4774 4774 Size: 7357 Bme1580I G_kGCm'C Cuts at: 1790 5418 6574 1790 Size: 3628 1156 2573 Fragments arranged by size: 3628 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7357 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3189 5088 6046 Size: 416 53 83 399 24 1772 1899 958 Cuts at: 6046 442 Size: 1753 Fragments arranged by size: 1899 1772 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7357 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2525 3660 6017 1239 Size: 1286 1135 2357 2579 Fragments arranged by size: 2579 2357 1286 1135 Bpu10I CC'TnA_GC Cuts at: 4446 4446 Size: 7357 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2384 5482 6347 6645 2384 Size: 3098 865 298 3096 Fragments arranged by size: 3098 3096 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2379 2737 6020 882 Size: 617 880 358 3283 2219 Fragments arranged by size: 3283 2219 880 617 358 BsaAI yAC'GTr Cuts at: 493 1204 2090 2936 3928 5148 493 Size: 711 886 846 992 1220 2702 Fragments arranged by size: 2702 1220 992 886 846 711 BsaBI GATnn'nnATC Cuts at: 4532 7351 4532 Size: 2819 4538 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2246 4155 4362 4605 Size: 53 83 186 936 713 1909 207 243 Cuts at: 4605 5164 5546 6181 275 Size: 559 382 635 1451 Fragments arranged by size: 1909 1451 936 713 635 559 382 243 207 186 83 53 BsaWI w'CCGG_w Cuts at: 3942 5790 6188 6462 6609 7020 3942 Size: 1848 398 274 147 411 4279 Fragments arranged by size: 4279 1848 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2589 2619 3384 3414 1178 Size: 30 165 30 1186 30 765 30 5121 Fragments arranged by size: 5121 1186 765 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3007 3992 4101 4179 1186 Size: 1821 985 109 78 4364 Fragments arranged by size: 4364 1821 985 109 78 BseYI C'CCAG_C Cuts at: 3580 4335 4608 4644 4648 6560 7056 3580 Size: 755 273 36 4 1912 496 3881 Fragments arranged by size: 3881 1912 755 496 273 36 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4115 1163 Size: 573 2379 4405 Fragments arranged by size: 4405 2379 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4807 5568 6596 1540 Size: 275 186 2806 761 1028 2301 Fragments arranged by size: 2806 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2156 3466 5418 5503 6574 2156 Size: 1310 1952 85 1071 2939 Fragments arranged by size: 2939 1952 1310 1071 85 BsmI GAATG_Cn' Cuts at: 2028 2046 2263 3793 4491 4868 4961 2028 Size: 18 217 1530 698 377 93 4424 Fragments arranged by size: 4424 1530 698 377 217 93 18 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2156 3466 4676 5418 5503 6574 1391 Size: 399 366 1310 1210 742 85 1071 2174 Fragments arranged by size: 2174 1310 1210 1071 742 399 366 85 BspEI T'CCGG_A Cuts at: 7020 7020 Size: 7357 BspHI T'CATG_A Cuts at: 2299 4578 5246 6976 2299 Size: 2279 668 1730 2680 Fragments arranged by size: 2680 2279 1730 668 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2361 4143 4712 844 Size: 1517 1782 569 3489 Fragments arranged by size: 3489 1782 1517 569 BsrBI CCG'CTC Cuts at: 1394 1823 5244 1394 Size: 429 3421 3507 Fragments arranged by size: 3507 3421 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2413 2773 2781 3631 5852 6026 65 Size: 1183 1165 360 8 850 2221 174 1396 Fragments arranged by size: 2221 1396 1183 1165 850 360 174 8 BsrFI r'CCGG_y Cuts at: 1700 1810 3942 4374 6001 6188 1700 Size: 110 2132 432 1627 187 2869 Fragments arranged by size: 2869 2132 1627 432 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7357 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7357 BssSI C'ACGA_G Cuts at: 4243 5417 6429 4243 Size: 1174 1012 5171 Fragments arranged by size: 5171 1174 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7357 BstBI TT'CG_AA Cuts at: 6161 6161 Size: 7357 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7357 BstXI CCAn_nnnn'nTGG Cuts at: 4633 4633 Size: 7357 BstYI r'GATC_y Cuts at: 1972 3481 4470 4495 4687 5054 5439 5456 6908 Size: 1509 989 25 192 367 385 17 1452 Cuts at: 6908 6994 7006 7352 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1509 1452 989 385 367 346 192 86 25 17 12 Bsu36I CC'TnA_GG Cuts at: 3657 3657 Size: 7357 BtgI C'CryG_G Cuts at: 513 1065 2004 2782 513 Size: 552 939 778 5088 Fragments arranged by size: 5088 939 778 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6618 Fragments arranged by size: 6618 683 56 BtsI GCAGTG_nn' Cuts at: 2832 3895 3922 4873 5667 5687 2832 Size: 1063 27 951 794 20 4502 Fragments arranged by size: 4502 1063 951 794 27 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7357 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7322 DraI TTT'AAA Cuts at: 2428 4661 2428 Size: 2233 5124 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3758 6364 809 Size: 507 2442 2606 1802 Fragments arranged by size: 2606 2442 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2134 4239 4804 Size: 54 1425 50 275 18 304 2105 565 Cuts at: 4804 5693 7322 8 Size: 889 1629 43 Fragments arranged by size: 2105 1629 1425 889 565 304 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4804 1537 Size: 275 2992 4090 Fragments arranged by size: 4090 2992 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2012 2247 2430 4119 5287 1353 Size: 659 235 183 1689 1168 3423 Fragments arranged by size: 3423 1689 1168 659 235 183 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4483 5944 6318 6464 387 Size: 1064 138 2894 1461 374 146 1280 Fragments arranged by size: 2894 1461 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2271 2298 2454 2525 2553 3660 5420 Size: 393 639 27 156 71 28 1107 1760 Cuts at: 5420 6017 6804 7072 1239 Size: 597 787 268 1524 Fragments arranged by size: 1760 1524 1107 787 639 597 393 268 156 71 28 27 EcoO109I rG'GnC_Cy Cuts at: 4230 4230 Size: 7357 EcoRI G'AATT_C Cuts at: 4666 5044 4666 Size: 378 6979 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3542 4323 3542 Size: 781 6576 FspI TGC'GCA Cuts at: 5863 5863 Size: 7357 HaeII r_GCGC'y Cuts at: 1678 4158 6504 1678 Size: 2480 2346 2531 Fragments arranged by size: 2531 2480 2346 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2288 2320 2589 2621 4169 Size: 32 135 32 716 32 269 32 1548 Cuts at: 4169 4201 4244 4276 5998 6030 6072 6104 1373 Size: 32 43 32 1722 32 42 32 2626 Fragments arranged by size: 2626 1722 1548 716 269 135 43 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2190 3763 3995 4701 4947 669 Size: 988 281 252 1573 232 706 246 3079 Fragments arranged by size: 3079 1573 988 706 281 252 246 232 HindIII A'AGCT_T Cuts at: 748 3524 748 Size: 2776 4581 HpaI GTT'AAC Cuts at: 4947 4947 Size: 7357 KasI G'GCGC_C Cuts at: 4154 4154 Size: 7357 KpnI G_GTAC'C Cuts at: 4375 4682 4375 Size: 307 7050 MfeI C'AATT_G Cuts at: 2312 4956 2312 Size: 2644 4713 MluI A'CGCG_T Cuts at: 7233 7233 Size: 7357 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3024 6471 6655 1305 Size: 1719 3447 184 2007 Fragments arranged by size: 3447 2007 1719 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4241 10 Size: 54 1425 343 2409 3126 Fragments arranged by size: 3126 2409 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2142 4411 4631 5315 5674 5833 518 Size: 648 976 2269 220 684 359 159 2042 Fragments arranged by size: 2269 2042 976 684 648 359 220 159 MspA1I CmG'CkG Cuts at: 1606 2177 3328 4148 4167 4648 4773 5450 6598 Size: 571 1151 820 19 481 125 677 1148 Cuts at: 6598 6843 1606 Size: 245 2120 Fragments arranged by size: 2120 1151 1148 820 677 571 481 245 125 19 NarI GG'CG_CC Cuts at: 4155 4155 Size: 7357 NcoI C'CATG_G Cuts at: 513 1065 2004 2782 513 Size: 552 939 778 5088 Fragments arranged by size: 5088 939 778 552 NdeI CA'TA_TG Cuts