Restriction map for
pFN21AA1649
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BpmI BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV FbaI FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI
PflFI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccII 37 CGCG CG^CG
AfaI 999 GTAC GT^AC
AflIII 755 ACATGT A^C[GA][CT]GT
AgeI 1007 ACCGGT A^CCGGT
ApaI 911 GGGCCC GGGCC^C
AspHI 923 GAGCTC G[AT]GC[AT]^C
BamHI 104 GGATCC G^GATCC
BsaWI 1007 ACCGGT [AT]^CCGG[AT]
BsiHKAI 923 GAGCTC G[AT]GC[AT]^C
BspLU11I 755 ACATGT A^CATGT
BsrFI 1007 ACCGGT [GA]^CCGG[CT]
BstUI 37 CGCG CG^CG
BstXI 627 CCACATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 104 GGATCC [GA]^GATC[CT]
CfoI 36 GCGC GCG^C
Cfr10I 1007 ACCGGT [GA]^CCGG[CT]
DrdI 750 GACCAACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1246 GAATTC G^AATTC
EcoT22I 701 ATGCAT ATGCA^T
HhaI 36 GCGC GCG^C
HinP1I 36 GCGC G^CGC
HincII 129 GTCAAC GT[CT]^[GA]AC
HindII 129 GTCAAC GT[CT]^[GA]AC
MflI 104 GGATCC [GA]^GATC[CT]
MseI 62 TTAA T^TAA
MspA1I 803 CAGCTG C[AC]G^C[GT]G
MvnI 37 CGCG CG^CG
NcoI 467 CCATGG C^CATGG
NsiI 701 ATGCAT ATGCA^T
NspI 755 ACATGT [GA]CATG^[CT]
PinAI 1007 ACCGGT A^CCGGT
PvuII 803 CAGCTG CAG^CTG
RsaI 999 GTAC GT^AC
SacI 923 GAGCTC GAGCT^C
StuI 114 AGGCCT AGG^CCT
Tru9I 62 TTAA T^TAA
XcmI 624 CCACCACATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 104 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 537 GAATTT [GA]^AATT[CT]
1246 GAATTC
AlwI 105 GATCC GGATC(4/5)
104 GGATC
AlwNI 710 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
803 CAGCTGCTG
AocI 608 CCTCAGG CC^T[ACGT]AGG
1214 CCTCAGG
ApoI 537 GAATTT [GA]^AATT[CT]
1246 GAATTC
AvaII 933 GGTCC G^G[AT]CC
1179 GGACC
BanII 911 GGGCCC G[GA]GC[CT]^C
923 GAGCTC
BlpI 639 GCTAAGC GC^T[ACGT]AGC
808 GCTGAGC
Bpu1102I 639 GCTAAGC GC^T[ACGT]AGC
808 GCTGAGC
BsgI 859 CTGCAC GTGCAG(16/14)
271 GTGCAG
BsmFI 409 GGGAC GGGAC(10/14)
759 GTCCC
BstBI 254 TTCGAA TT^CGAA
734 TTCGAA
Bsu36I 608 CCTCAGG CC^T[ACGT]AGG
1214 CCTCAGG
CelII 639 GCTAAGC GC^T[ACGT]AGC
808 GCTGAGC
DsaI 314 CCACGG C^C[GA][CT]GG
467 CCATGG
EarI 1204 CTCTTC CTCTTC(1/4)
369 GAAGAG
Eco81I 608 CCTCAGG CC^T[ACGT]AGG
1214 CCTCAGG
EcoT14I 467 CCATGG C^C[AT][AT]GG
665 CCAAGG
FauI 332 CCCGC CCCGC(4/6)
38 GCGGG
Ksp632I 1204 CTCTTC CTCTTC(1/4)
369 GAAGAG
NspV 254 TTCGAA TT^CGAA
734 TTCGAA
PaeR7I 95 CTCGAG C^TCGAG
866 CTCGAG
PflMI 400 CCACCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
624 CCACCACATGG
PleI 209 GAGTC GAGTC(4/5)
869 GAGTC
SfuI 254 TTCGAA TT^CGAA
734 TTCGAA
SmaI 221 CCCGGG CCC^GGG
350 CCCGGG
StyI 467 CCATGG C^C[AT][AT]GG
665 CCAAGG
TfiI 257 GAATC G^A[AT]TC
503 GAATC
Tsp509I 538 AATT ^AATT
1247 AATT
Van91I 400 CCACCCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
624 CCACCACATGG
XhoI 95 CTCGAG C^TCGAG
866 CTCGAG
XmaCI 221 CCCGGG C^CCGGG
350 CCCGGG
XmaI 221 CCCGGG C^CCGGG
350 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AvaI BanI BbvI
BcnI BmyI BsaJI BseRI BsiYI
BslI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI MaeIII MboI
MboII MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI TseI Tsp45I TspRI TthHB8I