(Circular) (Six-base) MAPSORT of: pFN21AA1521.seq Check: 6441 from: 1 to: 9139 pFN21AA1521 9139 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4025 4025 Size: 9139 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3981 6949 278 Size: 53 83 186 936 2445 2968 2468 Fragments arranged by size: 2968 2468 2445 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5665 6323 6482 1937 Size: 3728 658 159 4594 Fragments arranged by size: 4594 3728 658 159 Acc65I G'GTAC_C Cuts at: 6460 6460 Size: 9139 AclI AA'CG_TT Cuts at: 1563 4945 7266 7639 1563 Size: 3382 2321 373 3063 Fragments arranged by size: 3382 3063 2321 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2214 2438 2521 2583 3093 3753 4905 5526 Size: 582 224 83 62 510 660 1152 621 Cuts at: 5526 6074 7202 8586 8854 1632 Size: 548 1128 1384 268 1917 Fragments arranged by size: 1917 1384 1152 1128 660 621 582 548 510 268 224 83 62 AflII C'TTAA_G Cuts at: 820 1017 2108 820 Size: 197 1091 7851 Fragments arranged by size: 7851 1091 197 AflIII A'CryG_T Cuts at: 1203 4053 8038 9015 1203 Size: 2850 3985 977 1327 Fragments arranged by size: 3985 2850 1327 977 AgeI A'CCGG_T Cuts at: 7970 7970 Size: 9139 AhdI GACnn_n'nnGTC Cuts at: 7868 7868 Size: 9139 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3488 4047 1184 Size: 2304 559 6276 Fragments arranged by size: 6276 2304 559 AlwNI CAG_nnn'CTG Cuts at: 4040 5312 6882 8454 4040 Size: 1272 1570 1572 4725 Fragments arranged by size: 4725 1572 1570 1272 ApaLI G'TGCA_C Cuts at: 5073 5250 7196 8352 5073 Size: 177 1946 1156 5860 Fragments arranged by size: 5860 1946 1156 177 ApoI r'AATT_y Cuts at: 1493 2229 2297 2477 2795 3259 3906 5804 6410 Size: 736 68 180 318 464 647 1898 606 Cuts at: 6410 6448 6674 6826 8935 1493 Size: 38 226 152 2109 1697 Fragments arranged by size: 2109 1898 1697 736 647 606 464 318 226 180 152 68 38 AseI AT'TA_AT Cuts at: 160 1032 3003 5514 7693 160 Size: 872 1971 2511 2179 1606 Fragments arranged by size: 2511 2179 1971 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9139 AvaI C'yCGr_G Cuts at: 1870 1943 4855 6174 6464 1870 Size: 73 2912 1319 290 4545 Fragments arranged by size: 4545 2912 1319 290 73 AvrII C'CTAG_G Cuts at: 2504 2504 Size: 9139 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4245 4278 4245 Size: 33 9106 BamHI G'GATC_C Cuts at: 6469 6836 6469 Size: 367 8772 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4433 6460 7915 618 Size: 325 217 627 2646 2027 1455 1842 Fragments arranged by size: 2646 2027 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2723 4712 5638 6088 6458 1391 Size: 1332 1989 926 450 370 4072 Fragments arranged by size: 4072 1989 1332 926 450 370 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3177 4359 4491 5472 928 Size: 971 1278 1182 132 981 4595 Fragments arranged by size: 4595 1278 1182 981 971 132 BbvCI CC'TCA_GC Cuts at: 3393 5091 5616 3393 Size: 1698 525 6916 Fragments arranged by size: 6916 1698 525 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5831 5865 7330 7364 1903 Size: 34 3894 34 1465 34 3678 Fragments arranged by size: 3894 3678 1465 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7031 8241 7031 Size: 1210 7929 BclI T'GATC_A Cuts at: 1548 1548 Size: 9139 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7750 136 Size: 107 122 71 631 605 6078 1525 Fragments arranged by size: 6078 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4361 9134 4361 Size: 4773 4366 BlpI GC'TnA_GC Cuts at: 6556 6556 Size: 9139 Bme1580I G_kGCm'C Cuts at: 1790 4438 5077 5254 7200 8356 1790 Size: 2648 639 177 1946 1156 2573 Fragments arranged by size: 2648 2573 1946 1156 639 177 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4244 5133 5408 Size: 416 53 83 399 24 2827 889 275 Cuts at: 5408 6870 7828 442 Size: 1462 958 1753 Fragments arranged by size: 2827 1753 1462 958 889 416 399 275 83 53 24 BmtI G_CTAG'C Cuts at: 1056 6357 1056 Size: 5301 3838 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2698 2700 2737 4390 5400 2698 Size: 2 37 1653 1010 6437 Fragments arranged by size: 6437 1653 1010 37 2 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2373 3303 3387 3399 3567 3988 4238 4629 Size: 1134 930 84 12 168 421 250 391 Cuts at: 4629 4779 6276 6387 7799 1239 Size: 150 1497 111 1412 2579 Fragments arranged by size: 2579 1497 1412 1134 930 421 391 250 168 150 111 84 12 Bpu10I CC'TnA_GC Cuts at: 3393 3893 5082 5091 5616 5853 6227 3393 Size: 500 1189 9 525 237 374 6305 Fragments arranged by size: 6305 1189 525 500 374 237 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2027 2323 3495 4855 5798 7264 8129 8427 2027 Size: 296 1172 1360 943 1466 865 298 2739 Fragments arranged by size: 2739 1466 1360 1172 943 865 298 296 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3164 3956 4729 7802 882 Size: 617 1665 792 773 3073 2219 Fragments arranged by size: 3073 2219 1665 792 773 617 BsaAI yAC'GTr Cuts at: 493 1204 6930 493 Size: 711 5726 2702 Fragments arranged by size: 5726 2702 711 BsaBI GATnn'nnATC Cuts at: 2640 4014 5529 9133 2640 Size: 1374 1515 3604 2646 Fragments arranged by size: 3604 2646 1515 1374 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3978 4765 6946 7328 Size: 53 83 186 936 2445 787 2181 382 Cuts at: 7328 7963 275 Size: 635 1451 Fragments arranged by size: 2445 2181 1451 936 787 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4731 5973 7572 7970 8244 8391 8802 4731 Size: 1242 1599 398 274 147 411 5068 Fragments arranged by size: 5068 1599 1242 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3774 3804 4438 4468 4930 Size: 30 165 30 2371 30 634 30 462 Cuts at: 4930 4960 1178 Size: 30 5357 Fragments arranged by size: 5357 2371 634 462 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2329 2533 4214 4443 4858 4890 6386 1186 Size: 1143 204 1681 229 415 32 1496 3939 Fragments arranged by size: 3939 1681 1496 1143 415 229 204 32 BseYI C'CCAG_C Cuts at: 3573 4283 4751 5057 8342 8838 3573 Size: 710 468 306 3285 496 3874 Fragments arranged by size: 3874 3285 710 496 468 306 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3183 4093 4390 4815 6117 6206 6350 Size: 573 1447 910 297 425 1302 89 144 Cuts at: 6350 1163 Size: 3952 Fragments arranged by size: 3952 1447 1302 910 573 425 297 144 89 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6589 7350 8378 1540 Size: 275 186 4588 761 1028 2301 Fragments arranged by size: 4588 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4712 5077 5254 6088 6209 7200 7285 8356 4712 Size: 365 177 834 121 991 85 1071 5495 Fragments arranged by size: 5495 1071 991 834 365 177 121 85 BsmI GAATG_Cn' Cuts at: 4661 4675 5122 6650 6743 4661 Size: 14 447 1528 93 7057 Fragments arranged by size: 7057 1528 447 93 14 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2723 4438 4712 5077 5254 5638 6088 Size: 399 933 1715 274 365 177 384 450 Cuts at: 6088 6209 6458 7200 7285 8356 1391 Size: 121 249 742 85 1071 2174 Fragments arranged by size: 2174 1715 1071 933 742 450 399 384 365 274 249 177 121 85 BspEI T'CCGG_A Cuts at: 4731 8802 4731 Size: 4071 5068 BspHI T'CATG_A Cuts at: 5182 6037 7028 8758 5182 Size: 855 991 1730 5563 Fragments arranged by size: 5563 1730 991 855 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3022 4025 6494 844 Size: 2178 1003 2469 3489 Fragments arranged by size: 3489 2469 2178 1003 BsrBI CCG'CTC Cuts at: 1394 1823 6260 7026 1394 Size: 429 4437 766 3507 Fragments arranged by size: 4437 3507 766 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2922 3066 5157 5895 7634 7808 65 Size: 1183 1674 144 2091 738 1739 174 1396 Fragments arranged by size: 2091 1739 1674 1396 1183 738 174 144 BsrFI r'CCGG_y Cuts at: 1700 1810 4761 7783 7970 1700 Size: 110 2951 3022 187 2869 Fragments arranged by size: 3022 2951 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2865 96 Size: 2769 6370 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9139 BssSI C'ACGA_G Cuts at: 7199 8211 7199 Size: 1012 8127 BstAPI GCAn_nnn'nTGC Cuts at: 794 2144 4040 794 Size: 1350 1896 5893 Fragments arranged by size: 5893 1896 1350 BstBI TT'CG_AA Cuts at: 4699 7943 4699 Size: 3244 5895 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9139 BstXI CCAn_nnnn'nTGG Cuts at: 2233 2233 Size: 9139 BstYI r'GATC_y Cuts at: 1972 2656 3082 4361 4570 6469 6836 7221 7238 Size: 684 426 1279 209 1899 367 385 17 Cuts at: 7238 8690 8776 8788 9134 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1899 1452 1279 684 426 385 367 346 209 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3559 3559 Size: 9139 BtgI C'CryG_G Cuts at: 513 1065 2004 3670 6122 513 Size: 552 939 1666 2452 3530 Fragments arranged by size: 3530 2452 1666 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4539 4917 487 Size: 683 56 3313 378 4709 Fragments arranged by size: 4709 3313 683 378 56 BtsI GCAGTG_nn' Cuts at: 3171 3715 5276 6194 6655 7449 7469 3171 Size: 544 1561 918 461 794 20 4841 Fragments arranged by size: 4841 1561 918 794 544 461 20 ClaI AT'CG_AT Cuts at: 1554 3433 5743 6426 1554 Size: 1879 2310 683 4267 Fragments arranged by size: 4267 2310 1879 683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2760 2795 6297 6332 529 Size: 35 2196 35 3502 35 3336 Fragments arranged by size: 3502 3336 2196 35 35 35 DraI TTT'AAA Cuts at: 2551 6443 2551 Size: 3892 5247 DraIII CAC_nnn'GTG Cuts at: 2384 2384 Size: 9139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8146 809 Size: 507 6830 1802 Fragments arranged by size: 6830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3342 4479 5736 Size: 54 1425 50 275 18 1512 1137 1257 Cuts at: 5736 5949 6125 6234 6586 7475 9104 8 Size: 213 176 109 352 889 1629 43 Fragments arranged by size: 1629 1512 1425 1257 1137 889 352 275 213 176 109 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6586 1537 Size: 275 4774 4090 Fragments arranged by size: 4774 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3069 3263 3534 4773 6093 7069 1353 Size: 1716 194 271 1239 1320 976 3423 Fragments arranged by size: 3423 1716 1320 1239 976 271 194 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7726 8100 8246 387 Size: 1064 138 6137 374 146 1280 Fragments arranged by size: 6137 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4710 6086 4710 Size: 1376 7763 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2214 2373 2438 2521 2583 3093 3303 Size: 393 582 159 65 83 62 510 210 Cuts at: 3303 3387 3399 3567 3753 3988 4238 4629 4779 Size: 84 12 168 186 235 250 391 150 Cuts at: 4779 4905 5526 6074 6276 6387 7202 7799 8586 Size: 126 621 548 202 111 815 597 787 Cuts at: 8586 8854 1239 Size: 268 1524 Fragments arranged by size: 1524 815 787 621 597 582 548 510 393 391 268 250 235 210 202 186 168 159 150 126 111 84 83 65 62 12 EcoNI CCTnn'n_nnAGG Cuts at: 2045 2453 4193 2045 Size: 408 1740 6991 Fragments arranged by size: 6991 1740 408 EcoO109I rG'GnC_Cy Cuts at: 3569 3607 3737 3809 4180 5024 3569 Size: 38 130 72 371 844 7684 Fragments arranged by size: 7684 844 371 130 72 38 EcoRI G'AATT_C Cuts at: 2297 5804 6410 6448 6826 2297 Size: 3507 606 38 378 4610 Fragments arranged by size: 4610 3507 606 378 38 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4161 4695 5948 6007 4161 Size: 534 1253 59 7293 Fragments arranged by size: 7293 1253 534 59 FspI TGC'GCA Cuts at: 7645 7645 Size: 9139 HaeII r_GCGC'y Cuts at: 1678 8286 1678 Size: 6608 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2305 2337 2842 2874 3776 Size: 32 135 32 733 32 505 32 902 Cuts at: 3776 3808 4346 4378 4438 4470 4481 4513 4706 Size: 32 538 32 60 32 11 32 193 Cuts at: 4706 4738 4898 4930 5021 5053 6061 6093 7780 Size: 32 160 32 91 32 1008 32 1687 Cuts at: 7780 7812 7854 7886 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1687 1008 902 733 538 505 193 160 135 91 60 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 5666 6483 6729 669 Size: 988 281 3728 817 246 3079 Fragments arranged by size: 3728 3079 988 817 281 246 HindIII A'AGCT_T Cuts at: 748 2196 4143 748 Size: 1448 1947 5744 Fragments arranged by size: 5744 1947 1448 HpaI GTT'AAC Cuts at: 6729 6729 Size: 9139 KpnI G_GTAC'C Cuts at: 6464 6464 Size: 9139 MfeI C'AATT_G Cuts at: 2267 2419 6738 2267 Size: 152 4319 4668 Fragments arranged by size: 4668 4319 152 MluI A'CGCG_T Cuts at: 9015 9015 Size: 9139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 8253 8437 1305 Size: 6948 184 2007 Fragments arranged by size: 6948 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4481 5738 5951 6127 6236 Size: 54 1425 343 2649 1257 213 176 109 Cuts at: 6236 10 Size: 2913 Fragments arranged by size: 2913 2649 1425 1257 343 213 176 109 54 MslI CAynn'nnrTG Cuts at: 518 1166 3470 7097 7456 7615 518 Size: 648 2304 3627 359 159 2042 Fragments arranged by size: 3627 2304 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2117 3844 4444 4649 4755 4799 5837 5947 Size: 511 1727 600 205 106 44 1038 110 Cuts at: 5947 6555 7232 8380 8625 1606 Size: 608 677 1148 245 2120 Fragments arranged by size: 2120 1727 1148 1038 677 608 600 511 245 205 110 106 44 NcoI C'CATG_G Cuts at: 513 1065 2004 3670 6122 513 Size: 552 939 1666 2452 3530 Fragments arranged by size: 3530 2452 1666 939 552 NdeI CA'TA_TG Cuts at: 387 3489 5906 387 Size: 3102 2417 3620 Fragments arranged by size: 3620 3102 2417 NheI G'CTAG_C Cuts at: 1052 6353 1052 Size: 5301 3838 NotI GC'GGCC_GC Cuts at: 6586 6586 Size: 9139 NruI TCG'CGA Cuts at: 6024 6024 Size: 9139 NspI r_CATG'y Cuts at: 1132 1229 2225 3619 4057 6497 8042 1132 Size: 97 996 1394 438 2440 1545 2229 Fragments arranged by size: 2440 2229 1545 1394 996 438 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9139 PciI A'CATG_T Cuts at: 4053 8038 4053 Size: 3985 5154 PflMI CCAn_nnn'nTGG Cuts at: 1108 4625 1108 Size: 3517 5622 PfoI T'CCnGG_A Cuts at: 3522 4581 4768 3522 Size: 1059 187 7893 Fragments arranged by size: 7893 1059 187 PmeI GTTT'AAAC Cuts at: 6443 6443 Size: 9139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7208 7240 8721 8753 7208 Size: 32 1481 32 7594 Fragments arranged by size: 7594 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3569 3607 3737 3809 3569 Size: 38 130 72 8899 Fragments arranged by size: 8899 130 72 38 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9139 PsiI TTA'TAA Cuts at: 6165 6709 6165 Size: 544 8595 PstI C_TGCA'G Cuts at: 830 2959 3710 4045 4804 5283 5874 6491 830 Size: 2129 751 335 759 479 591 617 3478 Fragments arranged by size: 3478 2129 759 751 617 591 479 335 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9139 PvuII CAG'CTG Cuts at: 2117 3844 4444 4649 4755 4799 5837 5947 2117 Size: 1727 600 205 106 44 1038 110 5309 Fragments arranged by size: 5309 1727 1038 600 205 110 106 44 SacI G_AGCT'C Cuts at: 4712 6088 4712 Size: 1376 7763 SalI G'TCGA_C Cuts at: 1936 5664 6481 1936 Size: 3728 817 4594 Fragments arranged by size: 4594 3728 817 SapI GCTCTTCn'nnn_ Cuts at: 4773 6093 4773 Size: 1320 7819 SbfI CC_TGCA'GG Cuts at: 6491 6491 Size: 9139 ScaI AGT'ACT Cuts at: 7387 9099 7387 Size: 1712 7427 SexAI A'CCwGG_T Cuts at: 6027 6027 Size: 9139 SfcI C'TryA_G Cuts at: 826 1045 2955 3235 3441 3706 3839 4041 4800 Size: 219 1910 280 206 265 133 202 759 Cuts at: 4800 5279 5623 5675 5870 6487 7622 8303 8494 Size: 479 344 52 195 617 1135 681 191 Cuts at: 8494 826 Size: 1471 Fragments arranged by size: 1910 1471 1135 759 681 617 479 344 280 265 219 206 202 195 191 133 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9139 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9139 SmaI CCC'GGG Cuts at: 1872 6176 6466 1872 Size: 4304 290 4545 Fragments arranged by size: 4545 4304 290 SmlI C'TyrA_G Cuts at: 820 1017 1943 2042 2108 2302 3510 4870 5777 Size: 197 926 99 66 194 1208 1360 907 Cuts at: 5777 7243 8144 8406 820 Size: 1466 901 262 1553 Fragments arranged by size: 1553 1466 1360 1208 926 907 901 262 197 194 99 66 SnaBI TAC'GTA Cuts at: 493 6930 493 Size: 6437 2702 SpeI A'CTAG_T Cuts at: 152 4403 4693 152 Size: 4251 290 4598 Fragments arranged by size: 4598 4251 290 SphI G_CATG'C Cuts at: 1132 6497 1132 Size: 5365 3774 SspI AAT'ATT Cuts at: 5 52 4006 7063 5 Size: 47 3954 3057 2081 Fragments arranged by size: 3954 3057 2081 47 StuI AGG'CCT Cuts at: 1517 4296 1517 Size: 2779 6360 StyI C'CwwG_G Cuts at: 513 1065 2004 2504 3103 3670 4931 6122 6578 Size: 552 939 500 599 567 1261 1191 456 Cuts at: 6578 513 Size: 3074 Fragments arranged by size: 3074 1261 1191 939 599 567 552 500 456 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5225 6447 7166 7183 7336 7521 7966 Size: 1255 3645 1222 719 17 153 185 445 Cuts at: 7966 325 Size: 1498 Fragments arranged by size: 3645 1498 1255 1222 719 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2104 2865 2921 Size: 274 80 33 51 1443 127 761 56 Cuts at: 2921 3971 5168 7385 9097 96 Size: 1050 1197 2217 1712 138 Fragments arranged by size: 2217 1712 1443 1197 1050 761 274 138 127 80 56 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3774 3806 5038 5070 6142 6174 9099 9131 3774 Size: 32 1232 32 1072 32 2925 32 3782 Fragments arranged by size: 3782 2925 1232 1072 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6248 1316 Size: 4932 4207 XbaI T'CTAG_A Cuts at: 6475 6475 Size: 9139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3475 3475 Size: 9139 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9139 XmaI C'CCGG_G Cuts at: 1870 6174 6464 1870 Size: 4304 290 4545 Fragments arranged by size: 4545 4304 290 XmnI GAAnn'nnTTC Cuts at: 2986 5808 5865 5931 7268 2986 Size: 2822 57 66 1337 4857 Fragments arranged by size: 4857 2822 1337 66 57 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3979 6947 276 Size: 53 83 186 936 2445 2968 2468 Fragments arranged by size: 2968 2468 2445 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI