(Circular) (Six-base) MAPSORT of: pFN21AA1568.seq Check: 4558 from: 1 to: 8839 pFN21AA1568 8839 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6649 278 Size: 53 83 186 936 5113 2468 Fragments arranged by size: 5113 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2606 6182 1937 Size: 669 3576 4594 Fragments arranged by size: 4594 3576 669 Acc65I G'GTAC_C Cuts at: 2618 3638 6160 2618 Size: 1020 2522 5297 Fragments arranged by size: 5297 2522 1020 AclI AA'CG_TT Cuts at: 1563 3151 6966 7339 1563 Size: 1588 3815 373 3063 Fragments arranged by size: 3815 3063 1588 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4014 4221 6902 8286 8554 1632 Size: 2382 207 2681 1384 268 1917 Fragments arranged by size: 2681 2382 1917 1384 268 207 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8642 AflIII A'CryG_T Cuts at: 1203 7738 8715 1203 Size: 6535 977 1327 Fragments arranged by size: 6535 1327 977 AgeI A'CCGG_T Cuts at: 7670 7670 Size: 8839 AhdI GACnn_n'nnGTC Cuts at: 4032 7568 4032 Size: 3536 5303 AleI CACnn'nnGTG Cuts at: 1166 4565 1166 Size: 3399 5440 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5469 1184 Size: 4285 4554 AlwNI CAG_nnn'CTG Cuts at: 3527 3839 6582 8154 3527 Size: 312 2743 1572 4212 Fragments arranged by size: 4212 2743 1572 312 ApaLI G'TGCA_C Cuts at: 2291 6896 8052 2291 Size: 4605 1156 3078 Fragments arranged by size: 4605 3078 1156 ApoI r'AATT_y Cuts at: 1493 2732 3433 4060 4843 6148 6374 6526 8635 Size: 1239 701 627 783 1305 226 152 2109 Cuts at: 8635 1493 Size: 1697 Fragments arranged by size: 2109 1697 1305 1239 783 701 627 226 152 AseI AT'TA_AT Cuts at: 160 1032 7393 160 Size: 872 6361 1606 Fragments arranged by size: 6361 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8839 AvaI C'yCGr_G Cuts at: 1870 1943 2475 6164 1870 Size: 73 532 3689 4545 Fragments arranged by size: 4545 3689 532 73 AvrII C'CTAG_G Cuts at: 6032 6032 Size: 8839 BamHI G'GATC_C Cuts at: 2267 4285 5201 5729 6020 6169 6536 2267 Size: 2018 916 528 291 149 367 4570 Fragments arranged by size: 4570 2018 916 528 367 291 149 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2618 2870 3638 4604 5316 Size: 325 217 627 831 252 768 966 712 Cuts at: 5316 5950 6160 7615 618 Size: 634 210 1455 1842 Fragments arranged by size: 1842 1455 966 831 768 712 634 627 325 252 217 210 BanII G_rGCy'C Cuts at: 1391 2145 2941 6107 6158 1391 Size: 754 796 3166 51 4072 Fragments arranged by size: 4072 3166 796 754 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2066 3917 4733 928 Size: 971 167 1851 816 5034 Fragments arranged by size: 5034 1851 971 816 167 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7030 7064 1903 Size: 34 5093 34 3678 Fragments arranged by size: 5093 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2561 6731 7941 2561 Size: 4170 1210 3459 Fragments arranged by size: 4170 3459 1210 BclI T'GATC_A Cuts at: 1548 5119 1548 Size: 3571 5268 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7450 136 Size: 107 122 71 631 605 5778 1525 Fragments arranged by size: 5778 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4332 5007 5089 8834 4332 Size: 675 82 3745 4337 Fragments arranged by size: 4337 3745 675 82 BlpI GC'TnA_GC Cuts at: 6256 6256 Size: 8839 Bme1580I G_kGCm'C Cuts at: 1790 2295 4219 5321 5955 6900 8056 1790 Size: 505 1924 1102 634 945 1156 2573 Fragments arranged by size: 2573 1924 1156 1102 945 634 505 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8839 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3985 5231 5961 Size: 416 53 83 399 24 2568 1246 730 Cuts at: 5961 6570 7528 442 Size: 609 958 1753 Fragments arranged by size: 2568 1753 1246 958 730 609 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8839 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2463 2480 2735 3144 3242 3520 3549 4281 Size: 1224 17 255 409 98 278 29 732 Cuts at: 4281 5577 5640 5964 7499 1239 Size: 1296 63 324 1535 2579 Fragments arranged by size: 2579 1535 1296 1224 732 409 324 278 255 98 63 29 17 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2967 6964 7829 8127 2967 Size: 3997 865 298 3679 Fragments arranged by size: 3997 3679 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3120 3815 7502 882 Size: 617 1621 695 3687 2219 Fragments arranged by size: 3687 2219 1621 695 617 BsaAI yAC'GTr Cuts at: 493 1204 6630 493 Size: 711 5426 2702 Fragments arranged by size: 5426 2702 711 BsaBI GATnn'nnATC Cuts at: 8833 8833 Size: 8839 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6646 7028 7663 275 Size: 53 83 186 936 5113 382 635 1451 Fragments arranged by size: 5113 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7272 7670 7944 8091 8502 7272 Size: 398 274 147 411 7609 Fragments arranged by size: 7609 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5449 5479 1178 Size: 30 165 30 4046 30 4538 Fragments arranged by size: 4538 4046 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4281 4284 4716 5770 5954 1186 Size: 3095 3 432 1054 184 4071 Fragments arranged by size: 4071 3095 1054 432 184 3 BseYI C'CCAG_C Cuts at: 2261 2650 3087 3274 4611 4760 8042 8538 2261 Size: 389 437 187 1337 149 3282 496 2562 Fragments arranged by size: 3282 2562 1337 496 437 389 187 149 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3525 3914 4482 5853 1163 Size: 573 1789 389 568 1371 4149 Fragments arranged by size: 4149 1789 1371 573 568 389 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6289 7050 8078 1540 Size: 275 186 4288 761 1028 2301 Fragments arranged by size: 4288 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2295 2941 5713 6900 6985 8056 2295 Size: 646 2772 1187 85 1071 3078 Fragments arranged by size: 3078 2772 1187 1071 646 85 BsmI GAATG_Cn' Cuts at: 5014 6350 6443 5014 Size: 1336 93 7410 Fragments arranged by size: 7410 1336 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2134 2382 4501 2134 Size: 248 2119 6472 Fragments arranged by size: 6472 2119 248 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2145 2295 2941 4219 5321 5713 5955 Size: 399 355 150 646 1278 1102 392 242 Cuts at: 5955 6107 6158 6900 6985 8056 1391 Size: 152 51 742 85 1071 2174 Fragments arranged by size: 2174 1278 1102 1071 742 646 399 392 355 242 152 150 85 51 BspEI T'CCGG_A Cuts at: 8502 8502 Size: 8839 BspHI T'CATG_A Cuts at: 6728 8458 6728 Size: 1730 7109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3573 3580 6194 844 Size: 2729 7 2614 3489 Fragments arranged by size: 3489 2729 2614 7 BsrBI CCG'CTC Cuts at: 1394 1823 6726 1394 Size: 429 4903 3507 Fragments arranged by size: 4903 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4519 4729 5324 5906 6076 7334 7508 Size: 1183 3271 210 595 582 170 1258 174 Cuts at: 7508 65 Size: 1396 Fragments arranged by size: 3271 1396 1258 1183 595 582 210 174 170 BsrFI r'CCGG_y Cuts at: 1700 1810 2172 7483 7670 1700 Size: 110 362 5311 187 2869 Fragments arranged by size: 5311 2869 362 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8839 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8839 BssSI C'ACGA_G Cuts at: 4795 6899 7911 4795 Size: 2104 1012 5723 Fragments arranged by size: 5723 2104 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5494 794 Size: 4700 4139 BstBI TT'CG_AA Cuts at: 2521 7643 2521 Size: 5122 3717 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8839 BstXI CCAn_nnnn'nTGG Cuts at: 2629 5726 2629 Size: 3097 5742 BstYI r'GATC_y Cuts at: 1972 2267 2755 3388 3835 4285 4332 5007 5089 Size: 295 488 633 447 450 47 675 82 Cuts at: 5089 5201 5729 6020 6169 6536 6921 6938 8390 Size: 112 528 291 149 367 385 17 1452 Cuts at: 8390 8476 8488 8834 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 675 633 528 488 450 447 385 367 346 295 291 149 112 86 82 47 17 12 BstZ17I GTA'TAC Cuts at: 2607 2607 Size: 8839 BtgI C'CryG_G Cuts at: 513 1065 2096 2554 3714 3954 4769 4852 5474 Size: 552 1031 458 1160 240 815 83 622 Cuts at: 5474 513 Size: 3878 Fragments arranged by size: 3878 1160 1031 815 622 552 458 240 83 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8100 Fragments arranged by size: 8100 683 56 BtsI GCAGTG_nn' Cuts at: 2367 3300 3419 3513 5283 5451 6355 7149 7169 Size: 933 119 94 1770 168 904 794 20 Cuts at: 7169 2367 Size: 4037 Fragments arranged by size: 4037 1770 933 904 794 168 119 94 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8839 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2410 2445 529 Size: 35 1846 35 6923 Fragments arranged by size: 6923 1846 35 35 DraI TTT'AAA Cuts at: 6143 6143 Size: 8839 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7846 809 Size: 507 6530 1802 Fragments arranged by size: 6530 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2174 2960 4142 Size: 54 1425 50 275 18 344 786 1182 Cuts at: 4142 4787 4849 5986 6286 7175 8804 8 Size: 645 62 1137 300 889 1629 43 Fragments arranged by size: 1629 1425 1182 1137 889 786 645 344 300 275 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6286 1537 Size: 275 4474 4090 Fragments arranged by size: 4474 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3345 4655 5182 5475 5670 6769 1353 Size: 1992 1310 527 293 195 1099 3423 Fragments arranged by size: 3423 1992 1310 1099 527 293 195 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2180 5902 7426 7800 7946 387 Size: 1064 138 591 3722 1524 374 146 1280 Fragments arranged by size: 3722 1524 1280 1064 591 374 146 138 EcoICRI GAG'CTC Cuts at: 2939 2939 Size: 8839 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2463 2480 2735 3144 3242 3520 3549 Size: 393 831 17 255 409 98 278 29 Cuts at: 3549 4014 4221 4281 5577 5640 5964 6902 7499 Size: 465 207 60 1296 63 324 938 597 Cuts at: 7499 8286 8554 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1296 938 831 787 597 465 409 393 324 278 268 255 207 98 63 60 29 17 EcoNI CCTnn'n_nnAGG Cuts at: 2316 4112 5406 2316 Size: 1796 1294 5749 Fragments arranged by size: 5749 1796 1294 EcoO109I rG'GnC_Cy Cuts at: 5389 5700 5389 Size: 311 8528 EcoRI G'AATT_C Cuts at: 6148 6526 6148 Size: 378 8461 EcoRV GAT'ATC Cuts at: 3856 3856 Size: 8839 FseI GG_CCGG'CC Cuts at: 2176 2176 Size: 8839 FspI TGC'GCA Cuts at: 2285 3845 7345 2285 Size: 1560 3500 3779 Fragments arranged by size: 3779 3500 1560 FspAI rTGC'GCAy Cuts at: 3845 3845 Size: 8839 HaeII r_GCGC'y Cuts at: 1678 7986 1678 Size: 6308 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2225 2257 2332 2364 2965 Size: 32 135 32 653 32 75 32 601 Cuts at: 2965 2997 3522 3554 3964 3996 4006 4018 4038 Size: 32 525 32 410 32 10 12 20 Cuts at: 4038 4050 4163 4195 4324 4350 4356 4382 5833 Size: 12 113 32 129 26 6 26 1451 Cuts at: 5833 5865 7480 7512 7554 7586 1373 Size: 32 1615 32 42 32 2626 Fragments arranged by size: 2626 1615 1451 653 601 525 410 135 129 113 75 42 32 32 32 32 32 32 32 32 32 32 32 26 26 20 12 12 10 6 HincII GTy'rAC Cuts at: 669 1657 1938 4105 5520 6183 6429 669 Size: 988 281 2167 1415 663 246 3079 Fragments arranged by size: 3079 2167 1415 988 663 281 246 HindIII A'AGCT_T Cuts at: 748 5682 748 Size: 4934 3905 HpaI GTT'AAC Cuts at: 6429 6429 Size: 8839 KpnI G_GTAC'C Cuts at: 2622 3642 6164 2622 Size: 1020 2522 5297 Fragments arranged by size: 5297 2522 1020 MfeI C'AATT_G Cuts at: 6438 6438 Size: 8839 MluI A'CGCG_T Cuts at: 8715 8715 Size: 8839 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2434 2881 3180 4228 4450 5043 6132 7953 Size: 1129 447 299 1048 222 593 1089 1821 Cuts at: 7953 8137 1305 Size: 184 2007 Fragments arranged by size: 2007 1821 1129 1089 1048 593 447 299 222 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4789 5988 10 Size: 54 1425 343 2957 1199 2861 Fragments arranged by size: 2957 2861 1425 1199 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2876 4051 4308 4565 5511 6797 7156 Size: 648 1710 1175 257 257 946 1286 359 Cuts at: 7156 7315 518 Size: 159 2042 Fragments arranged by size: 2042 1710 1286 1175 946 648 359 257 257 159 MspA1I CmG'CkG Cuts at: 1606 2098 2265 2441 3035 3705 6255 6932 8080 Size: 492 167 176 594 670 2550 677 1148 Cuts at: 8080 8325 1606 Size: 245 2120 Fragments arranged by size: 2550 2120 1148 677 670 594 492 245 176 167 NaeI GCC'GGC Cuts at: 2174 2174 Size: 8839 NcoI C'CATG_G Cuts at: 513 1065 4769 513 Size: 552 3704 4583 Fragments arranged by size: 4583 3704 552 NdeI CA'TA_TG Cuts at: 387 4637 4993 387 Size: 4250 356 4233 Fragments arranged by size: 4250 4233 356 NgoMIV G'CCGG_C Cuts at: 2172 2172 Size: 8839 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8839 NotI GC'GGCC_GC Cuts at: 6286 6286 Size: 8839 NspI r_CATG'y Cuts at: 1132 1229 6197 7742 1132 Size: 97 4968 1545 2229 Fragments arranged by size: 4968 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8839 PciI A'CATG_T Cuts at: 7738 7738 Size: 8839 PflMI CCAn_nnn'nTGG Cuts at: 1108 2193 2502 3289 4168 5356 1108 Size: 1085 309 787 879 1188 4591 Fragments arranged by size: 4591 1188 1085 879 787 309 PfoI T'CCnGG_A Cuts at: 2457 3497 5725 2457 Size: 1040 2228 5571 Fragments arranged by size: 5571 2228 1040 PmeI GTTT'AAAC Cuts at: 6143 6143 Size: 8839 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6908 6940 8421 8453 6908 Size: 32 1481 32 7294 Fragments arranged by size: 7294 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 5389 5389 Size: 8839 PshAI GACnn'nnGTC Cuts at: 1613 3822 5446 1613 Size: 2209 1624 5006 Fragments arranged by size: 5006 2209 1624 PsiI TTA'TAA Cuts at: 6409 6409 Size: 8839 PstI C_TGCA'G Cuts at: 830 3426 4041 5502 6191 830 Size: 2596 615 1461 689 3478 Fragments arranged by size: 3478 2596 1461 689 615 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8839 PvuII CAG'CTG Cuts at: 2441 3035 3705 2441 Size: 594 670 7575 Fragments arranged by size: 7575 670 594 RsrII CG'GwC_CG Cuts at: 4395 4395 Size: 8839 SacI G_AGCT'C Cuts at: 2941 2941 Size: 8839 SacII CC_GC'GG Cuts at: 2099 2099 Size: 8839 SalI G'TCGA_C Cuts at: 1936 6181 1936 Size: 4245 4594 SanDI GG'GwC_CC Cuts at: 5389 5389 Size: 8839 SbfI CC_TGCA'GG Cuts at: 6191 6191 Size: 8839 ScaI AGT'ACT Cuts at: 7087 8799 7087 Size: 1712 7127 SexAI A'CCwGG_T Cuts at: 2703 2703 Size: 8839 SfcI C'TryA_G Cuts at: 826 1045 2727 3422 4037 5498 5956 6187 7322 Size: 219 1682 695 615 1461 458 231 1135 Cuts at: 7322 8003 8194 826 Size: 681 191 1471 Fragments arranged by size: 1682 1471 1461 1135 695 681 615 458 231 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8839 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8839 SmaI CCC'GGG Cuts at: 1872 2477 6166 1872 Size: 605 3689 4545 Fragments arranged by size: 4545 3689 605 SmlI C'TyrA_G Cuts at: 820 1017 1943 2982 6943 7844 8106 820 Size: 197 926 1039 3961 901 262 1553 Fragments arranged by size: 3961 1553 1039 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6630 493 Size: 6137 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8839 SphI G_CATG'C Cuts at: 1132 6197 1132 Size: 5065 3774 SspI AAT'ATT Cuts at: 5 52 6763 5 Size: 47 6711 2081 Fragments arranged by size: 6711 2081 47 StuI AGG'CCT Cuts at: 1517 5857 5945 1517 Size: 4340 88 4411 Fragments arranged by size: 4411 4340 88 StyI C'CwwG_G Cuts at: 513 1065 2749 3805 4000 4162 4769 4882 5350 Size: 552 1684 1056 195 162 607 113 468 Cuts at: 5350 5946 6032 6278 513 Size: 596 86 246 3074 Fragments arranged by size: 3074 1684 1056 607 596 552 468 246 195 162 113 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2499 4383 6866 6883 7036 7221 7666 Size: 1255 919 1884 2483 17 153 185 445 Cuts at: 7666 325 Size: 1498 Fragments arranged by size: 2483 1884 1498 1255 919 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2858 4237 5332 Size: 274 80 33 51 1443 881 1379 1095 Cuts at: 5332 7085 8797 96 Size: 1753 1712 138 Fragments arranged by size: 1753 1712 1443 1379 1095 881 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2916 2948 3319 3351 8799 8831 2916 Size: 32 371 32 5448 32 2924 Fragments arranged by size: 5448 2924 371 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3523 1316 Size: 2207 6632 XbaI T'CTAG_A Cuts at: 5746 6175 5746 Size: 429 8410 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2772 3039 3286 5249 2772 Size: 267 247 1963 6362 Fragments arranged by size: 6362 1963 267 247 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8839 XmaI C'CCGG_G Cuts at: 1870 2475 6164 1870 Size: 605 3689 4545 Fragments arranged by size: 4545 3689 605 XmnI GAAnn'nnTTC Cuts at: 6007 6968 6007 Size: 961 7878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6647 276 Size: 53 83 186 936 5113 2468 Fragments arranged by size: 5113 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI Bsu36I DraIII FalI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI SapI SfiI SfoI SrfI SwaI