(Circular) (Six-base) MAPSORT of: pFN21AA1522.seq Check: 7531 from: 1 to: 7843 pFN21AA1522 7843 bp With 182 enzymes: SgfI * October 20, 2009 10:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5653 278 Size: 53 83 186 936 4117 2468 Fragments arranged by size: 4117 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2757 5186 1937 Size: 820 2429 4594 Fragments arranged by size: 4594 2429 820 Acc65I G'GTAC_C Cuts at: 2792 3214 3535 5164 2792 Size: 422 321 1629 5471 Fragments arranged by size: 5471 1629 422 321 AclI AA'CG_TT Cuts at: 1563 5970 6343 1563 Size: 4407 373 3063 Fragments arranged by size: 4407 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3362 4260 5906 7290 7558 1632 Size: 1730 898 1646 1384 268 1917 Fragments arranged by size: 1917 1730 1646 1384 898 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7646 AflIII A'CryG_T Cuts at: 1203 6742 7719 1203 Size: 5539 977 1327 Fragments arranged by size: 5539 1327 977 AgeI A'CCGG_T Cuts at: 4438 6674 4438 Size: 2236 5607 AhdI GACnn_n'nnGTC Cuts at: 6572 6572 Size: 7843 AleI CACnn'nnGTG Cuts at: 1166 3134 1166 Size: 1968 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7843 AlwNI CAG_nnn'CTG Cuts at: 2061 2099 2156 2278 2692 2837 2861 3404 4136 Size: 38 57 122 414 145 24 543 732 Cuts at: 4136 4204 4328 4391 4631 5586 7158 2061 Size: 68 124 63 240 955 1572 2746 Fragments arranged by size: 2746 1572 955 732 543 414 240 145 124 122 68 63 57 38 24 ApaI G_GGCC'C Cuts at: 2674 2803 4606 5093 2674 Size: 129 1803 487 5424 Fragments arranged by size: 5424 1803 487 129 ApaLI G'TGCA_C Cuts at: 2607 5900 7056 2607 Size: 3293 1156 3394 Fragments arranged by size: 3394 3293 1156 ApoI r'AATT_y Cuts at: 1493 5152 5378 5530 7639 1493 Size: 3659 226 152 2109 1697 Fragments arranged by size: 3659 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6397 160 Size: 872 5365 1606 Fragments arranged by size: 5365 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7843 AvaI C'yCGr_G Cuts at: 1870 1943 2046 2595 2649 2673 2796 3613 4348 Size: 73 103 549 54 24 123 817 735 Cuts at: 4348 4986 5113 5168 1870 Size: 638 127 55 4545 Fragments arranged by size: 4545 817 735 638 549 127 123 103 73 55 54 24 AvrII C'CTAG_G Cuts at: 2965 2965 Size: 7843 BamHI G'GATC_C Cuts at: 5173 5540 5173 Size: 367 7476 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2581 2654 2792 3214 3535 Size: 325 217 627 794 73 138 422 321 Cuts at: 3535 3564 3856 4117 5164 6619 618 Size: 29 292 261 1047 1455 1842 Fragments arranged by size: 1842 1455 1047 794 627 422 325 321 292 261 217 138 73 29 BanII G_rGCy'C Cuts at: 1391 2247 2396 2602 2674 2803 2871 2879 3194 Size: 856 149 206 72 129 68 8 315 Cuts at: 3194 3248 3899 4149 4555 4606 4650 4793 4998 Size: 54 651 250 406 51 44 143 205 Cuts at: 4998 5025 5093 5120 5162 1391 Size: 27 68 27 42 4072 Fragments arranged by size: 4072 856 651 406 315 250 206 205 149 143 129 72 68 68 54 51 44 42 27 27 8 BbeI G_GCGC'C Cuts at: 3568 3568 Size: 7843 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2176 2352 928 Size: 971 277 176 6419 Fragments arranged by size: 6419 971 277 176 BbvCI CC'TCA_GC Cuts at: 3270 4140 4321 3270 Size: 870 181 6792 Fragments arranged by size: 6792 870 181 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6034 6068 1903 Size: 34 4097 34 3678 Fragments arranged by size: 4097 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2327 3602 5735 6945 2327 Size: 1275 2133 1210 3225 Fragments arranged by size: 3225 2133 1275 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7843 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2652 3409 4278 Size: 107 122 71 631 605 980 757 869 Cuts at: 4278 4766 5115 6454 136 Size: 488 349 1339 1525 Fragments arranged by size: 1525 1339 980 869 757 631 605 488 349 122 107 71 BglII A'GATC_T Cuts at: 7838 7838 Size: 7843 BlpI GC'TnA_GC Cuts at: 3394 4710 4992 5260 3394 Size: 1316 282 268 5977 Fragments arranged by size: 5977 1316 282 268 Bme1580I G_kGCm'C Cuts at: 1790 2611 2674 2803 4606 5093 5904 7060 1790 Size: 821 63 129 1803 487 811 1156 2573 Fragments arranged by size: 2573 1803 1156 821 811 487 129 63 BmgBI CAC'GTC Cuts at: 1318 2956 3711 3753 1318 Size: 1638 755 42 5408 Fragments arranged by size: 5408 1638 755 42 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2187 3246 4577 Size: 416 53 83 399 24 770 1059 1331 Cuts at: 4577 4619 4744 4971 5574 6532 442 Size: 42 125 227 603 958 1753 Fragments arranged by size: 1753 1331 1059 958 770 603 416 399 227 125 83 53 42 24 BmtI G_CTAG'C Cuts at: 1056 3204 1056 Size: 2148 5695 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3132 3264 3132 Size: 132 7711 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2714 3043 3079 3127 3586 4353 4527 4829 Size: 1475 329 36 48 459 767 174 302 Cuts at: 4829 6503 1239 Size: 1674 2579 Fragments arranged by size: 2579 1674 1475 767 459 329 302 174 48 36 Bpu10I CC'TnA_GC Cuts at: 2873 3270 3429 3435 4140 4260 4321 2873 Size: 397 159 6 705 120 61 6395 Fragments arranged by size: 6395 705 397 159 120 61 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4652 4687 5968 6833 7131 4652 Size: 35 1281 865 298 5364 Fragments arranged by size: 5364 1281 865 298 35 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4823 6506 882 Size: 617 3324 1683 2219 Fragments arranged by size: 3324 2219 1683 617 BsaAI yAC'GTr Cuts at: 493 1204 2383 5634 493 Size: 711 1179 3251 2702 Fragments arranged by size: 3251 2702 1179 711 BsaBI GATnn'nnATC Cuts at: 7837 7837 Size: 7843 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2500 3565 3963 5650 Size: 53 83 186 936 967 1065 398 1687 Cuts at: 5650 6032 6667 275 Size: 382 635 1451 Fragments arranged by size: 1687 1451 1065 967 936 635 398 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4438 4869 6276 6674 6948 7095 7506 4438 Size: 431 1407 398 274 147 411 4775 Fragments arranged by size: 4775 1407 431 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2339 2369 2399 2429 3074 Size: 30 165 30 936 30 30 30 645 Cuts at: 3074 3104 3625 3655 3988 4018 4993 5023 1178 Size: 30 521 30 333 30 975 30 3998 Fragments arranged by size: 3998 975 936 645 521 333 165 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3040 3082 3148 3730 3757 3760 3834 4157 Size: 1854 42 66 582 27 3 74 323 Cuts at: 4157 4208 4247 4309 4356 4630 4998 5111 1186 Size: 51 39 62 47 274 368 113 3918 Fragments arranged by size: 3918 1854 582 