Restriction map for
pFN21AA1598
(Linear) (Six-base) MAPSORT of: KIAA1598.seq Check: 1968 from: 1 to: 1893
KIAA1598.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AclI AA'CG_TT
Cuts at: 0 286 1893
Size: 286 1607
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 269 362 1001 1217 1893
Size: 67 202 93 639 216 676
Fragments arranged by size:
676 639 216 202 93 67
AleI CACnn'nnGTG
Cuts at: 0 1148 1893
Size: 1148 745
AlwNI CAG_nnn'CTG
Cuts at: 0 927 1893
Size: 927 966
ApaI G_GGCC'C
Cuts at: 0 1631 1893
Size: 1631 262
ApoI r'AATT_y
Cuts at: 0 160 170 567 839 975 996 1047 1585
Size: 160 10 397 272 136 21 51 538
Cuts at: 1585 1829 1893
Size: 244 64
Fragments arranged by size:
538 397 272 244 160 136 64 51
21 10
BanI G'GyrC_C
Cuts at: 0 388 1893
Size: 388 1505
BanII G_rGCy'C
Cuts at: 0 766 1631 1893
Size: 766 865 262
Fragments arranged by size:
865 766 262
BbsI GAAGACnn'nnnn_
Cuts at: 0 80 235 1303 1856 1893
Size: 80 155 1068 553 37
Fragments arranged by size:
1068 553 155 80 37
BciVI GTATCCnnnnn_n'
Cuts at: 0 1548 1893
Size: 1548 345
BclI T'GATC_A
Cuts at: 0 774 1893
Size: 774 1119
BglII A'GATC_T
Cuts at: 0 1209 1893
Size: 1209 684
Bme1580I G_kGCm'C
Cuts at: 0 1631 1893
Size: 1631 262
BmrI ACTGGGnnnn_n'
Cuts at: 0 1142 1366 1496 1673 1893
Size: 1142 224 130 177 220
Fragments arranged by size:
1142 224 220 177 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 688 949 1006 1597 1893
Size: 682 6 261 57 591 296
Fragments arranged by size:
682 591 296 261 57 6
Bpu10I CC'TnA_GC
Cuts at: 0 1613 1893
Size: 1613 280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 1893
Size: 487 1406
BsaWI w'CCGG_w
Cuts at: 0 1130 1893
Size: 1130 763
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 658 1058 1061 1893
Size: 658 400 3 832
Fragments arranged by size:
832 658 400 3
BseYI C'CCAG_C
Cuts at: 0 321 818 1893
Size: 321 497 1075
Fragments arranged by size:
1075 497 321
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1352 1893
Size: 1352 541
BsiHKAI G_wGCw'C
Cuts at: 0 766 1893
Size: 766 1127
Bsp1286I G_dGCh'C
Cuts at: 0 766 1631 1893
Size: 766 865 262
Fragments arranged by size:
865 766 262
BspEI T'CCGG_A
Cuts at: 0 1130 1893
Size: 1130 763
BsrGI T'GTAC_A
Cuts at: 0 308 1893
Size: 308 1585
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1691 1893
Size: 1691 202
BstBI TT'CG_AA
Cuts at: 0 681 1315 1893
Size: 681 634 578
Fragments arranged by size:
681 634 578
BstXI CCAn_nnnn'nTGG
Cuts at: 0 492 1150 1893
Size: 492 658 743
Fragments arranged by size:
743 658 492
BstYI r'GATC_y
Cuts at: 0 1209 1746 1797 1893
Size: 1209 537 51 96
Fragments arranged by size:
1209 537 96 51
BtsI GCAGTG_nn'
Cuts at: 0 1161 1340 1893
Size: 1161 179 553
Fragments arranged by size:
1161 553 179
DraI TTT'AAA
Cuts at: 0 566 1001 1893
Size: 566 435 892
Fragments arranged by size:
892 566 435
EaeI y'GGCC_r
Cuts at: 0 314 1893
Size: 314 1579
EarI CTCTTCn'nnn_
Cuts at: 0 11 338 629 827 866 977 1226 1893
Size: 11 327 291 198 39 111 249 667
Fragments arranged by size:
667 327 291 249 198 111 39 11
EcoICRI GAG'CTC
Cuts at: 0 764 1893
Size: 764 1129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 269 362 682 688 949 1001 1006
Size: 67 202 93 320 6 261 52 5
Cuts at: 1006 1217 1597 1893
Size: 211 380 296
Fragments arranged by size:
380 320 296 261 211 202 93 67
52 6 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1616 1893
Size: 1616 277
EcoO109I rG'GnC_Cy
Cuts at: 0 675 1627 1628 1893
Size: 675 952 1 265
Fragments arranged by size:
952 675 265 1
EcoRI G'AATT_C
Cuts at: 0 839 975 1047 1585 1893
Size: 839 136 72 538 308
Fragments arranged by size:
839 538 308 136 72
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1428 1460 1498 1530 1893
Size: 1428 32 38 32 363
Fragments arranged by size:
1428 363 38 32 32
HindIII A'AGCT_T
Cuts at: 0 251 791 1389 1893
Size: 251 540 598 504
Fragments arranged by size:
598 540 504 251
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1507 1825 1893
Size: 1507 318 68
Fragments arranged by size:
1507 318 68
MscI TGG'CCA
Cuts at: 0 316 1893
Size: 316 1577
MslI CAynn'nnrTG
Cuts at: 0 1123 1148 1522 1893
Size: 1123 25 374 371
Fragments arranged by size:
1123 374 371 25
MspA1I CmG'CkG
Cuts at: 0 27 1893
Size: 27 1866
NspI r_CATG'y
Cuts at: 0 306 1893
Size: 306 1587
PpuMI rG'GwC_Cy
Cuts at: 0 675 1893
Size: 675 1218
PshAI GACnn'nnGTC
Cuts at: 0 403 1893
Size: 403 1490
PspOMI G'GGCC_C
Cuts at: 0 1627 1893
Size: 1627 266
PstI C_TGCA'G
Cuts at: 0 32 761 1893
Size: 32 729 1132
Fragments arranged by size:
1132 729 32
PvuII CAG'CTG
Cuts at: 0 27 1893
Size: 27 1866
SacI G_AGCT'C
Cuts at: 0 766 1893
Size: 766 1127
SapI GCTCTTCn'nnn_
Cuts at: 0 866 1893
Size: 866 1027
SexAI A'CCwGG_T
Cuts at: 0 1781 1893
Size: 1781 112
SfcI C'TryA_G
Cuts at: 0 28 374 629 757 1893
Size: 28 346 255 128 1136
Fragments arranged by size:
1136 346 255 128 28
SmlI C'TyrA_G
Cuts at: 0 502 1893
Size: 502 1391
SpeI A'CTAG_T
Cuts at: 0 1378 1893
Size: 1378 515
StyI C'CwwG_G
Cuts at: 0 1658 1893
Size: 1658 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 832 1308 1893
Size: 832 476 585
Fragments arranged by size:
832 585 476
TatI w'GTAC_w
Cuts at: 0 308 411 1893
Size: 308 103 1482
Fragments arranged by size:
1482 308 103
XbaI T'CTAG_A
Cuts at: 0 936 1893
Size: 936 957
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1524 1893
Size: 1524 369
XmnI GAAnn'nnTTC
Cuts at: 0 161 1893
Size: 161 1732
Enzymes that do cut:
AclI AcuI AleI AlwNI ApaI ApoI BanI BanII
BbsI BciVI BclI BglII Bme1580I BmrI BpmI Bpu10I
BpuEI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI
BsrGI BstAPI BstBI BstXI BstYI BtsI DraI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HindIII
MmeI MscI MslI MspA1I NspI PpuMI PshAI PspOMI
PstI PvuII SacI SapI SexAI SfcI SmlI SpeI
StyI TaqII TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI BglI
BlpI BmgBI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsaXI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoRV
FalI FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI
SwaI TstI Tth111I XhoI XmaI ZraI