(Circular) (Six-base) MAPSORT of: pFN21AA1685.seq Check: 8602 from: 1 to: 9010 pFN21AA1685 9010 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2382 3052 4360 4741 2382 Size: 670 1308 381 6651 Fragments arranged by size: 6651 1308 670 381 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6820 278 Size: 53 83 186 936 5284 2468 Fragments arranged by size: 5284 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6353 1937 Size: 4416 4594 Acc65I G'GTAC_C Cuts at: 6331 6331 Size: 9010 AclI AA'CG_TT Cuts at: 1563 7137 7510 1563 Size: 5574 373 3063 Fragments arranged by size: 5574 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2988 3369 4593 5096 5189 5463 5493 7073 Size: 1356 381 1224 503 93 274 30 1580 Cuts at: 7073 8457 8725 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1580 1384 1356 1224 503 381 274 268 93 30 AfeI AGC'GCT Cuts at: 4980 4980 Size: 9010 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8813 AflIII A'CryG_T Cuts at: 1203 3760 7909 8886 1203 Size: 2557 4149 977 1327 Fragments arranged by size: 4149 2557 1327 977 AgeI A'CCGG_T Cuts at: 7841 7841 Size: 9010 AhdI GACnn_n'nnGTC Cuts at: 7739 7739 Size: 9010 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9010 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5090 1184 Size: 3906 5104 AlwNI CAG_nnn'CTG Cuts at: 2040 4025 4046 4082 4205 4517 4646 4733 5062 Size: 1985 21 36 123 312 129 87 329 Cuts at: 5062 6092 6753 8325 2040 Size: 1030 661 1572 2725 Fragments arranged by size: 2725 1985 1572 1030 661 329 312 129 123 87 36 21 ApaI G_GGCC'C Cuts at: 4184 4476 4184 Size: 292 8718 ApaLI G'TGCA_C Cuts at: 7067 8223 7067 Size: 1156 7854 ApoI r'AATT_y Cuts at: 1493 2103 2993 3069 4902 6319 6545 6697 8806 Size: 610 890 76 1833 1417 226 152 2109 Cuts at: 8806 1493 Size: 1697 Fragments arranged by size: 2109 1833 1697 1417 890 610 226 152 76 AseI AT'TA_AT Cuts at: 160 1032 7564 160 Size: 872 6532 1606 Fragments arranged by size: 6532 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9010 AvaI C'yCGr_G Cuts at: 1870 1943 4753 5861 6335 1870 Size: 73 2810 1108 474 4545 Fragments arranged by size: 4545 2810 1108 474 73 BamHI G'GATC_C Cuts at: 6340 6707 6340 Size: 367 8643 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4532 6331 7786 618 Size: 325 217 627 2745 1799 1455 1842 Fragments arranged by size: 2745 1842 1799 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3393 3692 3925 4184 4476 4575 5408 5601 Size: 2002 299 233 259 292 99 833 193 Cuts at: 5601 6329 1391 Size: 728 4072 Fragments arranged by size: 4072 2002 833 728 299 292 259 233 193 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3705 5304 928 Size: 971 1806 1599 4634 Fragments arranged by size: 4634 1806 1599 971 BbvCI CC'TCA_GC Cuts at: 3403 5135 3403 Size: 1732 7278 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4744 4778 7201 7235 1903 Size: 34 2807 34 2423 34 3678 Fragments arranged by size: 3678 2807 2423 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4850 6902 8112 4850 Size: 2052 1210 5748 Fragments arranged by size: 5748 2052 1210 BclI T'GATC_A Cuts at: 1548 2862 3682 5994 6225 1548 Size: 1314 820 2312 231 4333 Fragments arranged by size: 4333 2312 1314 820 231 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4448 7621 136 Size: 107 122 71 631 605 2776 3173 1525 Fragments arranged by size: 3173 2776 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2759 9005 2759 Size: 6246 2764 BlpI GC'TnA_GC Cuts at: 2977 3425 5680 6427 2977 Size: 448 2255 747 5560 Fragments arranged by size: 5560 2255 747 448 Bme1580I G_kGCm'C Cuts at: 1790 3452 4184 4476 5373 6191 7071 8227 1790 Size: 1662 732 292 897 818 880 1156 2573 Fragments arranged by size: 2573 1662 1156 897 880 818 732 292 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9010 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3186 3280 4255 Size: 416 53 83 399 24 1769 94 975 Cuts at: 4255 4331 4470 6741 7699 442 Size: 76 139 2271 958 1753 Fragments arranged by size: 2271 1769 1753 975 958 416 399 139 94 83 76 53 24 BmtI G_CTAG'C Cuts at: 1056 5688 6114 1056 Size: 4632 426 3952 Fragments arranged by size: 4632 3952 426 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2179 2677 2913 3859 4332 5128 7670 1239 Size: 940 498 236 946 473 796 2542 2579 Fragments arranged by size: 2579 2542 946 940 796 498 473 236 Bpu10I CC'TnA_GC Cuts at: 3155 3403 5127 5135 3155 Size: 248 1724 8 7030 Fragments arranged by size: 7030 1724 248 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3686 3845 7135 8000 8298 3686 Size: 159 3290 865 298 4398 Fragments arranged by size: 4398 3290 865 298 159 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3805 3877 3916 4262 7673 882 Size: 617 2306 72 39 346 3411 2219 Fragments arranged by size: 3411 2306 2219 617 346 72 39 BsaAI yAC'GTr Cuts at: 493 1204 3761 6801 493 Size: 711 2557 3040 2702 Fragments arranged by size: 3040 2702 2557 711 BsaBI GATnn'nnATC Cuts at: 3462 9004 3462 Size: 5542 3468 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6817 7199 7834 275 Size: 53 83 186 936 5284 382 635 1451 Fragments arranged by size: 5284 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2824 7443 7841 8115 8262 8673 2824 Size: 4619 398 274 147 411 3161 Fragments arranged by size: 4619 3161 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2177 2207 2675 2705 3172 Size: 30 165 30 774 30 468 30 467 Cuts at: 3172 3202 1178 Size: 30 6986 Fragments arranged by size: 6986 774 468 467 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2148 2560 4152 5609 1186 Size: 962 412 1592 1457 4587 Fragments arranged by size: 4587 1592 1457 962 412 BseYI C'CCAG_C Cuts at: 3217 3744 5062 5326 6128 8213 8709 3217 Size: 527 1318 264 802 2085 496 3518 Fragments arranged by size: 3518 2085 1318 802 527 496 264 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3383 4572 1163 Size: 573 1647 1189 5601 Fragments arranged by size: 5601 1647 1189 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5401 6460 7221 8249 1540 Size: 275 186 3400 1059 761 1028 2301 Fragments arranged by size: 3400 2301 1059 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2860 4318 4575 4760 7071 7156 8227 2860 Size: 1458 257 185 2311 85 1071 3643 Fragments arranged by size: 3643 2311 1458 1071 257 185 85 BsmI GAATG_Cn' Cuts at: 2179 3432 6521 6614 2179 Size: 1253 3089 93 4575 Fragments arranged by size: 4575 3089 1253 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6295 6295 Size: 9010 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2860 3393 3452 3692 3925 4184 4318 Size: 399 1070 533 59 240 233 259 134 Cuts at: 4318 4476 4575 4760 5373 5408 5601 6191 6329 Size: 158 99 185 613 35 193 590 138 Cuts at: 6329 7071 7156 8227 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1070 742 613 590 533 399 259 240 233 193 185 158 138 134 99 85 59 35 BspEI T'CCGG_A Cuts at: 8673 8673 Size: 9010 BspHI T'CATG_A Cuts at: 6899 8629 6899 Size: 1730 7280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2382 2447 2632 2641 3052 3898 4360 4571 Size: 1538 65 185 9 411 846 462 211 Cuts at: 4571 4741 5365 6365 844 Size: 170 624 1000 3489 Fragments arranged by size: 3489 1538 1000 846 624 462 411 211 185 170 65 9 BsrBI CCG'CTC Cuts at: 1394 1823 3635 6897 1394 Size: 429 1812 3262 3507 Fragments arranged by size: 3507 3262 1812 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2184 2552 3308 5367 6067 7505 7679 Size: 1183 936 368 756 2059 700 1438 174 Cuts at: 7679 65 Size: 1396 Fragments arranged by size: 2059 1438 1396 1183 936 756 700 368 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7654 7841 1700 Size: 110 5844 187 2869 Fragments arranged by size: 5844 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9010 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9010 BssSI C'ACGA_G Cuts at: 7070 8082 7070 Size: 1012 7998 BstAPI GCAn_nnn'nTGC Cuts at: 794 2911 4025 4046 4906 794 Size: 2117 1114 21 860 4898 Fragments arranged by size: 4898 2117 1114 860 21 BstBI TT'CG_AA Cuts at: 6197 7814 6197 Size: 1617 7393 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9010 BstXI CCAn_nnnn'nTGG Cuts at: 5618 5618 Size: 9010 BstYI r'GATC_y Cuts at: 1972 2759 3463 3864 5199 6340 6707 7092 7109 Size: 787 704 401 1335 1141 367 385 17 Cuts at: 7109 8561 8647 8659 9005 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1335 1141 787 704 401 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3107 4893 6136 513 Size: 552 2042 1786 1243 3387 Fragments arranged by size: 3387 2042 1786 1243 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3558 3888 487 Size: 683 56 2332 330 5609 Fragments arranged by size: 5609 2332 683 330 56 BtsI GCAGTG_nn' Cuts at: 2314 2362 5838 6526 7320 7340 2314 Size: 48 3476 688 794 20 3984 Fragments arranged by size: 3984 3476 794 688 48 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9010 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6223 6258 529 Size: 35 5659 35 3281 Fragments arranged by size: 5659 3281 35 35 DraI TTT'AAA Cuts at: 4803 6314 4803 Size: 1511 7499 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2946 8017 809 Size: 507 1630 5071 1802 Fragments arranged by size: 5071 1802 1630 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5398 6139 6457 Size: 54 1425 50 275 18 3568 741 318 Cuts at: 6457 7346 8975 8 Size: 889 1629 43 Fragments arranged by size: 3568 1629 1425 889 741 318 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5398 6457 1537 Size: 275 3586 1059 4090 Fragments arranged by size: 4090 3586 1059 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2199 3333 3723 3746 6165 6940 1353 Size: 846 1134 390 23 2419 775 3423 Fragments arranged by size: 3423 2419 1134 846 775 390 23 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2060 7597 7971 8117 387 Size: 1064 138 471 5537 374 146 1280 Fragments arranged by size: 5537 1280 1064 471 374 146 138 EcoICRI GAG'CTC Cuts at: 4573 4573 Size: 9010 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2179 2677 2913 2988 3369 3859 4332 Size: 393 547 498 236 75 381 490 473 Cuts at: 4332 4593 5096 5128 5189 5463 5493 7073 7670 Size: 261 503 32 61 274 30 1580 597 Cuts at: 7670 8457 8725 1239 Size: 787 268 1524 Fragments arranged by size: 1580 1524 787 597 547 503 498 490 473 393 381 274 268 261 236 75 61 32 30 EcoNI CCTnn'n_nnAGG Cuts at: 4086 4086 Size: 9010 EcoO109I rG'GnC_Cy Cuts at: 2037 3733 4079 4180 4181 4472 4514 4583 5106 Size: 1696 346 101 1 291 42 69 523 Cuts at: 5106 5424 5642 2037 Size: 318 218 5405 Fragments arranged by size: 5405 1696 523 346 318 291 218 101 69 42 1 EcoRI G'AATT_C Cuts at: 2993 3069 6319 6697 2993 Size: 76 3250 378 5306 Fragments arranged by size: 5306 3250 378 76 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2080 2123 2169 2080 Size: 43 46 8921 Fragments arranged by size: 8921 46 43 FspI TGC'GCA Cuts at: 7516 7516 Size: 9010 HaeII r_GCGC'y Cuts at: 1678 2513 4982 6249 8157 1678 Size: 835 2469 1267 1908 2531 Fragments arranged by size: 2531 2469 1908 1267 835 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2143 2175 2303 2335 2847 Size: 32 135 32 571 32 128 32 512 Cuts at: 2847 2879 3172 3204 5021 5053 5221 5253 5561 Size: 32 293 32 1817 32 168 32 308 Cuts at: 5561 5593 5979 6011 6014 6046 6062 6094 7651 Size: 32 386 32 3 32 16 32 1557 Cuts at: 7651 7683 7725 7757 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1817 1557 571 512 386 308 293 168 135 128 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 16 3 HincII GTy'rAC Cuts at: 669 1657 1938 6354 6600 669 Size: 988 281 4416 246 3079 Fragments arranged by size: 4416 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 4697 748 Size: 3949 5061 HpaI GTT'AAC Cuts at: 6600 6600 Size: 9010 KpnI G_GTAC'C Cuts at: 6335 6335 Size: 9010 MfeI C'AATT_G Cuts at: 6609 6609 Size: 9010 MluI A'CGCG_T Cuts at: 8886 8886 Size: 9010 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2598 4042 4370 4532 8124 8308 1305 Size: 1293 1444 328 162 3592 184 2007 Fragments arranged by size: 3592 2007 1444 1293 328 184 162 MscI TGG'CCA Cuts at: 10 64 1489 1832 6141 10 Size: 54 1425 343 4309 2879 Fragments arranged by size: 4309 2879 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3242 6227 6968 7327 7486 518 Size: 648 2076 2985 741 359 159 2042 Fragments arranged by size: 2985 2076 2042 741 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3221 4025 4031 4043 4556 4870 5062 5293 Size: 1615 804 6 12 513 314 192 231 Cuts at: 5293 6426 7103 8251 8496 1606 Size: 1133 677 1148 245 2120 Fragments arranged by size: 2120 1615 1148 1133 804 677 513 314 245 231 192 12 6 NcoI C'CATG_G Cuts at: 513 1065 3107 4893 6136 513 Size: 552 2042 1786 1243 3387 Fragments arranged by size: 3387 2042 1786 1243 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9010 NheI G'CTAG_C Cuts at: 1052 5684 6110 1052 Size: 4632 426 3952 Fragments arranged by size: 4632 3952 426 NotI GC'GGCC_GC Cuts at: 6457 6457 Size: 9010 NspI r_CATG'y Cuts at: 1132 1229 2169 4371 6368 7913 1132 Size: 97 940 2202 1997 1545 2229 Fragments arranged by size: 2229 2202 1997 1545 940 97 PasI CC'CwG_GG Cuts at: 1348 3918 4088 1348 Size: 2570 170 6270 Fragments arranged by size: 6270 2570 170 PciI A'CATG_T Cuts at: 7909 7909 Size: 9010 PflMI CCAn_nnn'nTGG Cuts at: 1108 4082 4094 1108 Size: 2974 12 6024 Fragments arranged by size: 6024 2974 12 PfoI T'CCnGG_A Cuts at: 2890 4075 4105 2890 Size: 1185 30 7795 Fragments arranged by size: 7795 1185 30 PmeI GTTT'AAAC Cuts at: 6314 6314 Size: 9010 PmlI CAC'GTG Cuts at: 3761 3761 Size: 9010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7079 7111 8592 8624 7079 Size: 32 1481 32 7465 Fragments arranged by size: 7465 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2037 4079 4514 4583 5424 5642 2037 Size: 2042 435 69 841 218 5405 Fragments arranged by size: 5405 2042 841 435 218 69 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9010 PsiI TTA'TAA Cuts at: 3540 6580 3540 Size: 3040 5970 PspOMI G'GGCC_C Cuts at: 4180 4472 4180 Size: 292 8718 PspXI vC'TCGA_Gb Cuts at: 4753 4753 Size: 9010 PstI C_TGCA'G Cuts at: 830 4051 5899 6362 830 Size: 3221 1848 463 3478 Fragments arranged by size: 3478 3221 1848 463 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9010 PvuII CAG'CTG Cuts at: 3221 4025 4043 4556 4870 5062 5293 3221 Size: 804 18 513 314 192 231 6938 Fragments arranged by size: 6938 804 513 314 231 192 18 SacI G_AGCT'C Cuts at: 4575 4575 Size: 9010 SalI G'TCGA_C Cuts at: 1936 6352 1936 Size: 4416 4594 SbfI CC_TGCA'GG Cuts at: 6362 6362 Size: 9010 ScaI AGT'ACT Cuts at: 5560 7258 8970 5560 Size: 1698 1712 5600 Fragments arranged by size: 5600 1712 1698 SexAI A'CCwGG_T Cuts at: 6040 6040 Size: 9010 SfcI C'TryA_G Cuts at: 826 1045 4047 4322 4725 5895 5983 6358 7493 Size: 219 3002 275 403 1170 88 375 1135 Cuts at: 7493 8174 8365 826 Size: 681 191 1471 Fragments arranged by size: 3002 1471 1170 1135 681 403 375 275 219 191 88 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9010 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9010 SmaI CCC'GGG Cuts at: 1872 6337 1872 Size: 4465 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3701 3860 4753 7114 8015 8277 Size: 197 926 1758 159 893 2361 901 262 Cuts at: 8277 820 Size: 1553 Fragments arranged by size: 2361 1758 1553 926 901 893 262 197 159 SnaBI TAC'GTA Cuts at: 493 6801 493 Size: 6308 2702 SpeI A'CTAG_T Cuts at: 152 3944 152 Size: 3792 5218 SphI G_CATG'C Cuts at: 1132 2169 4371 6368 1132 Size: 1037 2202 1997 3774 Fragments arranged by size: 3774 2202 1997 1037 SspI AAT'ATT Cuts at: 5 52 5386 6934 5 Size: 47 5334 1548 2081 Fragments arranged by size: 5334 2081 1548 47 StuI AGG'CCT Cuts at: 1517 3254 1517 Size: 1737 7273 StyI C'CwwG_G Cuts at: 513 1065 3013 3107 3352 4893 5102 6136 6449 Size: 552 1948 94 245 1541 209 1034 313 Cuts at: 6449 513 Size: 3074 Fragments arranged by size: 3074 1948 1541 1034 552 313 245 209 94 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2101 2458 3737 5924 5981 7037 7054 Size: 1255 521 357 1279 2187 57 1056 17 Cuts at: 7054 7207 7392 7837 325 Size: 153 185 445 1498 Fragments arranged by size: 2187 1498 1279 1255 1056 521 445 357 185 153 57 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5558 7256 8968 Size: 274 80 33 51 1443 3581 1698 1712 Cuts at: 8968 96 Size: 138 Fragments arranged by size: 3581 1712 1698 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2177 2209 8970 9002 2177 Size: 32 6761 32 2185 Fragments arranged by size: 6761 2185 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4101 4160 1316 Size: 2785 59 6166 Fragments arranged by size: 6166 2785 59 XbaI T'CTAG_A Cuts at: 6346 6346 Size: 9010 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4192 5359 4192 Size: 1167 7843 XhoI C'TCGA_G Cuts at: 1943 4753 1943 Size: 2810 6200 XmaI C'CCGG_G Cuts at: 1870 6335 1870 Size: 4465 4545 XmnI GAAnn'nnTTC Cuts at: 2106 2750 4698 7139 2106 Size: 644 1948 2441 3977 Fragments arranged by size: 3977 2441 1948 644 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6818 276 Size: 53 83 186 936 5284 2468 Fragments arranged by size: 5284 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI