Restriction map for
pFN21AA1568
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII AccIII AcyI AflII AflIII
AgeI AocI Aor51HI ApaI AscI
AseI AsnI BbeI BbrPI BbvCI
BcgI BfrI BglI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrGI BssHII
BstEII BstPI Bsu36I CelII ClaI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoT22I HaeII Hin1I HpaI KasI
MamI MfeI MluI MroI MunI
NarI NheI NotI NruI NsiI
NspI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI PvuI RcaI
SalI SapI SbfI ScaI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 600 GTATAC GT^[AC][GT]AC
AclI 1145 AACGTT AA^CGTT
AhdI 2022 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 286 GTGCAC G^TGCAC
ApaLI 286 GTGCAC G^TGCAC
Asp700I 3998 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2022 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1515 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 470 CCCGGG C^[CT]CG[GA]G
AvrII 4027 CCTAGG C^CTAGG
BciVI 545 GTATCC GTATCC(6/5)
BclI 3114 TGATCA T^GATCA
BlnI 4027 CCTAGG C^CTAGG
BsmI 3009 GCATTC GAATGC(1/-1)
BsoBI 470 CCCGGG C^[CT]CG[GA]G
BsrFI 167 GCCGGC [GA]^CCGG[CT]
BssSI 2790 CACGAG CACGAG(-5/-1)
Bst1107I 600 GTATAC GTA^TAC
BstAPI 3483 GCAACCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 515 TTCGAA TT^CGAA
BstZ17I 600 GTATAC GTA^TAC
Cfr10I 167 GCCGGC [GA]^CCGG[CT]
CpoI 2389 CGGTCCG CG^G[AT]CCG
Eam1105I 2022 GACTTCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1849 GATATC GAT^ATC
FbaI 3114 TGATCA T^GATCA
FseI 166 GGCCGGCC GGCCGG^CC
HindIII 3677 AAGCTT A^AGCTT
KspI 91 CCGCGG CCGC^GG
NaeI 167 GCCGGC GCC^GGC
NcoI 2764 CCATGG C^CATGG
NgoMIV 167 GCCGGC G^CCGGC
NspV 515 TTCGAA TT^CGAA
PflFI 1515 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3383 GGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1145 AACGTT AA^CGTT
RsrII 2389 CGGTCCG CG^G[AT]CCG
SacI 932 GAGCTC GAGCT^C
SacII 91 CCGCGG CCGC^GG
SexAI 698 ACCAGGT A^CC[AT]GGT
SfuI 515 TTCGAA TT^CGAA
SmaI 470 CCCGGG CCC^GGG
SmlI 977 CTCAAG C^T[CT][GA]AG
Tth111I 1515 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3741 TCTAGA T^CTAGA
XmaCI 470 CCCGGG C^CCGGG
XmaI 470 CCCGGG C^CCGGG
XmnI 3998 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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Acc65I 613 GGTACC G^GTACC
1633 GGTACC
AlwNI 1517 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
1829 CAGATCCTG
Asp718I 613 GGTACC G^GTACC
1633 GGTACC
AviII 278 TGCGCA TGC^GCA
1838 TGCGCA
BalI 2782 TGGCCA TGG^CCA
3981 TGGCCA
BsaI 1121 GAGACC GGTCTC(1/5)
1804 GGTCTC
BspMI 1559 ACCTGC ACCTGC(4/8)
1584 GCAGGT
BstXI 617 CCAATATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3714 CCAGACTCCTGG
DraII 3383 GGGTCCC [GA]G^G[ACGT]CC[CT]
3694 AGGCCCC
EcoO109I 3383 GGGTCCC [GA]G^G[ACGT]CC[CT]
3694 AGGCCCC
FauI 90 CCCGC CCCGC(4/6)
1156 GCGGG
FspI 278 TGCGCA TGC^GCA
1838 TGCGCA
HincII 2098 GTCAAC GT[CT]^[GA]AC
3513 GTTGAC
HindII 2098 GTCAAC GT[CT]^[GA]AC
3513 GTTGAC
KpnI 613 GGTACC GGTAC^C
1633 GGTACC
MluNI 2782 TGGCCA TGG^CCA
3981 TGGCCA
MscI 2782 TGGCCA TGG^CCA
3981 TGGCCA
NdeI 2631 CATATG CA^TATG
2987 CATATG
PshAI 1813 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3437 GACAGCAGTC
StuI 3850 AGGCCT AGG^CCT
3938 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BamHI
BanI BanII BbsI BbvI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoNI EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII