(Circular) (Six-base) MAPSORT of: pFN21AA1622.seq Check: 8384 from: 1 to: 7324 pFN21AA1622 7324 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5134 278 Size: 53 83 186 936 3598 2468 Fragments arranged by size: 3598 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3398 4667 1937 Size: 1461 1269 4594 Fragments arranged by size: 4594 1461 1269 Acc65I G'GTAC_C Cuts at: 4645 4645 Size: 7324 AclI AA'CG_TT Cuts at: 1563 5451 5824 1563 Size: 3888 373 3063 Fragments arranged by size: 3888 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3249 3509 3923 3932 5387 6771 7039 1632 Size: 1617 260 414 9 1455 1384 268 1917 Fragments arranged by size: 1917 1617 1455 1384 414 268 260 9 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7127 AflIII A'CryG_T Cuts at: 1203 3547 6223 7200 1203 Size: 2344 2676 977 1327 Fragments arranged by size: 2676 2344 1327 977 AgeI A'CCGG_T Cuts at: 6155 6155 Size: 7324 AhdI GACnn_n'nnGTC Cuts at: 3698 6053 3698 Size: 2355 4969 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7324 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3680 1184 Size: 2496 4828 AlwNI CAG_nnn'CTG Cuts at: 3473 5067 6639 3473 Size: 1594 1572 4158 Fragments arranged by size: 4158 1594 1572 ApaLI G'TGCA_C Cuts at: 5381 6537 5381 Size: 1156 6168 ApoI r'AATT_y Cuts at: 1493 2589 2612 2982 3017 3286 3776 4633 4859 Size: 1096 23 370 35 269 490 857 226 Cuts at: 4859 5011 7120 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1096 857 490 370 269 226 152 35 23 AseI AT'TA_AT Cuts at: 160 1032 2771 3713 5878 160 Size: 872 1739 942 2165 1606 Fragments arranged by size: 2165 1739 1606 942 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7324 AvaI C'yCGr_G Cuts at: 1870 1943 4649 1870 Size: 73 2706 4545 Fragments arranged by size: 4545 2706 73 BamHI G'GATC_C Cuts at: 4654 5021 4654 Size: 367 6957 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4645 6100 618 Size: 325 217 627 2858 1455 1842 Fragments arranged by size: 2858 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2085 4643 1391 Size: 694 2558 4072 Fragments arranged by size: 4072 2558 694 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2163 928 Size: 971 264 6089 Fragments arranged by size: 6089 971 264 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3636 3670 5515 5549 1903 Size: 34 50 34 1615 34 1845 34 3678 Fragments arranged by size: 3678 1845 1615 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3760 5216 6426 3760 Size: 1456 1210 4658 Fragments arranged by size: 4658 1456 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7324 BfrBI ATG'CAT Cuts at: 2008 2390 2459 3657 2008 Size: 382 69 1198 5675 Fragments arranged by size: 5675 1198 382 69 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5935 136 Size: 107 122 71 631 605 4263 1525 Fragments arranged by size: 4263 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7319 7319 Size: 7324 BlpI GC'TnA_GC Cuts at: 4741 4741 Size: 7324 Bme1580I G_kGCm'C Cuts at: 1790 5385 6541 1790 Size: 3595 1156 2573 Fragments arranged by size: 3595 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7324 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5055 6013 442 Size: 416 53 83 399 24 3638 958 1753 Fragments arranged by size: 3638 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7324 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2450 2976 3312 3365 5984 1239 Size: 1211 526 336 53 2619 2579 Fragments arranged by size: 2619 2579 1211 526 336 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2102 3025 5449 6314 6612 2102 Size: 923 2424 865 298 2814 Fragments arranged by size: 2814 2424 923 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5987 882 Size: 617 4488 2219 Fragments arranged by size: 4488 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5115 493 Size: 711 3911 2702 Fragments arranged by size: 3911 2702 711 BsaBI GATnn'nnATC Cuts at: 3930 7318 3930 Size: 3388 3936 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5131 5513 6148 275 Size: 53 83 186 936 3598 382 635 1451 Fragments arranged by size: 3598 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2125 4464 5757 6155 6429 6576 6987 2125 Size: 2339 1293 398 274 147 411 2462 Fragments arranged by size: 2462 2339 1293 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7099 Fragments arranged by size: 7099 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7324 BseYI C'CCAG_C Cuts at: 6527 7023 6527 Size: 496 6828 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6751 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4774 5535 6563 1540 Size: 275 186 2773 761 1028 2301 Fragments arranged by size: 2773 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2085 2436 2763 5385 5470 6541 2085 Size: 351 327 2622 85 1071 2868 Fragments arranged by size: 2868 2622 1071 351 327 85 BsmI GAATG_Cn' Cuts at: 4835 4928 4835 Size: 93 7231 BsmBI CGTCTCn'nnnn_ Cuts at: 2261 2261 Size: 7324 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2436 2763 4643 5385 5470 6541 Size: 399 295 351 327 1880 742 85 1071 Cuts at: 6541 1391 Size: 2174 Fragments arranged by size: 2174 1880 1071 742 399 351 327 295 85 BspEI T'CCGG_A Cuts at: 4464 6987 4464 Size: 2523 4801 BspHI T'CATG_A Cuts at: 3511 3603 5213 6943 3511 Size: 92 1610 1730 3892 Fragments arranged by size: 3892 1730 1610 92 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2082 3838 4679 844 Size: 1238 1756 841 3489 Fragments arranged by size: 3489 1756 1238 841 BsrBI CCG'CTC Cuts at: 1394 1823 5211 1394 Size: 429 3388 3507 Fragments arranged by size: 3507 3388 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2461 5819 5993 65 Size: 1183 1213 3358 174 1396 Fragments arranged by size: 3358 1396 1213 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2101 5968 6155 1700 Size: 110 291 3867 187 2869 Fragments arranged by size: 3867 2869 291 187 110 BsrGI T'GTAC_A Cuts at: 96 3035 96 Size: 2939 4385 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7324 BssSI C'ACGA_G Cuts at: 5384 6396 5384 Size: 1012 6312 BstAPI GCAn_nnn'nTGC Cuts at: 794 4231 794 Size: 3437 3887 BstBI TT'CG_AA Cuts at: 4318 6128 4318 Size: 1810 5514 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7324 BstXI CCAn_nnnn'nTGG Cuts at: 2732 2732 Size: 7324 BstYI r'GATC_y Cuts at: 1972 4654 5021 5406 5423 6875 6961 6973 7319 Size: 2682 367 385 17 1452 86 12 346 Cuts at: 7319 1972 Size: 1977 Fragments arranged by size: 2682 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 4531 4586 513 Size: 552 3466 55 3251 Fragments arranged by size: 3466 3251 552 55 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2045 2357 487 Size: 683 56 819 312 5454 Fragments arranged by size: 5454 819 683 312 56 BtsI GCAGTG_nn' Cuts at: 2806 3421 4570 4840 5634 5654 2806 Size: 615 1149 270 794 20 4476 Fragments arranged by size: 4476 1149 794 615 270 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7324 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2736 2771 4546 4581 529 Size: 35 2172 35 1775 35 3272 Fragments arranged by size: 3272 2172 1775 35 35 35 DraI TTT'AAA Cuts at: 3498 4628 3498 Size: 1130 6194 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2447 6331 809 Size: 507 1131 3884 1802 Fragments arranged by size: 3884 1802 1131 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3150 4771 5660 Size: 54 1425 50 275 18 1320 1621 889 Cuts at: 5660 7289 8 Size: 1629 43 Fragments arranged by size: 1629 1621 1425 1320 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4771 1537 Size: 275 2959 4090 Fragments arranged by size: 4090 2959 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2478 4273 5254 1353 Size: 1125 1795 981 3423 Fragments arranged by size: 3423 1795 1125 981 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2001 2096 5911 6285 6431 387 Size: 1064 138 412 95 3815 374 146 1280 Fragments arranged by size: 3815 1280 1064 412 374 146 138 95 EcoICRI GAG'CTC Cuts at: 2083 2083 Size: 7324 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2450 2976 3249 3312 3365 3509 3923 Size: 393 818 526 273 63 53 144 414 Cuts at: 3923 3932 5387 5984 6771 7039 1239 Size: 9 1455 597 787 268 1524 Fragments arranged by size: 1524 1455 818 787 597 526 414 393 273 268 144 63 53 9 EcoNI CCTnn'n_nnAGG Cuts at: 3098 3098 Size: 7324 EcoO109I rG'GnC_Cy Cuts at: 2071 2071 Size: 7324 EcoRI G'AATT_C Cuts at: 3286 4633 5011 3286 Size: 1347 378 5599 Fragments arranged by size: 5599 1347 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4133 4344 4615 4133 Size: 211 271 6842 Fragments arranged by size: 6842 271 211 FspI TGC'GCA Cuts at: 5830 5830 Size: 7324 HaeII r_GCGC'y Cuts at: 1678 6471 1678 Size: 4793 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2133 2165 2218 2250 2512 Size: 32 135 32 561 32 53 32 262 Cuts at: 2512 2544 3684 3716 3835 3867 5965 5997 6039 Size: 32 1140 32 119 32 2098 32 42 Cuts at: 6039 6071 1373 Size: 32 2626 Fragments arranged by size: 2626 2098 1140 561 262 135 119 53 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2334 4552 4668 4914 669 Size: 988 281 396 2218 116 246 3079 Fragments arranged by size: 3079 2218 988 396 281 246 116 HindIII A'AGCT_T Cuts at: 748 3278 4341 748 Size: 2530 1063 3731 Fragments arranged by size: 3731 2530 1063 HpaI GTT'AAC Cuts at: 2334 4914 2334 Size: 2580 4744 KpnI G_GTAC'C Cuts at: 4649 4649 Size: 7324 MfeI C'AATT_G Cuts at: 3722 4923 3722 Size: 1201 6123 MluI A'CGCG_T Cuts at: 7200 7200 Size: 7324 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4008 4068 6438 6622 1305 Size: 2703 60 2370 184 2007 Fragments arranged by size: 2703 2370 2007 184 60 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5502 Fragments arranged by size: 5502 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3602 5282 5641 5800 518 Size: 648 2436 1680 359 159 2042 Fragments arranged by size: 2436 2042 1680 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3470 4740 5417 6565 6810 1606 Size: 1864 1270 677 1148 245 2120 Fragments arranged by size: 2120 1864 1270 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6772 NdeI CA'TA_TG Cuts at: 387 387 Size: 7324 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7324 NotI GC'GGCC_GC Cuts at: 4771 4771 Size: 7324 NsiI A_TGCA'T Cuts at: 2010 2392 2461 3659 2010 Size: 382 69 1198 5675 Fragments arranged by size: 5675 1198 382 69 NspI r_CATG'y Cuts at: 1132 1229 2312 3551 4682 6227 1132 Size: 97 1083 1239 1131 1545 2229 Fragments arranged by size: 2229 1545 1239 1131 1083 97 PasI CC'CwG_GG Cuts at: 1348 2725 1348 Size: 1377 5947 PciI A'CATG_T Cuts at: 3547 6223 3547 Size: 2676 4648 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7324 PfoI T'CCnGG_A Cuts at: 4258 4258 Size: 7324 PmeI GTTT'AAAC Cuts at: 4628 4628 Size: 7324 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5393 5425 6906 6938 5393 Size: 32 1481 32 5779 Fragments arranged by size: 5779 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2071 2071 Size: 7324 PshAI GACnn'nnGTC Cuts at: 1613 2865 1613 Size: 1252 6072 PsiI TTA'TAA Cuts at: 3024 3141 3672 4894 3024 Size: 117 531 1222 5454 Fragments arranged by size: 5454 1222 531 117 PstI C_TGCA'G Cuts at: 830 2079 2367 3490 4676 830 Size: 1249 288 1123 1186 3478 Fragments arranged by size: 3478 1249 1186 1123 288 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7324 PvuII CAG'CTG Cuts at: 3470 3470 Size: 7324 RsrII CG'GwC_CG Cuts at: 2104 2104 Size: 7324 SacI G_AGCT'C Cuts at: 2085 2085 Size: 7324 SalI G'TCGA_C Cuts at: 1936 4666 1936 Size: 2730 4594 SbfI CC_TGCA'GG Cuts at: 2079 4676 2079 Size: 2597 4727 ScaI AGT'ACT Cuts at: 4525 5572 7284 4525 Size: 1047 1712 4565 Fragments arranged by size: 4565 1712 1047 SfcI C'TryA_G Cuts at: 826 1045 2075 2363 3486 4672 5807 6488 6679 Size: 219 1030 288 1123 1186 1135 681 191 Cuts at: 6679 826 Size: 1471 Fragments arranged by size: 1471 1186 1135 1123 1030 681 288 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7324 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7324 SmaI CCC'GGG Cuts at: 1872 4651 1872 Size: 2779 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2117 3004 5428 6329 6591 820 Size: 197 926 174 887 2424 901 262 1553 Fragments arranged by size: 2424 1553 926 901 887 262 197 174 SnaBI TAC'GTA Cuts at: 493 5115 493 Size: 4622 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7324 SphI G_CATG'C Cuts at: 1132 4682 1132 Size: 3550 3774 SspI AAT'ATT Cuts at: 5 52 3818 5248 5 Size: 47 3766 1430 2081 Fragments arranged by size: 3766 2081 1430 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7324 StyI C'CwwG_G Cuts at: 513 1065 2212 2541 3730 4763 513 Size: 552 1147 329 1189 1033 3074 Fragments arranged by size: 3074 1189 1147 1033 552 329 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5351 5368 5521 5706 6151 325 Size: 1255 3771 17 153 185 445 1498 Fragments arranged by size: 3771 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3035 4523 5570 Size: 274 80 33 51 1443 1058 1488 1047 Cuts at: 5570 7282 96 Size: 1712 138 Fragments arranged by size: 1712 1488 1443 1058 1047 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2208 2240 3075 3107 3118 3150 7284 7316 2208 Size: 32 835 32 11 32 4134 32 2216 Fragments arranged by size: 4134 2216 835 32 32 32 32 11 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7324 XbaI T'CTAG_A Cuts at: 4660 4660 Size: 7324 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7324 XmaI C'CCGG_G Cuts at: 1870 4649 1870 Size: 2779 4545 XmnI GAAnn'nnTTC Cuts at: 2472 2643 2928 4116 4432 5453 2472 Size: 171 285 1188 316 1021 4343 Fragments arranged by size: 4343 1188 1021 316 285 171 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5132 276 Size: 53 83 186 936 3598 2468 Fragments arranged by size: 3598 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI