Restriction map for
pFN21AA1522
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AclI AcsI AflII AflIII
AhdI Aor51HI ApoI AscI AseI
AsnI Asp700I AspEI BamHI BbrPI
BcgI BclI BfrI BglII BsaBI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI DraI
DraIII Eam1105I Eco47III EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KspI
MamI MfeI MluI MseI MunI
NdeI NotI NruI NsiI NspV
PacI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI SacII SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 751 GTCTAC GT^[AC][GT]AC
AccII 405 CGCG CG^CG
AccIII 2864 TCCGGA T^CCGGA
AgeI 2433 ACCGGT A^CCGGT
Alw44I 602 GTGCAC G^TGCAC
ApaLI 602 GTGCAC G^TGCAC
AviII 977 TGCGCA TGC^GCA
AvrII 960 CCTAGG C^CTAGG
BbeI 1559 GGCGCC GGCGC^C
BlnI 960 CCTAGG C^CTAGG
BsaAI 376 TACGTG [CT]AC^GT[GA]
BsaI 2824 GAGACC GGTCTC(1/5)
BseAI 2864 TCCGGA T^CCGGA
BsiWI 615 CGTACG C^GTACG
BsmI 1286 GCATTC GAATGC(1/-1)
BspEI 2864 TCCGGA T^CCGGA
BspMI 1651 GCAGGT ACCTGC(4/8)
BstUI 405 CGCG CG^CG
BstXI 1640 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2151 AGATCC [GA]^GATC[CT]
DrdI 1822 GACAGGTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 977 TGCGCA TGC^GCA
KasI 1559 GGCGCC G^GCGCC
MflI 2151 AGATCC [GA]^GATC[CT]
MroI 2864 TCCGGA T^CCGGA
MvnI 405 CGCG CG^CG
NaeI 84 GCCGGC GCC^GGC
NarI 1559 GGCGCC GG^CGCC
NgoMIV 84 GCCGGC G^CCGGC
NheI 1195 GCTAGC G^CTAGC
PflMI 1520 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2433 ACCGGT A^CCGGT
PshAI 1957 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 211 GAAGAGC GCTCTTC(1/4)
SfoI 1559 GGCGCC GGC^GCC
Van91I 1520 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 2151 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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AlwI 429 GATCC GGATC(4/5)
2152 GATCC
AspI 172 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
745 GACCGTGTC
BbsI 340 GAAGAC GAAGAC(2/6)
178 GTCTTC
BciVI 1603 GGATAC GTATCC(6/5)
311 GTATCC
BpuAI 340 GAAGAC GAAGAC(2/6)
178 GTCTTC
BsaWI 2433 ACCGGT [AT]^CCGG[AT]
2864 TCCGGA
CpoI 2654 CGGTCCG CG^G[AT]CCG
2715 CGGACCG
DpnII 429 GATC ^GATC
2152 GATC
EagI 82 CGGCCG C^GGCCG
636 CGGCCG
EclXI 82 CGGCCG C^GGCCG
636 CGGCCG
Eco52I 82 CGGCCG C^GGCCG
636 CGGCCG
MboI 429 GATC ^GATC
2152 GATC
MslI 118 CATCTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1125 CACCATTGTG
NcoI 294 CCATGG C^CATGG
2204 CCATGG
NdeII 429 GATC ^GATC
2152 GATC
PaeR7I 1608 CTCGAG C^TCGAG
2981 CTCGAG
PflFI 172 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
745 GACCGTGTC
PpuMI 1653 AGGTCCC [GA]G^G[AT]CC[CT]
2195 AGGACCT
RsrII 2654 CGGTCCG CG^G[AT]CCG
2715 CGGACCG
SacI 387 GAGCTC GAGCT^C
1185 GAGCTC
Sau3AI 429 GATC ^GATC
2152 GATC
SbfI 678 CCTGCAGG CCTGCA^GG
2260 CCTGCAGG
Sse8387I 678 CCTGCAGG CCTGCA^GG
2260 CCTGCAGG
Tth111I 172 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
745 GACCGTGTC
XhoI 1608 CTCGAG C^TCGAG
2981 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
Acc65I AciI AcyI AfaI AluI
AlwNI AocI ApaI Asp718I AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboII MluNI
MnlI MscI MspA1I MspI MvaI
MwoI NciI NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I XcmI XmaCI
XmaI