(Circular) (Six-base) MAPSORT of: pFN21AA1708.seq Check: 2859 from: 1 to: 9688 pFN21AA1708 9688 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3398 4546 3398 Size: 1148 8540 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7498 278 Size: 53 83 186 936 5962 2468 Fragments arranged by size: 5962 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5167 6416 7031 1937 Size: 3230 1249 615 4594 Fragments arranged by size: 4594 3230 1249 615 Acc65I G'GTAC_C Cuts at: 7009 7009 Size: 9688 AclI AA'CG_TT Cuts at: 1563 3077 7815 8188 1563 Size: 1514 4738 373 3063 Fragments arranged by size: 4738 3063 1514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2867 3039 3176 4467 5108 5361 5466 5745 Size: 1235 172 137 1291 641 253 105 279 Cuts at: 5745 5960 6015 6231 6677 7751 9135 9403 1632 Size: 215 55 216 446 1074 1384 268 1917 Fragments arranged by size: 1917 1384 1291 1235 1074 641 446 279 268 253 216 215 172 137 105 55 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9491 AflIII A'CryG_T Cuts at: 1203 2579 2962 3264 8587 9564 1203 Size: 1376 383 302 5323 977 1327 Fragments arranged by size: 5323 1376 1327 977 383 302 AgeI A'CCGG_T Cuts at: 8519 8519 Size: 9688 AhdI GACnn_n'nnGTC Cuts at: 6561 8417 6561 Size: 1856 7832 AleI CACnn'nnGTG Cuts at: 1166 5977 1166 Size: 4811 4877 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3180 1184 Size: 1996 7692 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4507 4539 4507 Size: 32 9656 AlwNI CAG_nnn'CTG Cuts at: 2837 3707 3869 3985 4568 5753 6771 7431 9003 Size: 870 162 116 583 1185 1018 660 1572 Cuts at: 9003 2837 Size: 3522 Fragments arranged by size: 3522 1572 1185 1018 870 660 583 162 116 ApaLI G'TGCA_C Cuts at: 7745 8901 7745 Size: 1156 8532 ApoI r'AATT_y Cuts at: 1493 2532 2556 2789 4029 4614 5667 6912 6997 Size: 1039 24 233 1240 585 1053 1245 85 Cuts at: 6997 7223 7375 9484 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1245 1240 1053 1039 585 233 226 152 85 24 AseI AT'TA_AT Cuts at: 160 1032 5052 8242 160 Size: 872 4020 3190 1606 Fragments arranged by size: 4020 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9688 AvaI C'yCGr_G Cuts at: 1870 1943 2349 3011 7013 1870 Size: 73 406 662 4002 4545 Fragments arranged by size: 4545 4002 662 406 73 AvrII C'CTAG_G Cuts at: 2131 6439 2131 Size: 4308 5380 BamHI G'GATC_C Cuts at: 7018 7385 7018 Size: 367 9321 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 6353 6742 7009 8464 Size: 325 217 627 225 4341 389 267 1455 Cuts at: 8464 618 Size: 1842 Fragments arranged by size: 4341 1842 1455 627 389 325 267 225 217 BanII G_rGCy'C Cuts at: 1391 2030 2457 2675 3204 4600 5598 6550 7007 Size: 639 427 218 529 1396 998 952 457 Cuts at: 7007 1391 Size: 4072 Fragments arranged by size: 4072 1396 998 952 639 529 457 427 218 BbeI G_GCGC'C Cuts at: 2016 2016 Size: 9688 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2119 5114 5367 6163 6237 928 Size: 971 220 2995 253 796 74 4379 Fragments arranged by size: 4379 2995 971 796 253 220 74 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4562 4596 7879 7913 1903 Size: 34 2625 34 3283 34 3678 Fragments arranged by size: 3678 3283 2625 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2234 4030 6799 7580 8790 2234 Size: 1796 2769 781 1210 3132 Fragments arranged by size: 3132 2769 1796 1210 781 BclI T'GATC_A Cuts at: 1548 3379 3790 1548 Size: 1831 411 7446 Fragments arranged by size: 7446 1831 411 BfrBI ATG'CAT Cuts at: 3347 4583 3347 Size: 1236 8452 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 8299 136 Size: 107 122 71 631 605 6627 1525 Fragments arranged by size: 6627 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2193 3466 5284 5380 6487 6945 6961 9683 2193 Size: 1273 1818 96 1107 458 16 2722 2198 Fragments arranged by size: 2722 2198 1818 1273 1107 458 96 16 BlpI GC'TnA_GC Cuts at: 7105 7105 Size: 9688 Bme1580I G_kGCm'C Cuts at: 1790 7749 8905 1790 Size: 5959 1156 2573 Fragments arranged by size: 5959 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9688 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2345 6102 6122 Size: 416 53 83 399 24 928 3757 20 Cuts at: 6122 6749 7419 8377 442 Size: 627 670 958 1753 Fragments arranged by size: 3757 1753 958 928 670 627 416 399 83 53 24 20 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9688 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2295 2406 2571 2877 5902 6333 8348 1239 Size: 1056 111 165 306 3025 431 2015 2579 Fragments arranged by size: 3025 2579 2015 1056 431 306 165 111 Bpu10I CC'TnA_GC Cuts at: 4542 5250 4542 Size: 708 8980 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4763 4802 7813 8678 8976 4763 Size: 39 3011 865 298 5475 Fragments arranged by size: 5475 3011 865 298 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4047 8351 882 Size: 617 2548 4304 2219 Fragments arranged by size: 4304 2548 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2582 7479 493 Size: 711 1378 4897 2702 Fragments arranged by size: 4897 2702 1378 711 BsaBI GATnn'nnATC Cuts at: 9682 9682 Size: 9688 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2013 7495 7877 8512 Size: 53 83 186 936 480 5482 382 635 Cuts at: 8512 275 Size: 1451 Fragments arranged by size: 5482 1451 936 635 480 382 186 83 53 BsaWI w'CCGG_w Cuts at: 8121 8519 8793 8940 9351 8121 Size: 398 274 147 411 8458 Fragments arranged by size: 8458 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2012 2042 2162 2192 3559 Size: 30 165 30 609 30 120 30 1367 Cuts at: 3559 3589 5900 5930 1178 Size: 30 2311 30 4936 Fragments arranged by size: 4936 2311 1367 609 165 120 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3437 3824 3827 3830 3833 3836 4805 5881 Size: 2251 387 3 3 3 3 969 1076 Cuts at: 5881 6032 1186 Size: 151 4842 Fragments arranged by size: 4842 2251 1076 969 387 151 3 3 3 3 BseYI C'CCAG_C Cuts at: 2181 4499 4599 8891 9387 2181 Size: 2318 100 4292 496 2482 Fragments arranged by size: 4292 2482 2318 496 100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4581 5044 1163 Size: 573 2845 463 5807 Fragments arranged by size: 5807 2845 573 463 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7138 7899 8927 1540 Size: 275 186 5137 761 1028 2301 Fragments arranged by size: 5137 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2030 2457 2675 3204 5598 6018 6550 7749 7834 Size: 427 218 529 2394 420 532 1199 85 Cuts at: 7834 8905 2030 Size: 1071 2813 Fragments arranged by size: 2813 2394 1199 1071 532 529 427 420 218 85 BsmI GAATG_Cn' Cuts at: 2443 3283 6228 7199 7292 2443 Size: 840 2945 971 93 4839 Fragments arranged by size: 4839 2945 971 840 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3329 3329 Size: 9688 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2457 2675 3204 4600 5598 6018 Size: 399 240 427 218 529 1396 998 420 Cuts at: 6018 6550 7007 7749 7834 8905 1391 Size: 532 457 742 85 1071 2174 Fragments arranged by size: 2174 1396 1071 998 742 532 529 457 427 420 399 240 218 85 BspEI T'CCGG_A Cuts at: 9351 9351 Size: 9688 BspHI T'CATG_A Cuts at: 2536 3033 3271 3793 5385 6838 7577 9307 2536 Size: 497 238 522 1592 1453 739 1730 2917 Fragments arranged by size: 2917 1730 1592 1453 739 522 497 238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3315 3398 4546 5091 7043 844 Size: 2471 83 1148 545 1952 3489 Fragments arranged by size: 3489 2471 1952 1148 545 83 BsrBI CCG'CTC Cuts at: 1394 1823 7575 1394 Size: 429 5752 3507 Fragments arranged by size: 5752 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2926 8183 8357 65 Size: 1183 1678 5257 174 1396 Fragments arranged by size: 5257 1678 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4648 5799 8332 8519 1700 Size: 110 2838 1151 2533 187 2869 Fragments arranged by size: 2869 2838 2533 1151 187 110 BsrGI T'GTAC_A Cuts at: 96 2096 96 Size: 2000 7688 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9688 BssSI C'ACGA_G Cuts at: 2493 7748 8760 2493 Size: 5255 1012 3421 Fragments arranged by size: 5255 3421 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 9688 BstBI TT'CG_AA Cuts at: 3514 6232 8492 3514 Size: 2718 2260 4710 Fragments arranged by size: 4710 2718 2260 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9688 BstXI CCAn_nnnn'nTGG Cuts at: 5446 5446 Size: 9688 BstYI r'GATC_y Cuts at: 1972 2193 2834 3466 5284 5380 5912 6397 6487 Size: 221 641 632 1818 96 532 485 90 Cuts at: 6487 6945 6961 7018 7385 7770 7787 9239 9325 Size: 458 16 57 367 385 17 1452 86 Cuts at: 9325 9337 9683 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1818 1452 641 632 532 485 458 385 367 346 221 96 90 86 57 17 16 12 Bsu36I CC'TnA_GG Cuts at: 2121 2121 Size: 9688 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 9136 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8949 Fragments arranged by size: 8949 683 56 BtsI GCAGTG_nn' Cuts at: 2618 3504 5476 7204 7998 8018 2618 Size: 886 1972 1728 794 20 4288 Fragments arranged by size: 4288 1972 1728 886 794 20 ClaI AT'CG_AT Cuts at: 1554 4953 1554 Size: 3399 6289 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9653 DraI TTT'AAA Cuts at: 2431 5343 6992 2431 Size: 2912 1649 5127 Fragments arranged by size: 5127 2912 1649 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8695 809 Size: 507 7379 1802 Fragments arranged by size: 7379 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4696 7135 8024 Size: 54 1425 50 275 18 2866 2439 889 Cuts at: 8024 9653 8 Size: 1629 43 Fragments arranged by size: 2866 2439 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7135 1537 Size: 275 5323 4090 Fragments arranged by size: 5323 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2460 3234 3801 3996 4443 4467 4920 6050 Size: 1107 774 567 195 447 24 453 1130 Cuts at: 6050 7618 1353 Size: 1568 3423 Fragments arranged by size: 3423 1568 1130 1107 774 567 453 447 195 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8275 8649 8795 387 Size: 1064 138 6686 374 146 1280 Fragments arranged by size: 6686 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2028 2455 2673 3202 5596 6548 2028 Size: 427 218 529 2394 952 5168 Fragments arranged by size: 5168 2394 952 529 427 218 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2295 2406 2571 2867 2877 3039 3176 Size: 393 663 111 165 296 10 162 137 Cuts at: 3176 4467 5108 5361 5466 5745 5902 5960 6015 Size: 1291 641 253 105 279 157 58 55 Cuts at: 6015 6231 6333 6677 7751 8348 9135 9403 1239 Size: 216 102 344 1074 597 787 268 1524 Fragments arranged by size: 1524 1291 1074 787 663 641 597 393 344 296 279 268 253 216 165 162 157 137 111 105 102 58 55 10 EcoNI CCTnn'n_nnAGG Cuts at: 5920 5920 Size: 9688 EcoO109I rG'GnC_Cy Cuts at: 2470 6443 2470 Size: 3973 5715 EcoRI G'AATT_C Cuts at: 2532 4614 6997 7375 2532 Size: 2082 2383 378 4845 Fragments arranged by size: 4845 2383 2082 378 EcoRV GAT'ATC Cuts at: 6208 6208 Size: 9688 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2489 5592 6278 6566 6707 2489 Size: 3103 686 288 141 5470 Fragments arranged by size: 5470 3103 686 288 141 FspI TGC'GCA Cuts at: 8194 8194 Size: 9688 HaeII r_GCGC'y Cuts at: 1678 2016 3548 8835 1678 Size: 338 1532 5287 2531 Fragments arranged by size: 5287 2531 1532 338 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2012 2044 2162 2194 3121 Size: 32 135 32 440 32 118 32 927 Cuts at: 3121 3153 3857 3889 4919 4951 6382 6414 6547 Size: 32 704 32 1030 32 1431 32 133 Cuts at: 6547 6579 6820 6852 8329 8361 8403 8435 1373 Size: 32 241 32 1477 32 42 32 2626 Fragments arranged by size: 2626 1477 1431 1030 927 704 440 241 135 133 118 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2317 5168 6429 7032 7278 669 Size: 988 281 379 2851 1261 603 246 3079 Fragments arranged by size: 3079 2851 1261 988 603 379 281 246 HindIII A'AGCT_T Cuts at: 748 5390 748 Size: 4642 5046 HpaI GTT'AAC Cuts at: 2317 6429 7278 2317 Size: 4112 849 4727 Fragments arranged by size: 4727 4112 849 KasI G'GCGC_C Cuts at: 2012 2012 Size: 9688 KpnI G_GTAC'C Cuts at: 7013 7013 Size: 9688 MfeI C'AATT_G Cuts at: 4229 7287 4229 Size: 3058 6630 MluI A'CGCG_T Cuts at: 9564 9564 Size: 9688 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4812 8802 8986 1305 Size: 3507 3990 184 2007 Fragments arranged by size: 3990 3507 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7866 Fragments arranged by size: 7866 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2702 3276 3798 4766 5444 5977 6524 Size: 648 1536 574 522 968 678 533 547 Cuts at: 6524 6594 7646 8005 8164 518 Size: 70 1052 359 159 2042 Fragments arranged by size: 2042 1536 1052 968 678 648 574 547 533 522 359 159 70 MspA1I CmG'CkG Cuts at: 1606 3558 4565 6893 7104 7781 8929 9174 1606 Size: 1952 1007 2328 211 677 1148 245 2120 Fragments arranged by size: 2328 2120 1952 1148 1007 677 245 211 NaeI GCC'GGC Cuts at: 4650 5801 4650 Size: 1151 8537 NarI GG'CG_CC Cuts at: 2013 2013 Size: 9688 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 9136 NdeI CA'TA_TG Cuts at: 387 5220 5704 6860 387 Size: 4833 484 1156 3215 Fragments arranged by size: 4833 3215 1156 484 NgoMIV G'CCGG_C Cuts at: 4648 5799 4648 Size: 1151 8537 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9688 NotI GC'GGCC_GC Cuts at: 7135 7135 Size: 9688 NsiI A_TGCA'T Cuts at: 3349 4585 3349 Size: 1236 8452 NspI r_CATG'y Cuts at: 1132 1229 2687 2966 3045 3268 4193 7046 8591 Size: 97 1458 279 79 223 925 2853 1545 Cuts at: 8591 1132 Size: 2229 Fragments arranged by size: 2853 2229 1545 1458 925 279 223 97 79 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9688 PciI A'CATG_T Cuts at: 2962 3264 8587 2962 Size: 302 5323 4063 Fragments arranged by size: 5323 4063 302 PflMI CCAn_nnn'nTGG Cuts at: 1108 4426 1108 Size: 3318 6370 PfoI T'CCnGG_A Cuts at: 2636 2636 Size: 9688 PmeI GTTT'AAAC Cuts at: 6992 6992 Size: 9688 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2662 2694 5425 5457 7757 7789 9270 9302 2662 Size: 32 2731 32 2300 32 1481 32 3048 Fragments arranged by size: 3048 2731 2300 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2470 2470 Size: 9688 PshAI GACnn'nnGTC Cuts at: 1613 4483 1613 Size: 2870 6818 PsiI TTA'TAA Cuts at: 7258 7258 Size: 9688 PstI C_TGCA'G Cuts at: 830 3981 4164 5047 6776 6892 7040 830 Size: 3151 183 883 1729 116 148 3478 Fragments arranged by size: 3478 3151 1729 883 183 148 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9688 PvuII CAG'CTG Cuts at: 4565 6893 4565 Size: 2328 7360 SacI G_AGCT'C Cuts at: 2030 2457 2675 3204 5598 6550 2030 Size: 427 218 529 2394 952 5168 Fragments arranged by size: 5168 2394 952 529 427 218 SalI G'TCGA_C Cuts at: 1936 5166 7030 1936 Size: 3230 1864 4594 Fragments arranged by size: 4594 3230 1864 SapI GCTCTTCn'nnn_ Cuts at: 4467 4467 Size: 9688 SbfI CC_TGCA'GG Cuts at: 7040 7040 Size: 9688 ScaI AGT'ACT Cuts at: 5157 5323 7936 9648 5157 Size: 166 2613 1712 5197 Fragments arranged by size: 5197 2613 1712 166 SexAI A'CCwGG_T Cuts at: 4056 4056 Size: 9688 SfcI C'TryA_G Cuts at: 826 1045 2250 2862 2971 2999 3287 3977 4160 Size: 219 1205 612 109 28 288 690 183 Cuts at: 4160 5043 6417 6772 6888 7036 8171 8852 9043 Size: 883 1374 355 116 148 1135 681 191 Cuts at: 9043 826 Size: 1471 Fragments arranged by size: 1471 1374 1205 1135 883 690 681 612 355 288 219 191 183 148 116 109 28 SfoI GGC'GCC Cuts at: 2014 2014 Size: 9688 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9688 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9688 SmaI CCC'GGG Cuts at: 1872 7015 1872 Size: 5143 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2349 4742 4817 7792 8693 8955 Size: 197 926 406 2393 75 2975 901 262 Cuts at: 8955 820 Size: 1553 Fragments arranged by size: 2975 2393 1553 926 901 406 262 197 75 SnaBI TAC'GTA Cuts at: 493 7479 493 Size: 6986 2702 SpeI A'CTAG_T Cuts at: 152 5489 152 Size: 5337 4351 SphI G_CATG'C Cuts at: 1132 7046 1132 Size: 5914 3774 SspI AAT'ATT Cuts at: 5 52 7612 5 Size: 47 7560 2081 Fragments arranged by size: 7560 2081 47 StuI AGG'CCT Cuts at: 1517 4637 1517 Size: 3120 6568 StyI C'CwwG_G Cuts at: 513 1065 2131 2934 5730 6439 6507 6732 7127 Size: 552 1066 803 2796 709 68 225 395 Cuts at: 7127 513 Size: 3074 Fragments arranged by size: 3074 2796 1066 803 709 552 395 225 68 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 6584 6768 6890 7715 7732 7885 Size: 1255 1035 3969 184 122 825 17 153 Cuts at: 7885 8070 8515 325 Size: 185 445 1498 Fragments arranged by size: 3969 1498 1255 1035 825 445 185 184 153 122 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2096 3210 5155 Size: 274 80 33 51 1443 119 1114 1945 Cuts at: 5155 5321 5492 7934 9646 96 Size: 166 171 2442 1712 138 Fragments arranged by size: 2442 1945 1712 1443 1114 274 171 166 138 119 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5900 5932 9648 9680 5900 Size: 32 3716 32 5908 Fragments arranged by size: 5908 3716 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4327 1316 Size: 3011 6677 XbaI T'CTAG_A Cuts at: 4331 5287 6644 7024 4331 Size: 956 1357 380 6995 Fragments arranged by size: 6995 1357 956 380 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4789 5443 4789 Size: 654 9034 XhoI C'TCGA_G Cuts at: 1943 2349 1943 Size: 406 9282 XmaI C'CCGG_G Cuts at: 1870 7013 1870 Size: 5143 4545 XmnI GAAnn'nnTTC Cuts at: 4428 7817 4428 Size: 3389 6299 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7496 276 Size: 53 83 186 936 5962 2468 Fragments arranged by size: 5962 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbvCI BplI BsiWI BstZ17I DraIII FseI FspAI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI