Restriction map for
pFN21AA1592
(Linear) (Six-base) MAPSORT of: KIAA1592.seq Check: 5653 from: 1 to: 1893
KIAA1592.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AatII G_ACGT'C
Cuts at: 0 1586 1893
Size: 1586 307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 1644 1893
Size: 70 1574 249
Fragments arranged by size:
1574 249 70
AflIII A'CryG_T
Cuts at: 0 1106 1893
Size: 1106 787
AhdI GACnn_n'nnGTC
Cuts at: 0 810 1893
Size: 810 1083
AlwNI CAG_nnn'CTG
Cuts at: 0 435 1893
Size: 435 1458
ApaI G_GGCC'C
Cuts at: 0 30 44 1893
Size: 30 14 1849
Fragments arranged by size:
1849 30 14
ApaLI G'TGCA_C
Cuts at: 0 18 913 1773 1893
Size: 18 895 860 120
Fragments arranged by size:
895 860 120 18
ApoI r'AATT_y
Cuts at: 0 487 1893
Size: 487 1406
AvaI C'yCGr_G
Cuts at: 0 23 178 1893
Size: 23 155 1715
Fragments arranged by size:
1715 155 23
AvrII C'CTAG_G
Cuts at: 0 309 1893
Size: 309 1584
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1291 1324 1893
Size: 1291 33 569
Fragments arranged by size:
1291 569 33
BanI G'GyrC_C
Cuts at: 0 229 463 658 1468 1893
Size: 229 234 195 810 425
Fragments arranged by size:
810 425 234 229 195
BanII G_rGCy'C
Cuts at: 0 30 44 269 617 644 1193 1893
Size: 30 14 225 348 27 549 700
Fragments arranged by size:
700 549 348 225 30 27 14
BbeI G_GCGC'C
Cuts at: 0 1472 1893
Size: 1472 421
BbsI GAAGACnn'nnnn_
Cuts at: 0 809 915 1893
Size: 809 106 978
Fragments arranged by size:
978 809 106
BglI GCCn_nnn'nGGC
Cuts at: 0 39 114 273 1893
Size: 39 75 159 1620
Fragments arranged by size:
1620 159 75 39
BlpI GC'TnA_GC
Cuts at: 0 1551 1893
Size: 1551 342
Bme1580I G_kGCm'C
Cuts at: 0 22 30 44 663 917 1674 1777 1893
Size: 22 8 14 619 254 757 103 116
Fragments arranged by size:
757 619 254 116 103 22 14 8
BmgBI CAC'GTC
Cuts at: 0 1062 1893
Size: 1062 831
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1872 1893
Size: 1872 21
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 973 1075 1893
Size: 691 282 102 818
Fragments arranged by size:
818 691 282 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 865 1288 1315 1893
Size: 865 423 27 578
Fragments arranged by size:
865 578 423 27
BsaI GGTCTCn'nnnn_
Cuts at: 0 1257 1822 1893
Size: 1257 565 71
Fragments arranged by size:
1257 565 71
BsaHI Gr'CG_yC
Cuts at: 0 1469 1583 1893
Size: 1469 114 310
Fragments arranged by size:
1469 310 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1858 1888 1893
Size: 1858 30 5
Fragments arranged by size:
1858 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 378 652 970 1084 1207 1893
Size: 103 275 274 318 114 123 686
Fragments arranged by size:
686 318 275 274 123 114 103
BseYI C'CCAG_C
Cuts at: 0 29 701 1524 1893
Size: 29 672 823 369
Fragments arranged by size:
823 672 369 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 238 855 1012 1849 1893
Size: 103 135 617 157 837 44
Fragments arranged by size:
837 617 157 135 103 44
BsiEI CG_ry'CG
Cuts at: 0 1394 1518 1893
Size: 1394 124 375
Fragments arranged by size:
1394 375 124
BsiHKAI G_wGCw'C
Cuts at: 0 22 442 644 917 1193 1346 1777 1893
Size: 22 420 202 273 276 153 431 116
Fragments arranged by size:
431 420 276 273 202 153 116 22
BsmI GAATG_Cn'
Cuts at: 0 1890 1893
Size: 1890 3
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1456 1590 1893
Size: 1456 134 303
Fragments arranged by size:
1456 303 134
Bsp1286I G_dGCh'C
Cuts at: 0 22 30 44 269 442 617 644 663
Size: 22 8 14 225 173 175 27 19
Cuts at: 663 917 1193 1346 1674 1777 1893
Size: 254 276 153 328 103 116
Fragments arranged by size:
328 276 254 225 175 173 153 116
103 27 22 19 14 8
BspHI T'CATG_A
Cuts at: 0 695 719 1893
Size: 695 24 1174
Fragments arranged by size:
1174 695 24
BsrBI CCG'CTC
Cuts at: 0 1228 1550 1893
Size: 1228 322 343
Fragments arranged by size:
1228 343 322
BsrFI r'CCGG_y
Cuts at: 0 1389 1893
Size: 1389 504
BsrGI T'GTAC_A
Cuts at: 0 1109 1373 1893
Size: 1109 264 520
Fragments arranged by size:
1109 520 264
BssSI C'ACGA_G
Cuts at: 0 628 1675 1879 1893
Size: 628 1047 204 14
Fragments arranged by size:
1047 628 204 14
BstBI TT'CG_AA
Cuts at: 0 800 1893
Size: 800 1093
BstEII G'GTnAC_C
Cuts at: 0 1018 1047 1893
Size: 1018 29 846
Fragments arranged by size:
1018 846 29
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1785 1893
Size: 1785 108
BstYI r'GATC_y
Cuts at: 0 419 1088 1286 1893
Size: 419 669 198 607
Fragments arranged by size:
669 607 419 198
Bsu36I CC'TnA_GG
Cuts at: 0 428 1893
Size: 428 1465
BtgI C'CryG_G
Cuts at: 0 200 1893
Size: 200 1693
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 746 1216 1893
Size: 746 470 677
Fragments arranged by size:
746 677 470
BtsI GCAGTG_nn'
Cuts at: 0 70 1893
Size: 70 1823
DraIII CAC_nnn'GTG
Cuts at: 0 912 1893
Size: 912 981
DrdI GACnn_nn'nnGTC
Cuts at: 0 1663 1893
Size: 1663 230
EaeI y'GGCC_r
Cuts at: 0 983 1223 1391 1796 1893
Size: 983 240 168 405 97
Fragments arranged by size:
983 405 240 168 97
EagI C'GGCC_G
Cuts at: 0 1391 1893
Size: 1391 502
EarI CTCTTCn'nnn_
Cuts at: 0 82 1844 1893
Size: 82 1762 49
Fragments arranged by size:
1762 82 49
EcoICRI GAG'CTC
Cuts at: 0 642 1191 1893
Size: 642 549 702
Fragments arranged by size:
702 642 549
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 691 973 1075 1644 1893
Size: 70 621 282 102 569 249
Fragments arranged by size:
621 569 282 249 102 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 427 1893
Size: 427 1466
EcoO109I rG'GnC_Cy
Cuts at: 0 41 432 1035 1893
Size: 41 391 603 858
Fragments arranged by size:
858 603 391 41
EcoRV GAT'ATC
Cuts at: 0 693 1277 1893
Size: 693 584 616
Fragments arranged by size:
693 616 584
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1176 1893
Size: 1176 717
FspI TGC'GCA
Cuts at: 0 211 1893
Size: 211 1682
HaeII r_GCGC'y
Cuts at: 0 249 1472 1893
Size: 249 1223 421
Fragments arranged by size:
1223 421 249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 52 84 411 443 796 828 876 908
Size: 52 32 327 32 353 32 48 32
Cuts at: 908 1821 1853 1893
Size: 913 32 40
Fragments arranged by size:
913 353 327 52 48 40 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 327 1893
Size: 327 1566
KasI G'GCGC_C
Cuts at: 0 1468 1893
Size: 1468 425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 740 920 1064 1127 1538 1893
Size: 740 180 144 63 411 355
Fragments arranged by size:
740 411 355 180 144 63
MscI TGG'CCA
Cuts at: 0 985 1893
Size: 985 908
MslI CAynn'nnrTG
Cuts at: 0 379 793 1783 1883 1893
Size: 379 414 990 100 10
Fragments arranged by size:
990 414 379 100 10
NaeI GCC'GGC
Cuts at: 0 1391 1893
Size: 1391 502
NarI GG'CG_CC
Cuts at: 0 1469 1893
Size: 1469 424
NcoI C'CATG_G
Cuts at: 0 200 1893
Size: 200 1693
NgoMIV G'CCGG_C
Cuts at: 0 1389 1893
Size: 1389 504
NspI r_CATG'y
Cuts at: 0 1110 1893
Size: 1110 783
PciI A'CATG_T
Cuts at: 0 1106 1893
Size: 1106 787
PfoI T'CCnGG_A
Cuts at: 0 536 707 740 1091 1893
Size: 536 171 33 351 802
Fragments arranged by size:
802 536 351 171 33
PpuMI rG'GwC_Cy
Cuts at: 0 1035 1893
Size: 1035 858
PshAI GACnn'nnGTC
Cuts at: 0 1504 1893
Size: 1504 389
PspOMI G'GGCC_C
Cuts at: 0 26 40 1893
Size: 26 14 1853
Fragments arranged by size:
1853 26 14
PstI C_TGCA'G
Cuts at: 0 1418 1596 1893
Size: 1418 178 297
Fragments arranged by size:
1418 297 178
SacI G_AGCT'C
Cuts at: 0 644 1193 1893
Size: 644 549 700
Fragments arranged by size:
700 644 549
SapI GCTCTTCn'nnn_
Cuts at: 0 82 1893
Size: 82 1811
SbfI CC_TGCA'GG
Cuts at: 0 1418 1893
Size: 1418 475
ScaI AGT'ACT
Cuts at: 0 1354 1893
Size: 1354 539
SfcI C'TryA_G
Cuts at: 0 683 1414 1592 1893
Size: 683 731 178 301
Fragments arranged by size:
731 683 301 178
SfoI GGC'GCC
Cuts at: 0 1470 1893
Size: 1470 423
SmaI CCC'GGG
Cuts at: 0 25 1893
Size: 25 1868
SmlI C'TyrA_G
Cuts at: 0 880 1303 1330 1893
Size: 880 423 27 563
Fragments arranged by size:
880 563 423 27
SspI AAT'ATT
Cuts at: 0 819 1893
Size: 819 1074
StyI C'CwwG_G
Cuts at: 0 200 309 1601 1893
Size: 200 109 1292 292
Fragments arranged by size:
1292 292 200 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 451 1494 1795 1893
Size: 451 1043 301 98
Fragments arranged by size:
1043 451 301 98
TatI w'GTAC_w
Cuts at: 0 1109 1352 1373 1646 1688 1893
Size: 1109 243 21 273 42 205
Fragments arranged by size:
1109 273 243 205 42 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1858 1890 1893
Size: 1858 32 3
Fragments arranged by size:
1858 32 3
XbaI T'CTAG_A
Cuts at: 0 348 1893
Size: 348 1545
XmaI C'CCGG_G
Cuts at: 0 23 1893
Size: 23 1870
ZraI GAC'GTC
Cuts at: 0 1584 1893
Size: 1584 309
Enzymes that do cut:
AatII AcuI AflIII AhdI AlwNI ApaI ApaLI ApoI
AvaI AvrII BaeI BanI BanII BbeI BbsI BglI
BlpI Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BsrBI BsrFI BsrGI BssSI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV
FalI FspI HaeII Hin4I HincII KasI MmeI MscI
MslI NaeI NarI NcoI NgoMIV NspI PciI PfoI
PpuMI PshAI PspOMI PstI SacI SapI SbfI ScaI
SfcI SfoI SmaI SmlI SspI StyI TaqII TatI
TstI XbaI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI AscI AseI AsiSI BamHI BbvCI BcgI
BciVI BclI BfrBI BglII BmrI BmtI Bpu10I BsaAI
BsaBI BsaWI BsiWI BspEI BspMI BsrDI BssHII BstAPI
BstZ17I ClaI CspCI DraI EciI EcoRI FseI FspAI
HindIII HpaI KpnI MfeI MluI MspA1I NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmeI PmlI
PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XcmI XhoI XmnI