(Circular) (Six-base) MAPSORT of: pFN21AA1664.seq Check: 8117 from: 1 to: 7453 pFN21AA1664 7453 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3699 3862 3699 Size: 163 7290 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2267 5263 278 Size: 53 83 186 936 731 2996 2468 Fragments arranged by size: 2996 2468 936 731 186 83 53 AccI GT'mk_AC Cuts at: 1937 4796 1937 Size: 2859 4594 Acc65I G'GTAC_C Cuts at: 4774 4774 Size: 7453 AclI AA'CG_TT Cuts at: 1563 5580 5953 1563 Size: 4017 373 3063 Fragments arranged by size: 4017 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2612 2942 2957 3074 3453 4169 5516 6900 Size: 980 330 15 117 379 716 1347 1384 Cuts at: 6900 7168 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1347 980 716 379 330 268 117 15 AfeI AGC'GCT Cuts at: 2125 2999 2125 Size: 874 6579 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7256 AflIII A'CryG_T Cuts at: 1203 2670 3906 4035 4395 6352 7329 1203 Size: 1467 1236 129 360 1957 977 1327 Fragments arranged by size: 1957 1467 1327 1236 977 360 129 AgeI A'CCGG_T Cuts at: 4137 6284 4137 Size: 2147 5306 AhdI GACnn_n'nnGTC Cuts at: 6182 6182 Size: 7453 AleI CACnn'nnGTG Cuts at: 1166 3776 1166 Size: 2610 4843 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2648 3727 1184 Size: 1464 1079 4910 Fragments arranged by size: 4910 1464 1079 AlwNI CAG_nnn'CTG Cuts at: 2285 3664 4342 4486 4534 4552 4596 5196 6768 Size: 1379 678 144 48 18 44 600 1572 Cuts at: 6768 2285 Size: 2970 Fragments arranged by size: 2970 1572 1379 678 600 144 48 44 18 ApaI G_GGCC'C Cuts at: 2358 2358 Size: 7453 ApaLI G'TGCA_C Cuts at: 3194 3707 3779 5510 6666 3194 Size: 513 72 1731 1156 3981 Fragments arranged by size: 3981 1731 1156 513 72 ApoI r'AATT_y Cuts at: 1493 4010 4762 4988 5140 7249 1493 Size: 2517 752 226 152 2109 1697 Fragments arranged by size: 2517 2109 1697 752 226 152 AseI AT'TA_AT Cuts at: 160 1032 6007 160 Size: 872 4975 1606 Fragments arranged by size: 4975 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7453 AvaI C'yCGr_G Cuts at: 1870 1943 2388 2420 3242 4778 1870 Size: 73 445 32 822 1536 4545 Fragments arranged by size: 4545 1536 822 445 73 32 BamHI G'GATC_C Cuts at: 3615 4660 4783 5150 3615 Size: 1045 123 367 5918 Fragments arranged by size: 5918 1045 367 123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2110 2984 3491 3995 4140 Size: 325 217 627 323 874 507 504 145 Cuts at: 4140 4560 4774 6229 618 Size: 420 214 1455 1842 Fragments arranged by size: 1842 1455 874 627 507 504 420 325 323 217 214 145 BanII G_rGCy'C Cuts at: 1391 2064 2154 2358 3470 3609 3645 4772 1391 Size: 673 90 204 1112 139 36 1127 4072 Fragments arranged by size: 4072 1127 1112 673 204 139 90 36 BbeI G_GCGC'C Cuts at: 2114 2988 4564 2114 Size: 874 1576 5003 Fragments arranged by size: 5003 1576 874 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2486 3381 3423 4331 928 Size: 971 587 895 42 908 4050 Fragments arranged by size: 4050 971 908 895 587 42 BbvCI CC'TCA_GC Cuts at: 2951 3892 2951 Size: 941 6512 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5644 5678 1903 Size: 34 3707 34 3678 Fragments arranged by size: 3707 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5345 6555 5345 Size: 1210 6243 BclI T'GATC_A Cuts at: 1548 3105 3399 1548 Size: 1557 294 5602 Fragments arranged by size: 5602 1557 294 BfrBI ATG'CAT Cuts at: 4285 4285 Size: 7453 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2034 2534 6064 Size: 107 122 71 631 605 362 500 3530 Cuts at: 6064 136 Size: 1525 Fragments arranged by size: 3530 1525 631 605 500 362 122 107 71 BglII A'GATC_T Cuts at: 7448 7448 Size: 7453 BlpI GC'TnA_GC Cuts at: 3469 4870 3469 Size: 1401 6052 Bme1580I G_kGCm'C Cuts at: 1790 2178 2358 2530 3169 3198 3265 3507 3711 Size: 388 180 172 639 29 67 242 204 Cuts at: 3711 3783 4742 5514 6670 1790 Size: 72 959 772 1156 2573 Fragments arranged by size: 2573 1156 959 772 639 388 242 204 180 172 72 67 29 BmgBI CAC'GTC Cuts at: 1318 2133 1318 Size: 815 6638 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4714 5184 6142 Size: 416 53 83 399 24 3297 470 958 Cuts at: 6142 442 Size: 1753 Fragments arranged by size: 3297 1753 958 470 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7453 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2353 2401 2353 Size: 48 7405 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2506 2831 2978 3484 3595 3641 3698 3937 Size: 1267 325 147 506 111 46 57 239 Cuts at: 3937 3946 4556 4592 6113 1239 Size: 9 610 36 1521 2579 Fragments arranged by size: 2579 1521 1267 610 506 325 239 147 111 57 46 36 9 Bpu10I CC'TnA_GC Cuts at: 2338 2951 3892 2338 Size: 613 941 5899 Fragments arranged by size: 5899 941 613 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2558 2750 3008 5578 6443 6741 2558 Size: 192 258 2570 865 298 3270 Fragments arranged by size: 3270 2570 865 298 258 192 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3812 6116 882 Size: 617 2313 2304 2219 Fragments arranged by size: 2313 2304 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2673 2958 3907 4036 4237 5244 493 Size: 711 1469 285 949 129 201 1007 2702 Fragments arranged by size: 2702 1469 1007 949 711 285 201 129 BsaBI GATnn'nnATC Cuts at: 3947 7447 3947 Size: 3500 3953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2111 2264 2985 4561 Size: 53 83 186 936 578 153 721 1576 Cuts at: 4561 5260 5642 6277 275 Size: 699 382 635 1451 Fragments arranged by size: 1576 1451 936 721 699 635 578 382 186 153 83 53 BsaWI w'CCGG_w Cuts at: 2182 4137 5886 6284 6558 6705 7116 2182 Size: 1955 1749 398 274 147 411 2519 Fragments arranged by size: 2519 1955 1749 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3719 3749 3896 3926 4382 Size: 30 165 30 2316 30 147 30 456 Cuts at: 4382 4412 1178 Size: 30 4219 Fragments arranged by size: 4219 2316 456 165 147 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2475 4454 4553 1186 Size: 1289 1979 99 4086 Fragments arranged by size: 4086 1979 1289 99 BseYI C'CCAG_C Cuts at: 2160 2166 2285 2351 2620 2890 3811 4295 4304 Size: 6 119 66 269 270 921 484 9 Cuts at: 4304 6656 7152 2160 Size: 2352 496 2461 Fragments arranged by size: 2461 2352 921 496 484 270 269 119 66 9 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2894 3697 1163 Size: 573 1158 803 4919 Fragments arranged by size: 4919 1158 803 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2050 2321 3275 3601 4903 5664 Size: 275 186 49 271 954 326 1302 761 Cuts at: 5664 6692 1540 Size: 1028 2301 Fragments arranged by size: 2301 1302 1028 954 761 326 275 271 186 49 BsiHKAI G_wGCw'C Cuts at: 3198 3470 3609 3711 3783 4052 4274 4437 5514 Size: 272 139 102 72 269 222 163 1077 Cuts at: 5514 5599 6670 3198 Size: 85 1071 3981 Fragments arranged by size: 3981 1077 1071 272 269 222 163 139 102 85 72 BsmI GAATG_Cn' Cuts at: 3540 4964 5057 3540 Size: 1424 93 5936 Fragments arranged by size: 5936 1424 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2252 2252 Size: 7453 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2154 2178 2358 2530 3169 3198 Size: 399 274 90 24 180 172 639 29 Cuts at: 3198 3265 3470 3507 3609 3645 3711 3783 4052 Size: 67 205 37 102 36 66 72 269 Cuts at: 4052 4274 4437 4742 4772 5514 5599 6670 1391 Size: 222 163 305 30 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 639 399 305 274 269 222 205 180 172 163 102 90 85 72 67 66 37 36 30 29 24 BspEI T'CCGG_A Cuts at: 7116 7116 Size: 7453 BspHI T'CATG_A Cuts at: 3582 5342 7072 3582 Size: 1760 1730 3963 Fragments arranged by size: 3963 1760 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3699 3862 4808 844 Size: 2855 163 946 3489 Fragments arranged by size: 3489 2855 946 163 BsrBI CCG'CTC Cuts at: 1394 1823 2052 2188 2383 3070 3247 5340 1394 Size: 429 229 136 195 687 177 2093 3507 Fragments arranged by size: 3507 2093 687 429 229 195 177 136 BsrDI GCAATG_nn' Cuts at: 65 1248 2632 2968 5948 6122 65 Size: 1183 1384 336 2980 174 1396 Fragments arranged by size: 2980 1396 1384 1183 336 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2787 3270 3274 3625 4137 4563 6097 Size: 110 977 483 4 351 512 426 1534 Cuts at: 6097 6284 1700 Size: 187 2869 Fragments arranged by size: 2869 1534 977 512 483 426 351 187 110 4 BsrGI T'GTAC_A Cuts at: 96 4689 96 Size: 4593 2860 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7453 BssSI C'ACGA_G Cuts at: 3500 4249 4436 5513 6525 3500 Size: 749 187 1077 1012 4428 Fragments arranged by size: 4428 1077 1012 749 187 BstAPI GCAn_nnn'nTGC Cuts at: 794 3193 4596 794 Size: 2399 1403 3651 Fragments arranged by size: 3651 2399 1403 BstBI TT'CG_AA Cuts at: 6257 6257 Size: 7453 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7453 BstXI CCAn_nnnn'nTGG Cuts at: 3724 4022 3724 Size: 298 7155 BstYI r'GATC_y Cuts at: 1972 3027 3615 4660 4783 5150 5535 5552 7004 Size: 1055 588 1045 123 367 385 17 1452 Cuts at: 7004 7090 7102 7448 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1055 1045 588 385 367 346 123 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2056 2677 3369 3456 513 Size: 552 939 52 621 692 87 4510 Fragments arranged by size: 4510 939 692 621 552 87 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2956 3772 487 Size: 683 56 1730 816 4168 Fragments arranged by size: 4168 1730 816 683 56 BtsI GCAGTG_nn' Cuts at: 2238 2801 3167 3709 3711 4969 5763 5783 2238 Size: 563 366 542 2 1258 794 20 3908 Fragments arranged by size: 3908 1258 794 563 542 366 20 2 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7453 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3438 3473 529 Size: 35 2874 35 4509 Fragments arranged by size: 4509 2874 35 35 DraI TTT'AAA Cuts at: 4757 4757 Size: 7453 DraIII CAC_nnn'GTG Cuts at: 3907 4036 3907 Size: 129 7324 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6460 809 Size: 507 5144 1802 Fragments arranged by size: 5144 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2047 2318 2817 Size: 54 1425 50 275 18 217 271 499 Cuts at: 2817 3268 3272 3276 3598 3744 4900 5789 7418 Size: 451 4 4 322 146 1156 889 1629 Cuts at: 7418 8 Size: 43 Fragments arranged by size: 1629 1425 1156 889 499 451 322 275 271 217 146 54 50 43 18 4 4 EagI C'GGCC_G Cuts at: 1537 1812 2047 2318 3272 3598 4900 1537 Size: 275 235 271 954 326 1302 4090 Fragments arranged by size: 4090 1302 954 326 275 271 235 EarI CTCTTCn'nnn_ Cuts at: 1353 2463 3086 4040 4605 4646 5383 1353 Size: 1110 623 954 565 41 737 3423 Fragments arranged by size: 3423 1110 954 737 623 565 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4637 6040 6414 6560 387 Size: 1064 138 3048 1403 374 146 1280 Fragments arranged by size: 3048 1403 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3468 3607 3468 Size: 139 7314 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2506 2612 2831 2942 2957 2978 3074 Size: 393 874 106 219 111 15 21 96 Cuts at: 3074 3453 3484 3595 3641 3698 3937 3946 4169 Size: 379 31 111 46 57 239 9 223 Cuts at: 4169 4556 4592 5516 6113 6900 7168 1239 Size: 387 36 924 597 787 268 1524 Fragments arranged by size: 1524 924 874 787 597 393 387 379 268 239 223 219 111 111 106 96 57 46 36 31 21 15 9 EcoNI CCTnn'n_nnAGG Cuts at: 3935 3935 Size: 7453 EcoO109I rG'GnC_Cy Cuts at: 2355 3673 2355 Size: 1318 6135 EcoRI G'AATT_C Cuts at: 4010 4762 5140 4010 Size: 752 378 6323 Fragments arranged by size: 6323 752 378 FseI GG_CCGG'CC Cuts at: 3274 3278 3274 Size: 4 7449 FspI TGC'GCA Cuts at: 3900 5959 3900 Size: 2059 5394 HaeII r_GCGC'y Cuts at: 1678 2114 2127 2608 2988 3001 4454 4552 4564 Size: 436 13 481 380 13 1453 98 12 Cuts at: 4564 6600 1678 Size: 2036 2531 Fragments arranged by size: 2531 2036 1453 481 436 380 98 13 13 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2249 2281 2414 2446 2572 Size: 32 135 32 677 32 133 32 126 Cuts at: 2572 2604 2833 2865 3133 3165 3368 3400 3452 Size: 32 229 32 268 32 203 32 52 Cuts at: 3452 3479 3484 3511 3632 3664 4645 4677 6094 Size: 27 5 27 121 32 981 32 1417 Cuts at: 6094 6126 6168 6200 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1417 981 677 268 229 203 135 133 126 121 52 42 32 32 32 32 32 32 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 4797 5043 669 Size: 988 281 2859 246 3079 Fragments arranged by size: 3079 2859 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7453 HpaI GTT'AAC Cuts at: 5043 5043 Size: 7453 KasI G'GCGC_C Cuts at: 2110 2984 4560 2110 Size: 874 1576 5003 Fragments arranged by size: 5003 1576 874 KpnI G_GTAC'C Cuts at: 4778 4778 Size: 7453 MfeI C'AATT_G Cuts at: 5052 5052 Size: 7453 MluI A'CGCG_T Cuts at: 7329 7329 Size: 7453 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2193 3131 6567 6751 1305 Size: 888 938 3436 184 2007 Fragments arranged by size: 3436 2007 938 888 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2819 10 Size: 54 1425 343 987 4644 Fragments arranged by size: 4644 1425 987 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2856 3395 3626 3776 4073 5411 5770 Size: 648 1690 539 231 150 297 1338 359 Cuts at: 5770 5929 518 Size: 159 2042 Fragments arranged by size: 2042 1690 1338 648 539 359 297 231 159 150 MspA1I CmG'CkG Cuts at: 1606 2058 2197 2610 3163 3220 3661 3896 4147 Size: 452 139 413 553 57 441 235 251 Cuts at: 4147 4375 4483 4531 4596 4666 4869 5546 6694 Size: 228 108 48 65 70 203 677 1148 Cuts at: 6694 6939 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 553 452 441 413 251 245 235 228 203 139 108 70 65 57 48 NaeI GCC'GGC Cuts at: 3272 3276 4565 3272 Size: 4 1289 6160 Fragments arranged by size: 6160 1289 4 NarI GG'CG_CC Cuts at: 2111 2985 4561 2111 Size: 874 1576 5003 Fragments arranged by size: 5003 1576 874 NcoI C'CATG_G Cuts at: 513 1065 2004 3369 513 Size: 552 939 1365 4597 Fragments arranged by size: 4597 1365 939 552 NdeI CA'TA_TG Cuts at: 387 4734 387 Size: 4347 3106 NgoMIV G'CCGG_C Cuts at: 3270 3274 4563 3270 Size: 4 1289 6160 Fragments arranged by size: 6160 1289 4 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7453 NotI GC'GGCC_GC Cuts at: 2047 4900 2047 Size: 2853 4600 NsiI A_TGCA'T Cuts at: 4287 4287 Size: 7453 NspI r_CATG'y Cuts at: 1132 1229 4399 4811 6356 1132 Size: 97 3170 412 1545 2229 Fragments arranged by size: 3170 2229 1545 412 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7453 PciI A'CATG_T Cuts at: 4395 6352 4395 Size: 1957 5496 PflMI CCAn_nnn'nTGG Cuts at: 1108 2285 3694 1108 Size: 1177 1409 4867 Fragments arranged by size: 4867 1409 1177 PfoI T'CCnGG_A Cuts at: 3611 3611 Size: 7453 PmeI GTTT'AAAC Cuts at: 4757 4757 Size: 7453 PmlI CAC'GTG Cuts at: 2673 2958 2673 Size: 285 7168 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5522 5554 7035 7067 5522 Size: 32 1481 32 5908 Fragments arranged by size: 5908 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3673 3673 Size: 7453 PshAI GACnn'nnGTC Cuts at: 1613 2197 2422 1613 Size: 584 225 6644 Fragments arranged by size: 6644 584 225 PsiI TTA'TAA Cuts at: 5023 5023 Size: 7453 PspOMI G'GGCC_C Cuts at: 2354 2354 Size: 7453 PspXI vC'TCGA_Gb Cuts at: 3242 3242 Size: 7453 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4385 4417 4385 Size: 32 7421 PstI C_TGCA'G Cuts at: 830 2310 3119 3174 4601 4805 830 Size: 1480 809 55 1427 204 3478 Fragments arranged by size: 3478 1480 1427 809 204 55 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7453 PvuII CAG'CTG Cuts at: 3220 3896 4147 4596 3220 Size: 676 251 449 6077 Fragments arranged by size: 6077 676 449 251 SacI G_AGCT'C Cuts at: 3470 3609 3470 Size: 139 7314 SacII CC_GC'GG Cuts at: 2059 2059 Size: 7453 SalI G'TCGA_C Cuts at: 1936 4795 1936 Size: 2859 4594 SapI GCTCTTCn'nnn_ Cuts at: 4040 4040 Size: 7453 SbfI CC_TGCA'GG Cuts at: 3119 4805 3119 Size: 1686 5767 ScaI AGT'ACT Cuts at: 3359 5701 7413 3359 Size: 2342 1712 3399 Fragments arranged by size: 3399 2342 1712 SfcI C'TryA_G Cuts at: 826 1045 2306 2644 3115 3170 3559 4162 4597 Size: 219 1261 338 471 55 389 603 435 Cuts at: 4597 4801 5936 6617 6808 826 Size: 204 1135 681 191 1471 Fragments arranged by size: 1471 1261 1135 681 603 471 435 389 338 219 204 191 55 SfoI GGC'GCC Cuts at: 2112 2986 4562 2112 Size: 874 1576 5003 Fragments arranged by size: 5003 1576 874 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7453 SgrAI Cr'CCGG_yG Cuts at: 1700 4563 1700 Size: 2863 4590 SmaI CCC'GGG Cuts at: 1872 2390 4780 1872 Size: 518 2390 4545 Fragments arranged by size: 4545 2390 518 SmlI C'TyrA_G Cuts at: 820 1017 1943 2573 2765 3023 3242 5557 6458 Size: 197 926 630 192 258 219 2315 901 Cuts at: 6458 6720 820 Size: 262 1553 Fragments arranged by size: 2315 1553 926 901 630 262 258 219 197 192 SnaBI TAC'GTA Cuts at: 493 5244 493 Size: 4751 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7453 SphI G_CATG'C Cuts at: 1132 4811 1132 Size: 3679 3774 SspI AAT'ATT Cuts at: 5 52 5377 5 Size: 47 5325 2081 Fragments arranged by size: 5325 2081 47 StuI AGG'CCT Cuts at: 1517 4577 1517 Size: 3060 4393 StyI C'CwwG_G Cuts at: 513 1065 2004 2820 2826 3369 3411 3507 4275 Size: 552 939 816 6 543 42 96 768 Cuts at: 4275 4713 4892 513 Size: 438 179 3074 Fragments arranged by size: 3074 939 816 768 552 543 438 179 96 42 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5480 5497 5650 5835 6280 325 Size: 1255 3900 17 153 185 445 1498 Fragments arranged by size: 3900 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3357 3481 4398 Size: 274 80 33 51 1443 1380 124 917 Cuts at: 4398 4689 5699 7411 96 Size: 291 1010 1712 138 Fragments arranged by size: 1712 1443 1380 1010 917 291 274 138 124 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3719 3751 7413 7445 3719 Size: 32 3662 32 3727 Fragments arranged by size: 3727 3662 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2263 4342 1316 Size: 947 2079 4427 Fragments arranged by size: 4427 2079 947 XbaI T'CTAG_A Cuts at: 4789 4789 Size: 7453 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2530 2675 2779 3217 3263 2530 Size: 145 104 438 46 6720 Fragments arranged by size: 6720 438 145 104 46 XhoI C'TCGA_G Cuts at: 1943 3242 1943 Size: 1299 6154 XmaI C'CCGG_G Cuts at: 1870 2388 4778 1870 Size: 518 2390 4545 Fragments arranged by size: 4545 2390 518 XmnI GAAnn'nnTTC Cuts at: 5582 5582 Size: 7453 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2265 5261 276 Size: 53 83 186 936 731 2996 2468 Fragments arranged by size: 2996 2468 936 731 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BsiWI BstZ17I Bsu36I EcoRV FalI FspAI NruI PacI RsrII SanDI SexAI SfiI SrfI SwaI