at: 387 3259 387 Size: 2872 4485 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7357 NotI GC'GGCC_GC Cuts at: 4804 4804 Size: 7357 NsiI A_TGCA'T Cuts at: 3793 3793 Size: 7357 NspI r_CATG'y Cuts at: 1132 1229 4003 4715 6260 1132 Size: 97 2774 712 1545 2229 Fragments arranged by size: 2774 2229 1545 712 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7357 PciI A'CATG_T Cuts at: 6256 6256 Size: 7357 PflMI CCAn_nnn'nTGG Cuts at: 1108 4352 4481 1108 Size: 3244 129 3984 Fragments arranged by size: 3984 3244 129 PmeI GTTT'AAAC Cuts at: 4661 4661 Size: 7357 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5426 5458 6939 6971 5426 Size: 32 1481 32 5812 Fragments arranged by size: 5812 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4230 4230 Size: 7357 PshAI GACnn'nnGTC Cuts at: 1613 3500 1613 Size: 1887 5470 PsiI TTA'TAA Cuts at: 4927 4927 Size: 7357 PstI C_TGCA'G Cuts at: 830 2372 2810 3887 4041 4709 830 Size: 1542 438 1077 154 668 3478 Fragments arranged by size: 3478 1542 1077 668 438 154 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7357 PvuII CAG'CTG Cuts at: 2177 4167 4648 2177 Size: 1990 481 4886 Fragments arranged by size: 4886 1990 481 SalI G'TCGA_C Cuts at: 1936 3761 4699 1936 Size: 1825 938 4594 Fragments arranged by size: 4594 1825 938 SbfI CC_TGCA'GG Cuts at: 4709 4709 Size: 7357 ScaI AGT'ACT Cuts at: 5605 7317 5605 Size: 1712 5645 SexAI A'CCwGG_T Cuts at: 3551 3551 Size: 7357 SfcI C'TryA_G Cuts at: 826 1045 2368 2806 3073 3883 4037 4705 5840 Size: 219 1323 438 267 810 154 668 1135 Cuts at: 5840 6521 6712 826 Size: 681 191 1471 Fragments arranged by size: 1471 1323 1135 810 681 668 438 267 219 191 154 SfoI GGC'GCC Cuts at: 4156 4156 Size: 7357 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7357 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7357 SmaI CCC'GGG Cuts at: 1872 4684 1872 Size: 2812 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2399 2516 5461 6362 6624 820 Size: 197 926 456 117 2945 901 262 1553 Fragments arranged by size: 2945 1553 926 901 456 262 197 117 SnaBI TAC'GTA Cuts at: 493 2936 5148 493 Size: 2443 2212 2702 Fragments arranged by size: 2702 2443 2212 SpeI A'CTAG_T Cuts at: 152 152 Size: 7357 SphI G_CATG'C Cuts at: 1132 4715 1132 Size: 3583 3774 SspI AAT'ATT Cuts at: 5 52 3296 5281 5 Size: 47 3244 1985 2081 Fragments arranged by size: 3244 2081 1985 47 StuI AGG'CCT Cuts at: 1517 4326 1517 Size: 2809 4548 StyI C'CwwG_G Cuts at: 513 1065 2004 2119 2183 2454 2782 3429 4222 Size: 552 939 115 64 271 328 647 793 Cuts at: 4222 4233 4796 513 Size: 11 563 3074 Fragments arranged by size: 3074 939 793 647 563 552 328 271 115 64 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5384 5401 5554 5739 6184 325 Size: 1255 3804 17 153 185 445 1498 Fragments arranged by size: 3804 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2084 2551 2645 Size: 274 80 33 51 1443 107 467 94 Cuts at: 2645 5603 7315 96 Size: 2958 1712 138 Fragments arranged by size: 2958 1712 1443 467 274 138 107 94 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3384 3416 7317 7349 3384 Size: 32 3901 32 3392 Fragments arranged by size: 3901 3392 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7357 XbaI T'CTAG_A Cuts at: 3359 4693 3359 Size: 1334 6023 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2181 3541 2181 Size: 1360 5997 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7357 XmaI C'CCGG_G Cuts at: 1870 4682 1870 Size: 2812 4545 XmnI GAAnn'nnTTC Cuts at: 2541 3110 3525 3865 5486 2541 Size: 569 415 340 1621 4412 Fragments arranged by size: 4412 1621 569 415 340 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5165 276 Size: 53 83 186 936 3631 2468 Fragments arranged by size: 3631 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbvCI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI