(Circular) (Six-base) MAPSORT of: pFN21AA1598.seq Check: 6776 from: 1 to: 6598 pFN21AA1598 6598 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4408 278 Size: 53 83 186 936 2872 2468 Fragments arranged by size: 2872 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3941 1937 Size: 2004 4594 Acc65I G'GTAC_C Cuts at: 3919 3919 Size: 6598 AclI AA'CG_TT Cuts at: 1563 2291 4725 5098 1563 Size: 728 2434 373 3063 Fragments arranged by size: 3063 2434 728 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2072 2274 2367 3006 3222 4661 6045 6313 Size: 440 202 93 639 216 1439 1384 268 Cuts at: 6313 1632 Size: 1917 Fragments arranged by size: 1917 1439 1384 639 440 268 216 202 93 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6401 AflIII A'CryG_T Cuts at: 1203 5497 6474 1203 Size: 4294 977 1327 Fragments arranged by size: 4294 1327 977 AgeI A'CCGG_T Cuts at: 5429 5429 Size: 6598 AhdI GACnn_n'nnGTC Cuts at: 5327 5327 Size: 6598 AleI CACnn'nnGTG Cuts at: 1166 3153 1166 Size: 1987 4611 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6598 AlwNI CAG_nnn'CTG Cuts at: 2932 4341 5913 2932 Size: 1409 1572 3617 Fragments arranged by size: 3617 1572 1409 ApaI G_GGCC'C Cuts at: 3636 3636 Size: 6598 ApaLI G'TGCA_C Cuts at: 4655 5811 4655 Size: 1156 5442 ApoI r'AATT_y Cuts at: 1493 2165 2175 2572 2844 2980 3001 3052 3590 Size: 672 10 397 272 136 21 51 538 Cuts at: 3590 3834 3907 4133 4285 6394 1493 Size: 244 73 226 152 2109 1697 Fragments arranged by size: 2109 1697 672 538 397 272 244 226 152 136 73 51 21 10 AseI AT'TA_AT Cuts at: 160 1032 5152 160 Size: 872 4120 1606 Fragments arranged by size: 4120 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 AvaI C'yCGr_G Cuts at: 1870 1943 3923 1870 Size: 73 1980 4545 Fragments arranged by size: 4545 1980 73 BamHI G'GATC_C Cuts at: 3928 4295 3928 Size: 367 6231 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2393 3919 5374 618 Size: 325 217 627 606 1526 1455 1842 Fragments arranged by size: 1842 1526 1455 627 606 325 217 BanII G_rGCy'C Cuts at: 1391 2771 3636 3917 1391 Size: 1380 865 281 4072 Fragments arranged by size: 4072 1380 865 281 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2085 2240 3308 3861 928 Size: 971 186 155 1068 553 3665 Fragments arranged by size: 3665 1068 971 553 186 155 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4789 4823 1903 Size: 34 2852 34 3678 Fragments arranged by size: 3678 2852 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3553 4490 5700 3553 Size: 937 1210 4451 Fragments arranged by size: 4451 1210 937 BclI T'GATC_A Cuts at: 1548 2779 1548 Size: 1231 5367 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5209 136 Size: 107 122 71 631 605 3537 1525 Fragments arranged by size: 3537 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3214 6593 3214 Size: 3379 3219 BlpI GC'TnA_GC Cuts at: 4015 4015 Size: 6598 Bme1580I G_kGCm'C Cuts at: 1790 3636 4659 5815 1790 Size: 1846 1023 1156 2573 Fragments arranged by size: 2573 1846 1156 1023 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6598 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3147 3371 3501 Size: 416 53 83 399 24 1730 224 130 Cuts at: 3501 3678 4329 5287 442 Size: 177 651 958 1753 Fragments arranged by size: 1753 1730 958 651 416 399 224 177 130 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6598 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2687 2693 2954 3011 3602 5258 1239 Size: 1448 6 261 57 591 1656 2579 Fragments arranged by size: 2579 1656 1448 591 261 57 6 Bpu10I CC'TnA_GC Cuts at: 3618 3618 Size: 6598 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2492 4723 5588 5886 2492 Size: 2231 865 298 3204 Fragments arranged by size: 3204 2231 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5261 882 Size: 617 3762 2219 Fragments arranged by size: 3762 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4389 493 Size: 711 3185 2702 Fragments arranged by size: 3185 2702 711 BsaBI GATnn'nnATC Cuts at: 6592 6592 Size: 6598 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4405 4787 5422 275 Size: 53 83 186 936 2872 382 635 1451 Fragments arranged by size: 2872 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3135 5031 5429 5703 5850 6261 3135 Size: 1896 398 274 147 411 3472 Fragments arranged by size: 3472 1896 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6373 Fragments arranged by size: 6373 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2663 3063 3066 1186 Size: 1477 400 3 4718 Fragments arranged by size: 4718 1477 400 3 BseYI C'CCAG_C Cuts at: 2326 2823 5801 6297 2326 Size: 497 2978 496 2627 Fragments arranged by size: 2978 2627 497 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3357 1163 Size: 573 1621 4404 Fragments arranged by size: 4404 1621 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4048 4809 5837 1540 Size: 275 186 2047 761 1028 2301 Fragments arranged by size: 2301 2047 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2771 4659 4744 5815 2771 Size: 1888 85 1071 3554 Fragments arranged by size: 3554 1888 1071 85 BsmI GAATG_Cn' Cuts at: 4109 4202 4109 Size: 93 6505 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2771 3636 3917 4659 4744 5815 1391 Size: 399 981 865 281 742 85 1071 2174 Fragments arranged by size: 2174 1071 981 865 742 399 281 85 BspEI T'CCGG_A Cuts at: 3135 6261 3135 Size: 3126 3472 BspHI T'CATG_A Cuts at: 4487 6217 4487 Size: 1730 4868 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3953 844 Size: 3109 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4485 1394 Size: 429 2662 3507 Fragments arranged by size: 3507 2662 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5093 5267 65 Size: 1183 3845 174 1396 Fragments arranged by size: 3845 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5242 5429 1700 Size: 110 3432 187 2869 Fragments arranged by size: 3432 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2313 96 Size: 2217 4381 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6598 BssSI C'ACGA_G Cuts at: 4658 5670 4658 Size: 1012 5586 BstAPI GCAn_nnn'nTGC Cuts at: 794 3696 794 Size: 2902 3696 BstBI TT'CG_AA Cuts at: 2686 3320 5402 2686 Size: 634 2082 3882 Fragments arranged by size: 3882 2082 634 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6598 BstXI CCAn_nnnn'nTGG Cuts at: 2497 3155 2497 Size: 658 5940 BstYI r'GATC_y Cuts at: 1972 3214 3751 3802 3928 4295 4680 4697 6149 Size: 1242 537 51 126 367 385 17 1452 Cuts at: 6149 6235 6247 6593 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1242 537 385 367 346 126 86 51 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6046 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5859 Fragments arranged by size: 5859 683 56 BtsI GCAGTG_nn' Cuts at: 3166 3345 4114 4908 4928 3166 Size: 179 769 794 20 4836 Fragments arranged by size: 4836 794 769 179 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6598 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6563 DraI TTT'AAA Cuts at: 2571 3006 3902 2571 Size: 435 896 5267 Fragments arranged by size: 5267 896 435 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5605 809 Size: 507 4289 1802 Fragments arranged by size: 4289 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2319 4045 4934 Size: 54 1425 50 275 18 489 1726 889 Cuts at: 4934 6563 8 Size: 1629 43 Fragments arranged by size: 1726 1629 1425 889 489 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4045 1537 Size: 275 2233 4090 Fragments arranged by size: 4090 2233 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2016 2343 2634 2832 2871 2982 3231 4528 Size: 663 327 291 198 39 111 249 1297 Cuts at: 4528 1353 Size: 3423 Fragments arranged by size: 3423 1297 663 327 291 249 198 111 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5185 5559 5705 387 Size: 1064 138 3596 374 146 1280 Fragments arranged by size: 3596 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2769 2769 Size: 6598 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2274 2367 2687 2693 2954 3006 Size: 393 440 202 93 320 6 261 52 Cuts at: 3006 3011 3222 3602 4661 5258 6045 6313 1239 Size: 5 211 380 1059 597 787 268 1524 Fragments arranged by size: 1524 1059 787 597 440 393 380 320 268 261 211 202 93 52 6 5 EcoNI CCTnn'n_nnAGG Cuts at: 3621 3621 Size: 6598 EcoO109I rG'GnC_Cy Cuts at: 2680 3632 3633 2680 Size: 952 1 5645 Fragments arranged by size: 5645 952 1 EcoRI G'AATT_C Cuts at: 2844 2980 3052 3590 3907 4285 2844 Size: 136 72 538 317 378 5157 Fragments arranged by size: 5157 538 378 317 136 72 FspI TGC'GCA Cuts at: 5104 5104 Size: 6598 HaeII r_GCGC'y Cuts at: 1678 5745 1678 Size: 4067 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3433 3465 3503 3535 5239 Size: 32 135 32 1861 32 38 32 1704 Cuts at: 5239 5271 5313 5345 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1861 1704 135 42 38 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3942 4188 669 Size: 988 281 2004 246 3079 Fragments arranged by size: 3079 2004 988 281 246 HindIII A'AGCT_T Cuts at: 748 2256 2796 3394 748 Size: 1508 540 598 3952 Fragments arranged by size: 3952 1508 598 540 HpaI GTT'AAC Cuts at: 4188 4188 Size: 6598 KpnI G_GTAC'C Cuts at: 3923 3923 Size: 6598 MfeI C'AATT_G Cuts at: 4197 4197 Size: 6598 MluI A'CGCG_T Cuts at: 6474 6474 Size: 6598 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3512 3830 3915 5712 5896 1305 Size: 2207 318 85 1797 184 2007 Fragments arranged by size: 2207 2007 1797 318 184 85 MscI TGG'CCA Cuts at: 10 64 1489 1832 2321 10 Size: 54 1425 343 489 4287 Fragments arranged by size: 4287 1425 489 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3128 3153 3527 4556 4915 5074 518 Size: 648 1962 25 374 1029 359 159 2042 Fragments arranged by size: 2042 1962 1029 648 374 359 159 25 MspA1I CmG'CkG Cuts at: 1606 2032 4014 4691 5839 6084 1606 Size: 426 1982 677 1148 245 2120 Fragments arranged by size: 2120 1982 1148 677 426 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6046 NdeI CA'TA_TG Cuts at: 387 387 Size: 6598 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6598 NotI GC'GGCC_GC Cuts at: 4045 4045 Size: 6598 NspI r_CATG'y Cuts at: 1132 1229 2311 3956 5501 1132 Size: 97 1082 1645 1545 2229 Fragments arranged by size: 2229 1645 1545 1082 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6598 PciI A'CATG_T Cuts at: 5497 5497 Size: 6598 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6598 PmeI GTTT'AAAC Cuts at: 3902 3902 Size: 6598 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4667 4699 6180 6212 4667 Size: 32 1481 32 5053 Fragments arranged by size: 5053 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2680 2680 Size: 6598 PshAI GACnn'nnGTC Cuts at: 1613 2408 1613 Size: 795 5803 PsiI TTA'TAA Cuts at: 4168 4168 Size: 6598 PspOMI G'GGCC_C Cuts at: 3632 3632 Size: 6598 PstI C_TGCA'G Cuts at: 830 2037 2766 3950 830 Size: 1207 729 1184 3478 Fragments arranged by size: 3478 1207 1184 729 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6598 PvuII CAG'CTG Cuts at: 2032 2032 Size: 6598 SacI G_AGCT'C Cuts at: 2771 2771 Size: 6598 SalI G'TCGA_C Cuts at: 1936 3940 1936 Size: 2004 4594 SapI GCTCTTCn'nnn_ Cuts at: 2871 2871 Size: 6598 SbfI CC_TGCA'GG Cuts at: 3950 3950 Size: 6598 ScaI AGT'ACT Cuts at: 4846 6558 4846 Size: 1712 4886 SexAI A'CCwGG_T Cuts at: 3786 3786 Size: 6598 SfcI C'TryA_G Cuts at: 826 1045 2033 2379 2634 2762 3946 5081 5762 Size: 219 988 346 255 128 1184 1135 681 Cuts at: 5762 5953 826 Size: 191 1471 Fragments arranged by size: 1471 1184 1135 988 681 346 255 219 191 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6598 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6598 SmaI CCC'GGG Cuts at: 1872 3925 1872 Size: 2053 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2507 4702 5603 5865 820 Size: 197 926 564 2195 901 262 1553 Fragments arranged by size: 2195 1553 926 901 564 262 197 SnaBI TAC'GTA Cuts at: 493 4389 493 Size: 3896 2702 SpeI A'CTAG_T Cuts at: 152 3383 152 Size: 3231 3367 SphI G_CATG'C Cuts at: 1132 3956 1132 Size: 2824 3774 SspI AAT'ATT Cuts at: 5 52 4522 5 Size: 47 4470 2081 Fragments arranged by size: 4470 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6598 StyI C'CwwG_G Cuts at: 513 1065 3663 4037 513 Size: 552 2598 374 3074 Fragments arranged by size: 3074 2598 552 374 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2837 3313 4625 4642 4795 4980 5425 Size: 1255 1257 476 1312 17 153 185 445 Cuts at: 5425 325 Size: 1498 Fragments arranged by size: 1498 1312 1257 1255 476 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2313 2416 4844 Size: 274 80 33 51 1443 336 103 2428 Cuts at: 4844 6556 96 Size: 1712 138 Fragments arranged by size: 2428 1712 1443 336 274 138 103 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6558 6590 6558 Size: 32 6566 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6598 XbaI T'CTAG_A Cuts at: 2941 3934 2941 Size: 993 5605 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3529 3529 Size: 6598 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6598 XmaI C'CCGG_G Cuts at: 1870 3923 1870 Size: 2053 4545 XmnI GAAnn'nnTTC Cuts at: 2166 4727 2166 Size: 2561 4037 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4406 276 Size: 53 83 186 936 2872 2468 Fragments arranged by size: 2872 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI