(Circular) (Six-base) MAPSORT of: pFN21AA1492.seq Check: 783 from: 1 to: 7072 pFN21AA1492 7072 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4882 278 Size: 53 83 186 936 3346 2468 Fragments arranged by size: 3346 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2441 4242 4415 1937 Size: 504 1801 173 4594 Fragments arranged by size: 4594 1801 504 173 Acc65I G'GTAC_C Cuts at: 2764 4393 2764 Size: 1629 5443 AclI AA'CG_TT Cuts at: 1563 5199 5572 1563 Size: 3636 373 3063 Fragments arranged by size: 3636 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3375 3566 3581 4297 5135 6519 6787 1632 Size: 1743 191 15 716 838 1384 268 1917 Fragments arranged by size: 1917 1743 1384 838 716 268 191 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6875 AflIII A'CryG_T Cuts at: 1203 2287 5971 6948 1203 Size: 1084 3684 977 1327 Fragments arranged by size: 3684 1327 1084 977 AgeI A'CCGG_T Cuts at: 5903 5903 Size: 7072 AhdI GACnn_n'nnGTC Cuts at: 5801 5801 Size: 7072 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7072 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2630 3557 1184 Size: 1446 927 4699 Fragments arranged by size: 4699 1446 927 AlwNI CAG_nnn'CTG Cuts at: 2557 3149 3808 4815 6387 2557 Size: 592 659 1007 1572 3242 Fragments arranged by size: 3242 1572 1007 659 592 ApaLI G'TGCA_C Cuts at: 5129 6285 5129 Size: 1156 5916 ApoI r'AATT_y Cuts at: 1493 2170 3131 3173 3294 3813 3878 4307 4381 Size: 677 961 42 121 519 65 429 74 Cuts at: 4381 4607 4759 6868 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 961 677 519 429 226 152 121 74 65 42 AseI AT'TA_AT Cuts at: 160 1032 4142 4196 5626 160 Size: 872 3110 54 1430 1606 Fragments arranged by size: 3110 1606 1430 872 54 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7072 AvaI C'yCGr_G Cuts at: 1870 1943 4397 1870 Size: 73 2454 4545 Fragments arranged by size: 4545 2454 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2247 2280 2247 Size: 33 7039 BamHI G'GATC_C Cuts at: 3995 4402 4769 3995 Size: 407 367 6298 Fragments arranged by size: 6298 407 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2764 4393 5848 618 Size: 325 217 627 977 1629 1455 1842 Fragments arranged by size: 1842 1629 1455 977 627 325 217 BanII G_rGCy'C Cuts at: 1391 2907 3108 4391 1391 Size: 1516 201 1283 4072 Fragments arranged by size: 4072 1516 1283 201 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2205 928 Size: 971 306 5795 Fragments arranged by size: 5795 971 306 BbvCI CC'TCA_GC Cuts at: 4036 4036 Size: 7072 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5263 5297 1903 Size: 34 50 34 3242 34 3678 Fragments arranged by size: 3678 3242 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2814 2832 2838 4964 6174 2814 Size: 18 6 2126 1210 3712 Fragments arranged by size: 3712 2126 1210 18 6 BclI T'GATC_A Cuts at: 1548 1548 Size: 7072 BfrBI ATG'CAT Cuts at: 3378 3378 Size: 7072 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5683 136 Size: 107 122 71 631 605 4011 1525 Fragments arranged by size: 4011 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3264 7067 3264 Size: 3803 3269 BlpI GC'TnA_GC Cuts at: 4489 4489 Size: 7072 Bme1580I G_kGCm'C Cuts at: 1790 5133 6289 1790 Size: 3343 1156 2573 Fragments arranged by size: 3343 2573 1156 BmgBI CAC'GTC Cuts at: 1318 3167 1318 Size: 1849 5223 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2057 3259 3473 Size: 416 53 83 399 24 640 1202 214 Cuts at: 3473 3742 4803 5761 442 Size: 269 1061 958 1753 Fragments arranged by size: 1753 1202 1061 958 640 416 399 269 214 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7072 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2484 2814 4239 5732 1239 Size: 1245 330 1425 1493 2579 Fragments arranged by size: 2579 1493 1425 1245 330 Bpu10I CC'TnA_GC Cuts at: 4036 4036 Size: 7072 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5197 6062 6360 5197 Size: 865 298 5909 Fragments arranged by size: 5909 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3026 5735 882 Size: 617 1527 2709 2219 Fragments arranged by size: 2709 2219 1527 617 BsaAI yAC'GTr Cuts at: 493 1204 3081 4863 493 Size: 711 1877 1782 2702 Fragments arranged by size: 2702 1877 1782 711 BsaBI GATnn'nnATC Cuts at: 3263 7066 3263 Size: 3803 3269 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4879 5261 5896 275 Size: 53 83 186 936 3346 382 635 1451 Fragments arranged by size: 3346 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5505 5903 6177 6324 6735 5505 Size: 398 274 147 411 5842 Fragments arranged by size: 5842 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3095 3125 3890 3920 1178 Size: 30 165 30 1692 30 765 30 4330 Fragments arranged by size: 4330 1692 765 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3116 1186 Size: 1930 5142 BseYI C'CCAG_C Cuts at: 3508 6275 6771 3508 Size: 2767 496 3809 Fragments arranged by size: 3809 2767 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6499 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4522 5283 6311 1540 Size: 275 186 2521 761 1028 2301 Fragments arranged by size: 2521 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2907 3311 5133 5218 6289 2907 Size: 404 1822 85 1071 3690 Fragments arranged by size: 3690 1822 1071 404 85 BsmI GAATG_Cn' Cuts at: 2692 4583 4676 2692 Size: 1891 93 5088 Fragments arranged by size: 5088 1891 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3114 3114 Size: 7072 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2907 3108 3311 4391 5133 5218 6289 Size: 399 1117 201 203 1080 742 85 1071 Cuts at: 6289 1391 Size: 2174 Fragments arranged by size: 2174 1117 1080 1071 742 399 203 201 85 BspEI T'CCGG_A Cuts at: 6735 6735 Size: 7072 BspHI T'CATG_A Cuts at: 3393 4961 6691 3393 Size: 1568 1730 3774 Fragments arranged by size: 3774 1730 1568 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2828 4228 4427 844 Size: 1984 1400 199 3489 Fragments arranged by size: 3489 1984 1400 199 BsrBI CCG'CTC Cuts at: 1394 1823 4959 1394 Size: 429 3136 3507 Fragments arranged by size: 3507 3136 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2612 5567 5741 65 Size: 1183 1364 2955 174 1396 Fragments arranged by size: 2955 1396 1364 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5716 5903 1700 Size: 110 3906 187 2869 Fragments arranged by size: 3906 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3339 3407 96 Size: 3243 68 3761 Fragments arranged by size: 3761 3243 68 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7072 BssSI C'ACGA_G Cuts at: 5132 6144 5132 Size: 1012 6060 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7072 BstBI TT'CG_AA Cuts at: 5876 5876 Size: 7072 BstEII G'GTnAC_C Cuts at: 1184 2715 1184 Size: 1531 5541 BstXI CCAn_nnnn'nTGG Cuts at: 2980 2980 Size: 7072 BstYI r'GATC_y Cuts at: 1972 3264 3995 4402 4769 5154 5171 6623 6709 Size: 1292 731 407 367 385 17 1452 86 Cuts at: 6709 6721 7067 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1292 731 407 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2442 2442 Size: 7072 Bsu36I CC'TnA_GG Cuts at: 2832 2832 Size: 7072 BtgI C'CryG_G Cuts at: 513 1065 2023 2772 3999 513 Size: 552 958 749 1227 3586 Fragments arranged by size: 3586 1227 958 749 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2686 487 Size: 683 56 1460 4873 Fragments arranged by size: 4873 1460 683 56 BtsI GCAGTG_nn' Cuts at: 4588 5382 5402 4588 Size: 794 20 6258 Fragments arranged by size: 6258 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7072 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7037 DraI TTT'AAA Cuts at: 2391 2926 3988 4376 2391 Size: 535 1062 388 5087 Fragments arranged by size: 5087 1062 535 388 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6079 809 Size: 507 4763 1802 Fragments arranged by size: 4763 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4519 5408 7037 Size: 54 1425 50 275 18 2689 889 1629 Cuts at: 7037 8 Size: 43 Fragments arranged by size: 2689 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4519 1537 Size: 275 2707 4090 Fragments arranged by size: 4090 2707 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2673 3675 4268 5002 1353 Size: 1320 1002 593 734 3423 Fragments arranged by size: 3423 1320 1002 734 593 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5659 6033 6179 387 Size: 1064 138 4070 374 146 1280 Fragments arranged by size: 4070 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2905 2905 Size: 7072 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2484 2814 3375 3566 3581 4239 4297 Size: 393 852 330 561 191 15 658 58 Cuts at: 4297 5135 5732 6519 6787 1239 Size: 838 597 787 268 1524 Fragments arranged by size: 1524 852 838 787 658 597 561 393 330 268 191 58 15 EcoNI CCTnn'n_nnAGG Cuts at: 2835 2835 Size: 7072 EcoO109I rG'GnC_Cy Cuts at: 3475 3475 Size: 7072 EcoRI G'AATT_C Cuts at: 4381 4759 4381 Size: 378 6694 FspI TGC'GCA Cuts at: 2009 5578 2009 Size: 3569 3503 HaeII r_GCGC'y Cuts at: 1678 6219 1678 Size: 4541 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3056 3088 4267 4299 5713 Size: 32 135 32 1484 32 1179 32 1414 Cuts at: 5713 5745 5787 5819 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1484 1414 1179 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4416 4662 669 Size: 988 281 2478 246 3079 Fragments arranged by size: 3079 2478 988 281 246 HindIII A'AGCT_T Cuts at: 748 3980 748 Size: 3232 3840 HpaI GTT'AAC Cuts at: 4662 4662 Size: 7072 KpnI G_GTAC'C Cuts at: 2768 4397 2768 Size: 1629 5443 MfeI C'AATT_G Cuts at: 4671 4671 Size: 7072 MluI A'CGCG_T Cuts at: 6948 6948 Size: 7072 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4204 6186 6370 1305 Size: 2899 1982 184 2007 Fragments arranged by size: 2899 2007 1982 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5250 Fragments arranged by size: 5250 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2132 3188 3752 5030 5389 5548 518 Size: 648 966 1056 564 1278 359 159 2042 Fragments arranged by size: 2042 1278 1056 966 648 564 359 159 MspA1I CmG'CkG Cuts at: 1606 2025 2557 3337 3706 3872 4488 5165 6313 Size: 419 532 780 369 166 616 677 1148 Cuts at: 6313 6558 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 780 677 616 532 419 369 245 166 NcoI C'CATG_G Cuts at: 513 1065 2772 513 Size: 552 1707 4813 Fragments arranged by size: 4813 1707 552 NdeI CA'TA_TG Cuts at: 387 2410 2452 387 Size: 2023 42 5007 Fragments arranged by size: 5007 2023 42 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7072 NotI GC'GGCC_GC Cuts at: 4519 4519 Size: 7072 NsiI A_TGCA'T Cuts at: 3380 3380 Size: 7072 NspI r_CATG'y Cuts at: 1132 1229 2291 4430 5975 1132 Size: 97 1062 2139 1545 2229 Fragments arranged by size: 2229 2139 1545 1062 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7072 PciI A'CATG_T Cuts at: 2287 5971 2287 Size: 3684 3388 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7072 PmeI GTTT'AAAC Cuts at: 4376 4376 Size: 7072 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5141 5173 6654 6686 5141 Size: 32 1481 32 5527 Fragments arranged by size: 5527 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3475 3475 Size: 7072 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7072 PsiI TTA'TAA Cuts at: 4642 4642 Size: 7072 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3606 3638 3606 Size: 32 7040 PstI C_TGCA'G Cuts at: 830 4424 830 Size: 3594 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7072 PvuII CAG'CTG Cuts at: 2557 3337 3706 3872 2557 Size: 780 369 166 5757 Fragments arranged by size: 5757 780 369 166 SacI G_AGCT'C Cuts at: 2907 2907 Size: 7072 SacII CC_GC'GG Cuts at: 2026 2026 Size: 7072 SalI G'TCGA_C Cuts at: 1936 4414 1936 Size: 2478 4594 SanDI GG'GwC_CC Cuts at: 3475 3475 Size: 7072 SapI GCTCTTCn'nnn_ Cuts at: 3675 4268 3675 Size: 593 6479 SbfI CC_TGCA'GG Cuts at: 4424 4424 Size: 7072 ScaI AGT'ACT Cuts at: 5320 7032 5320 Size: 1712 5360 SfcI C'TryA_G Cuts at: 826 1045 3039 4291 4420 5555 6236 6427 826 Size: 219 1994 1252 129 1135 681 191 1471 Fragments arranged by size: 1994 1471 1252 1135 681 219 191 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7072 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7072 SmaI CCC'GGG Cuts at: 1872 4399 1872 Size: 2527 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5176 6077 6339 820 Size: 197 926 3233 901 262 1553 Fragments arranged by size: 3233 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4863 493 Size: 4370 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7072 SphI G_CATG'C Cuts at: 1132 4430 1132 Size: 3298 3774 SspI AAT'ATT Cuts at: 5 52 3518 4996 5 Size: 47 3466 1478 2081 Fragments arranged by size: 3466 2081 1478 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7072 StyI C'CwwG_G Cuts at: 513 1065 2545 2772 3470 4057 4511 513 Size: 552 1480 227 698 587 454 3074 Fragments arranged by size: 3074 1480 698 587 552 454 227 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3669 5099 5116 5269 5454 5899 325 Size: 1255 2089 1430 17 153 185 445 1498 Fragments arranged by size: 2089 1498 1430 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3339 3407 5318 Size: 274 80 33 51 1443 1362 68 1911 Cuts at: 5318 7030 96 Size: 1712 138 Fragments arranged by size: 1911 1712 1443 1362 274 138 80 68 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7032 7064 7032 Size: 32 7040 Tth111I GACn'n_nGTC Cuts at: 1316 2516 1316 Size: 1200 5872 XbaI T'CTAG_A Cuts at: 3116 4408 3116 Size: 1292 5780 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2073 2191 2241 2329 3646 2011 Size: 62 118 50 88 1317 5437 Fragments arranged by size: 5437 1317 118 88 62 50 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7072 XmaI C'CCGG_G Cuts at: 1870 4397 1870 Size: 2527 4545 XmnI GAAnn'nnTTC Cuts at: 5201 5201 Size: 7072 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4880 276 Size: 53 83 186 936 3346 2468 Fragments arranged by size: 3346 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SexAI SfiI SfoI SrfI SwaI