(Circular) (Six-base) MAPSORT of: pFN21AA1496.seq Check: 444 from: 1 to: 7789 pFN21AA1496 7789 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3259 3446 3259 Size: 187 7602 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5599 278 Size: 53 83 186 936 4063 2468 Fragments arranged by size: 4063 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5132 1937 Size: 3195 4594 Acc65I G'GTAC_C Cuts at: 5110 5110 Size: 7789 AclI AA'CG_TT Cuts at: 1563 5916 6289 1563 Size: 4353 373 3063 Fragments arranged by size: 4353 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2336 4110 4140 4200 4631 4863 4998 5852 Size: 704 1774 30 60 431 232 135 854 Cuts at: 5852 7236 7504 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1774 1384 854 704 431 268 232 135 60 30 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7592 AflIII A'CryG_T Cuts at: 1203 2588 6688 7665 1203 Size: 1385 4100 977 1327 Fragments arranged by size: 4100 1385 1327 977 AgeI A'CCGG_T Cuts at: 6620 6620 Size: 7789 AhdI GACnn_n'nnGTC Cuts at: 4839 6518 4839 Size: 1679 6110 AleI CACnn'nnGTG Cuts at: 1166 2591 3947 4757 1166 Size: 1425 1356 810 4198 Fragments arranged by size: 4198 1425 1356 810 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7789 AlwNI CAG_nnn'CTG Cuts at: 2402 3953 3986 4178 5532 7104 2402 Size: 1551 33 192 1354 1572 3087 Fragments arranged by size: 3087 1572 1551 1354 192 33 ApaI G_GGCC'C Cuts at: 3625 3625 Size: 7789 ApaLI G'TGCA_C Cuts at: 5846 7002 5846 Size: 1156 6633 ApoI r'AATT_y Cuts at: 1493 2314 2371 2905 3369 4019 5098 5324 5476 Size: 821 57 534 464 650 1079 226 152 Cuts at: 5476 7585 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1079 821 650 534 464 226 152 57 AseI AT'TA_AT Cuts at: 160 1032 2265 2781 6343 160 Size: 872 1233 516 3562 1606 Fragments arranged by size: 3562 1606 1233 872 516 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7789 AvaI C'yCGr_G Cuts at: 1870 1943 2619 5114 1870 Size: 73 676 2495 4545 Fragments arranged by size: 4545 2495 676 73 BamHI G'GATC_C Cuts at: 4168 4248 5006 5119 5486 4168 Size: 80 758 113 367 6471 Fragments arranged by size: 6471 758 367 113 80 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2541 3110 3517 5110 6565 Size: 325 217 627 754 569 407 1593 1455 Cuts at: 6565 618 Size: 1842 Fragments arranged by size: 1842 1593 1455 754 627 569 407 325 217 BanII G_rGCy'C Cuts at: 1391 2633 3207 3625 5108 1391 Size: 1242 574 418 1483 4072 Fragments arranged by size: 4072 1483 1242 574 418 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3977 928 Size: 971 2078 4740 Fragments arranged by size: 4740 2078 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5980 6014 1903 Size: 34 4043 34 3678 Fragments arranged by size: 4043 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2278 5681 6891 2278 Size: 3403 1210 3176 Fragments arranged by size: 3403 3176 1210 BclI T'GATC_A Cuts at: 1548 3693 1548 Size: 2145 5644 BfrBI ATG'CAT Cuts at: 2895 2895 Size: 7789 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6400 136 Size: 107 122 71 631 605 4728 1525 Fragments arranged by size: 4728 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7784 7784 Size: 7789 BlpI GC'TnA_GC Cuts at: 3201 5206 3201 Size: 2005 5784 Bme1580I G_kGCm'C Cuts at: 1790 2544 3115 3322 3625 5850 7006 1790 Size: 754 571 207 303 2225 1156 2573 Fragments arranged by size: 2573 2225 1156 754 571 303 207 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7789 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3878 4370 4520 Size: 416 53 83 399 24 2461 492 150 Cuts at: 4520 4707 5520 6478 442 Size: 187 813 958 1753 Fragments arranged by size: 2461 1753 958 813 492 416 399 187 150 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7789 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2954 2954 Size: 7789 