Restriction map for
pFN21AA1592
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspI BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BsaAI BsaBI BsaWI
BseAI BsiWI BspDI BspEI BspMI
BsrDI BssHII Bst1107I BstAPI BstUI
BstZ17I ClaI CpoI DraI Eco47III
EcoRI EcoT22I FbaI FseI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MseI MspA1I MunI
MvnI NdeI NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SexAI SfiI SgrAI SnaBI
SpeI SphI StuI SwaI Tru9I
Tth111I Van91I XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AatII 1582 GACGTC GACGT^C
AcsI 487 AAATTC [GA]^AATT[CT]
AflIII 1106 ACATGT A^C[GA][CT]GT
AhdI 805 GACGAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 430 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 427 CCTCAGG CC^T[ACGT]AGG
ApoI 487 AAATTC [GA]^AATT[CT]
AspEI 805 GACGAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 209 TGCGCA TGC^GCA
AvrII 309 CCTAGG C^CTAGG
BalI 983 TGGCCA TGG^CCA
BbeI 1468 GGCGCC GGCGC^C
BlnI 309 CCTAGG C^CTAGG
BlpI 1550 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1550 GCTCAGC GC^T[ACGT]AGC
BsmI 1884 GAATGC GAATGC(1/-1)
BspLU11I 1106 ACATGT A^CATGT
BsrFI 1389 GCCGGC [GA]^CCGG[CT]
BstBI 799 TTCGAA TT^CGAA
BstXI 1778 CCACCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 427 CCTCAGG CC^T[ACGT]AGG
CelII 1550 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1389 GCCGGC [GA]^CCGG[CT]
DraIII 907 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1657 GACTTCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 200 CCATGG C^C[GA][CT]GG
EagI 1391 CGGCCG C^GGCCG
Eam1105I 805 GACGAGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1391 CGGCCG C^GGCCG
Eco52I 1391 CGGCCG C^GGCCG
Eco81I 427 CCTCAGG CC^T[ACGT]AGG
EcoNI 423 CCTACCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 209 TGCGCA TGC^GCA
HgaI 1664 GCGTC GACGC(5/10)
HincII 325 GTCAAC GT[CT]^[GA]AC
HindII 325 GTCAAC GT[CT]^[GA]AC
KasI 1468 GGCGCC G^GCGCC
MluNI 983 TGGCCA TGG^CCA
MscI 983 TGGCCA TGG^CCA
NaeI 1389 GCCGGC GCC^GGC
NarI 1468 GGCGCC GG^CGCC
NcoI 200 CCATGG C^CATGG
NgoMIV 1389 GCCGGC G^CCGGC
NspI 1106 ACATGT [GA]CATG^[CT]
NspV 799 TTCGAA TT^CGAA
PpuMI 1034 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1500 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 75 GCTCTTC GCTCTTC(1/4)
SbfI 1413 CCTGCAGG CCTGCA^GG
ScaI 1352 AGTACT AGT^ACT
SfoI 1468 GGCGCC GGC^GCC
SfuI 799 TTCGAA TT^CGAA
SmaI 23 CCCGGG CCC^GGG
Sse8387I 1413 CCTGCAGG CCTGCA^GG
SspI 817 AATATT AAT^ATT
TfiI 561 GATTC G^A[AT]TC
XbaI 348 TCTAGA T^CTAGA
XmaCI 23 CCCGGG C^CCGGG
XmaI 23 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 1468 GGCGCC G[GA]^CG[CT]C
1582 GACGTC
ApaI 26 GGGCCC GGGCC^C
40 GGGCCC
AvaI 23 CCCGGG C^[CT]CG[GA]G
178 CTCGGG
BbsI 802 GAAGAC GAAGAC(2/6)
922 GTCTTC
BpuAI 802 GAAGAC GAAGAC(2/6)
922 GTCTTC
BsaHI 1468 GGCGCC G[GA]^CG[CT]C
1582 GACGTC
BsaI 1828 GAGACC GGTCTC(1/5)
1251 GGTCTC
BsiEI 1391 CGGCCG CG[GA][CT]^CG
1515 CGACCG
BsmBI 1584 CGTCTC CGTCTC(1/5)
1462 GAGACG
BsoBI 23 CCCGGG C^[CT]CG[GA]G
178 CTCGGG
Bsp1407I 1109 TGTACA T^GTACA
1373 TGTACA
BspHI 695 TCATGA T^CATGA
719 TCATGA
BsrBI 1226 CCGCTC CCGCTC(-3/-3)
1548 CCGCTC
BsrGI 1109 TGTACA T^GTACA
1373 TGTACA
BsrI 1620 CCAGT ACTGG(1/-1)
1644 CCAGT
BstEII 1018 GGTGACC G^GT[ACGT]ACC
1047 GGTCACC
BstPI 1018 GGTGACC G^GT[ACGT]ACC
1047 GGTCACC
EarI 76 CTCTTC CTCTTC(1/4)
1838 CTCTTC
EcoO65I 1018 GGTGACC G^GT[ACGT]ACC
1047 GGTCACC
EcoRV 691 GATATC GAT^ATC
1275 GATATC
HaeII 245 GGCGCT [GA]GCGC^[CT]
1468 GGCGCC
Hin1I 1468 GGCGCC G[GA]^CG[CT]C
1582 GACGTC
Ksp632I 76 CTCTTC CTCTTC(1/4)
1838 CTCTTC
PleI 67 GACTC GAGTC(4/5)
1099 GAGTC
PstI 1414 CTGCAG CTGCA^G
1592 CTGCAG
RcaI 695 TCATGA T^CATGA
719 TCATGA
SacI 640 GAGCTC GAGCT^C
1189 GAGCTC
SspBI 1109 TGTACA T^GTACA
1373 TGTACA
TaqI 800 TCGA T^CGA
1067 TCGA
Tsp509I 132 AATT ^AATT
488 AATT
TthHB8I 800 TCGA T^CGA
1067 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
ApaLI AspHI AvaII BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BssSI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TseI
Tsp45I TspRI XhoII