Restriction map for
pFN21AA1568
(Linear) (Six-base) MAPSORT of: KIAA1568.seq Check: 5298 from: 1 to: 4134
KIAA1568.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 601 4134
Size: 601 3533
Acc65I G'GTAC_C
Cuts at: 0 613 1633 4134
Size: 613 1020 2501
Fragments arranged by size:
2501 1020 613
AclI AA'CG_TT
Cuts at: 0 1146 4134
Size: 1146 2988
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2009 2216 4134
Size: 2009 207 1918
Fragments arranged by size:
2009 1918 207
AhdI GACnn_n'nnGTC
Cuts at: 0 2027 4134
Size: 2027 2107
AleI CACnn'nnGTG
Cuts at: 0 2560 4134
Size: 2560 1574
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3464 4134
Size: 3464 670
AlwNI CAG_nnn'CTG
Cuts at: 0 1522 1834 4134
Size: 1522 312 2300
Fragments arranged by size:
2300 1522 312
ApaLI G'TGCA_C
Cuts at: 0 286 4134
Size: 286 3848
ApoI r'AATT_y
Cuts at: 0 727 1428 2055 2838 4134
Size: 727 701 627 783 1296
Fragments arranged by size:
1296 783 727 701 627
AvaI C'yCGr_G
Cuts at: 0 470 4134
Size: 470 3664
AvrII C'CTAG_G
Cuts at: 0 4027 4134
Size: 4027 107
BamHI G'GATC_C
Cuts at: 0 262 2280 3196 3724 4015 4134
Size: 262 2018 916 528 291 119
Fragments arranged by size:
2018 916 528 291 262 119
BanI G'GyrC_C
Cuts at: 0 613 865 1633 2599 3311 3945 4134
Size: 613 252 768 966 712 634 189
Fragments arranged by size:
966 768 712 634 613 252 189
BanII G_rGCy'C
Cuts at: 0 140 936 4102 4134
Size: 140 796 3166 32
Fragments arranged by size:
3166 796 140 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 61 1912 2728 4134
Size: 61 1851 816 1406
Fragments arranged by size:
1851 1406 816 61
BciVI GTATCCnnnnn_n'
Cuts at: 0 556 4134
Size: 556 3578
BclI T'GATC_A
Cuts at: 0 3114 4134
Size: 3114 1020
BglII A'GATC_T
Cuts at: 0 2327 3002 3084 4134
Size: 2327 675 82 1050
Fragments arranged by size:
2327 1050 675 82
Bme1580I G_kGCm'C
Cuts at: 0 290 2214 3316 3950 4134
Size: 290 1924 1102 634 184
Fragments arranged by size:
1924 1102 634 290 184
BmrI ACTGGGnnnn_n'
Cuts at: 0 1980 3226 3956 4134
Size: 1980 1246 730 178
Fragments arranged by size:
1980 1246 730 178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 475 730 1139 1237 1515 1544 2276
Size: 458 17 255 409 98 278 29 732
Cuts at: 2276 3572 3635 3959 4134
Size: 1296 63 324 175
Fragments arranged by size:
1296 732 458 409 324 278 255 175
98 63 29 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 4134
Size: 962 3172
BsaI GGTCTCn'nnnn_
Cuts at: 0 1115 1810 4134
Size: 1115 695 2324
Fragments arranged by size:
2324 1115 695
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3444 3474 4134
Size: 3444 30 660
Fragments arranged by size:
3444 660 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2276 2279 2711 3765 3949 4134
Size: 2276 3 432 1054 184 185
Fragments arranged by size:
2276 1054 432 185 184 3
BseYI C'CCAG_C
Cuts at: 0 256 645 1082 1269 2606 2755 4134
Size: 256 389 437 187 1337 149 1379
Fragments arranged by size:
1379 1337 437 389 256 187 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1520 1909 2477 3848 4134
Size: 1520 389 568 1371 286
Fragments arranged by size:
1520 1371 568 389 286
BsiHKAI G_wGCw'C
Cuts at: 0 290 936 3708 4134
Size: 290 646 2772 426
Fragments arranged by size:
2772 646 426 290
BsmI GAATG_Cn'
Cuts at: 0 3009 4134
Size: 3009 1125
BsmBI CGTCTCn'nnnn_
Cuts at: 0 129 377 2496 4134
Size: 129 248 2119 1638
Fragments arranged by size:
2119 1638 248 129
Bsp1286I G_dGCh'C
Cuts at: 0 140 290 936 2214 3316 3708 3950 4102
Size: 140 150 646 1278 1102 392 242 152
Cuts at: 4102 4134
Size: 32
Fragments arranged by size:
1278 1102 646 392 242 152 150 140
32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1568 1575 4134
Size: 1568 7 2559
Fragments arranged by size:
2559 1568 7
BsrDI GCAATG_nn'
Cuts at: 0 2514 2724 3319 3901 4071 4134
Size: 2514 210 595 582 170 63
Fragments arranged by size:
2514 595 582 210 170 63
BsrFI r'CCGG_y
Cuts at: 0 167 4134
Size: 167 3967
BssSI C'ACGA_G
Cuts at: 0 2790 4134
Size: 2790 1344
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3489 4134
Size: 3489 645
BstBI TT'CG_AA
Cuts at: 0 516 4134
Size: 516 3618
BstXI CCAn_nnnn'nTGG
Cuts at: 0 624 3721 4134
Size: 624 3097 413
Fragments arranged by size:
3097 624 413
BstYI r'GATC_y
Cuts at: 0 262 750 1383 1830 2280 2327 3002 3084
Size: 262 488 633 447 450 47 675 82
Cuts at: 3084 3196 3724 4015 4134
Size: 112 528 291 119
Fragments arranged by size:
675 633 528 488 450 447 291 262
119 112 82 47
BstZ17I GTA'TAC
Cuts at: 0 602 4134
Size: 602 3532
BtgI C'CryG_G
Cuts at: 0 91 549 1709 1949 2764 2847 3469 4134
Size: 91 458 1160 240 815 83 622 665
Fragments arranged by size:
1160 815 665 622 458 240 91 83
BtsI GCAGTG_nn'
Cuts at: 0 362 1295 1414 1508 3278 3446 4134
Size: 362 933 119 94 1770 168 688
Fragments arranged by size:
1770 933 688 362 168 119 94
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 405 440 4134
Size: 405 35 3694
Fragments arranged by size:
3694 405 35
EaeI y'GGCC_r
Cuts at: 0 169 955 2137 2782 2844 3981 4134
Size: 169 786 1182 645 62 1137 153
Fragments arranged by size:
1182 1137 786 645 169 153 62
EarI CTCTTCn'nnn_
Cuts at: 0 1340 2650 3177 3470 3665 4134
Size: 1340 1310 527 293 195 469
Fragments arranged by size:
1340 1310 527 469 293 195
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 175 3897 4134
Size: 175 3722 237
Fragments arranged by size:
3722 237 175
EcoICRI GAG'CTC
Cuts at: 0 934 4134
Size: 934 3200
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 475 730 1139 1237 1515 1544 2009
Size: 458 17 255 409 98 278 29 465
Cuts at: 2009 2216 2276 3572 3635 3959 4134
Size: 207 60 1296 63 324 175
Fragments arranged by size:
1296 465 458 409 324 278 255 207
175 98 63 60 29 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 311 2107 3401 4134
Size: 311 1796 1294 733
Fragments arranged by size:
1796 1294 733 311
EcoO109I rG'GnC_Cy
Cuts at: 0 3384 3695 4134
Size: 3384 311 439
Fragments arranged by size:
3384 439 311
EcoRV GAT'ATC
Cuts at: 0 1851 4134
Size: 1851 2283
FseI GG_CCGG'CC
Cuts at: 0 171 4134
Size: 171 3963
FspI TGC'GCA
Cuts at: 0 280 1840 4134
Size: 280 1560 2294
Fragments arranged by size:
2294 1560 280
FspAI rTGC'GCAy
Cuts at: 0 1840 4134
Size: 1840 2294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 327 359 960 992 1517 1549
Size: 220 32 75 32 601 32 525 32
Cuts at: 1549 1959 1991 2001 2013 2033 2045 2158 2190
Size: 410 32 10 12 20 12 113 32
Cuts at: 2190 2319 2345 2351 2377 3828 3860 4134
Size: 129 26 6 26 1451 32 274
Fragments arranged by size:
1451 601 525 410 274 220 129 113
75 32 32 32 32 32 32 32
26 26 20 12 12 10 6
HincII GTy'rAC
Cuts at: 0 2100 3515 4134
Size: 2100 1415 619
Fragments arranged by size:
2100 1415 619
HindIII A'AGCT_T
Cuts at: 0 3677 4134
Size: 3677 457
KpnI G_GTAC'C
Cuts at: 0 617 1637 4134
Size: 617 1020 2497
Fragments arranged by size:
2497 1020 617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 429 876 1175 2223 2445 3038 4127 4134
Size: 429 447 299 1048 222 593 1089 7
Fragments arranged by size:
1089 1048 593 447 429 299 222 7
MscI TGG'CCA
Cuts at: 0 2784 3983 4134
Size: 2784 1199 151
Fragments arranged by size:
2784 1199 151
MslI CAynn'nnrTG
Cuts at: 0 871 2046 2303 2560 3506 4134
Size: 871 1175 257 257 946 628
Fragments arranged by size:
1175 946 871 628 257 257
MspA1I CmG'CkG
Cuts at: 0 93 260 436 1030 1700 4134
Size: 93 167 176 594 670 2434
Fragments arranged by size:
2434 670 594 176 167 93
NaeI GCC'GGC
Cuts at: 0 169 4134
Size: 169 3965
NcoI C'CATG_G
Cuts at: 0 2764 4134
Size: 2764 1370
NdeI CA'TA_TG
Cuts at: 0 2632 2988 4134
Size: 2632 356 1146
Fragments arranged by size:
2632 1146 356
NgoMIV G'CCGG_C
Cuts at: 0 167 4134
Size: 167 3967
PflMI CCAn_nnn'nTGG
Cuts at: 0 188 497 1284 2163 3351 4134
Size: 188 309 787 879 1188 783
Fragments arranged by size:
1188 879 787 783 309 188
PfoI T'CCnGG_A
Cuts at: 0 452 1492 3720 4134
Size: 452 1040 2228 414
Fragments arranged by size:
2228 1040 452 414
PpuMI rG'GwC_Cy
Cuts at: 0 3384 4134
Size: 3384 750
PshAI GACnn'nnGTC
Cuts at: 0 1817 3441 4134
Size: 1817 1624 693
Fragments arranged by size:
1817 1624 693
PstI C_TGCA'G
Cuts at: 0 1421 2036 3497 4134
Size: 1421 615 1461 637
Fragments arranged by size:
1461 1421 637 615
PvuII CAG'CTG
Cuts at: 0 436 1030 1700 4134
Size: 436 594 670 2434
Fragments arranged by size:
2434 670 594 436
RsrII CG'GwC_CG
Cuts at: 0 2390 4134
Size: 2390 1744
SacI G_AGCT'C
Cuts at: 0 936 4134
Size: 936 3198
SacII CC_GC'GG
Cuts at: 0 94 4134
Size: 94 4040
SanDI GG'GwC_CC
Cuts at: 0 3384 4134
Size: 3384 750
SexAI A'CCwGG_T
Cuts at: 0 698 4134
Size: 698 3436
SfcI C'TryA_G
Cuts at: 0 722 1417 2032 3493 3951 4134
Size: 722 695 615 1461 458 183
Fragments arranged by size:
1461 722 695 615 458 183
SmaI CCC'GGG
Cuts at: 0 472 4134
Size: 472 3662
SmlI C'TyrA_G
Cuts at: 0 977 4134
Size: 977 3157
StuI AGG'CCT
Cuts at: 0 3852 3940 4134
Size: 3852 88 194
Fragments arranged by size:
3852 194 88
StyI C'CwwG_G
Cuts at: 0 744 1800 1995 2157 2764 2877 3345 3941
Size: 744 1056 195 162 607 113 468 596
Cuts at: 3941 4027 4134
Size: 86 107
Fragments arranged by size:
1056 744 607 596 468 195 162 113
107 86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 494 2378 4134
Size: 494 1884 1756
Fragments arranged by size:
1884 1756 494
TatI w'GTAC_w
Cuts at: 0 853 2232 3327 4134
Size: 853 1379 1095 807
Fragments arranged by size:
1379 1095 853 807
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 911 943 1314 1346 4134
Size: 911 32 371 32 2788
Fragments arranged by size:
2788 911 371 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1518 4134
Size: 1518 2616
XbaI T'CTAG_A
Cuts at: 0 3741 4134
Size: 3741 393
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 767 1034 1281 3244 4134
Size: 767 267 247 1963 890
Fragments arranged by size:
1963 890 767 267 247
XmaI C'CCGG_G
Cuts at: 0 470 4134
Size: 470 3664
XmnI GAAnn'nnTTC
Cuts at: 0 4002 4134
Size: 4002 132
Enzymes that do cut:
AccI Acc65I AclI AcuI AhdI AleI AlfI AlwNI
ApaLI ApoI AvaI AvrII BamHI BanI BanII BbsI
BciVI BclI BglII Bme1580I BmrI BpmI BpuEI BsaI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtsI CspCI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRV FseI FspI FspAI Hin4I
HincII HindIII KpnI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV PflMI PfoI PpuMI PshAI PstI
PvuII RsrII SacI SacII SanDI SexAI SfcI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AflIII AgeI AloI ApaI
AscI AseI AsiSI BaeI BbeI BbvCI BcgI BfrBI
BglI BlpI BmgBI BmtI BplI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrGI
BssHII BstEII Bsu36I BtgZI ClaI DraI DraIII DrdI
EagI EcoRI FalI HaeII HpaI KasI MfeI MluI
NarI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI SalI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI XhoI
ZraI