(Circular) (Six-base) MAPSORT of: pFN21AA1649.seq Check: 1190 from: 1 to: 5968 pFN21AA1649 5968 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3778 278 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3311 1937 Size: 1374 4594 Acc65I G'GTAC_C Cuts at: 3289 3289 Size: 5968 AclI AA'CG_TT Cuts at: 1563 4095 4468 1563 Size: 2532 373 3063 Fragments arranged by size: 3063 2532 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2453 3221 4031 5415 5683 1632 Size: 821 768 810 1384 268 1917 Fragments arranged by size: 1917 1384 821 810 768 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5771 AflIII A'CryG_T Cuts at: 1203 2760 4867 5844 1203 Size: 1557 2107 977 1327 Fragments arranged by size: 2107 1557 1327 977 AgeI A'CCGG_T Cuts at: 3012 4799 3012 Size: 1787 4181 AhdI GACnn_n'nnGTC Cuts at: 4697 4697 Size: 5968 AleI CACnn'nnGTG Cuts at: 1166 2690 1166 Size: 1524 4444 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2265 1184 Size: 1081 4887 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3140 3172 3140 Size: 32 5936 AlwNI CAG_nnn'CTG Cuts at: 2720 2813 3711 5283 2720 Size: 93 898 1572 3405 Fragments arranged by size: 3405 1572 898 93 ApaI G_GGCC'C Cuts at: 2920 2920 Size: 5968 ApaLI G'TGCA_C Cuts at: 4025 5181 4025 Size: 1156 4812 ApoI r'AATT_y Cuts at: 1493 2542 3251 3277 3503 3655 5764 1493 Size: 1049 709 26 226 152 2109 1697 Fragments arranged by size: 2109 1697 1049 709 226 152 26 AseI AT'TA_AT Cuts at: 160 1032 4522 160 Size: 872 3490 1606 Fragments arranged by size: 3490 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 AvaI C'yCGr_G Cuts at: 1870 1943 2100 2226 2355 2871 3293 1870 Size: 73 157 126 129 516 422 4545 Fragments arranged by size: 4545 516 422 157 129 126 73 BamHI G'GATC_C Cuts at: 2109 3298 3665 2109 Size: 1189 367 4412 Fragments arranged by size: 4412 1189 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2323 2476 2840 3289 4744 Size: 325 217 627 536 153 364 449 1455 Cuts at: 4744 618 Size: 1842 Fragments arranged by size: 1842 1455 627 536 449 364 325 217 153 BanII G_rGCy'C Cuts at: 1391 2920 2932 3287 1391 Size: 1529 12 355 4072 Fragments arranged by size: 4072 1529 355 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4997 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4159 4193 1903 Size: 34 2222 34 3678 Fragments arranged by size: 3678 2222 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3860 5070 3860 Size: 1210 4758 BclI T'GATC_A Cuts at: 1548 1548 Size: 5968 BfrBI ATG'CAT Cuts at: 2708 2708 Size: 5968 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4579 136 Size: 107 122 71 631 605 2907 1525 Fragments arranged by size: 2907 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5963 5963 Size: 5968 BlpI GC'TnA_GC Cuts at: 2645 2814 3385 2645 Size: 169 571 5228 Fragments arranged by size: 5228 571 169 Bme1580I G_kGCm'C Cuts at: 1790 2328 2920 4029 5185 1790 Size: 538 592 1109 1156 2573 Fragments arranged by size: 2573 1156 1109 592 538 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5968 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2219 3699 4657 Size: 416 53 83 399 24 802 1480 958 Cuts at: 4657 442 Size: 1753 Fragments arranged by size: 1753 1480 958 802 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5968 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4628 1239 Size: 3389 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2906 4093 4958 5256 2906 Size: 1187 865 298 3618 Fragments arranged by size: 3618 1187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4631 882 Size: 617 3132 2219 Fragments arranged by size: 3132 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3759 493 Size: 711 2555 2702 Fragments arranged by size: 2702 2555 711 BsaBI GATnn'nnATC Cuts at: 5962 5962 Size: 5968 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3775 4157 4792 275 Size: 53 83 186 936 2242 382 635 1451 Fragments arranged by size: 2242 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3012 4401 4799 5073 5220 5631 3012 Size: 1389 398 274 147 411 3349 Fragments arranged by size: 3349 1389 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2948 2978 3140 3170 1178 Size: 30 165 30 1545 30 162 30 3976 Fragments arranged by size: 3976 1545 165 162 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2392 2897 3148 3151 1186 Size: 1206 505 251 3 4003 Fragments arranged by size: 4003 1206 505 251 3 BseYI C'CCAG_C Cuts