Restriction map for
pFN21AA1726
(Linear) (Six-base) MAPSORT of: KIAA1726.seq Check: 3515 from: 1 to: 2652
KIAA1726.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2138 2652
Size: 2138 514
AccI GT'mk_AC
Cuts at: 0 1499 1757 2652
Size: 1499 258 895
Fragments arranged by size:
1499 895 258
Acc65I G'GTAC_C
Cuts at: 0 2366 2652
Size: 2366 286
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 293 449 548 2652
Size: 266 27 156 99 2104
Fragments arranged by size:
2104 266 156 99 27
AflII C'TTAA_G
Cuts at: 0 511 2652
Size: 511 2141
AflIII A'CryG_T
Cuts at: 0 82 2652
Size: 82 2570
AgeI A'CCGG_T
Cuts at: 0 1937 2652
Size: 1937 715
AleI CACnn'nnGTG
Cuts at: 0 2406 2652
Size: 2406 246
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2075 2107 2652
Size: 2075 32 545
Fragments arranged by size:
2075 545 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1333 2652
Size: 1333 1319
ApoI r'AATT_y
Cuts at: 0 285 407 923 1977 2037 2652
Size: 285 122 516 1054 60 615
Fragments arranged by size:
1054 615 516 285 122 60
AseI AT'TA_AT
Cuts at: 0 1126 2652
Size: 1126 1526
AvaI C'yCGr_G
Cuts at: 0 2496 2652
Size: 2496 156
AvrII C'CTAG_G
Cuts at: 0 114 2217 2652
Size: 114 2103 435
Fragments arranged by size:
2103 435 114
BamHI G'GATC_C
Cuts at: 0 2465 2652
Size: 2465 187
BanI G'GyrC_C
Cuts at: 0 2149 2208 2366 2652
Size: 2149 59 158 286
Fragments arranged by size:
2149 286 158 59
BbeI G_GCGC'C
Cuts at: 0 2153 2652
Size: 2153 499
BciVI GTATCCnnnnn_n'
Cuts at: 0 201 1745 2652
Size: 201 1544 907
Fragments arranged by size:
1544 907 201
BclI T'GATC_A
Cuts at: 0 291 627 2652
Size: 291 336 2025
Fragments arranged by size:
2025 336 291
BfrBI ATG'CAT
Cuts at: 0 1786 2652
Size: 1786 866
BglI GCCn_nnn'nGGC
Cuts at: 0 972 2318 2460 2652
Size: 972 1346 142 192
Fragments arranged by size:
1346 972 192 142
BmrI ACTGGGnnnn_n'
Cuts at: 0 1184 2652
Size: 1184 1468
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1655 2652
Size: 520 1135 997
Fragments arranged by size:
1135 997 520
Bpu10I CC'TnA_GC
Cuts at: 0 2441 2652
Size: 2441 211
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 2652
Size: 379 2273
BsaI GGTCTCn'nnnn_
Cuts at: 0 374 732 2652
Size: 374 358 1920
Fragments arranged by size:
1920 374 358
BsaAI yAC'GTr
Cuts at: 0 85 931 1923 2652
Size: 85 846 992 729
Fragments arranged by size:
992 846 729 85
BsaBI GATnn'nnATC
Cuts at: 0 2527 2652
Size: 2527 125
BsaHI Gr'CG_yC
Cuts at: 0 241 2150 2357 2600 2652
Size: 241 1909 207 243 52
Fragments arranged by size:
1909 243 241 207 52
BsaWI w'CCGG_w
Cuts at: 0 1937 2652
Size: 1937 715
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 584 614 1379 1409 2652
Size: 584 30 765 30 1243
Fragments arranged by size:
1243 765 584 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1002 1987 2096 2174 2652
Size: 1002 985 109 78 478
Fragments arranged by size:
1002 985 478 109 78
BseYI C'CCAG_C
Cuts at: 0 1575 2330 2603 2639 2643 2652
Size: 1575 755 273 36 4 9
Fragments arranged by size:
1575 755 273 36 9 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2110 2652
Size: 2110 542
BsiHKAI G_wGCw'C
Cuts at: 0 151 1461 2652
Size: 151 1310 1191
Fragments arranged by size:
1310 1191 151
BsmI GAATG_Cn'
Cuts at: 0 23 41 258 1788 2486 2652
Size: 23 18 217 1530 698 166
Fragments arranged by size:
1530 698 217 166 23 18
Bsp1286I G_dGCh'C
Cuts at: 0 151 1461 2652
Size: 151 1310 1191
Fragments arranged by size:
1310 1191 151
BspHI T'CATG_A
Cuts at: 0 294 2573 2652
Size: 294 2279 79
Fragments arranged by size:
2279 294 79
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 356 2138 2652
Size: 356 1782 514
Fragments arranged by size:
1782 514 356
BsrDI GCAATG_nn'
Cuts at: 0 408 768 776 1626 2652
Size: 408 360 8 850 1026
Fragments arranged by size:
1026 850 408 360 8
BsrFI r'CCGG_y
Cuts at: 0 1937 2369 2652
Size: 1937 432 283
Fragments arranged by size:
1937 432 283
BssSI C'ACGA_G
Cuts at: 0 2238 2652
Size: 2238 414
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2628 2652
Size: 2628 24
BstYI r'GATC_y
Cuts at: 0 1476 2465 2490 2652
Size: 1476 989 25 162
Fragments arranged by size:
1476 989 162 25
Bsu36I CC'TnA_GG
Cuts at: 0 1652 2652
Size: 1652 1000
BtgI C'CryG_G
Cuts at: 0 777 2652
Size: 777 1875
BtsI GCAGTG_nn'
Cuts at: 0 827 1890 1917 2652
Size: 827 1063 27 735
Fragments arranged by size:
1063 827 735 27
DraI TTT'AAA
Cuts at: 0 423 2652
Size: 423 2229
DrdI GACnn_nn'nnGTC
Cuts at: 0 1753 2652
Size: 1753 899
EaeI y'GGCC_r
Cuts at: 0 129 2234 2652
Size: 129 2105 418
Fragments arranged by size:
2105 418 129
EarI CTCTTCn'nnn_
Cuts at: 0 7 242 425 2114 2652
Size: 7 235 183 1689 538
Fragments arranged by size:
1689 538 235 183 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2478 2652
Size: 2478 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 293 449 520 548 1655 2652
Size: 266 27 156 71 28 1107 997
Fragments arranged by size:
1107 997 266 156 71 28 27
EcoO109I rG'GnC_Cy
Cuts at: 0 2225 2652
Size: 2225 427
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1537 2318 2652
Size: 1537 781 334
Fragments arranged by size:
1537 781 334
HaeII r_GCGC'y
Cuts at: 0 2153 2652
Size: 2153 499
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 584 616 2164 2196 2239 2271
Size: 283 32 269 32 1548 32 43 32
Cuts at: 2271 2652
Size: 381
Fragments arranged by size:
1548 381 283 269 43 32 32 32
32
HincII GTy'rAC
Cuts at: 0 185 1758 1990 2652
Size: 185 1573 232 662
Fragments arranged by size:
1573 662 232 185
HindIII A'AGCT_T
Cuts at: 0 1519 2652
Size: 1519 1133
KasI G'GCGC_C
Cuts at: 0 2149 2652
Size: 2149 503
KpnI G_GTAC'C
Cuts at: 0 2370 2652
Size: 2370 282
MfeI C'AATT_G
Cuts at: 0 307 2652
Size: 307 2345
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 2652
Size: 1019 1633
MscI TGG'CCA
Cuts at: 0 2236 2652
Size: 2236 416
MslI CAynn'nnrTG
Cuts at: 0 137 2406 2626 2652
Size: 137 2269 220 26
Fragments arranged by size:
2269 220 137 26
MspA1I CmG'CkG
Cuts at: 0 172 1323 2143 2162 2643 2652
Size: 172 1151 820 19 481 9
Fragments arranged by size:
1151 820 481 172 19 9
NarI GG'CG_CC
Cuts at: 0 2150 2652
Size: 2150 502
NcoI C'CATG_G
Cuts at: 0 777 2652
Size: 777 1875
NdeI CA'TA_TG
Cuts at: 0 1254 2652
Size: 1254 1398
NsiI A_TGCA'T
Cuts at: 0 1788 2652
Size: 1788 864
NspI r_CATG'y
Cuts at: 0 1998 2652
Size: 1998 654
PflMI CCAn_nnn'nTGG
Cuts at: 0 2347 2476 2652
Size: 2347 129 176
Fragments arranged by size:
2347 176 129
PpuMI rG'GwC_Cy
Cuts at: 0 2225 2652
Size: 2225 427
PshAI GACnn'nnGTC
Cuts at: 0 1495 2652
Size: 1495 1157
PstI C_TGCA'G
Cuts at: 0 367 805 1882 2036 2652
Size: 367 438 1077 154 616
Fragments arranged by size:
1077 616 438 367 154
PvuII CAG'CTG
Cuts at: 0 172 2162 2643 2652
Size: 172 1990 481 9
Fragments arranged by size:
1990 481 172 9
SalI G'TCGA_C
Cuts at: 0 1756 2652
Size: 1756 896
SexAI A'CCwGG_T
Cuts at: 0 1546 2652
Size: 1546 1106
SfcI C'TryA_G
Cuts at: 0 363 801 1068 1878 2032 2652
Size: 363 438 267 810 154 620
Fragments arranged by size:
810 620 438 363 267 154
SfoI GGC'GCC
Cuts at: 0 2151 2652
Size: 2151 501
SmlI C'TyrA_G
Cuts at: 0 394 511 2652
Size: 394 117 2141
Fragments arranged by size:
2141 394 117
SnaBI TAC'GTA
Cuts at: 0 931 2652
Size: 931 1721
SspI AAT'ATT
Cuts at: 0 1291 2652
Size: 1291 1361
StuI AGG'CCT
Cuts at: 0 2321 2652
Size: 2321 331
StyI C'CwwG_G
Cuts at: 0 114 178 449 777 1424 2217 2228 2652
Size: 114 64 271 328 647 793 11 424
Fragments arranged by size:
793 647 424 328 271 114 64 11
TatI w'GTAC_w
Cuts at: 0 79 546 640 2652
Size: 79 467 94 2012
Fragments arranged by size:
2012 467 94 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1379 1411 2652
Size: 1379 32 1241
Fragments arranged by size:
1379 1241 32
XbaI T'CTAG_A
Cuts at: 0 1354 2652
Size: 1354 1298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 176 1536 2652
Size: 176 1360 1116
Fragments arranged by size:
1360 1116 176
XmnI GAAnn'nnTTC
Cuts at: 0 536 1105 1520 1860 2652
Size: 536 569 415 340 792
Fragments arranged by size:
792 569 536 415 340
Enzymes that do cut:
AarI AccI Acc65I AcuI AflII AflIII AgeI AleI
AloI AlwNI ApoI AseI AvaI AvrII BamHI BanI
BbeI BciVI BclI BfrBI BglI BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BsrFI BssSI BstXI BstYI Bsu36I BtgI BtsI DraI
DrdI EaeI EarI EciI Eco57MI EcoO109I FalI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MmeI MscI
MslI MspA1I NarI NcoI NdeI NsiI NspI PflMI
PpuMI PshAI PstI PvuII SalI SexAI SfcI SfoI
SmlI SnaBI SspI StuI StyI TatI TstI XbaI
XcmI XmnI
Enzymes that do not cut:
AatII AclI AfeI AhdI AlfI ApaI ApaLI AscI
AsiSI BaeI BanII BbsI BbvCI BcgI BglII BlpI
Bme1580I BmgBI BmtI BplI BsiEI BsiWI BsmBI BspEI
BsrBI BsrGI BssHII BstAPI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DraIII EagI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HpaI MluI NaeI NgoMIV NheI
NotI NruI PacI PasI PciI PfoI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SmaI SpeI SphI SrfI SwaI TaqII Tth111I XhoI
XmaI ZraI