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2475 3210 6449 1239 Size: 1236 735 3239 2579 Fragments arranged by size: 3239 2579 1236 735 Bpu10I CC'TnA_GC Cuts at: 2053 2053 Size: 7789 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4410 5914 6779 7077 4410 Size: 1504 865 298 5122 Fragments arranged by size: 5122 1504 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6452 882 Size: 617 4953 2219 Fragments arranged by size: 4953 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5580 493 Size: 711 4376 2702 Fragments arranged by size: 4376 2702 711 BsaBI GATnn'nnATC Cuts at: 7783 7783 Size: 7789 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2935 5596 5978 6613 Size: 53 83 186 936 1402 2661 382 635 Cuts at: 6613 275 Size: 1451 Fragments arranged by size: 2661 1451 1402 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6222 6620 6894 7041 7452 6222 Size: 398 274 147 411 6559 Fragments arranged by size: 6559 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2779 2809 4784 4814 1178 Size: 30 165 30 1376 30 1975 30 4153 Fragments arranged by size: 4153 1975 1376 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2404 3101 4146 1186 Size: 1218 697 1045 4829 Fragments arranged by size: 4829 1218 1045 697 BseYI C'CCAG_C Cuts at: 2099 2626 4664 6992 7488 2099 Size: 527 2038 2328 496 2400 Fragments arranged by size: 2400 2328 2038 527 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3287 3374 1163 Size: 573 1551 87 5578 Fragments arranged by size: 5578 1551 573 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5239 6000 7028 1540 Size: 275 186 3238 761 1028 2301 Fragments arranged by size: 3238 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3207 5850 5935 7006 3207 Size: 2643 85 1071 3990 Fragments arranged by size: 3990 2643 1071 85 BsmI GAATG_Cn' Cuts at: 2897 3023 4747 5300 5393 2897 Size: 126 1724 553 93 5293 Fragments arranged by size: 5293 1724 553 126 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2942 2942 Size: 7789 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2544 2633 3115 3207 3322 3625 5108 Size: 399 754 89 482 92 115 303 1483 Cuts at: 5108 5850 5935 7006 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1483 1071 754 742 482 399 303 115 92 89 85 BspEI T'CCGG_A Cuts at: 7452 7452 Size: 7789 BspHI T'CATG_A Cuts at: 5678 7408 5678 Size: 1730 6059 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2870 3259 3446 5144 844 Size: 2026 389 187 1698 3489 Fragments arranged by size: 3489 2026 1698 389 187 BsrBI CCG'CTC Cuts at: 1394 1823 5676 1394 Size: 429 3853 3507 Fragments arranged by size: 3853 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2895 4504 6284 6458 65 Size: 1183 1647 1609 1780 174 1396 Fragments arranged by size: 1780 1647 1609 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4143 4658 6433 6620 1700 Size: 110 2333 515 1775 187 2869 Fragments arranged by size: 2869 2333 1775 515 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7789 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7789 BssSI C'ACGA_G Cuts at: 2910 5849 6861 2910 Size: 2939 1012 3838 Fragments arranged by size: 3838 2939 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7789 BstBI TT'CG_AA Cuts at: 6593 6593 Size: 7789 BstEII G'GTnAC_C Cuts at: 1184 4237 1184 Size: 3053 4736 BstYI r'GATC_y Cuts at: 1972 2475 3647 4168 4248 5006 5119 5486 5871 Size: 503 1172 521 80 758 113 367 385 Cuts at: 5871 5888 7340 7426 7438 7784 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1172 758 521 503 385 367 346 113 86 80 17 12 Bsu36I CC'TnA_GG Cuts at: 3954 3954 Size: 7789 BtgI C'CryG_G Cuts at: 513 1065 2015 2994 3924 4004 4788 513 Size: 552 950 979 930 80 784 3514 Fragments arranged by size: 3514 979 950 930 784 552 80 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7050 Fragments arranged by size: 7050 683 56 BtsI GCAGTG_nn' Cuts at: 5305 6099 6119 5305 Size: 794 20 6975 Fragments arranged by size: 6975 794 20 ClaI AT'CG_AT Cuts at: 1554 3963 1554 Size: 2409 5380 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3973 4008 529 Size: 35 3409 35 4310 Fragments arranged by size: 4310 3409 35 35 DraI TTT'AAA Cuts at: 2377 5093 2377 Size: 2716 5073 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6796 809 Size: 507 5480 1802 Fragments arranged by size: 5480 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5236 6125 7754 Size: 54 1425 50 275 18 3406 889 1629 Cuts at: 7754 8 Size: 43 Fragments arranged by size: 3406 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5236 1537 Size: 275 3424 4090 Fragments arranged by size: 4090 3424 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2718 3331 4086 4398 4607 5719 1353 Size: 1365 613 755 312 209 1112 3423 Fragments arranged by size: 3423 1365 1112 755 613 312 209 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4152 4214 6376 6750 6896 387 Size: 1064 138 2563 62 2162 374 146 1280 Fragments arranged by size: 2563 2162 1280 1064 374 146 138 62 EcoICRI GAG'CTC Cuts at: 3205 3205 Size: 7789 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2336 2475 3210 4110 4140 4200 4631 Size: 393 704 139 735 900 30 60 431 Cuts at: 4631 4863 4998 5852 6449 7236 7504 1239 Size: 232 135 854 597 787 268 1524 Fragments arranged by size: 1524 900 854 787 735 704 597 431 393 268 232 139 135 60 30 EcoNI CCTnn'n_nnAGG Cuts at: 3313 3313 Size: 7789 EcoO109I rG'GnC_Cy Cuts at: 2078 3621 3622 4606 2078 Size: 1543 1 984 5261 Fragments arranged by size: 5261 1543 984 1 EcoRI G'AATT_C Cuts at: 2905 5098 5476 2905 Size: 2193 378 5218 Fragments arranged by size: 5218 2193 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2366 3390 3528 4781 2366 Size: 1024 138 1253 5374 Fragments arranged by size: 5374 1253 1024 138 FspI TGC'GCA Cuts at: 6295 6295 Size: 7789 HaeII r_GCGC'y Cuts at: 1678 4665 4680 6936 1678 Size: 2987 15 2256 2531 Fragments arranged by size: 2987 2531 2256 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4541 4573 4784 4816 4825 Size: 32 135 32 2969 32 211 32 9 Cuts at: 4825 4857 6430 6462 6504 6536 1373 Size: 32 1573 32 42 32 2626 Fragments arranged by size: 2969 2626 1573 211 135 42 32 32 32 32 32 32 32 9 HincII GTy'rAC Cuts at: 669 1657 1938 5133 5379 669 Size: 988 281 3195 246 3079 Fragments arranged by size: 3195 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7789 HpaI GTT'AAC Cuts at: 5379 5379 Size: 7789 KpnI G_GTAC'C Cuts at: 5114 5114 Size: 7789 MfeI C'AATT_G Cuts at: 2328 5388 2328 Size: 3060 4729 MluI A'CGCG_T Cuts at: 7665 7665 Size: 7789 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2495 2660 2890 6903 7087 1305 Size: 1190 165 230 4013 184 2007 Fragments arranged by size: 4013 2007 1190 230 184 165 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5967 Fragments arranged by size: 5967 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2591 3440 3947 4757 5747 6106 6265 Size: 648 1425 849 507 810 990 359 159 Cuts at: 6265 518 Size: 2042 Fragments arranged by size: 2042 1425 990 849 810 648 507 359 159 MspA1I CmG'CkG Cuts at: 1606 2194 3586 3709 4883 5205 5882 7030 7275 Size: 588 1392 123 1174 322 677 1148 245 Cuts at: 7275 1606 Size: 2120 Fragments arranged by size: 2120 1392 1174 1148 677 588 322 245 123 NaeI GCC'GGC Cuts at: 4660 4660 Size: 7789 NcoI C'CATG_G Cuts at: 513 1065 2015 4004 4788 513 Size: 552 950 1989 784 3514 Fragments arranged by size: 3514 1989 950 784 552 NdeI CA'TA_TG Cuts at: 387 2467 4324 387 Size: 2080 1857 3852 Fragments arranged by size: 3852 2080 1857 NgoMIV G'CCGG_C Cuts at: 4658 4658 Size: 7789 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7789 NotI GC'GGCC_GC Cuts at: 5236 5236 Size: 7789 NsiI A_TGCA'T Cuts at: 2897 2897 Size: 7789 NspI