at: 2639 5171 5667 2639 Size: 2532 496 2940 Fragments arranged by size: 2940 2532 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2297 2849 1163 Size: 573 561 552 4282 Fragments arranged by size: 4282 573 561 552 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3418 4179 5207 1540 Size: 275 186 1417 761 1028 2301 Fragments arranged by size: 2301 1417 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2932 4029 4114 5185 2932 Size: 1097 85 1071 3715 Fragments arranged by size: 3715 1097 1071 85 BsmI GAATG_Cn' Cuts at: 3479 3572 3479 Size: 93 5875 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 2920 2932 3287 4029 4114 5185 Size: 399 538 592 12 355 742 85 1071 Cuts at: 5185 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 592 538 399 355 85 12 BspEI T'CCGG_A Cuts at: 5631 5631 Size: 5968 BspHI T'CATG_A Cuts at: 3857 5587 3857 Size: 1730 4238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3323 844 Size: 2479 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3855 1394 Size: 429 2032 3507 Fragments arranged by size: 3507 2032 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4463 4637 65 Size: 1183 3215 174 1396 Fragments arranged by size: 3215 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3012 4612 4799 1700 Size: 110 1202 1600 187 2869 Fragments arranged by size: 2869 1600 1202 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5968 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5968 BssSI C'ACGA_G Cuts at: 4028 5040 4028 Size: 1012 4956 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5968 BstBI TT'CG_AA Cuts at: 2260 2740 4772 2260 Size: 480 2032 3456 Fragments arranged by size: 3456 2032 480 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5968 BstXI CCAn_nnnn'nTGG Cuts at: 2639 2639 Size: 5968 BstYI r'GATC_y Cuts at: 1972 2109 3298 3665 4050 4067 5519 5605 5617 Size: 137 1189 367 385 17 1452 86 12 Cuts at: 5617 5963 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1189 385 367 346 137 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2614 3220 2614 Size: 606 5362 BtgI C'CryG_G Cuts at: 513 1065 2004 2319 2472 513 Size: 552 939 315 153 4009 Fragments arranged by size: 4009 939 552 315 153 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5229 Fragments arranged by size: 5229 683 56 BtsI GCAGTG_nn' Cuts at: 3484 4278 4298 3484 Size: 794 20 5154 Fragments arranged by size: 5154 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5968 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5933 DraI TTT'AAA Cuts at: 3272 3272 Size: 5968 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2761 4975 809 Size: 507 1445 2214 1802 Fragments arranged by size: 2214 1802 1445 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3415 4304 5933 Size: 54 1425 50 275 18 1585 889 1629 Cuts at: 5933 8 Size: 43 Fragments arranged by size: 1629 1585 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3415 1537 Size: 275 1603 4090 Fragments arranged by size: 4090 1603 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2369 3215 3898 1353 Size: 1016 846 683 3423 Fragments arranged by size: 3423 1016 846 683 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4555 4929 5075 387 Size: 1064 138 435 2531 374 146 1280 Fragments arranged by size: 2531 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2930 2930 Size: 5968 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 3221 4031 4628 5415 5683 1239 Size: 393 821 768 810 597 787 268 1524 Fragments arranged by size: 1524 821 810 787 768 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2674 2916 2917 3225 2674 Size: 242 1 308 5417 Fragments arranged by size: 5417 308 242 1 EcoRI G'AATT_C Cuts at: 3251 3277 3655 3251 Size: 26 378 5564 Fragments arranged by size: 5564 378 26 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2081 2386 3226 2081 Size: 305 840 4823 Fragments arranged by size: 4823 840 305 FspI TGC'GCA Cuts at: 4474 4474 Size: 5968 HaeII r_GCGC'y Cuts at: 1678 5115 1678 Size: 3437 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2094 2126 2247 2279 2413 Size: 32 135 32 522 32 121 32 134 Cuts at: 2413 2445 3095 3127 4609 4641 4683 4715 1373 Size: 32 650 32 1482 32 42 32 2626 Fragments arranged by size: 2626 1482 650 522 135 134 121 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2136 3312 3558 669 Size: 988 281 198 1176 246 3079 Fragments arranged by size: 3079 1176 988 281 246 198 HindIII A'AGCT_T Cuts at: 748 2234 2657 3263 748 Size: 1486 423 606 3453 Fragments arranged by size: 3453 1486 606 423 HpaI GTT'AAC Cuts at: 3558 3558 Size: 5968 KpnI G_GTAC'C Cuts at: 3293 3293 Size: 5968 MfeI C'AATT_G Cuts at: 3567 3567 Size: 5968 MluI A'CGCG_T Cuts at: 5844 5844 Size: 5968 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2065 3189 5082 5266 1305 Size: 760 1124 1893 184 2007 Fragments arranged by size: 2007 1893 1124 760 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4146 Fragments arranged by size: 4146 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2690 2846 3054 3926 4285 4444 518 Size: 648 1524 156 208 872 359 159 2042 Fragments arranged by size: 2042 1524 872 648 359 208 159 156 MspA1I CmG'CkG Cuts at: 1606 2810 3384 4061 5209 5454 1606 Size: 1204 574 677 1148 245 2120 Fragments arranged by size: 2120 1204 1148 677 574 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2472 513 Size: 552 939 468 4009 Fragments arranged by size: 4009 939 552 468 NdeI CA'TA_TG Cuts at: 387 387 Size: 5968 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5968 NotI GC'GGCC_GC Cuts at: 3415 3415 Size: 5968 NsiI A_TGCA'T Cuts at: 2710 2710 Size: 5968 NspI r_CATG'y Cuts at: 1132 1229 2764 3326 4871 1132 Size: 97 1535 562 1545 2229 Fragments arranged by size: 2229 1545 1535 562 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5968 PciI A'CATG_T Cuts at: 2760 4867 2760 Size: 2107 3861 PflMI CCAn_nnn'nTGG Cuts at: 1108 2411 2635 1108 Size: 1303 224 4441 Fragments arranged by size: 4441 1303 224 PfoI T'CCnGG_A Cuts at: 2765 2765 Size: 5968 PmeI GTTT'AAAC Cuts at: 3272 3272 Size: 5968 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3140 3172 4037 4069 5550 5582 3140 Size: 32 865 32 1481 32 3526 Fragments arranged by size: 3526 1481 865 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5968 PsiI TTA'TAA Cuts at: 3538 3538 Size: 5968 PspOMI G'GGCC_C Cuts at: 2916 2916 Size: 5968 PspXI vC'TCGA_Gb Cuts at: 2871 2871 Size: 5968 PstI C_TGCA'G Cuts at: 830 3320 830 Size: 2490 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5968 PvuII CAG'CTG Cuts at: 2810 2810 Size: 5968 SacI G_AGCT'C Cuts at: 2932 2932 Size: 5968 SalI G'TCGA_C Cuts at: 1936 3310 1936 Size: 1374 4594 SbfI CC_TGCA'GG Cuts at: 3320 3320 Size: 5968 ScaI AGT'ACT Cuts at: 4216 5928 4216 Size: 1712 4256 SfcI C'TryA_G Cuts at: 826 1045 3316 4451 5132 5323 826 Size: 219 2271 1135 681 191 1471 Fragments arranged by size: 2271 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5968 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5968 SmaI CCC'GGG Cuts at: 1872 2228 2357 3295 1872 Size: 356 129 938 4545 Fragments arranged by size: 4545 938 356 129 SmlI C'TyrA_G Cuts at: 820 1017 1943 2100 2871 2921 4072 4973 5235 Size: 197 926 157 771 50 1151 901 262 Cuts at: 5235 820 Size: 1553 Fragments arranged by size: 1553 1151 926 901 771 262 197 157 50 SnaBI TAC'GTA Cuts at: 493 3759 493 Size: 3266 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5968 SphI G_CATG'C Cuts at: 1132 3326 1132 Size: 2194 3774 SspI AAT'ATT Cuts at: 5 52 3892 5 Size: 47 3840 2081 Fragments arranged by size: 3840 2081 47 StuI AGG'CCT Cuts at: 1517 2121 1517 Size: 604 5364 StyI C'CwwG_G Cuts at: 513 1065 2004 2472 2670 3407 513 Size: 552 939 468 198 737 3074 Fragments arranged by size: 3074 939 737 552 468 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2422 3995 4012 4165 4350 4795 325 Size: 1255 842 1573 17 153 185 445 1498 Fragments arranged by size: 1573 1498 1255 842 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3003 4214 5926 Size: 274 80 33 51 1443 1026 1211 1712 Cuts at: 5926 96 Size: 138 Fragments arranged by size: 1712 1443 1211 1026 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5928 5960 5928 Size: 32 5936 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5968 XbaI T'CTAG_A Cuts at: 3304 3304 Size: 5968 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2636 2636 Size: 5968 XhoI C'TCGA_G Cuts at: 1943 2100 2871 1943 Size: 157 771 5040 Fragments arranged by size: 5040 771 157 XmaI C'CCGG_G Cuts at: 1870 2226 2355 3293 1870 Size: 356 129 938 4545 Fragments arranged by size: 4545 938 356 129 XmnI GAAnn'nnTTC Cuts at: 4097 4097 Size: 5968 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3776 276 Size: 53 83 186 936 2242 2468 Fragments arranged by size: 2468 2242 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI