Restriction map for
pFN21AA1685
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbeI BfrI BlnI BsaHI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII Eam1105I Eco81I EcoO65I EcoRV
EcoT22I FseI FspI Hin1I HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SfiI SfoI SgrAI SmaI SnaBI
Sse8387I SspBI SwaI XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 1755 ACGTGT A^C[GA][CT]GT
Aor51HI 2973 AGCGCT AGC^GCT
BalI 4134 TGGCCA TGG^CCA
BanI 2527 GGTGCC G^G[CT][GA]CC
BbrPI 1754 CACGTG CAC^GTG
BcgI 2750 CGAGCACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2834 GTATCC GTATCC(6/5)
BglI 2437 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 754 AGATCT A^GATCT
BsaAI 1754 CACGTG [CT]AC^GT[GA]
BsaBI 1453 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 819 ACCGGA [AT]^CCGG[AT]
BsiEI 3393 CGGCCG CG[GA][CT]^CG
BsmBI 4284 CGTCTC CGTCTC(1/5)
BsrBI 1628 GAGCGG CCGCTC(-3/-3)
BstBI 4191 TTCGAA TT^CGAA
BstXI 3606 CCAGAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 2796 TTTAAA TTT^AAA
DrdI 935 GACAGGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 3393 CGGCCG C^GGCCG
EclXI 3393 CGGCCG C^GGCCG
Eco47III 2973 AGCGCT AGC^GCT
Eco52I 3393 CGGCCG C^GGCCG
EcoNI 2077 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 4283 GCGTC GACGC(5/10)
HindIII 2692 AAGCTT A^AGCTT
MamI 1453 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4134 TGGCCA TGG^CCA
MscI 4134 TGGCCA TGG^CCA
NspV 4191 TTCGAA TT^CGAA
PaeR7I 2748 CTCGAG C^TCGAG
PmaCI 1754 CACGTG CAC^GTG
PmlI 1754 CACGTG CAC^GTG
SacI 2566 GAGCTC GAGCT^C
ScaI 3553 AGTACT AGT^ACT
SexAI 4035 ACCAGGT A^CC[AT]GGT
SfuI 4191 TTCGAA TT^CGAA
SpeI 1939 ACTAGT A^CTAGT
SspI 3379 AATATT AAT^ATT
StuI 1247 AGGCCT AGG^CCT
XhoI 2748 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
ApaI 2175 GGGCCC GGGCC^C
2467 GGGCCC
AspI 2093 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2152 GACAGAGTC
AvaI 2748 CTCGAG C^[CT]CG[GA]G
3856 CCCGAG
BbsI 3292 GAAGAC GAAGAC(2/6)
1707 GTCTTC
BbvCI 1397 CCTCAGC CCTCAGC(-5/-2)
3129 GCTGAGG
BcnI 1863 CCCGG CC^[GC]GG
3396 CCGGG
BpuAI 3292 GAAGAC GAAGAC(2/6)
1707 GTCTTC
BsgI 1357 GTGCAG GTGCAG(16/14)
2546 GTGCAG
BsmI 168 GAATGC GAATGC(1/-1)
1427 GCATTC
BsoBI 2748 CTCGAG C^[CT]CG[GA]G
3856 CCCGAG
EaeI 3393 CGGCCG [CT]^GGCC[GA]
4134 TGGCCA
EcoRI 988 GAATTC G^AATTC
1064 GAATTC
FauI 417 CCCGC CCCGC(4/6)
1630 GCGGG
MslI 1233 CATGCCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4218 CATGATCATG
NciI 1863 CCCGG CC^[GC]GG
3396 CCGGG
NheI 3679 GCTAGC G^CTAGC
4105 GCTAGC
NspI 160 GCATGC [GA]CATG^[CT]
2362 GCATGC
PflFI 2093 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2152 GACAGAGTC
PflMI 2071 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2083 CCAGGGGGTGG
PstI 2042 CTGCAG CTGCA^G
3890 CTGCAG
SphI 160 GCATGC GCATG^C
2362 GCATGC
Tth111I 2093 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2152 GACAGAGTC
Van91I 2071 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2083 CCAGGGGGTGG
XcmI 2180 CCACACTGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3347 CCAAACCTGCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanII BbvI BclI BfaI BlpI
BmyI BpmI Bpu1102I BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI