Restriction map for
pFN21AA1685
(Linear) (Six-base) MAPSORT of: KIAA1685.seq Check: 9432 from: 1 to: 4305
KIAA1685.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 377 1047 2355 2736 4305
Size: 377 670 1308 381 1569
Fragments arranged by size:
1569 1308 670 381 377
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 1364 2588 3091 3184 3458 3488 4305
Size: 983 381 1224 503 93 274 30 817
Fragments arranged by size:
1224 983 817 503 381 274 93 30
AfeI AGC'GCT
Cuts at: 0 2975 4305
Size: 2975 1330
AflIII A'CryG_T
Cuts at: 0 1755 4305
Size: 1755 2550
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3085 4305
Size: 3085 1220
AlwNI CAG_nnn'CTG
Cuts at: 0 35 2020 2041 2077 2200 2512 2641 2728
Size: 35 1985 21 36 123 312 129 87
Cuts at: 2728 3057 4087 4305
Size: 329 1030 218
Fragments arranged by size:
1985 1030 329 312 218 129 123 87
36 35 21
ApaI G_GGCC'C
Cuts at: 0 2179 2471 4305
Size: 2179 292 1834
Fragments arranged by size:
2179 1834 292
ApoI r'AATT_y
Cuts at: 0 98 988 1064 2897 4305
Size: 98 890 76 1833 1408
Fragments arranged by size:
1833 1408 890 98 76
AvaI C'yCGr_G
Cuts at: 0 2748 3856 4305
Size: 2748 1108 449
Fragments arranged by size:
2748 1108 449
BanI G'GyrC_C
Cuts at: 0 2527 4305
Size: 2527 1778
BanII G_rGCy'C
Cuts at: 0 1388 1687 1920 2179 2471 2570 3403 3596
Size: 1388 299 233 259 292 99 833 193
Cuts at: 3596 4305
Size: 709
Fragments arranged by size:
1388 833 709 299 292 259 233 193
99
BbsI GAAGACnn'nnnn_
Cuts at: 0 1700 3299 4305
Size: 1700 1599 1006
Fragments arranged by size:
1700 1599 1006
BbvCI CC'TCA_GC
Cuts at: 0 1398 3130 4305
Size: 1398 1732 1175
Fragments arranged by size:
1732 1398 1175
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2739 2773 4305
Size: 2739 34 1532
Fragments arranged by size:
2739 1532 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2845 4305
Size: 2845 1460
BclI T'GATC_A
Cuts at: 0 857 1677 3989 4220 4305
Size: 857 820 2312 231 85
Fragments arranged by size:
2312 857 820 231 85
BglI GCCn_nnn'nGGC
Cuts at: 0 2443 4305
Size: 2443 1862
BglII A'GATC_T
Cuts at: 0 754 4305
Size: 754 3551
BlpI GC'TnA_GC
Cuts at: 0 972 1420 3675 4305
Size: 972 448 2255 630
Fragments arranged by size:
2255 972 630 448
Bme1580I G_kGCm'C
Cuts at: 0 1447 2179 2471 3368 4186 4305
Size: 1447 732 292 897 818 119
Fragments arranged by size:
1447 897 818 732 292 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 1181 1275 2250 2326 2465 4305
Size: 1181 94 975 76 139 1840
Fragments arranged by size:
1840 1181 975 139 94 76
BmtI G_CTAG'C
Cuts at: 0 3683 4109 4305
Size: 3683 426 196
Fragments arranged by size:
3683 426 196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 672 908 1854 2327 3123 4305
Size: 174 498 236 946 473 796 1182
Fragments arranged by size:
1182 946 796 498 473 236 174
Bpu10I CC'TnA_GC
Cuts at: 0 1150 1398 3122 3130 4305
Size: 1150 248 1724 8 1175
Fragments arranged by size:
1724 1175 1150 248 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1681 1840 4305
Size: 1681 159 2465
Fragments arranged by size:
2465 1681 159
BsaI GGTCTCn'nnnn_
Cuts at: 0 1800 1872 1911 2257 4305
Size: 1800 72 39 346 2048
Fragments arranged by size:
2048 1800 346 72 39
BsaAI yAC'GTr
Cuts at: 0 1756 4305
Size: 1756 2549
BsaBI GATnn'nnATC
Cuts at: 0 1457 4305
Size: 1457 2848
BsaWI w'CCGG_w
Cuts at: 0 819 4305
Size: 819 3486
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 172 202 670 700 1167 1197 4305
Size: 172 30 468 30 467 30 3108
Fragments arranged by size:
3108 468 467 172 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 143 555 2147 3604 4305
Size: 143 412 1592 1457 701
Fragments arranged by size:
1592 1457 701 412 143
BseYI C'CCAG_C
Cuts at: 0 1212 1739 3057 3321 4123 4305
Size: 1212 527 1318 264 802 182
Fragments arranged by size:
1318 1212 802 527 264 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1378 2567 4305
Size: 1378 1189 1738
Fragments arranged by size:
1738 1378 1189
BsiEI CG_ry'CG
Cuts at: 0 3396 4305
Size: 3396 909
BsiHKAI G_wGCw'C
Cuts at: 0 855 2313 2570 2755 4305
Size: 855 1458 257 185 1550
Fragments arranged by size:
1550 1458 855 257 185
BsmI GAATG_Cn'
Cuts at: 0 174 1427 4305
Size: 174 1253 2878
Fragments arranged by size:
2878 1253 174
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4290 4305
Size: 4290 15
Bsp1286I G_dGCh'C
Cuts at: 0 855 1388 1447 1687 1920 2179 2313 2471
Size: 855 533 59 240 233 259 134 158
Cuts at: 2471 2570 2755 3368 3403 3596 4186 4305
Size: 99 185 613 35 193 590 119
Fragments arranged by size:
855 613 590 533 259 240 233 193
185 158 134 119 99 59 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 377 442 627 636 1047 1893 2355 2566
Size: 377 65 185 9 411 846 462 211
Cuts at: 2566 2736 3360 4305
Size: 170 624 945
Fragments arranged by size:
945 846 624 462 411 377 211 185
170 65 9
BsrBI CCG'CTC
Cuts at: 0 1630 4305
Size: 1630 2675
BsrDI GCAATG_nn'
Cuts at: 0 179 547 1303 3362 4062 4305
Size: 179 368 756 2059 700 243
Fragments arranged by size:
2059 756 700 368 243 179
BstAPI GCAn_nnn'nTGC
Cuts at: 0 906 2020 2041 2901 4305
Size: 906 1114 21 860 1404
Fragments arranged by size:
1404 1114 906 860 21
BstBI TT'CG_AA
Cuts at: 0 4192 4305
Size: 4192 113
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3613 4305
Size: 3613 692
BstYI r'GATC_y
Cuts at: 0 754 1458 1859 3194 4305
Size: 754 704 401 1335 1111
Fragments arranged by size:
1335 1111 754 704 401
BtgI C'CryG_G
Cuts at: 0 1102 2888 4131 4305
Size: 1102 1786 1243 174
Fragments arranged by size:
1786 1243 1102 174
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1553 1883 4305
Size: 1553 330 2422
Fragments arranged by size:
2422 1553 330
BtsI GCAGTG_nn'
Cuts at: 0 309 357 3833 4305
Size: 309 48 3476 472
Fragments arranged by size:
3476 472 309 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4218 4253 4305
Size: 4218 35 52
Fragments arranged by size:
4218 52 35
DraI TTT'AAA
Cuts at: 0 2798 4305
Size: 2798 1507
DrdI GACnn_nn'nnGTC
Cuts at: 0 941 4305
Size: 941 3364
EaeI y'GGCC_r
Cuts at: 0 3393 4134 4305
Size: 3393 741 171
Fragments arranged by size:
3393 741 171
EagI C'GGCC_G
Cuts at: 0 3393 4305
Size: 3393 912
EarI CTCTTCn'nnn_
Cuts at: 0 194 1328 1718 1741 4160 4305
Size: 194 1134 390 23 2419 145
Fragments arranged by size:
2419 1134 390 194 145 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 55 4305
Size: 55 4250
EcoICRI GAG'CTC
Cuts at: 0 2568 4305
Size: 2568 1737
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 174 672 908 983 1364 1854 2327 2588
Size: 174 498 236 75 381 490 473 261
Cuts at: 2588 3091 3123 3184 3458 3488 4305
Size: 503 32 61 274 30 817
Fragments arranged by size:
817 503 498 490 473 381 274 261
236 174 75 61 32 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2081 4305
Size: 2081 2224
EcoO109I rG'GnC_Cy
Cuts at: 0 32 1728 2074 2175 2176 2467 2509 2578
Size: 32 1696 346 101 1 291 42 69
Cuts at: 2578 3101 3419 3637 4305
Size: 523 318 218 668
Fragments arranged by size:
1696 668 523 346 318 291 218 101
69 42 32 1
EcoRI G'AATT_C
Cuts at: 0 988 1064 4305
Size: 988 76 3241
Fragments arranged by size:
3241 988 76
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 75 118 164 4305
Size: 75 43 46 4141
Fragments arranged by size:
4141 75 46 43
HaeII r_GCGC'y
Cuts at: 0 508 2977 4244 4305
Size: 508 2469 1267 61
Fragments arranged by size:
2469 1267 508 61
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 138 170 298 330 842 874 1167 1199
Size: 138 32 128 32 512 32 293 32
Cuts at: 1199 3016 3048 3216 3248 3556 3588 3974 4006
Size: 1817 32 168 32 308 32 386 32
Cuts at: 4006 4009 4041 4057 4089 4305
Size: 3 32 16 32 216
Fragments arranged by size:
1817 512 386 308 293 216 168 138
128 32 32 32 32 32 32 32
32 32 32 16 3
HindIII A'AGCT_T
Cuts at: 0 2692 4305
Size: 2692 1613
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 2037 2365 2527 4305
Size: 593 1444 328 162 1778
Fragments arranged by size:
1778 1444 593 328 162
MscI TGG'CCA
Cuts at: 0 4136 4305
Size: 4136 169
MslI CAynn'nnrTG
Cuts at: 0 1237 4222 4305
Size: 1237 2985 83
Fragments arranged by size:
2985 1237 83
MspA1I CmG'CkG
Cuts at: 0 1216 2020 2026 2038 2551 2865 3057 3288
Size: 1216 804 6 12 513 314 192 231
Cuts at: 3288 4305
Size: 1017
Fragments arranged by size:
1216 1017 804 513 314 231 192 12
6
NcoI C'CATG_G
Cuts at: 0 1102 2888 4131 4305
Size: 1102 1786 1243 174
Fragments arranged by size:
1786 1243 1102 174
NheI G'CTAG_C
Cuts at: 0 3679 4105 4305
Size: 3679 426 200
Fragments arranged by size:
3679 426 200
NspI r_CATG'y
Cuts at: 0 164 2366 4305
Size: 164 2202 1939
Fragments arranged by size:
2202 1939 164
PasI CC'CwG_GG
Cuts at: 0 1913 2083 4305
Size: 1913 170 2222
Fragments arranged by size:
2222 1913 170
PflMI CCAn_nnn'nTGG
Cuts at: 0 2077 2089 4305
Size: 2077 12 2216
Fragments arranged by size:
2216 2077 12
PfoI T'CCnGG_A
Cuts at: 0 885 2070 2100 4305
Size: 885 1185 30 2205
Fragments arranged by size:
2205 1185 885 30
PmlI CAC'GTG
Cuts at: 0 1756 4305
Size: 1756 2549
PpuMI rG'GwC_Cy
Cuts at: 0 32 2074 2509 2578 3419 3637 4305
Size: 32 2042 435 69 841 218 668
Fragments arranged by size:
2042 841 668 435 218 69 32
PsiI TTA'TAA
Cuts at: 0 1535 4305
Size: 1535 2770
PspOMI G'GGCC_C
Cuts at: 0 2175 2467 4305
Size: 2175 292 1838
Fragments arranged by size:
2175 1838 292
PspXI vC'TCGA_Gb
Cuts at: 0 2748 4305
Size: 2748 1557
PstI C_TGCA'G
Cuts at: 0 2046 3894 4305
Size: 2046 1848 411
Fragments arranged by size:
2046 1848 411
PvuII CAG'CTG
Cuts at: 0 1216 2020 2038 2551 2865 3057 3288 4305
Size: 1216 804 18 513 314 192 231 1017
Fragments arranged by size:
1216 1017 804 513 314 231 192 18
SacI G_AGCT'C
Cuts at: 0 2570 4305
Size: 2570 1735
ScaI AGT'ACT
Cuts at: 0 3555 4305
Size: 3555 750
SexAI A'CCwGG_T
Cuts at: 0 4035 4305
Size: 4035 270
SfcI C'TryA_G
Cuts at: 0 2042 2317 2720 3890 3978 4305
Size: 2042 275 403 1170 88 327
Fragments arranged by size:
2042 1170 403 327 275 88
SmlI C'TyrA_G
Cuts at: 0 1696 1855 2748 4305
Size: 1696 159 893 1557
Fragments arranged by size:
1696 1557 893 159
SpeI A'CTAG_T
Cuts at: 0 1939 4305
Size: 1939 2366
SphI G_CATG'C
Cuts at: 0 164 2366 4305
Size: 164 2202 1939
Fragments arranged by size:
2202 1939 164
SspI AAT'ATT
Cuts at: 0 3381 4305
Size: 3381 924
StuI AGG'CCT
Cuts at: 0 1249 4305
Size: 1249 3056
StyI C'CwwG_G
Cuts at: 0 1008 1102 1347 2888 3097 4131 4305
Size: 1008 94 245 1541 209 1034 174
Fragments arranged by size:
1541 1034 1008 245 209 174 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 96 453 1732 3919 3976 4305
Size: 96 357 1279 2187 57 329
Fragments arranged by size:
2187 1279 357 329 96 57
TatI w'GTAC_w
Cuts at: 0 3553 4305
Size: 3553 752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 172 204 4305
Size: 172 32 4101
Fragments arranged by size:
4101 172 32
Tth111I GACn'n_nGTC
Cuts at: 0 2096 2155 4305
Size: 2096 59 2150
Fragments arranged by size:
2150 2096 59
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2187 3354 4305
Size: 2187 1167 951
Fragments arranged by size:
2187 1167 951
XhoI C'TCGA_G
Cuts at: 0 2748 4305
Size: 2748 1557
XmnI GAAnn'nnTTC
Cuts at: 0 101 745 2693 4305
Size: 101 644 1948 1612
Fragments arranged by size:
1948 1612 644 101
Enzymes that do cut:
AarI AcuI AfeI AflIII AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HindIII
MmeI MscI MslI MspA1I NcoI NheI NspI PasI
PflMI PfoI PmlI PpuMI PsiI PspOMI PspXI PstI
PvuII SacI ScaI SexAI SfcI SmlI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XcmI
XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BfrBI BmgBI BplI BsaHI BsiWI BspEI BspHI
BsrFI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I ClaI
DraIII EcoRV FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PciI PmeI PpiI PshAI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SrfI SwaI XbaI
XmaI ZraI