Restriction map for
pFN21AA1575
(Linear) (Six-base) MAPSORT of: KIAA1575.seq Check: 1573 from: 1 to: 3012
KIAA1575.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2985 3012
Size: 2985 27
AatII G_ACGT'C
Cuts at: 0 1307 3012
Size: 1307 1705
AccI GT'mk_AC
Cuts at: 0 2036 2411 2417 3012
Size: 2036 375 6 595
Fragments arranged by size:
2036 595 375 6
Acc65I G'GTAC_C
Cuts at: 0 2656 3012
Size: 2656 356
AclI AA'CG_TT
Cuts at: 0 29 1667 3012
Size: 29 1638 1345
Fragments arranged by size:
1638 1345 29
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 1088 3012
Size: 417 671 1924
Fragments arranged by size:
1924 671 417
AfeI AGC'GCT
Cuts at: 0 494 727 2502 3012
Size: 494 233 1775 510
Fragments arranged by size:
1775 510 494 233
AflIII A'CryG_T
Cuts at: 0 647 2459 3012
Size: 647 1812 553
Fragments arranged by size:
1812 647 553
AleI CACnn'nnGTG
Cuts at: 0 2458 3012
Size: 2458 554
AlwNI CAG_nnn'CTG
Cuts at: 0 696 1987 3012
Size: 696 1291 1025
Fragments arranged by size:
1291 1025 696
AvaI C'yCGr_G
Cuts at: 0 252 385 1022 1219 1534 3012
Size: 252 133 637 197 315 1478
Fragments arranged by size:
1478 637 315 252 197 133
AvrII C'CTAG_G
Cuts at: 0 2673 3012
Size: 2673 339
BamHI G'GATC_C
Cuts at: 0 2757 3012
Size: 2757 255
BanI G'GyrC_C
Cuts at: 0 592 1204 1530 1645 1738 1846 2081 2656
Size: 592 612 326 115 93 108 235 575
Cuts at: 2656 3012
Size: 356
Fragments arranged by size:
612 592 575 356 326 235 115 108
93
BanII G_rGCy'C
Cuts at: 0 292 532 1759 2259 2510 3004 3012
Size: 292 240 1227 500 251 494 8
Fragments arranged by size:
1227 500 494 292 251 240 8
BbeI G_GCGC'C
Cuts at: 0 1850 3012
Size: 1850 1162
BbsI GAAGACnn'nnnn_
Cuts at: 0 86 1240 1322 2389 2616 3012
Size: 86 1154 82 1067 227 396
Fragments arranged by size:
1154 1067 396 227 86 82
BbvCI CC'TCA_GC
Cuts at: 0 668 3012
Size: 668 2344
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2710 2744 2935 2969 3012
Size: 2710 34 191 34 43
Fragments arranged by size:
2710 191 43 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1187 3012
Size: 1187 1825
BglI GCCn_nnn'nGGC
Cuts at: 0 2112 3012
Size: 2112 900
BglII A'GATC_T
Cuts at: 0 1492 3012
Size: 1492 1520
BlpI GC'TnA_GC
Cuts at: 0 2504 2971 3012
Size: 2504 467 41
Fragments arranged by size:
2504 467 41
Bme1580I G_kGCm'C
Cuts at: 0 1209 1220 1535 2084 2787 3012
Size: 1209 11 315 549 703 225
Fragments arranged by size:
1209 703 549 315 225 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 295 1608 2041 3012
Size: 295 1313 433 971
Fragments arranged by size:
1313 971 433 295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 1451 2722 3012
Size: 689 762 1271 290
Fragments arranged by size:
1271 762 689 290
Bpu10I CC'TnA_GC
Cuts at: 0 668 3012
Size: 668 2344
BsaAI yAC'GTr
Cuts at: 0 2415 2916 3012
Size: 2415 501 96
Fragments arranged by size:
2415 501 96
BsaHI Gr'CG_yC
Cuts at: 0 875 1304 1847 3012
Size: 875 429 543 1165
Fragments arranged by size:
1165 875 543 429
BsaWI w'CCGG_w
Cuts at: 0 1188 1432 2279 3012
Size: 1188 244 847 733
Fragments arranged by size:
1188 847 733 244
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1446 1476 3012
Size: 1446 30 1536
Fragments arranged by size:
1536 1446 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1262 1454 2233 2247 2584 3012
Size: 1262 192 779 14 337 428
Fragments arranged by size:
1262 779 428 337 192 14
BseYI C'CCAG_C
Cuts at: 0 495 689 819 1040 1208 1698 1980 2470
Size: 495 194 130 221 168 490 282 490
Cuts at: 2470 3012
Size: 542
Fragments arranged by size:
542 495 490 490 282 221 194 168
130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 696 1709 3012
Size: 696 1013 1303
Fragments arranged by size:
1303 1013 696
BsiEI CG_ry'CG
Cuts at: 0 1015 2127 3012
Size: 1015 1112 885
Fragments arranged by size:
1112 1015 885
BsiHKAI G_wGCw'C
Cuts at: 0 178 1430 1759 3012
Size: 178 1252 329 1253
Fragments arranged by size:
1253 1252 329 178
BsmI GAATG_Cn'
Cuts at: 0 1933 3012
Size: 1933 1079
BsmBI CGTCTCn'nnnn_
Cuts at: 0 414 3012
Size: 414 2598
Bsp1286I G_dGCh'C
Cuts at: 0 178 292 532 1209 1220 1430 1535 1759
Size: 178 114 240 677 11 210 105 224
Cuts at: 1759 2084 2259 2510 2787 3004 3012
Size: 325 175 251 277 217 8
Fragments arranged by size:
677 325 277 251 240 224 217 210
178 175 114 105 11 8
BspEI T'CCGG_A
Cuts at: 0 1188 1432 2279 3012
Size: 1188 244 847 733
Fragments arranged by size:
1188 847 733 244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 746 2644 2985 3012
Size: 746 1898 341 27
Fragments arranged by size:
1898 746 341 27
BsrDI GCAATG_nn'
Cuts at: 0 840 1994 2989 3012
Size: 840 1154 995 23
Fragments arranged by size:
1154 995 840 23
BsrFI r'CCGG_y
Cuts at: 0 2122 2529 3012
Size: 2122 407 483
Fragments arranged by size:
2122 483 407
BsrGI T'GTAC_A
Cuts at: 0 650 3012
Size: 650 2362
BstEII G'GTnAC_C
Cuts at: 0 960 2920 3012
Size: 960 1960 92
Fragments arranged by size:
1960 960 92
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1395 3012
Size: 1395 1617
BstYI r'GATC_y
Cuts at: 0 1088 1492 2236 2757 3012
Size: 1088 404 744 521 255
Fragments arranged by size:
1088 744 521 404 255
Bsu36I CC'TnA_GG
Cuts at: 0 1988 3012
Size: 1988 1024
BtgI C'CryG_G
Cuts at: 0 862 1151 1388 3012
Size: 862 289 237 1624
Fragments arranged by size:
1624 862 289 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2541 2735 3012
Size: 2541 194 277
Fragments arranged by size:
2541 277 194
BtsI GCAGTG_nn'
Cuts at: 0 1508 2787 3012
Size: 1508 1279 225
Fragments arranged by size:
1508 1279 225
ClaI AT'CG_AT
Cuts at: 0 191 3012
Size: 191 2821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1307 1342 3012
Size: 1307 35 1670
Fragments arranged by size:
1670 1307 35
DraIII CAC_nnn'GTG
Cuts at: 0 162 2794 3012
Size: 162 2632 218
Fragments arranged by size:
2632 218 162
DrdI GACnn_nn'nnGTC
Cuts at: 0 2407 3012
Size: 2407 605
EaeI y'GGCC_r
Cuts at: 0 1012 1154 1805 2124 2493 3012
Size: 1012 142 651 319 369 519
Fragments arranged by size:
1012 651 519 369 319 142
EagI C'GGCC_G
Cuts at: 0 1012 2124 3012
Size: 1012 1112 888
Fragments arranged by size:
1112 1012 888
EarI CTCTTCn'nnn_
Cuts at: 0 134 1547 3012
Size: 134 1413 1465
Fragments arranged by size:
1465 1413 134
EcoICRI GAG'CTC
Cuts at: 0 1757 3012
Size: 1757 1255
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 689 1088 1451 2722 3012
Size: 417 272 399 363 1271 290
Fragments arranged by size:
1271 417 399 363 290 272
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1959 2566 3012
Size: 1959 607 446
Fragments arranged by size:
1959 607 446
EcoO109I rG'GnC_Cy
Cuts at: 0 249 1940 2029 2268 3012
Size: 249 1691 89 239 744
Fragments arranged by size:
1691 744 249 239 89
FspI TGC'GCA
Cuts at: 0 846 3012
Size: 846 2166
HaeII r_GCGC'y
Cuts at: 0 496 729 1850 2504 3012
Size: 496 233 1121 654 508
Fragments arranged by size:
1121 654 508 496 233
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1045 1077 1249 1281 1484 1516 2221 2253
Size: 1045 32 172 32 203 32 705 32
Cuts at: 2253 2827 2859 3012
Size: 574 32 153
Fragments arranged by size:
1045 705 574 203 172 153 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 2404 3012
Size: 2404 608
KasI G'GCGC_C
Cuts at: 0 1846 3012
Size: 1846 1166
KpnI G_GTAC'C
Cuts at: 0 2660 3012
Size: 2660 352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1259 3012
Size: 1259 1753
MscI TGG'CCA
Cuts at: 0 2495 3012
Size: 2495 517
MslI CAynn'nnrTG
Cuts at: 0 2330 2458 3012
Size: 2330 128 554
Fragments arranged by size:
2330 554 128
MspA1I CmG'CkG
Cuts at: 0 38 696 1153 1240 1617 2908 3012
Size: 38 658 457 87 377 1291 104
Fragments arranged by size:
1291 658 457 377 104 87 38
NaeI GCC'GGC
Cuts at: 0 2531 3012
Size: 2531 481
NarI GG'CG_CC
Cuts at: 0 1847 3012
Size: 1847 1165
NgoMIV G'CCGG_C
Cuts at: 0 2529 3012
Size: 2529 483
NspI r_CATG'y
Cuts at: 0 66 651 1228 1575 1792 3012
Size: 66 585 577 347 217 1220
Fragments arranged by size:
1220 585 577 347 217 66
PasI CC'CwG_GG
Cuts at: 0 569 883 938 2157 3012
Size: 569 314 55 1219 855
Fragments arranged by size:
1219 855 569 314 55
PciI A'CATG_T
Cuts at: 0 647 3012
Size: 647 2365
PflMI CCAn_nnn'nTGG
Cuts at: 0 162 1474 2098 3012
Size: 162 1312 624 914
Fragments arranged by size:
1312 914 624 162
PfoI T'CCnGG_A
Cuts at: 0 233 1467 2699 3012
Size: 233 1234 1232 313
Fragments arranged by size:
1234 1232 313 233
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 352 384 3012
Size: 352 32 2628
Fragments arranged by size:
2628 352 32
PpuMI rG'GwC_Cy
Cuts at: 0 249 1940 2029 2268 3012
Size: 249 1691 89 239 744
Fragments arranged by size:
1691 744 249 239 89
PshAI GACnn'nnGTC
Cuts at: 0 2389 3012
Size: 2389 623
PstI C_TGCA'G
Cuts at: 0 1053 2540 3012
Size: 1053 1487 472
Fragments arranged by size:
1487 1053 472
PvuII CAG'CTG
Cuts at: 0 38 3012
Size: 38 2974
SacI G_AGCT'C
Cuts at: 0 1759 3012
Size: 1759 1253
SacII CC_GC'GG
Cuts at: 0 1154 3012
Size: 1154 1858
SanDI GG'GwC_CC
Cuts at: 0 249 3012
Size: 249 2763
SfcI C'TryA_G
Cuts at: 0 476 678 1049 1488 1760 2536 3012
Size: 476 202 371 439 272 776 476
Fragments arranged by size:
776 476 476 439 371 272 202
SfoI GGC'GCC
Cuts at: 0 1848 3012
Size: 1848 1164
SmaI CCC'GGG
Cuts at: 0 254 1221 1536 3012
Size: 254 967 315 1476
Fragments arranged by size:
1476 967 315 254
SnaBI TAC'GTA
Cuts at: 0 2415 3012
Size: 2415 597
SphI G_CATG'C
Cuts at: 0 1228 3012
Size: 1228 1784
SrfI GCCC'GGGC
Cuts at: 0 1221 1536 3012
Size: 1221 315 1476
Fragments arranged by size:
1476 1221 315
StuI AGG'CCT
Cuts at: 0 510 658 3012
Size: 510 148 2354
Fragments arranged by size:
2354 510 148
StyI C'CwwG_G
Cuts at: 0 333 779 953 1181 2673 3012
Size: 333 446 174 228 1492 339
Fragments arranged by size:
1492 446 339 333 228 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 748 804 1054 1515 2060 2908 2939 3012
Size: 748 56 250 461 545 848 31 73
Fragments arranged by size:
848 748 545 461 250 73 56 31
TatI w'GTAC_w
Cuts at: 0 650 3012
Size: 650 2362
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1010 1042 2112 2144 3012
Size: 1010 32 1070 32 868
Fragments arranged by size:
1070 1010 868 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 190 3012
Size: 190 2822
XmaI C'CCGG_G
Cuts at: 0 252 1219 1534 3012
Size: 252 967 315 1478
Fragments arranged by size:
1478 967 315 252
ZraI GAC'GTC
Cuts at: 0 1305 3012
Size: 1305 1707
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AleI AlwNI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmrI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspMI BsrDI BsrFI BsrGI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PasI PciI PflMI PfoI
PpiI PpuMI PshAI PstI PvuII SacI SacII SanDI
SfcI SfoI SmaI SnaBI SphI SrfI StuI StyI
TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AgeI AhdI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI BaeI BclI BfrBI BmgBI BmtI
BplI BpuEI BsaI BsaBI BsiWI BspHI BsrBI BssHII
BssSI BstAPI BstBI BstZ17I DraI EciI EcoRI EcoRV
FalI FseI FspAI HincII HpaI MfeI MluI NcoI
NdeI NheI NotI NruI NsiI PacI PmeI PmlI
PsiI PspOMI PspXI PsrI PvuI RsrII SalI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SpeI
SspI SwaI Tth111I XbaI XhoI XmnI