r_CATG'y Cuts at: 1132 1229 2592 3154 5147 6692 1132 Size: 97 1363 562 1993 1545 2229 Fragments arranged by size: 2229 1993 1545 1363 562 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7789 PciI A'CATG_T Cuts at: 2588 6688 2588 Size: 4100 3689 PflMI CCAn_nnn'nTGG Cuts at: 1108 2453 4258 4485 1108 Size: 1345 1805 227 4412 Fragments arranged by size: 4412 1805 1345 227 PfoI T'CCnGG_A Cuts at: 3329 3329 Size: 7789 PmeI GTTT'AAAC Cuts at: 5093 5093 Size: 7789 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5858 5890 7371 7403 5858 Size: 32 1481 32 6244 Fragments arranged by size: 6244 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7789 PsiI TTA'TAA Cuts at: 2235 4347 5359 2235 Size: 2112 1012 4665 Fragments arranged by size: 4665 2112 1012 PspOMI G'GGCC_C Cuts at: 3621 3621 Size: 7789 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4050 4082 4050 Size: 32 7757 PstI C_TGCA'G Cuts at: 830 4399 5141 830 Size: 3569 742 3478 Fragments arranged by size: 3569 3478 742 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7789 PvuII CAG'CTG Cuts at: 2194 3709 4883 2194 Size: 1515 1174 5100 Fragments arranged by size: 5100 1515 1174 SacI G_AGCT'C Cuts at: 3207 3207 Size: 7789 SalI G'TCGA_C Cuts at: 1936 5131 1936 Size: 3195 4594 SapI GCTCTTCn'nnn_ Cuts at: 4398 4607 4398 Size: 209 7580 SbfI CC_TGCA'GG Cuts at: 5141 5141 Size: 7789 ScaI AGT'ACT Cuts at: 4860 5061 6037 7749 4860 Size: 201 976 1712 4900 Fragments arranged by size: 4900 1712 976 201 SexAI A'CCwGG_T Cuts at: 3605 3605 Size: 7789 SfcI C'TryA_G Cuts at: 826 1045 3987 4187 4395 4410 4828 5055 5137 Size: 219 2942 200 208 15 418 227 82 Cuts at: 5137 6272 6953 7144 826 Size: 1135 681 191 1471 Fragments arranged by size: 2942 1471 1135 681 418 227 219 208 200 191 82 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7789 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7789 SmaI CCC'GGG Cuts at: 1872 5116 1872 Size: 3244 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4425 5893 6794 7056 820 Size: 197 926 2482 1468 901 262 1553 Fragments arranged by size: 2482 1553 1468 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5580 493 Size: 5087 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7789 SphI G_CATG'C Cuts at: 1132 5147 1132 Size: 4015 3774 SspI AAT'ATT Cuts at: 5 52 5713 5 Size: 47 5661 2081 Fragments arranged by size: 5661 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7789 StyI C'CwwG_G Cuts at: 513 1065 2015 2758 3314 4004 4484 4788 5228 Size: 552 950 743 556 690 480 304 440 Cuts at: 5228 513 Size: 3074 Fragments arranged by size: 3074 950 743 690 556 552 480 440 304 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2660 3997 4328 5816 5833 5986 6171 Size: 1255 1080 1337 331 1488 17 153 185 Cuts at: 6171 6616 325 Size: 445 1498 Fragments arranged by size: 1498 1488 1337 1255 1080 445 331 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2393 4858 5059 Size: 274 80 33 51 1443 416 2465 201 Cuts at: 5059 6035 7747 96 Size: 976 1712 138 Fragments arranged by size: 2465 1712 1443 976 416 274 201 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2241 2273 2779 2811 2824 2856 4525 4557 4945 Size: 32 506 32 13 32 1669 32 388 Cuts at: 4945 4977 7749 7781 2241 Size: 32 2772 32 2249 Fragments arranged by size: 2772 2249 1669 506 388 32 32 32 32 32 32 13 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7789 XbaI T'CTAG_A Cuts at: 5125 5125 Size: 7789 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7789 XmaI C'CCGG_G Cuts at: 1870 5114 1870 Size: 3244 4545 XmnI GAAnn'nnTTC Cuts at: 2712 5918 2712 Size: 3206 4583 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5597 276 Size: 53 83 186 936 4063 2468 Fragments arranged by size: 4063 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspXI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI