Restriction map for
pFN21AA1598
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccII AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BcnI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRV EcoT22I FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 1130 TCCGGA T^CCGGA
AciI 392 CCGC CCGC(-3/-1)
AclI 285 AACGTT AA^CGTT
AlwNI 922 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1627 GGGCCC GGGCC^C
Asp700I 157 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 762 GAGCTC G[AT]GC[AT]^C
BalI 314 TGGCCA TGG^CCA
BanI 388 GGTGCC G^G[CT][GA]CC
BciVI 1537 GTATCC GTATCC(6/5)
BclI 774 TGATCA T^GATCA
BglII 1209 AGATCT A^GATCT
BsaJI 1658 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 1130 TCCGGA [AT]^CCGG[AT]
BseAI 1130 TCCGGA T^CCGGA
BsgI 1331 GTGCAG GTGCAG(16/14)
BsiHKAI 762 GAGCTC G[AT]GC[AT]^C
Bsp1407I 308 TGTACA T^GTACA
BspEI 1130 TCCGGA T^CCGGA
BsrGI 308 TGTACA T^GTACA
BstAPI 1685 GCATTGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EaeI 314 TGGCCA [CT]^GGCC[GA]
EcoNI 1612 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1658 CCAAGG C^C[AT][AT]GG
FbaI 774 TGATCA T^GATCA
HapII 1131 CCGG C^CGG
HgaI 1443 GCGTC GACGC(5/10)
HpaII 1131 CCGG C^CGG
MluNI 314 TGGCCA TGG^CCA
MroI 1130 TCCGGA T^CCGGA
MscI 314 TGGCCA TGG^CCA
MspA1I 25 CAGCTG C[AC]G^C[GT]G
MspI 1131 CCGG C^CGG
NspI 302 GCATGT [GA]CATG^[CT]
PpuMI 674 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 399 GACTTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 285 AACGTT AA^CGTT
PvuII 25 CAGCTG CAG^CTG
SacI 762 GAGCTC GAGCT^C
SapI 871 GAAGAGC GCTCTTC(1/4)
SexAI 1781 ACCAGGT A^CC[AT]GGT
SmlI 502 CTCAAG C^T[CT][GA]AG
SpeI 1378 ACTAGT A^CTAGT
SspBI 308 TGTACA T^GTACA
StyI 1658 CCAAGG C^C[AT][AT]GG
XbaI 936 TCTAGA T^CTAGA
XcmI 1517 CCATGCCAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 157 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaII 325 GGACC G^G[AT]CC
675 GGACC
BanII 762 GAGCTC G[GA]GC[CT]^C
1627 GGGCCC
BmyI 762 GAGCTC G[AGT]GC[ACT]^C
1627 GGGCCC
Bsp1286I 762 GAGCTC G[AGT]GC[ACT]^C
1627 GGGCCC
BstBI 680 TTCGAA TT^CGAA
1314 TTCGAA
BstXI 485 CCATTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1143 CCACCCCAGTGG
Cac8I 54 GCAAGC GC[ACGT]^[ACGT]GC
1221 GCAAGC
DraI 564 TTTAAA TTT^AAA
999 TTTAAA
HaeIII 315 GGCC GG^CC
1628 GGCC
NspV 680 TTCGAA TT^CGAA
1314 TTCGAA
PleI 694 GAGTC GAGTC(4/5)
1507 GAGTC
PstI 28 CTGCAG CTGCA^G
757 CTGCAG
SfuI 680 TTCGAA TT^CGAA
1314 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbsI BbvI BfaI BpmI BpuAI
BseRI BsiYI BslI BsmAI BsmFI
BsrI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRI EcoRII Fnu4HI FokI
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII