Restriction map for
pFN21AA1649
(Linear) (Six-base) MAPSORT of: KIAA1649.seq Check: 958 from: 1 to: 1263
KIAA1649.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1216 1263
Size: 448 768 47
Fragments arranged by size:
768 448 47
AflIII A'CryG_T
Cuts at: 0 755 1263
Size: 755 508
AgeI A'CCGG_T
Cuts at: 0 1007 1263
Size: 1007 256
AleI CACnn'nnGTG
Cuts at: 0 685 1263
Size: 685 578
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 260 1263
Size: 260 1003
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1135 1167 1263
Size: 1135 32 96
Fragments arranged by size:
1135 96 32
AlwNI CAG_nnn'CTG
Cuts at: 0 715 808 1263
Size: 715 93 455
Fragments arranged by size:
715 455 93
ApaI G_GGCC'C
Cuts at: 0 915 1263
Size: 915 348
ApoI r'AATT_y
Cuts at: 0 537 1246 1263
Size: 537 709 17
Fragments arranged by size:
709 537 17
AvaI C'yCGr_G
Cuts at: 0 95 221 350 866 1263
Size: 95 126 129 516 397
Fragments arranged by size:
516 397 129 126 95
BamHI G'GATC_C
Cuts at: 0 104 1263
Size: 104 1159
BanI G'GyrC_C
Cuts at: 0 318 471 835 1263
Size: 318 153 364 428
Fragments arranged by size:
428 364 318 153
BanII G_rGCy'C
Cuts at: 0 915 927 1263
Size: 915 12 336
Fragments arranged by size:
915 336 12
BfrBI ATG'CAT
Cuts at: 0 703 1263
Size: 703 560
BlpI GC'TnA_GC
Cuts at: 0 640 809 1263
Size: 640 169 454
Fragments arranged by size:
640 454 169
Bme1580I G_kGCm'C
Cuts at: 0 323 915 1263
Size: 323 592 348
Fragments arranged by size:
592 348 323
BmrI ACTGGGnnnn_n'
Cuts at: 0 214 1263
Size: 214 1049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1263
Size: 901 362
BsaWI w'CCGG_w
Cuts at: 0 1007 1263
Size: 1007 256
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 943 973 1135 1165 1263
Size: 943 30 162 30 98
Fragments arranged by size:
943 162 98 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 387 892 1143 1146 1263
Size: 387 505 251 3 117
Fragments arranged by size:
505 387 251 117 3
BseYI C'CCAG_C
Cuts at: 0 634 1263
Size: 634 629
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 844 1263
Size: 292 552 419
Fragments arranged by size:
552 419 292
BsiHKAI G_wGCw'C
Cuts at: 0 927 1263
Size: 927 336
Bsp1286I G_dGCh'C
Cuts at: 0 323 915 927 1263
Size: 323 592 12 336
Fragments arranged by size:
592 336 323 12
BsrFI r'CCGG_y
Cuts at: 0 1007 1263
Size: 1007 256
BstBI TT'CG_AA
Cuts at: 0 255 735 1263
Size: 255 480 528
Fragments arranged by size:
528 480 255
BstXI CCAn_nnnn'nTGG
Cuts at: 0 634 1263
Size: 634 629
BstYI r'GATC_y
Cuts at: 0 104 1263
Size: 104 1159
Bsu36I CC'TnA_GG
Cuts at: 0 609 1215 1263
Size: 609 606 48
Fragments arranged by size:
609 606 48
BtgI C'CryG_G
Cuts at: 0 314 467 1263
Size: 314 153 796
Fragments arranged by size:
796 314 153
DrdI GACnn_nn'nnGTC
Cuts at: 0 756 1263
Size: 756 507
EarI CTCTTCn'nnn_
Cuts at: 0 364 1210 1263
Size: 364 846 53
Fragments arranged by size:
846 364 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1263
Size: 19 1244
EcoICRI GAG'CTC
Cuts at: 0 925 1263
Size: 925 338
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1216 1263
Size: 448 768 47
Fragments arranged by size:
768 448 47
EcoO109I rG'GnC_Cy
Cuts at: 0 669 911 912 1220 1263
Size: 669 242 1 308 43
Fragments arranged by size:
669 308 242 43 1
EcoRI G'AATT_C
Cuts at: 0 1246 1263
Size: 1246 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 76 381 1221 1263
Size: 76 305 840 42
Fragments arranged by size:
840 305 76 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 89 121 242 274 408 440 1090 1122
Size: 89 32 121 32 134 32 650 32
Cuts at: 1122 1263
Size: 141
Fragments arranged by size:
650 141 134 121 89 32 32 32
32
HincII GTy'rAC
Cuts at: 0 131 1263
Size: 131 1132
HindIII A'AGCT_T
Cuts at: 0 229 652 1258 1263
Size: 229 423 606 5
Fragments arranged by size:
606 423 229 5
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 60 1184 1263
Size: 60 1124 79
Fragments arranged by size:
1124 79 60
MslI CAynn'nnrTG
Cuts at: 0 685 841 1049 1263
Size: 685 156 208 214
Fragments arranged by size:
685 214 208 156
MspA1I CmG'CkG
Cuts at: 0 805 1263
Size: 805 458
NcoI C'CATG_G
Cuts at: 0 467 1263
Size: 467 796
NsiI A_TGCA'T
Cuts at: 0 705 1263
Size: 705 558
NspI r_CATG'y
Cuts at: 0 759 1263
Size: 759 504
PciI A'CATG_T
Cuts at: 0 755 1263
Size: 755 508
PflMI CCAn_nnn'nTGG
Cuts at: 0 406 630 1263
Size: 406 224 633
Fragments arranged by size:
633 406 224
PfoI T'CCnGG_A
Cuts at: 0 760 1263
Size: 760 503
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1135 1167 1263
Size: 1135 32 96
Fragments arranged by size:
1135 96 32
PspOMI G'GGCC_C
Cuts at: 0 911 1263
Size: 911 352
PspXI vC'TCGA_Gb
Cuts at: 0 866 1263
Size: 866 397
PvuII CAG'CTG
Cuts at: 0 805 1263
Size: 805 458
SacI G_AGCT'C
Cuts at: 0 927 1263
Size: 927 336
SmaI CCC'GGG
Cuts at: 0 223 352 1263
Size: 223 129 911
Fragments arranged by size:
911 223 129
SmlI C'TyrA_G
Cuts at: 0 95 866 916 1263
Size: 95 771 50 347
Fragments arranged by size:
771 347 95 50
StuI AGG'CCT
Cuts at: 0 116 1263
Size: 116 1147
StyI C'CwwG_G
Cuts at: 0 467 665 1263
Size: 467 198 598
Fragments arranged by size:
598 467 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 417 1263
Size: 417 846
TatI w'GTAC_w
Cuts at: 0 998 1263
Size: 998 265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 631 1263
Size: 631 632
XhoI C'TCGA_G
Cuts at: 0 95 866 1263
Size: 95 771 397
Fragments arranged by size:
771 397 95
XmaI C'CCGG_G
Cuts at: 0 221 350 1263
Size: 221 129 913
Fragments arranged by size:
913 221 129
Enzymes that do cut:
AcuI AflIII AgeI AleI AlfI AloI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BfrBI BlpI Bme1580I
BmrI BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BsrFI BstBI BstXI BstYI Bsu36I BtgI DrdI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I
HincII HindIII MmeI MslI MspA1I NcoI NsiI NspI
PciI PflMI PfoI PpiI PspOMI PspXI PvuII SacI
SmaI SmlI StuI StyI TaqII TatI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BmgBI BmtI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstEII BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII EaeI EagI EcoNI EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PpuMI PshAI PsiI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XmnI ZraI