(Circular) (Six-base) MAPSORT of: pFN21AA1645.seq Check: 4925 from: 1 to: 5686 pFN21AA1645 5686 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2725 2725 Size: 5686 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2453 2589 3496 278 Size: 53 83 186 936 917 136 907 2468 Fragments arranged by size: 2468 936 917 907 186 136 83 53 AccI GT'mk_AC Cuts at: 1937 3029 1937 Size: 1092 4594 Acc65I G'GTAC_C Cuts at: 3007 3007 Size: 5686 AclI AA'CG_TT Cuts at: 1563 3813 4186 1563 Size: 2250 373 3063 Fragments arranged by size: 3063 2250 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2294 2486 3749 5133 5401 1632 Size: 662 192 1263 1384 268 1917 Fragments arranged by size: 1917 1384 1263 662 268 192 AfeI AGC'GCT Cuts at: 2311 2311 Size: 5686 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5489 AflIII A'CryG_T Cuts at: 1203 4585 5562 1203 Size: 3382 977 1327 Fragments arranged by size: 3382 1327 977 AgeI A'CCGG_T Cuts at: 2068 4517 2068 Size: 2449 3237 AhdI GACnn_n'nnGTC Cuts at: 4415 4415 Size: 5686 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5686 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5686 AlwNI CAG_nnn'CTG Cuts at: 2233 2431 2731 2866 2964 3429 5001 2233 Size: 198 300 135 98 465 1572 2918 Fragments arranged by size: 2918 1572 465 300 198 135 98 ApaLI G'TGCA_C Cuts at: 3743 4899 3743 Size: 1156 4530 ApoI r'AATT_y Cuts at: 1493 2995 3221 3373 5482 1493 Size: 1502 226 152 2109 1697 Fragments arranged by size: 2109 1697 1502 226 152 AseI AT'TA_AT Cuts at: 160 1032 4240 160 Size: 872 3208 1606 Fragments arranged by size: 3208 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 AvaI C'yCGr_G Cuts at: 1870 1943 2765 3011 1870 Size: 73 822 246 4545 Fragments arranged by size: 4545 822 246 73 BamHI G'GATC_C Cuts at: 3016 3383 3016 Size: 367 5319 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2057 2112 2244 3007 4462 Size: 325 217 627 270 55 132 763 1455 Cuts at: 4462 618 Size: 1842 Fragments arranged by size: 1842 1455 763 627 325 270 217 132 55 BanII G_rGCy'C Cuts at: 1391 2101 2634 2942 3005 1391 Size: 710 533 308 63 4072 Fragments arranged by size: 4072 710 533 308 63 BbeI G_GCGC'C Cuts at: 2116 2248 2116 Size: 132 5554 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4715 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2596 2630 3877 3911 1903 Size: 34 659 34 1247 34 3678 Fragments arranged by size: 3678 1247 659 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3578 4788 3578 Size: 1210 4476 BclI T'GATC_A Cuts at: 1548 1548 Size: 5686 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2205 2242 Size: 107 122 71 631 605 337 196 37 Cuts at: 2242 2876 4297 136 Size: 634 1421 1525 Fragments arranged by size: 1525 1421 634 631 605 337 196 122 107 71 37 BglII A'GATC_T Cuts at: 5681 5681 Size: 5686 BlpI GC'TnA_GC Cuts at: 3103 3103 Size: 5686 Bme1580I G_kGCm'C Cuts at: 1790 3747 4903 1790 Size: 1957 1156 2573 Fragments arranged by size: 2573 1957 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5686 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2035 2056 3417 Size: 416 53 83 399 24 618 21 1361 Cuts at: 3417 4375 442 Size: 958 1753 Fragments arranged by size: 1753 1361 958 618 416 399 83 53 24 21 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5686 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2458 2458 Size: 5686 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2016 2179 2453 2728 4346 1239 Size: 777 163 274 275 1618 2579 Fragments arranged by size: 2579 1618 777 275 274 163 Bpu10I CC'TnA_GC Cuts at: 2106 2106 Size: 5686 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3811 4676 4974 3811 Size: 865 298 4523 Fragments arranged by size: 4523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2139 4349 882 Size: 617 640 2210 2219 Fragments arranged by size: 2219 2210 640 617 BsaAI yAC'GTr Cuts at: 493 1204 3477 493 Size: 711 2273 2702 Fragments arranged by size: 2702 2273 711 BsaBI GATnn'nnATC Cuts at: 5680 5680 Size: 5686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2113 2245 2450 2586 Size: 53 83 186 936 580 132 205 136 Cuts at: 2586 3493 3875 4510 275 Size: 907 382 635 1451 Fragments arranged by size: 1451 936 907 635 580 382 205 186 136 132 83 53 BsaWI w'CCGG_w Cuts at: 2068 4119 4517 4791 4938 5349 2068 Size: 2051 398 274 147 411 2405 Fragments arranged by size: 2405 2051 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2516 2546 2639 2669 1178 Size: 30 165 30 1113 30 93 30 4195 Fragments arranged by size: 4195 1113 165 93 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2243 2642 1186 Size: 925 132 399 4230 Fragments arranged by size: 4230 925 399 132 BseYI C'CCAG_C Cuts at: 2488 2820 4889 5385 2488 Size: 332 2069 496 2789 Fragments arranged by size: 2789 2069 496 332 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2066 2626 1163 Size: 573 330 560 4223 Fragments arranged by size: 4223 573 560 330 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3136 3897 4925 1540 Size: 275 186 1135 761 1028 2301 Fragments arranged by size: 2301 1135 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3747 3832 4903 3747 Size: 85 1071 4530 Fragments arranged by size: 4530 1071 85 BsmI GAATG_Cn' Cuts at: 3197 3290 3197 Size: 93 5593 BsmBI CGTCTCn'nnnn_ Cuts at: 2593 2593 Size: 5686 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2101 2634 2942 3005 3747 3832 4903 Size: 399 311 533 308 63 742 85 1071 Cuts at: 4903 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 533 399 311 308 85 63 BspEI T'CCGG_A Cuts at: 5349 5349 Size: 5686 BspHI T'CATG_A Cuts at: 2749 3575 5305 2749 Size: 826 1730 3130 Fragments arranged by size: 3130 1730 826 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2725 3041 844 Size: 1881 316 3489 Fragments arranged by size: 3489 1881 316 BsrBI CCG'CTC Cuts at: 1394 1823 2119 2180 3573 1394 Size: 429 296 61 1393 3507 Fragments arranged by size: 3507 1393 429 296 61 BsrDI GCAATG_nn' Cuts at: 65 1248 4181 4355 65 Size: 1183 2933 174 1396 Fragments arranged by size: 2933 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2068 2552 4330 4517 1700 Size: 110 258 484 1778 187 2869 Fragments arranged by size: 2869 1778 484 258 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5686 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5686 BssSI C'ACGA_G Cuts at: 3746 4758 3746 Size: 1012 4674 BstAPI GCAn_nnn'nTGC Cuts at: 794 2080 2233 2619 2731 794 Size: 1286 153 386 112 3749 Fragments arranged by size: 3749 1286 386 153 112 BstBI TT'CG_AA Cuts at: 4490 4490 Size: 5686 BstEII G'GTnAC_C Cuts at: 1184 2182 1184 Size: 998 4688 BstYI r'GATC_y Cuts at: 1972 2802 3016 3383 3768 3785 5237 5323 5335 Size: 830 214 367 385 17 1452 86 12 Cuts at: 5335 5681 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 830 385 367 346 214 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2200 2685 2934 513 Size: 552 1135 485 249 3265 Fragments arranged by size: 3265 1135 552 485 249 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2599 487 Size: 683 56 1373 3574 Fragments arranged by size: 3574 1373 683 56 BtsI GCAGTG_nn' Cuts at: 3202 3996 4016 3202 Size: 794 20 4872 Fragments arranged by size: 4872 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5686 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5651 DraI TTT'AAA Cuts at: 2990 2990 Size: 5686 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4693 809 Size: 507 3377 1802 Fragments arranged by size: 3377 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2197 2206 2367 Size: 54 1425 50 275 18 367 9 161 Cuts at: 2367 2550 2789 2808 2868 2931 3133 4022 5651 Size: 183 239 19 60 63 202 889 1629 Cuts at: 5651 8 Size: 43 Fragments arranged by size: 1629 1425 889 367 275 239 202 183 161 63 60 54 50 43 19 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3133 1537 Size: 275 1321 4090 Fragments arranged by size: 4090 1321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2129 3616 1353 Size: 776 1487 3423 Fragments arranged by size: 3423 1487 776 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2866 4273 4647 4793 387 Size: 1064 138 1277 1407 374 146 1280 Fragments arranged by size: 1407 1280 1277 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2016 2179 2294 2453 2486 2728 3749 Size: 393 384 163 115 159 33 242 1021 Cuts at: 3749 4346 5133 5401 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1021 787 597 393 384 268 242 163 159 115 33 EcoNI CCTnn'n_nnAGG Cuts at: 2258 2297 2258 Size: 39 5647 EcoO109I rG'GnC_Cy Cuts at: 2269 2290 2728 2269 Size: 21 438 5227 Fragments arranged by size: 5227 438 21 EcoRI G'AATT_C Cuts at: 2995 3373 2995 Size: 378 5308 FspI TGC'GCA Cuts at: 4192 4192 Size: 5686 HaeII r_GCGC'y Cuts at: 1678 2116 2248 2313 2699 2892 4833 1678 Size: 438 132 65 386 193 1941 2531 Fragments arranged by size: 2531 1941 438 386 193 132 65 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2516 2548 2552 2559 2584 Size: 32 135 32 944 32 4 7 25 Cuts at: 2584 2591 2639 2671 4327 4359 4401 4433 1373 Size: 7 48 32 1656 32 42 32 2626 Fragments arranged by size: 2626 1656 944 135 48 42 32 32 32 32 32 32 25 7 7 4 HincII GTy'rAC Cuts at: 669 1657 1938 3030 3276 669 Size: 988 281 1092 246 3079 Fragments arranged by size: 3079 1092 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5686 HpaI GTT'AAC Cuts at: 3276 3276 Size: 5686 KasI G'GCGC_C Cuts at: 2112 2244 2112 Size: 132 5554 KpnI G_GTAC'C Cuts at: 3011 3011 Size: 5686 MfeI C'AATT_G Cuts at: 3285 3285 Size: 5686 MluI A'CGCG_T Cuts at: 5562 5562 Size: 5686 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4800 4984 1305 Size: 3495 184 2007 Fragments arranged by size: 3495 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2199 2810 10 Size: 54 1425 343 367 611 2886 Fragments arranged by size: 2886 1425 611 367 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2498 2837 3644 4003 4162 518 Size: 648 1332 339 807 359 159 2042 Fragments arranged by size: 2042 1332 807 648 359 339 159 MspA1I CmG'CkG Cuts at: 1606 2687 2894 2936 2953 3102 3779 4927 5172 Size: 1081 207 42 17 149 677 1148 245 Cuts at: 5172 1606 Size: 2120 Fragments arranged by size: 2120 1148 1081 677 245 207 149 42 17 NaeI GCC'GGC Cuts at: 2554 2554 Size: 5686 NarI GG'CG_CC Cuts at: 2113 2245 2113 Size: 132 5554 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5134 NdeI CA'TA_TG Cuts at: 387 387 Size: 5686 NgoMIV G'CCGG_C Cuts at: 2552 2552 Size: 5686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5686 NotI GC'GGCC_GC Cuts at: 3133 3133 Size: 5686 NspI r_CATG'y Cuts at: 1132 1229 2078 2497 3044 4589 1132 Size: 97 849 419 547 1545 2229 Fragments arranged by size: 2229 1545 849 547 419 97 PasI CC'CwG_GG Cuts at: 1348 2239 1348 Size: 891 4795 PciI A'CATG_T Cuts at: 4585 4585 Size: 5686 PflMI CCAn_nnn'nTGG Cuts at: 1108 2905 1108 Size: 1797 3889 PmeI GTTT'AAAC Cuts at: 2990 2990 Size: 5686 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3755 3787 5268 5300 3755 Size: 32 1481 32 4141 Fragments arranged by size: 4141 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2269 2290 2269 Size: 21 5665 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5686 PsiI TTA'TAA Cuts at: 3256 3256 Size: 5686 PstI C_TGCA'G Cuts at: 830 2169 2229 2531 2736 3038 830 Size: 1339 60 302 205 302 3478 Fragments arranged by size: 3478 1339 302 302 205 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5686 SacII CC_GC'GG Cuts at: 2688 2937 2688 Size: 249 5437 SalI G'TCGA_C Cuts at: 1936 3028 1936 Size: 1092 4594 SbfI CC_TGCA'GG Cuts at: 2736 3038 2736 Size: 302 5384 ScaI AGT'ACT Cuts at: 3934 5646 3934 Size: 1712 3974 SfcI C'TryA_G Cuts at: 826 1045 2165 2225 2527 2732 3034 4169 4850 Size: 219 1120 60 302 205 302 1135 681 Cuts at: 4850 5041 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1120 681 302 302 219 205 191 60 SfiI GGCCn_nnn'nGGCC Cuts at: 2205 2876 2205 Size: 671 5015 SfoI GGC'GCC Cuts at: 2114 2246 2114 Size: 132 5554 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5686 SgrAI Cr'CCGG_yG Cuts at: 1700 2068 1700 Size: 368 5318 SmaI CCC'GGG Cuts at: 1872 2767 3013 1872 Size: 895 246 4545 Fragments arranged by size: 4545 895 246 SmlI C'TyrA_G Cuts at: 820 1017 1943 3790 4691 4953 820 Size: 197 926 1847 901 262 1553 Fragments arranged by size: 1847 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3477 493 Size: 2984 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5686 SphI G_CATG'C Cuts at: 1132 2078 2497 3044 1132 Size: 946 419 547 3774 Fragments arranged by size: 3774 946 547 419 SspI AAT'ATT Cuts at: 5 52 3610 5 Size: 47 3558 2081 Fragments arranged by size: 3558 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5686 StyI C'CwwG_G Cuts at: 513 1065 2943 3125 513 Size: 552 1878 182 3074 Fragments arranged by size: 3074 1878 552 182 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2075 3713 3730 3883 4068 4513 325 Size: 1255 495 1638 17 153 185 445 1498 Fragments arranged by size: 1638 1498 1255 495 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3932 5644 96 Size: 274 80 33 51 1443 1955 1712 138 Fragments arranged by size: 1955 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2312 2344 5646 5678 2312 Size: 32 3302 32 2320 Fragments arranged by size: 3302 2320 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5686 XbaI T'CTAG_A Cuts at: 3022 3022 Size: 5686 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5686 XmaI C'CCGG_G Cuts at: 1870 2765 3011 1870 Size: 895 246 4545 Fragments arranged by size: 4545 895 246 XmnI GAAnn'nnTTC Cuts at: 3815 3815 Size: 5686 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2451 2587 3494 276 Size: 53 83 186 936 917 136 907 2468 Fragments arranged by size: 2468 936 917 907 186 136 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SrfI SwaI XcmI