Restriction map for
pFN21AA1575
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# NO CUT ENZYMES ( 80 enzymes) #
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AcsI AflII AgeI AhdI Alw44I
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI BbrPI
BclI BfrI BsaBI BsaI BsiWI
BspHI BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstZ17I CpoI DraI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HpaI MamI
MfeI MluI MseI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI RsrII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmlI SpeI Sse8387I SspI SwaI
Tru9I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1303 GACGTC GACGT^C
Acc65I 2656 GGTACC G^GTACC
AocI 1987 CCTGAGG CC^T[ACGT]AGG
Asp718I 2656 GGTACC G^GTACC
AviII 844 TGCGCA TGC^GCA
AvrII 2673 CCTAGG C^CTAGG
BalI 2493 TGGCCA TGG^CCA
BamHI 2757 GGATCC G^GATCC
BbeI 1846 GGCGCC GGCGC^C
BbvCI 667 CCTCAGC CCTCAGC(-5/-2)
BciVI 1176 GTATCC GTATCC(6/5)
BglI 2106 GCCCTATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1492 AGATCT A^GATCT
BlnI 2673 CCTAGG C^CTAGG
BsmBI 408 CGTCTC CGTCTC(1/5)
BsmI 1933 GCATTC GAATGC(1/-1)
Bsp1407I 650 TGTACA T^GTACA
BspDI 190 ATCGAT AT^CGAT
BspLU11I 647 ACATGT A^CATGT
BsrGI 650 TGTACA T^GTACA
BstXI 1388 CCACGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1987 CCTGAGG CC^T[ACGT]AGG
ClaI 190 ATCGAT AT^CGAT
DrdI 2401 GACAAGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1987 CCTGAGG CC^T[ACGT]AGG
FspI 844 TGCGCA TGC^GCA
HindIII 2404 AAGCTT A^AGCTT
KasI 1846 GGCGCC G^GCGCC
KpnI 2656 GGTACC GGTAC^C
KspI 1151 CCGCGG CCGC^GG
MluNI 2493 TGGCCA TGG^CCA
MscI 2493 TGGCCA TGG^CCA
NaeI 2529 GCCGGC GCC^GGC
NarI 1846 GGCGCC GG^CGCC
NgoMIV 2529 GCCGGC G^CCGGC
PshAI 2385 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 36 CAGCTG CAG^CTG
SacI 1755 GAGCTC GAGCT^C
SacII 1151 CCGCGG CCGC^GG
SfoI 1846 GGCGCC GGC^GCC
SnaBI 2413 TACGTA TAC^GTA
SphI 1224 GCATGC GCATG^C
SspBI 650 TGTACA T^GTACA
Tsp509I 1950 AATT ^AATT
XcmI 183 CCATAGGATCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 1152 CGCG CG^CG
1634 CGCG
AclI 28 AACGTT AA^CGTT
1666 AACGTT
AfaI 651 GTAC GT^AC
2657 GTAC
AflIII 647 ACATGT A^C[GA][CT]GT
2459 ACGTGT
AlwNI 691 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1982 CAGCCCCTG
BcgI 2721 CGATGGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2946 CGAACTGTATGC
BlpI 2503 GCTGAGC GC^T[ACGT]AGC
2970 GCTCAGC
Bpu1102I 2503 GCTGAGC GC^T[ACGT]AGC
2970 GCTCAGC
BsaAI 2413 TACGTA [CT]AC^GT[GA]
2914 TACGTG
BsgI 711 CTGCAC GTGCAG(16/14)
1688 GTGCAG
BsiEI 1012 CGGCCG CG[GA][CT]^CG
2124 CGGCCG
BsrFI 2122 ACCGGC [GA]^CCGG[CT]
2529 GCCGGC
BstEII 960 GGTCACC G^GT[ACGT]ACC
2920 GGTGACC
BstPI 960 GGTCACC G^GT[ACGT]ACC
2920 GGTGACC
BstUI 1152 CGCG CG^CG
1634 CGCG
CelII 2503 GCTGAGC GC^T[ACGT]AGC
2970 GCTCAGC
Cfr10I 2122 ACCGGC [GA]^CCGG[CT]
2529 GCCGGC
DraIII 157 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
2789 CACCAAGTG
EagI 1012 CGGCCG C^GGCCG
2124 CGGCCG
EarI 139 GAAGAG CTCTTC(1/4)
1552 GAAGAG
EclXI 1012 CGGCCG C^GGCCG
2124 CGGCCG
Eco52I 1012 CGGCCG C^GGCCG
2124 CGGCCG
EcoNI 1955 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2562 CCTGAGAGAGG
EcoO65I 960 GGTCACC G^GT[ACGT]ACC
2920 GGTGACC
HgaI 109 GACGC GACGC(5/10)
875 GCGTC
Ksp632I 139 GAAGAG CTCTTC(1/4)
1552 GAAGAG
MslI 2326 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2454 CACAAACGTG
MvnI 1152 CGCG CG^CG
1634 CGCG
Psp1406I 28 AACGTT AA^CGTT
1666 AACGTT
PstI 1049 CTGCAG CTGCA^G
2536 CTGCAG
RsaI 651 GTAC GT^AC
2657 GTAC
StuI 508 AGGCCT AGG^CCT
656 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AccIII AciI AcyI AluI
AlwI Aor51HI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BsaWI BseAI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspEI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI Eco47III EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MroI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XhoII XmaCI
XmaI