(Circular) (Six-base) MAPSORT of: pFN21AA1707.seq Check: 7017 from: 1 to: 7756 pFN21AA1707 7756 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5566 278 Size: 53 83 186 936 4030 2468 Fragments arranged by size: 4030 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5099 1937 Size: 3162 4594 Acc65I G'GTAC_C Cuts at: 5077 5077 Size: 7756 AclI AA'CG_TT Cuts at: 1563 5883 6256 1563 Size: 4320 373 3063 Fragments arranged by size: 4320 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2718 2985 3669 4002 5819 7203 7471 1632 Size: 1086 267 684 333 1817 1384 268 1917 Fragments arranged by size: 1917 1817 1384 1086 684 333 268 267 AflII C'TTAA_G Cuts at: 820 1017 3278 4259 820 Size: 197 2261 981 4317 Fragments arranged by size: 4317 2261 981 197 AflIII A'CryG_T Cuts at: 1203 2339 3365 6655 7632 1203 Size: 1136 1026 3290 977 1327 Fragments arranged by size: 3290 1327 1136 1026 977 AgeI A'CCGG_T Cuts at: 6587 6587 Size: 7756 AhdI GACnn_n'nnGTC Cuts at: 6485 6485 Size: 7756 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7756 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7756 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3083 3115 3083 Size: 32 7724 AlwNI CAG_nnn'CTG Cuts at: 5499 7071 5499 Size: 1572 6184 ApaLI G'TGCA_C Cuts at: 5813 6969 5813 Size: 1156 6600 ApoI r'AATT_y Cuts at: 1493 2054 2240 3273 3755 4759 5065 5291 5443 Size: 561 186 1033 482 1004 306 226 152 Cuts at: 5443 7552 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1033 1004 561 482 306 226 186 152 AseI AT'TA_AT Cuts at: 160 1032 6310 160 Size: 872 5278 1606 Fragments arranged by size: 5278 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7756 AvaI C'yCGr_G Cuts at: 1870 1943 5081 1870 Size: 73 3138 4545 Fragments arranged by size: 4545 3138 73 BamHI G'GATC_C Cuts at: 2950 4921 5086 5453 2950 Size: 1971 165 367 5253 Fragments arranged by size: 5253 1971 367 165 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5077 6532 618 Size: 325 217 627 3290 1455 1842 Fragments arranged by size: 3290 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2588 5013 5075 1391 Size: 1197 2425 62 4072 Fragments arranged by size: 4072 2425 1197 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2706 2813 4405 928 Size: 971 807 107 1592 4279 Fragments arranged by size: 4279 1592 971 807 107 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5947 5981 1903 Size: 34 4010 34 3678 Fragments arranged by size: 4010 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2382 3586 4564 5648 6858 2382 Size: 1204 978 1084 1210 3280 Fragments arranged by size: 3280 1210 1204 1084 978 BclI T'GATC_A Cuts at: 1548 2728 1548 Size: 1180 6576 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6367 136 Size: 107 122 71 631 605 4695 1525 Fragments arranged by size: 4695 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4201 7751 4201 Size: 3550 4206 BlpI GC'TnA_GC Cuts at: 4572 5173 4572 Size: 601 7155 Bme1580I G_kGCm'C Cuts at: 1790 5817 6973 1790 Size: 4027 1156 2573 Fragments arranged by size: 4027 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7756 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5487 6445 442 Size: 416 53 83 399 24 4070 958 1753 Fragments arranged by size: 4070 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7756 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4226 6416 1239 Size: 2987 2190 2579 Fragments arranged by size: 2987 2579 2190 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5881 6746 7044 5881 Size: 865 298 6593 Fragments arranged by size: 6593 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3685 6419 882 Size: 617 2186 2734 2219 Fragments arranged by size: 2734 2219 2186 617 BsaAI yAC'GTr Cuts at: 493 1204 4616 5547 493 Size: 711 3412 931 2702 Fragments arranged by size: 3412 2702 931 711 BsaBI GATnn'nnATC Cuts at: 3639 4608 7750 3639 Size: 969 3142 3645 Fragments arranged by size: 3645 3142 969 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5563 5945 6580 275 Size: 53 83 186 936 4030 382 635 1451 Fragments arranged by size: 4030 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6189 6587 6861 7008 7419 6189 Size: 398 274 147 411 6526 Fragments arranged by size: 6526 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4949 4979 1178 Size: 30 165 30 3546 30 3955 Fragments arranged by size: 3955 3546 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7756 BseYI C'CCAG_C Cuts at: 4557 6959 7455 4557 Size: 2402 496 4858 Fragments arranged by size: 4858 2402 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4662 1163 Size: 573 2926 4257 Fragments arranged by size: 4257 2926 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5206 5967 6995 1540 Size: 275 186 3205 761 1028 2301 Fragments arranged by size: 3205 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5013 5817 5902 6973 5013 Size: 804 85 1071 5796 Fragments arranged by size: 5796 1071 804 85 BsmI GAATG_Cn' Cuts at: 5267 5360 5267 Size: 93 7663 BsmBI CGTCTCn'nnnn_ Cuts at: 4485 4485 Size: 7756 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2588 5013 5075 5817 5902 6973 1391 Size: 399 798 2425 62 742 85 1071 2174 Fragments arranged by size: 2425 2174 1071 798 742 399 85 62 BspEI T'CCGG_A Cuts at: 7419 7419 Size: 7756 BspHI T'CATG_A Cuts at: 5645 7375 5645 Size: 1730 6026 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5111 844 Size: 4267 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5643 1394 Size: 429 3820 3507 Fragments arranged by size: 3820 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2306 3581 6251 6425 65 Size: 1183 1058 1275 2670 174 1396 Fragments arranged by size: 2670 1396 1275 1183 1058 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3065 6400 6587 1700 Size: 110 1255 3335 187 2869 Fragments arranged by size: 3335 2869 1255 187 110 BsrGI T'GTAC_A Cuts at: 96 3376 4741 96 Size: 3280 1365 3111 Fragments arranged by size: 3280 3111 1365 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7756 BssSI C'ACGA_G Cuts at: 5816 6828 5816 Size: 1012 6744 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7756 BstBI TT'CG_AA Cuts at: 6560 6560 Size: 7756 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7756 BstYI r'GATC_y Cuts at: 1972 2950 3454 3549 4201 4837 4921 5086 5453 Size: 978 504 95 652 636 84 165 367 Cuts at: 5453 5838 5855 7307 7393 7405 7751 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 978 652 636 504 385 367 346 165 95 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 2105 2105 Size: 7756 BtgI C'CryG_G Cuts at: 513 1065 4511 513 Size: 552 3446 3758 Fragments arranged by size: 3758 3446 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7017 Fragments arranged by size: 7017 683 56 BtsI GCAGTG_nn' Cuts at: 2096 4879 5272 6066 6086 2096 Size: 2783 393 794 20 3766 Fragments arranged by size: 3766 2783 794 393 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7756 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2902 2937 4815 4850 529 Size: 35 2338 35 1878 35 3435 Fragments arranged by size: 3435 2338 1878 35 35 35 DraI TTT'AAA Cuts at: 3606 5060 3606 Size: 1454 6302 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6763 809 Size: 507 5447 1802 Fragments arranged by size: 5447 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4029 5203 6092 Size: 54 1425 50 275 18 2199 1174 889 Cuts at: 6092 7721 8 Size: 1629 43 Fragments arranged by size: 2199 1629 1425 1174 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5203 1537 Size: 275 3391 4090 Fragments arranged by size: 4090 3391 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2364 3688 3913 5686 1353 Size: 1011 1324 225 1773 3423 Fragments arranged by size: 3423 1773 1324 1011 225 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6343 6717 6863 387 Size: 1064 138 4754 374 146 1280 Fragments arranged by size: 4754 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5011 5011 Size: 7756 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2718 2985 3669 4002 4226 5819 6416 Size: 393 1086 267 684 333 224 1593 597 Cuts at: 6416 7203 7471 1239 Size: 787 268 1524 Fragments arranged by size: 1593 1524 1086 787 684 597 393 333 268 267 224 EcoO109I rG'GnC_Cy Cuts at: 2448 3248 2448 Size: 800 6956 EcoRI G'AATT_C Cuts at: 3755 5065 5443 3755 Size: 1310 378 6068 Fragments arranged by size: 6068 1310 378 FspI TGC'GCA Cuts at: 2634 6262 2634 Size: 3628 4128 HaeII r_GCGC'y Cuts at: 1678 6903 1678 Size: 5225 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4141 4173 4268 4300 4998 Size: 32 135 32 2569 32 95 32 698 Cuts at: 4998 5030 6397 6429 6471 6503 1373 Size: 32 1367 32 42 32 2626 Fragments arranged by size: 2626 2569 1367 698 135 95 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2208 2326 2871 3033 4933 5100 Size: 988 281 270 118 545 162 1900 167 Cuts at: 5100 5346 669 Size: 246 3079 Fragments arranged by size: 3079 1900 988 545 281 270 246 167 