368 323 274 113 74 66 62 51 47 42 39 27 3 BseYI C'CCAG_C Cuts at: 2036 2292 2527 3019 3037 3453 3520 3531 3684 Size: 256 235 492 18 416 67 11 153 Cuts at: 3684 3778 3904 4384 4561 7046 7542 2036 Size: 94 126 480 177 2485 496 2337 Fragments arranged by size: 2485 2337 496 492 480 416 256 235 177 153 126 94 67 18 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2207 2567 2990 1163 Size: 573 471 360 423 6016 Fragments arranged by size: 6016 573 471 423 360 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2078 2090 2644 5293 6054 7082 Size: 275 186 77 12 554 2649 761 1028 Cuts at: 7082 1540 Size: 2301 Fragments arranged by size: 2649 2301 1028 761 554 275 186 77 12 BsiHKAI G_wGCw'C Cuts at: 2172 2396 2522 2611 3194 4543 4711 5904 5989 Size: 224 126 89 583 1349 168 1193 85 Cuts at: 5989 7060 2172 Size: 1071 2955 Fragments arranged by size: 2955 1349 1193 1071 583 224 168 126 89 85 BsiWI C'GTAC_G Cuts at: 2620 2620 Size: 7843 BsmI GAATG_Cn' Cuts at: 3291 5354 5447 3291 Size: 2063 93 5687 Fragments arranged by size: 5687 2063 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3061 3717 3742 3061 Size: 656 25 7162 Fragments arranged by size: 7162 656 25 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2172 2247 2396 2522 2602 2611 2674 Size: 399 382 75 149 126 80 9 63 Cuts at: 2674 2803 2871 2879 3194 3248 3899 4149 4543 Size: 129 68 8 315 54 651 250 394 Cuts at: 4543 4555 4606 4650 4711 4793 4998 5025 5093 Size: 12 51 44 61 82 205 27 68 Cuts at: 5093 5120 5162 5904 5989 7060 1391 Size: 27 42 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 651 399 394 382 315 250 205 149 129 126 85 82 80 75 68 68 63 61 54 51 44 42 27 27 12 9 8 BspEI T'CCGG_A Cuts at: 4869 7506 4869 Size: 2637 5206 BspHI T'CATG_A Cuts at: 5732 7462 5732 Size: 1730 6113 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3647 5198 844 Size: 2803 1551 3489 Fragments arranged by size: 3489 2803 1551 BsrBI CCG'CTC Cuts at: 1394 1823 2250 2424 2512 3033 3795 4813 5730 Size: 429 427 174 88 521 762 1018 917 Cuts at: 5730 1394 Size: 3507 Fragments arranged by size: 3507 1018 917 762 521 429 427 174 88 BsrDI GCAATG_nn' Cuts at: 65 1248 6338 6512 65 Size: 1183 5090 174 1396 Fragments arranged by size: 5090 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2089 2781 4438 6487 6674 1700 Size: 110 279 692 1657 2049 187 2869 Fragments arranged by size: 2869 2049 1657 692 279 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7843 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7843 BssSI C'ACGA_G Cuts at: 5903 6915 5903 Size: 1012 6831 BstAPI GCAn_nnn'nTGC Cuts at: 794 2099 2545 2615 2861 4124 4519 794 Size: 1305 446 70 246 1263 395 4118 Fragments arranged by size: 4118 1305 1263 446 395 246 70 BstBI TT'CG_AA Cuts at: 6647 6647 Size: 7843 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7843 BstXI CCAn_nnnn'nTGG Cuts at: 3652 3652 Size: 7843 BstYI r'GATC_y Cuts at: 1972 4156 5173 5540 5925 5942 7394 7480 7492 Size: 2184 1017 367 385 17 1452 86 12 Cuts at: 7492 7838 1972 Size: 346 1977 Fragments arranged by size: 2184 1977 1452 1017 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2564 2659 4191 4642 4728 4917 2564 Size: 95 1532 451 86 189 5490 Fragments arranged by size: 5490 1532 451 189 95 86 BtgI C'CryG_G Cuts at: 513 1065 2004 2299 2787 3572 4209 4533 4739 Size: 552 939 295 488 785 637 324 206 Cuts at: 4739 513 Size: 3617 Fragments arranged by size: 3617 939 785 637 552 488 324 295 206 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7104 Fragments arranged by size: 7104 683 56 BtsI GCAGTG_nn' Cuts at: 2166 2838 3241 3264 3307 3405 4007 4592 5359 Size: 672 403 23 43 98 602 585 767 Cuts at: 5359 6153 6173 2166 Size: 794 20 3836 Fragments arranged by size: 3836 794 767 672 602 585 403 98 43 23 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7843 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3792 3827 4441 4476 529 Size: 35 3228 35 614 35 3896 Fragments arranged by size: 3896 3228 614 35 35 35 DraI TTT'AAA Cuts at: 5147 5147 Size: 7843 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3833 6850 809 Size: 507 2517 3017 1802 Fragments arranged by size: 3017 2517 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2087 2140 2641 Size: 54 1425 50 275 18 257 53 501 Cuts at: 2641 2779 2880 3012 3316 3464 4251 4416 4683 Size: 138 101 132 304 148 787 165 267 Cuts at: 4683 4767 4889 5139 5290 6179 7808 8 Size: 84 122 250 151 889 1629 43 Fragments arranged by size: 1629 1425 889 787 501 304 275 267 257 250 165 151 148 138 132 122 101 84 54 53 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2087 2641 5290 1537 Size: 275 275 554 2649 4090 Fragments arranged by size: 4090 2649 554 275 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2211 3720 3748 5773 1353 Size: 858 1509 28 2025 3423 Fragments arranged by size: 3423 2025 1509 858 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2530 2714 2720 4365 4390 5075 Size: 1064 138 941 184 6 1645 25 685 Cuts at: 5075 6430 6804 6950 387 Size: 1355 374 146 1280 Fragments arranged by size: 1645 1355 1280 1064 941 685 374 184 146 138 25 6 EcoICRI GAG'CTC Cuts at: 2394 3192 2394 Size: 798 7045 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2714 3043 3079 3127 3362 3586 4260 Size: 393 1082 329 36 48 235 224 674 Cuts at: 4260 4353 4527 4829 5906 6503 7290 7558 1239 Size: 93 174 302 1077 597 787 268 1524 Fragments arranged by size: 1524 1082 1077 787 674 597 393 329 302 268 235 224 174 93 48 36 EcoO109I rG'GnC_Cy Cuts at: 2032 2135 2670 2800 2858 3649 3659 3729 4201 Size: 103 535 130 58 791 10 70 472 Cuts at: 4201 4271 4602 4603 4900 2032 Size: 70 331 1 297 4975 Fragments arranged by size: 4975 791 535 472 331 297 130 103 70 70 58 10 1 EcoRI G'AATT_C Cuts at: 5152 5530 5152 Size: 378 7465 FspI TGC'GCA Cuts at: 2984 6349 2984 Size: 3365 4478 HaeII r_GCGC'y Cuts at: 1678 2693 2718 2743 3046 3568 6990 1678 Size: 1015 25 25 303 522 3422 2531 Fragments arranged by size: 3422 2531 1015 522 303 25 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2339 2371 2399 2431 4106 Size: 32 135 32 767 32 28 32 1675 Cuts at: 4106 4138 6484 6516 6558 6590 1373 Size: 32 2346 32 42 32 2626 Fragments arranged by size: 2626 2346 1675 767 135 42 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 5187 5433 669 Size: 988 281 3249 246 3079 Fragments arranged by size: 3249 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7843 HpaI GTT'AAC Cuts at: 5433 5433 Size: 7843 KasI G'GCGC_C Cuts at: 3564 3564 Size: 7843 KpnI G_GTAC'C Cuts at: 2796 3218 3539 5168 2796 Size: 422 321 1629 5471 Fragments arranged by size: 5471 1629 422 321 MfeI C'AATT_G Cuts at: 5442 5442 Size: 7843 MluI A'CGCG_T Cuts at: 7719 7719 Size: 7843 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2482 3150 4687 6957 7141 1305 Size: 1177 668 1537 2270 184 2007 Fragments arranged by size: 2270 2007 1537 1177 668 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2142 4253 4418 10 Size: 54 1425 343 310 2111 165 3435 Fragments arranged by size: 3435 2111 1425 343 310 165 54 MslI CAynn'nnrTG Cuts at: 518 1166 2127 3134 5801 6160 6319 518 Size: 648 961 1007 2667 359 159 2042 Fragments arranged by size: 2667 2042 1007 961 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2040 2973 2997 3388 3457 3531 3908 4807 Size: 434 933 24 391 69 74 377 899 Cuts at: 4807 4849 4888 5083 5259 5936 7084 7329 1606 Size: 42 39 195 176 677 1148 245 2120 Fragments arranged by size: 2120 1148 933 899 677 434 391 377 245 195 176 74 69 42 39 24 NaeI GCC'GGC Cuts at: 2091 2091 Size: 7843 NarI GG'CG_CC Cuts at: 3565 3565 Size: 7843 NcoI C'CATG_G Cuts at: 513 1065 2004 2299 4209 513 Size: 552 939 295 1910 4147 Fragments arranged by size: 4147 1910 939 552 295 NdeI CA'TA_TG Cuts at: 387 387 Size: 7843 NgoMIV G'CCGG_C Cuts at: 2089 2089 Size: 7843 NheI G'CTAG_C Cuts at: 1052 3200 1052 Size: 2148 5695 NotI GC'GGCC_GC Cuts at: 5290 5290 Size: 7843 NspI r_CATG'y Cuts at: 1132 1229 2546 2933 4415 5201 6746 1132 Size: 97 1317 387 1482 786 1545 2229 Fragments arranged by size: 2229 1545 1482 1317 786 387 97 PasI CC'CwG_GG Cuts at: 1348 3226 3725 3820 4406 4470 1348 Size: 1878 499 95 586 64 4721 Fragments arranged by size: 4721 1878 586 499 95 64 PciI A'CATG_T Cuts at: 6742 6742 Size: 7843 PflMI CCAn_nnn'nTGG Cuts at: 1108 3531 1108 Size: 2423 5420 PfoI T'CCnGG_A Cuts at: 2327 4543 2327 Size: 2216 5627 PmeI GTTT'AAAC Cuts at: 5147 5147 Size: 7843 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5912 5944 7425 7457 5912 Size: 32 1481 32 6298 Fragments arranged by size: 6298 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3659 4201 3659 Size: 542 7301 PshAI GACnn'nnGTC Cuts at: 1613 3966 1613 Size: 2353 5490 PsiI TTA'TAA Cuts at: 5413 5413 Size: 7843 PspOMI G'GGCC_C Cuts at: 2670 2799 4602 5089 2670 Size: 129 1803 487 5424 Fragments arranged by size: 5424 1803 487 129 PspXI vC'TCGA_Gb Cuts at: 3613 4986 3613 Size: 1373 6470 PstI C_TGCA'G Cuts at: 830 2161 2366 2688 2739 2866 2978 3002 4014 Size: 1331 205 322 51 127 112 24 1012 Cuts at: 4014 4270 4515 4679 4806 5082 5195 830 Size: 256 245 164 127 276 113 3478 Fragments arranged by size: 3478 1331 1012 322 276 256 245 205 164 127 127 113 112 51 24 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7843 PvuII CAG'CTG Cuts at: 3457 3531 3908 4807 5083 3457 Size: 74 377 899 276 6217 Fragments arranged by size: 6217 899 377 276 74 RsrII CG'GwC_CG Cuts at: 4660 4721 4660 Size: 61 7782 SacI G_AGCT'C Cuts at: 2396 3194 2396 Size: 798 7045 SalI G'TCGA_C Cuts at: 1936 5185 1936 Size: 3249 4594 SapI GCTCTTCn'nnn_ Cuts at: 2211 2211 Size: 7843 SbfI CC_TGCA'GG Cuts at: 2688 4270 5195 2688 Size: 1582 925 5336 Fragments arranged by size: 5336 1582 925 ScaI AGT'ACT Cuts at: 6091 7803 6091 Size: 1712 6131 SfcI C'TryA_G Cuts at: 826 1045 2157 2362 2684 2735 2862 2946 2974 Size: 219 1112 205 322 51 127 84 28 Cuts at: 2974 2998 4010 4266 4447 4511 4675 4802 5078 Size: 24 1012 256 181 64 164 127 276 Cuts at: 5078 5191 6326 7007 7198 826 Size: 113 1135 681 191 1471 Fragments arranged by size: 1471 1135 1112 1012 681 322 276 256 219 205 191 181 164 127 127 113 84 64 51 28 24 SfoI GGC'GCC Cuts at: 3566 3566 Size: 7843 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7843 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7843 SmaI CCC'GGG Cuts at: 1872 2048 2597 2675 2798 4350 5115 5170 1872 Size: 176 549 78 123 1552 765 55 4545 Fragments arranged by size: 4545 1552 765 549 176 123 78 55 SmlI C'TyrA_G Cuts at: 820 1017 1943 3613 4667 4702 4986 5947 6848 Size: 197 926 1670 1054 35 284 961 901 Cuts at: 6848 7110 820 Size: 262 1553 Fragments arranged by size: 1670 1553 1054 961 926 901 284 262 197 35 SnaBI TAC'GTA Cuts at: 493 5634 493 Size: 5141 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7843 SphI G_CATG'C Cuts at: 1132 5201 1132 Size: 4069 3774 SspI AAT'ATT Cuts at: 5 52 5767 5 Size: 47 5715 2081 Fragments arranged by size: 5715 2081 47 StuI AGG'CCT Cuts at: 1517 3275 4673 4698 1517 Size: 1758 1398 25 4662 Fragments arranged by size: 4662 1758 1398 25 StyI C'CwwG_G Cuts at: 513 1065 2004 2103 2299 2965 3645 3852 4209 Size: 552 939 99 196 666 680 207 357 Cuts at: 4209 4785 5282 513 Size: 576 497 3074 Fragments arranged by size: 3074 939 680 666 576 552 497 357 207 196 99 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2324 3099 3484 3529 3812 4102 4526 Size: 1255 744 775 385 45 283 290 424 Cuts at: 4526 5048 5870 5887 6040 6225 6670 325 Size: 522 822 17 153 185 445 1498 Fragments arranged by size: 1498 1255 822 775 744 522 445 424 385 290 283 185 153 45 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6089 7801 96 Size: 274 80 33 51 1443 4112 1712 138 Fragments arranged by size: 4112 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2560 2592 3074 3104 3106 3136 3625 3657 7803 Size: 32 482 30 2 30 489 32 4146 Cuts at: 7803 7835 2560 Size: 32 2568 Fragments arranged by size: 4146 2568 489 482 32 32 32 30 30 2 Tth111I GACn'n_nGTC Cuts at: 1316 2180 2753 1316 Size: 864 573 6406 Fragments arranged by size: 6406 864 573 XbaI T'CTAG_A Cuts at: 5179 5179 Size: 7843 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2380 3233 3528 4564 2380 Size: 853 295 1036 5659 Fragments arranged by size: 5659 1036 853 295 XhoI C'TCGA_G Cuts at: 1943 3613 4986 1943 Size: 1670 1373 4800 Fragments arranged by size: 4800 1670 1373 XmaI C'CCGG_G Cuts at: 1870 2046 2595 2673 2796 4348 5113 5168 1870 Size: 176 549 78 123 1552 765 55 4545 Fragments arranged by size: 4545 1552 765 549 176 123 78 55 XmnI GAAnn'nnTTC Cuts at: 5972 5972 Size: 7843 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5651 276 Size: 53 83 186 936 4117 2468 Fragments arranged by size: 4117 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BfrBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PsrI SacII SanDI SexAI SfiI SrfI SwaI