(Circular) (Six-base) MAPSORT of: pFN21AA1582.seq Check: 7401 from: 1 to: 9775 pFN21AA1582 9775 bp With 182 enzymes: SgfI * October 20, 2009 10:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2676 3553 2676 Size: 877 8898 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4796 6231 7585 278 Size: 53 83 186 936 3260 1435 1354 2468 Fragments arranged by size: 3260 2468 1435 1354 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7118 1937 Size: 5181 4594 Acc65I G'GTAC_C Cuts at: 4093 4184 7096 4093 Size: 91 2912 6772 Fragments arranged by size: 6772 2912 91 AclI AA'CG_TT Cuts at: 1563 5491 7902 8275 1563 Size: 3928 2411 373 3063 Fragments arranged by size: 3928 3063 2411 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2748 3501 5141 7838 9222 9490 1632 Size: 1116 753 1640 2697 1384 268 1917 Fragments arranged by size: 2697 1917 1640 1384 1116 753 268 AfeI AGC'GCT Cuts at: 4924 4924 Size: 9775 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9578 AflIII A'CryG_T Cuts at: 1203 4233 4701 6714 8674 9651 1203 Size: 3030 468 2013 1960 977 1327 Fragments arranged by size: 3030 2013 1960 1327 977 468 AgeI A'CCGG_T Cuts at: 4096 5849 8606 4096 Size: 1753 2757 5265 Fragments arranged by size: 5265 2757 1753 AhdI GACnn_n'nnGTC Cuts at: 8504 8504 Size: 9775 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9775 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2882 3393 5583 5706 6956 1184 Size: 1698 511 2190 123 1250 4003 Fragments arranged by size: 4003 2190 1698 1250 511 123 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3473 3505 3473 Size: 32 9743 AlwNI CAG_nnn'CTG Cuts at: 3524 3887 3924 4749 5161 5632 6546 6799 7518 Size: 363 37 825 412 471 914 253 719 Cuts at: 7518 9090 3524 Size: 1572 4209 Fragments arranged by size: 4209 1572 914 825 719 471 412 363 253 37 ApaI G_GGCC'C Cuts at: 6986 6986 Size: 9775 ApaLI G'TGCA_C Cuts at: 5142 7832 8988 5142 Size: 2690 1156 5929 Fragments arranged by size: 5929 2690 1156 ApoI r'AATT_y Cuts at: 1493 3386 5200 6167 7084 7310 7462 9571 1493 Size: 1893 1814 967 917 226 152 2109 1697 Fragments arranged by size: 2109 1893 1814 1697 967 917 226 152 AseI AT'TA_AT Cuts at: 160 1032 2292 5433 8329 160 Size: 872 1260 3141 2896 1606 Fragments arranged by size: 3141 2896 1606 1260 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9775 AvaI C'yCGr_G Cuts at: 1870 1943 3604 4861 6502 7100 1870 Size: 73 1661 1257 1641 598 4545 Fragments arranged by size: 4545 1661 1641 1257 598 73 AvrII C'CTAG_G Cuts at: 3401 3401 Size: 9775 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4654 4687 4654 Size: 33 9742 BamHI G'GATC_C Cuts at: 2852 5540 7105 7472 2852 Size: 2688 1565 367 5155 Fragments arranged by size: 5155 2688 1565 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 4093 4184 6394 6467 Size: 325 217 627 282 2024 91 2210 73 Cuts at: 6467 6955 7096 8551 618 Size: 488 141 1455 1842 Fragments arranged by size: 2210 2024 1842 1455 627 488 325 282 217 141 91 73 BanII G_rGCy'C Cuts at: 1391 5088 6986 7094 1391 Size: 3697 1898 108 4072 Fragments arranged by size: 4072 3697 1898 108 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2057 4796 5025 5766 928 Size: 971 158 2739 229 741 4937 Fragments arranged by size: 4937 2739 971 741 229 158 BbvCI CC'TCA_GC Cuts at: 4189 4597 4875 4189 Size: 408 278 9089 Fragments arranged by size: 9089 408 278 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3792 3826 5682 5716 6532 6566 7966 Size: 34 1855 34 1856 34 816 34 1400 Cuts at: 7966 8000 1903 Size: 34 3678 Fragments arranged by size: 3678 1856 1855 1400 816 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7667 8877 7667 Size: 1210 8565 BclI T'GATC_A Cuts at: 1548 4833 4909 1548 Size: 3285 76 6414 Fragments arranged by size: 6414 3285 76 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3167 6480 6921 Size: 107 122 71 631 605 1495 3313 441 Cuts at: 6921 6981 8386 136 Size: 60 1405 1525 Fragments arranged by size: 3313 1525 1495 1405 631 605 441 122 107 71 60 BglII A'GATC_T Cuts at: 3908 9770 3908 Size: 5862 3913 BlpI GC'TnA_GC Cuts at: 6851 7058 7192 6851 Size: 207 134 9434 Fragments arranged by size: 9434 207 134 Bme1580I G_kGCm'C Cuts at: 1790 2023 2113 3297 5146 6399 6960 6986 7836 Size: 233 90 1184 1849 1253 561 26 850 Cuts at: 7836 8992 1790 Size: 1156 2573 Fragments arranged by size: 2573 1849 1253 1184 1156 850 561 233 90 26 BmgBI CAC'GTC Cuts at: 1318 4702 4948 6336 1318 Size: 3384 246 1388 4757 Fragments arranged by size: 4757 3384 1388 246 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2635 3246 3757 Size: 416 53 83 399 24 1218 611 511 Cuts at: 3757 3976 4012 4048 5145 5316 5872 5878 5893 Size: 219 36 36 1097 171 556 6 15 Cuts at: 5893 5928 6261 6955 7506 8464 442 Size: 35 333 694 551 958 1753 Fragments arranged by size: 1753 1218 1097 958 694 611 556 551 511 416 399 333 219 171 83 53 36 36 35 24 15 6 BmtI G_CTAG'C Cuts at: 1056 4011 1056 Size: 2955 6820 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6444 6444 Size: 9775 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2161 3799 5972 5991 6006 6414 6535 6952 Size: 922 1638 2173 19 15 408 121 417 Cuts at: 6952 8435 1239 Size: 1483 2579 Fragments arranged by size: 2579 2173 1638 1483 922 417 408 121 19 15 Bpu10I CC'TnA_GC Cuts at: 4189 4597 4875 4189 Size: 408 278 9089 Fragments arranged by size: 9089 408 278 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2691 5319 6284 6633 7900 8765 9063 2691 Size: 2628 965 349 1267 865 298 3403 Fragments arranged by size: 3403 2628 1267 965 865 349 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5408 8438 882 Size: 617 3909 3030 2219 Fragments arranged by size: 3909 3030 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6110 7566 493 Size: 711 4906 1456 2702 Fragments arranged by size: 4906 2702 1456 711 BsaBI GATnn'nnATC Cuts at: 9769 9769 Size: 9775 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4334 4793 6228 7582 Size: 53 83 186 936 2801 459 1435 1354 Cuts at: 7582 7964 8599 275 Size: 382 635 1451 Fragments arranged by size: 2801 1451 1435 1354 936 635 459 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4096 5537 5849 6666 8208 8606 8880 9027 9438 Size: 1441 312 817 1542 398 274 147 411 Cuts at: 9438 4096 Size: 4433 Fragments arranged by size: 4433 1542 1441 817 411 398 312 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2805 2835 3137 3167 3689 Size: 30 165 30 1402 30 302 30 522 Cuts at: 3689 3719 5776 5806 5900 5930 1178 Size: 30 2057 30 94 30 5023 Fragments arranged by size: 5023 2057 1402 522 302 165 94 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4059 4726 4892 6287 6445 6490 6698 1186 Size: 2873 667 166 1395 158 45 208 4263 Fragments arranged by size: 4263 2873 1395 667 208 166 158 45 BseYI C'CCAG_C Cuts at: 2262 2320 2996 3924 4131 4410 4517 4638 4963 Size: 58 676 928 207 279 107 121 325 Cuts at: 4963 5182 5316 5523 6989 8978 9474 2262 Size: 219 134 207 1466 1989 496 2563 Fragments arranged by size: 2563 1989 1466 928 676 496 325 279 219 207 207 134 121 107 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3468 3804 5318 5397 5666 6396 6695 Size: 573 1732 336 1514 79 269 730 299 Cuts at: 6695 1163 Size: 4243 Fragments arranged by size: 4243 1732 1514 730 573 336 299 269 79 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7225 7986 9014 1540 Size: 275 186 5224 761 1028 2301 Fragments arranged by size: 5224 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2483 5146 5335 6521 7836 7921 8992 2483 Size: 2663 189 1186 1315 85 1071 3266 Fragments arranged by size: 3266 2663 1315 1186 1071 189 85 BsmI GAATG_Cn' Cuts at: 3052 7286 7379 3052 Size: 4234 93 5448 Fragments arranged by size: 5448 4234 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4325 4325 Size: 9775 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2113 2483 3297 5088 5146 5335 Size: 399 233 90 370 814 1791 58 189 Cuts at: 5335 6399 6521 6960 6986 7094 7836 7921 8992 Size: 1064 122 439 26 108 742 85 1071 Cuts at: 8992 1391 Size: 2174 Fragments arranged by size: 2174 1791 1071 1064 814 742 439 399 370 233 189 122 108 90 85 58 26 BspEI T'CCGG_A Cuts at: 5537 9438 5537 Size: 3901 5874 BspHI T'CATG_A Cuts at: 3495 4821 6379 7664 9394 3495 Size: 1326 1558 1285 1730 3876 Fragments arranged by size: 3876 1730 1558 1326 1285 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2676 3553 5760 7062 7130 844 Size: 1832 877 2207 1302 68 3489 Fragments arranged by size: 3489 2207 1832 1302 877 68 BsrBI CCG'CTC Cuts at: 1394 1823 7662 1394 Size: 429 5839 3507 Fragments arranged by size: 5839 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2607 6661 8270 8444 65 Size: 1183 1359 4054 1609 174 1396 Fragments arranged by size: 4054 1609 1396 1359 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4090 4096 4558 4827 5849 6039 8419 Size: 110 2280 6 462 269 1022 190 2380 Cuts at: 8419 8606 1700 Size: 187 2869 Fragments arranged by size: 2869 2380 2280 1022 462 269 190 187 110 6 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9775 BssHII G'CGCG_C Cuts at: 1925 5570 1925 Size: 3645 6130 BssSI C'ACGA_G Cuts at: 3904 4306 5075 5636 6418 7835 8847 3904 Size: 402 769 561 782 1417 1012 4832 Fragments arranged by size: 4832 1417 1012 782 769 561 402 BstAPI GCAn_nnn'nTGC Cuts at: 794 2837 4589 794 Size: 2043 1752 5980 Fragments arranged by size: 5980 2043 1752 BstBI TT'CG_AA Cuts at: 6559 8579 6559 Size: 2020 7755 BstEII G'GTnAC_C Cuts at: 1184 2765 1184 Size: 1581 8194 BstXI CCAn_nnnn'nTGG Cuts at: 2328 5920 6581 2328 Size: 3592 661 5522 Fragments arranged by size: 5522 3592 661 BstYI r'GATC_y Cuts at: 1972 2852 3851 3908 5540 5598 7105 7472 7857 Size: 880 999 57 1632 58 1507 367 385 Cuts at: 7857 7874 9326 9412 9424 9770 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1632 1507 1452 999 880 385 367 346 86 58 57 17 12 Bsu36I CC'TnA_GG Cuts at: 5358 6529 5358 Size: 1171 8604 BtgI C'CryG_G Cuts at: 513 1065 2004 2355 5037 5706 5919 6185 6845 Size: 552 939 351 2682 669 213 266 660 Cuts at: 6845 6871 513 Size: 26 3417 Fragments arranged by size: 3417 2682 939 669 660 552 351 266 213 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9036 Fragments arranged by size: 9036 683 56 BtsI GCAGTG_nn' Cuts at: 2194 2728 2866 2953 3375 4123 4528 4651 6247 Size: 534 138 87 422 748 405 123 1596 Cuts at: 6247 6590 6940 7291 8085 8105 2194 Size: 343 350 351 794 20 3864 Fragments arranged by size: 3864 1596 794 748 534 422 405 351 350 343 138 123 87 20 ClaI AT'CG_AT Cuts at: 1554 4369 1554 Size: 2815 6960 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5038 5073 529 Size: 35 4474 35 5231 Fragments arranged by size: 5231 4474 35 35 DraI TTT'AAA Cuts at: 3981 7079 3981 Size: 3098 6677 DraIII CAC_nnn'GTG Cuts at: 5141 5141 Size: 9775 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6227 8782 809 Size: 507 4911 2555 1802 Fragments arranged by size: 4911 2555 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 3667 4989 Size: 54 1425 50 275 18 318 1519 1322 Cuts at: 4989 5703 6037 6744 6895 7222 8111 9740 8 Size: 714 334 707 151 327 889 1629 43 Fragments arranged by size: 1629 1519 1425 1322 889 714 707 334 327 318 275 151 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 7222 1537 Size: 275 5410 4090 Fragments arranged by size: 5410 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2584 3520 4567 4883 5076 5317 7705 1353 Size: 1231 936 1047 316 193 241 2388 3423 Fragments arranged by size: 3423 2388 1231 1047 936 316 241 193 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8362 8736 8882 387 Size: 1064 138 6773 374 146 1280 Fragments arranged by size: 6773 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2161 2748 3501 3799 5141 5972 5991 Size: 393 529 587 753 298 1342 831 19 Cuts at: 5991 6006 6414 6535 6952 7838 8435 9222 9490 Size: 15 408 121 417 886 597 787 268 Cuts at: 9490 1239 Size: 1524 Fragments arranged by size: 1524 1342 886 831 787 753 597 587 529 417 408 393 298 268 121 19 15 EcoNI CCTnn'n_nnAGG Cuts at: 2775 2938 2775 Size: 163 9612 EcoO109I rG'GnC_Cy Cuts at: 2906 4201 5158 5629 5869 6982 7051 2906 Size: 1295 957 471 240 1113 69 5630 Fragments arranged by size: 5630 1295 1113 957 471 240 69 EcoRI G'AATT_C Cuts at: 5200 6167 7084 7462 5200 Size: 967 917 378 7513 Fragments arranged by size: 7513 967 917 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5390 5390 Size: 9775 FspI TGC'GCA Cuts at: 8281 8281 Size: 9775 HaeII r_GCGC'y Cuts at: 1678 3596 4926 5617 6800 6996 8922 1678 Size: 1918 1330 691 1183 196 1926 2531 Fragments arranged by size: 2531 1926 1918 1330 1183 691 196 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3689 3721 3797 3829 4172 Size: 32 135 32 2117 32 76 32 343 Cuts at: 4172 4204 5212 5244 5756 5788 6119 6151 6314 Size: 32 1008 32 512 32 331 32 163 Cuts at: 6314 6346 8416 8448 8490 8522 1373 Size: 32 2070 32 42 32 2626 Fragments arranged by size: 2626 2117 2070 1008 512 343 331 163 135 76 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3535 5833 6286 6438 7119 7365 Size: 988 281 1597 2298 453 152 681 246 Cuts at: 7365 669 Size: 3079 Fragments arranged by size: 3079 2298 1597 988 681 453 281 246 152 HindIII A'AGCT_T Cuts at: 748 748 Size: 9775 HpaI GTT'AAC Cuts at: 6438 7365 6438 Size: 927 8848 KpnI G_GTAC'C Cuts at: 4097 4188 7100 4097 Size: 91 2912 6772 Fragments arranged by size: 6772 2912 91 MfeI C'AATT_G Cuts at: 7374 7374 Size: 9775 MluI A'CGCG_T Cuts at: 9651 9651 Size: 9775 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2298 3081 3872 8889 9073 1305 Size: 993 783 791 5017 184 2007 Fragments arranged by size: 5017 2007 993 791 783 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3669 6897 10 Size: 54 1425 343 1837 3228 2888 Fragments arranged by size: 3228 2888 1837 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4850 5843 7733 8092 8251 518 Size: 648 3684 993 1890 359 159 2042 Fragments arranged by size: 3684 2042 1890 993 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2324 3551 4363 4530 4874 5473 5479 5620 Size: 718 1227 812 167 344 599 6 141 Cuts at: 5620 6546 6816 6847 6860 7062 7191 7868 9016 Size: 926 270 31 13 202 129 677 1148 Cuts at: 9016 9261 1606 Size: 245 2120 Fragments arranged by size: 2120 1227 1148 926 812 718 677 599 344 270 245 202 167 141 129 31 13 6 NaeI GCC'GGC Cuts at: 4829 4829 Size: 9775 NcoI C'CATG_G Cuts at: 513 1065 2004 5706 5919 6185 6871 513 Size: 552 939 3702 213 266 686 3417 Fragments arranged by size: 3702 3417 939 686 552 266 213 NdeI CA'TA_TG Cuts at: 387 5445 387 Size: 5058 4717 NgoMIV G'CCGG_C Cuts at: 4827 4827 Size: 9775 NheI G'CTAG_C Cuts at: 1052 4007 1052 Size: 2955 6820 NotI GC'GGCC_GC Cuts at: 7222 7222 Size: 9775 NspI r_CATG'y Cuts at: 1132 1229 4237 5191 6718 7133 8678 1132 Size: 97 3008 954 1527 415 1545 2229 Fragments arranged by size: 3008 2229 1545 1527 954 415 97 PasI CC'CwG_GG Cuts at: 1348 2023 5161 1348 Size: 675 3138 5962 Fragments arranged by size: 5962 3138 675 PciI A'CATG_T Cuts at: 4233 6714 8674 4233 Size: 2481 1960 5334 Fragments arranged by size: 5334 2481 1960 PflMI CCAn_nnn'nTGG Cuts at: 1108 2157 4680 6546 6877 1108 Size: 1049 2523 1866 331 4006 Fragments arranged by size: 4006 2523 1866 1049 331 PfoI T'CCnGG_A Cuts at: 2920 6175 6276 2920 Size: 3255 101 6419 Fragments arranged by size: 6419 3255 101 PmeI GTTT'AAAC Cuts at: 7079 7079 Size: 9775 PmlI CAC'GTG Cuts at: 6110 6110 Size: 9775 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7844 7876 9357 9389 7844 Size: 32 1481 32 8230 Fragments arranged by size: 8230 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2906 4201 5869 7051 2906 Size: 1295 1668 1182 5630 Fragments arranged by size: 5630 1668 1295 1182 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9775 PsiI TTA'TAA Cuts at: 7345 7345 Size: 9775 PspOMI G'GGCC_C Cuts at: 6982 6982 Size: 9775 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3193 3225 3193 Size: 32 9743 PstI C_TGCA'G Cuts at: 830 4745 5478 5592 5695 5757 6201 7127 830 Size: 3915 733 114 103 62 444 926 3478 Fragments arranged by size: 3915 3478 926 733 444 114 103 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9775 PvuII CAG'CTG Cuts at: 2324 4363 4874 5473 5479 5620 6546 6816 2324 Size: 2039 511 599 6 141 926 270 5283 Fragments arranged by size: 5283 2039 926 599 511 270 141 6 SacII CC_GC'GG Cuts at: 6848 6848 Size: 9775 SalI G'TCGA_C Cuts at: 1936 7117 1936 Size: 5181 4594 SanDI GG'GwC_CC Cuts at: 4201 4201 Size: 9775 SapI GCTCTTCn'nnn_ Cuts at: 4567 5076 4567 Size: 509 9266 SbfI CC_TGCA'GG Cuts at: 7127 7127 Size: 9775 ScaI AGT'ACT Cuts at: 3646 6409 6965 8023 9735 3646 Size: 2763 556 1058 1712 3686 Fragments arranged by size: 3686 2763 1712 1058 556 SfcI C'TryA_G Cuts at: 826 1045 2010 3183 4741 5474 5588 5691 5753 Size: 219 965 1173 1558 733 114 103 62 Cuts at: 5753 6197 7123 8258 8939 9130 826 Size: 444 926 1135 681 191 1471 Fragments arranged by size: 1558 1471 1173 1135 965 926 733 681 444 219 191 114 103 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9775 SgrAI Cr'CCGG_yG Cuts at: 1700 4558 1700 Size: 2858 6917 SmaI CCC'GGG Cuts at: 1872 3606 7102 1872 Size: 1734 3496 4545 Fragments arranged by size: 4545 3496 1734 SmlI C'TyrA_G Cuts at: 820 1017 1943 2706 5334 6299 6648 7879 8780 Size: 197 926 763 2628 965 349 1231 901 Cuts at: 8780 9042 820 Size: 262 1553 Fragments arranged by size: 2628 1553 1231 965 926 901 763 349 262 197 SnaBI TAC'GTA Cuts at: 493 7566 493 Size: 7073 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9775 SphI G_CATG'C Cuts at: 1132 5191 7133 1132 Size: 4059 1942 3774 Fragments arranged by size: 4059 3774 1942 SspI AAT'ATT Cuts at: 5 52 6205 7699 5 Size: 47 6153 1494 2081 Fragments arranged by size: 6153 2081 1494 47 StuI AGG'CCT Cuts at: 1517 2676 3437 4814 4883 1517 Size: 1159 761 1377 69 6409 Fragments arranged by size: 6409 1377 1159 761 69 StyI C'CwwG_G Cuts at: 513 1065 2004 2113 3289 3308 3401 3820 5706 Size: 552 939 109 1176 19 93 419 1886 Cuts at: 5706 5919 6185 6678 6693 6787 6871 7214 513 Size: 213 266 493 15 94 84 343 3074 Fragments arranged by size: 3074 1886 1176 939 552 493 419 343 266 213 109 94 93 84 19 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2599 3516 3724 4531 4988 5153 5742 Size: 1255 1019 917 208 807 457 165 589 Cuts at: 5742 5794 6430 6820 6908 7802 7819 7972 8157 Size: 52 636 390 88 894 17 153 185 Cuts at: 8157 8602 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1019 917 894 807 636 589 457 445 390 208 185 165 153 88 52 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2335 3503 3644 Size: 274 80 33 51 1443 358 1168 141 Cuts at: 3644 6407 6963 8021 9733 96 Size: 2763 556 1058 1712 138 Fragments arranged by size: 2763 1712 1443 1168 1058 556 358 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2805 2837 3137 3169 5776 5808 5900 5932 9735 Size: 32 300 32 2607 32 92 32 3803 Cuts at: 9735 9767 2805 Size: 32 2813 Fragments arranged by size: 3803 2813 2607 300 92 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6337 1316 Size: 5021 4754 XbaI T'CTAG_A Cuts at: 5207 7111 5207 Size: 1904 7871 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2185 3677 3963 5158 2185 Size: 1492 286 1195 6802 Fragments arranged by size: 6802 1492 1195 286 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9775 XmaI C'CCGG_G Cuts at: 1870 3604 7100 1870 Size: 1734 3496 4545 Fragments arranged by size: 4545 3496 1734 XmnI GAAnn'nnTTC Cuts at: 6776 7904 6776 Size: 1128 8647 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4794 6229 7583 276 Size: 53 83 186 936 3260 1435 1354 2468 Fragments arranged by size: 3260 2468 1435 1354 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BbeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI RsrII SacI SexAI SfiI SfoI SrfI SwaI