162 118 HindIII A'AGCT_T Cuts at: 748 4909 748 Size: 4161 3595 HpaI GTT'AAC Cuts at: 4933 5346 4933 Size: 413 7343 KpnI G_GTAC'C Cuts at: 5081 5081 Size: 7756 MfeI C'AATT_G Cuts at: 3222 5355 3222 Size: 2133 5623 MluI A'CGCG_T Cuts at: 7632 7632 Size: 7756 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6870 7054 1305 Size: 5565 184 2007 Fragments arranged by size: 5565 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5934 Fragments arranged by size: 5934 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5714 6073 6232 518 Size: 648 4548 359 159 2042 Fragments arranged by size: 4548 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3215 5172 5849 6997 7242 1606 Size: 1609 1957 677 1148 245 2120 Fragments arranged by size: 2120 1957 1609 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 4511 513 Size: 552 3446 3758 Fragments arranged by size: 3758 3446 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7756 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7756 NotI GC'GGCC_GC Cuts at: 5203 5203 Size: 7756 NspI r_CATG'y Cuts at: 1132 1229 2343 3369 5114 6659 1132 Size: 97 1114 1026 1745 1545 2229 Fragments arranged by size: 2229 1745 1545 1114 1026 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7756 PciI A'CATG_T Cuts at: 2339 3365 6655 2339 Size: 1026 3290 3440 Fragments arranged by size: 3440 3290 1026 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7756 PfoI T'CCnGG_A Cuts at: 2801 2801 Size: 7756 PmeI GTTT'AAAC Cuts at: 5060 5060 Size: 7756 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4408 4440 5825 5857 7338 7370 4408 Size: 32 1385 32 1481 32 4794 Fragments arranged by size: 4794 1481 1385 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2448 3248 2448 Size: 800 6956 PshAI GACnn'nnGTC Cuts at: 1613 2213 2411 1613 Size: 600 198 6958 Fragments arranged by size: 6958 600 198 PsiI TTA'TAA Cuts at: 5326 5326 Size: 7756 PstI C_TGCA'G Cuts at: 830 4056 5108 830 Size: 3226 1052 3478 Fragments arranged by size: 3478 3226 1052 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7756 PvuII CAG'CTG Cuts at: 3215 3215 Size: 7756 SacI G_AGCT'C Cuts at: 5013 5013 Size: 7756 SalI G'TCGA_C Cuts at: 1936 5098 1936 Size: 3162 4594 SbfI CC_TGCA'GG Cuts at: 5108 5108 Size: 7756 ScaI AGT'ACT Cuts at: 3328 6004 7716 3328 Size: 2676 1712 3368 Fragments arranged by size: 3368 2676 1712 SfcI C'TryA_G Cuts at: 826 1045 4052 5104 6239 6920 7111 826 Size: 219 3007 1052 1135 681 191 1471 Fragments arranged by size: 3007 1471 1135 1052 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7756 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7756 SmaI CCC'GGG Cuts at: 1872 5083 1872 Size: 3211 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3278 4259 5860 6761 7023 820 Size: 197 926 1335 981 1601 901 262 1553 Fragments arranged by size: 1601 1553 1335 981 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4616 5547 493 Size: 4123 931 2702 Fragments arranged by size: 4123 2702 931 SpeI A'CTAG_T Cuts at: 152 152 Size: 7756 SphI G_CATG'C Cuts at: 1132 5114 1132 Size: 3982 3774 SspI AAT'ATT Cuts at: 5 52 2768 2849 5680 5 Size: 47 2716 81 2831 2081 Fragments arranged by size: 2831 2716 2081 81 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7756 StyI C'CwwG_G Cuts at: 513 1065 4511 5195 513 Size: 552 3446 684 3074 Fragments arranged by size: 3446 3074 684 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5783 5800 5953 6138 6583 325 Size: 1255 4203 17 153 185 445 1498 Fragments arranged by size: 4203 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2852 3326 3376 Size: 274 80 33 51 1443 875 474 50 Cuts at: 3376 4741 6002 7714 96 Size: 1365 1261 1712 138 Fragments arranged by size: 1712 1443 1365 1261 875 474 274 138 80 51 50 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7716 7748 7716 Size: 32 7724 Tth111I GACn'n_nGTC Cuts at: 1316 4630 1316 Size: 3314 4442 XbaI T'CTAG_A Cuts at: 2125 5092 2125 Size: 2967 4789 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3109 4107 3109 Size: 998 6758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7756 XmaI C'CCGG_G Cuts at: 1870 5081 1870 Size: 3211 4545 XmnI GAAnn'nnTTC Cuts at: 2358 4539 5001 5885 2358 Size: 2181 462 884 4229 Fragments arranged by size: 4229 2181 884 462 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5564 276 Size: 53 83 186 936 4030 2468 Fragments arranged by size: 4030 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI