Restriction map for
pFN21AA1492
(Linear) (Six-base) MAPSORT of: KIAA1492.seq Check: 3941 from: 1 to: 2367
KIAA1492.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AccI GT'mk_AC
Cuts at: 0 436 2237 2367
Size: 436 1801 130
Fragments arranged by size:
1801 436 130
Acc65I G'GTAC_C
Cuts at: 0 759 2367
Size: 759 1608
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1370 1561 1576 2292 2367
Size: 1370 191 15 716 75
Fragments arranged by size:
1370 716 191 75 15
AflIII A'CryG_T
Cuts at: 0 282 2367
Size: 282 2085
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 625 1552 2367
Size: 625 927 815
Fragments arranged by size:
927 815 625
AlwNI CAG_nnn'CTG
Cuts at: 0 552 1144 1803 2367
Size: 552 592 659 564
Fragments arranged by size:
659 592 564 552
ApoI r'AATT_y
Cuts at: 0 165 1126 1168 1289 1808 1873 2302 2367
Size: 165 961 42 121 519 65 429 65
Fragments arranged by size:
961 519 429 165 121 65 65 42
AseI AT'TA_AT
Cuts at: 0 2137 2191 2367
Size: 2137 54 176
Fragments arranged by size:
2137 176 54
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 242 275 2367
Size: 242 33 2092
Fragments arranged by size:
2092 242 33
BamHI G'GATC_C
Cuts at: 0 1990 2367
Size: 1990 377
BanI G'GyrC_C
Cuts at: 0 759 2367
Size: 759 1608
BanII G_rGCy'C
Cuts at: 0 902 1103 2367
Size: 902 201 1264
Fragments arranged by size:
1264 902 201
BbsI GAAGACnn'nnnn_
Cuts at: 0 200 2367
Size: 200 2167
BbvCI CC'TCA_GC
Cuts at: 0 2031 2367
Size: 2031 336
BciVI GTATCCnnnnn_n'
Cuts at: 0 809 827 833 2367
Size: 809 18 6 1534
Fragments arranged by size:
1534 809 18 6
BfrBI ATG'CAT
Cuts at: 0 1373 2367
Size: 1373 994
BglII A'GATC_T
Cuts at: 0 1259 2367
Size: 1259 1108
BmgBI CAC'GTC
Cuts at: 0 1162 2367
Size: 1162 1205
BmrI ACTGGGnnnn_n'
Cuts at: 0 52 1254 1468 1737 2367
Size: 52 1202 214 269 630
Fragments arranged by size:
1202 630 269 214 52
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 809 2234 2367
Size: 479 330 1425 133
Fragments arranged by size:
1425 479 330 133
Bpu10I CC'TnA_GC
Cuts at: 0 2031 2367
Size: 2031 336
BsaI GGTCTCn'nnnn_
Cuts at: 0 1021 2367
Size: 1021 1346
BsaAI yAC'GTr
Cuts at: 0 1076 2367
Size: 1076 1291
BsaBI GATnn'nnATC
Cuts at: 0 1258 2367
Size: 1258 1109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1090 1120 1885 1915 2367
Size: 1090 30 765 30 452
Fragments arranged by size:
1090 765 452 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1111 2367
Size: 1111 1256
BseYI C'CCAG_C
Cuts at: 0 1503 2367
Size: 1503 864
BsiHKAI G_wGCw'C
Cuts at: 0 902 1306 2367
Size: 902 404 1061
Fragments arranged by size:
1061 902 404
BsmI GAATG_Cn'
Cuts at: 0 687 2367
Size: 687 1680
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1109 2367
Size: 1109 1258
Bsp1286I G_dGCh'C
Cuts at: 0 902 1103 1306 2367
Size: 902 201 203 1061
Fragments arranged by size:
1061 902 203 201
BspHI T'CATG_A
Cuts at: 0 1388 2367
Size: 1388 979
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 823 2223 2367
Size: 823 1400 144
Fragments arranged by size:
1400 823 144
BsrDI GCAATG_nn'
Cuts at: 0 607 2367
Size: 607 1760
BsrGI T'GTAC_A
Cuts at: 0 1334 1402 2367
Size: 1334 68 965
Fragments arranged by size:
1334 965 68
BstEII G'GTnAC_C
Cuts at: 0 710 2367
Size: 710 1657
BstXI CCAn_nnnn'nTGG
Cuts at: 0 975 2367
Size: 975 1392
BstYI r'GATC_y
Cuts at: 0 1259 1990 2367
Size: 1259 731 377
Fragments arranged by size:
1259 731 377
BstZ17I GTA'TAC
Cuts at: 0 437 2367
Size: 437 1930
Bsu36I CC'TnA_GG
Cuts at: 0 827 2367
Size: 827 1540
BtgI C'CryG_G
Cuts at: 0 18 767 1994 2367
Size: 18 749 1227 373
Fragments arranged by size:
1227 749 373 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 681 2367
Size: 681 1686
DraI TTT'AAA
Cuts at: 0 386 921 1983 2367
Size: 386 535 1062 384
Fragments arranged by size:
1062 535 386 384
EarI CTCTTCn'nnn_
Cuts at: 0 668 1670 2263 2367
Size: 668 1002 593 104
Fragments arranged by size:
1002 668 593 104
EcoICRI GAG'CTC
Cuts at: 0 900 2367
Size: 900 1467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 809 1370 1561 1576 2234 2292 2367
Size: 479 330 561 191 15 658 58 75
Fragments arranged by size:
658 561 479 330 191 75 58 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 830 2367
Size: 830 1537
EcoO109I rG'GnC_Cy
Cuts at: 0 1470 2367
Size: 1470 897
FspI TGC'GCA
Cuts at: 0 4 2367
Size: 4 2363
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 2262 2294 2367
Size: 1051 32 1179 32 73
Fragments arranged by size:
1179 1051 73 32 32
HindIII A'AGCT_T
Cuts at: 0 1975 2367
Size: 1975 392
KpnI G_GTAC'C
Cuts at: 0 763 2367
Size: 763 1604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2199 2367
Size: 2199 168
MslI CAynn'nnrTG
Cuts at: 0 127 1183 1747 2367
Size: 127 1056 564 620
Fragments arranged by size:
1056 620 564 127
MspA1I CmG'CkG
Cuts at: 0 20 552 1332 1701 1867 2367
Size: 20 532 780 369 166 500
Fragments arranged by size:
780 532 500 369 166 20
NcoI C'CATG_G
Cuts at: 0 767 2367
Size: 767 1600
NdeI CA'TA_TG
Cuts at: 0 405 447 2367
Size: 405 42 1920
Fragments arranged by size:
1920 405 42
NsiI A_TGCA'T
Cuts at: 0 1375 2367
Size: 1375 992
NspI r_CATG'y
Cuts at: 0 286 2367
Size: 286 2081
PciI A'CATG_T
Cuts at: 0 282 2367
Size: 282 2085
PpuMI rG'GwC_Cy
Cuts at: 0 1470 2367
Size: 1470 897
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1601 1633 2367
Size: 1601 32 734
Fragments arranged by size:
1601 734 32
PvuII CAG'CTG
Cuts at: 0 552 1332 1701 1867 2367
Size: 552 780 369 166 500
Fragments arranged by size:
780 552 500 369 166
SacI G_AGCT'C
Cuts at: 0 902 2367
Size: 902 1465
SacII CC_GC'GG
Cuts at: 0 21 2367
Size: 21 2346
SanDI GG'GwC_CC
Cuts at: 0 1470 2367
Size: 1470 897
SapI GCTCTTCn'nnn_
Cuts at: 0 1670 2263 2367
Size: 1670 593 104
Fragments arranged by size:
1670 593 104
SfcI C'TryA_G
Cuts at: 0 1034 2286 2367
Size: 1034 1252 81
Fragments arranged by size:
1252 1034 81
SspI AAT'ATT
Cuts at: 0 1513 2367
Size: 1513 854
StyI C'CwwG_G
Cuts at: 0 540 767 1465 2052 2367
Size: 540 227 698 587 315
Fragments arranged by size:
698 587 540 315 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1664 2367
Size: 1664 703
TatI w'GTAC_w
Cuts at: 0 1334 1402 2367
Size: 1334 68 965
Fragments arranged by size:
1334 965 68
Tth111I GACn'n_nGTC
Cuts at: 0 511 2367
Size: 511 1856
XbaI T'CTAG_A
Cuts at: 0 1111 2367
Size: 1111 1256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 68 186 236 324 1641 2367
Size: 68 118 50 88 1317 726
Fragments arranged by size:
1317 726 118 88 68 50
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlfI AlwNI ApoI AseI
BaeI BamHI BanI BanII BbsI BbvCI BciVI BfrBI
BglII BmgBI BmrI BpmI Bpu10I BsaI BsaAI BsaBI
BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrGI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI DraI EarI EcoICRI Eco57MI EcoNI EcoO109I
FspI Hin4I HindIII KpnI MmeI MslI MspA1I NcoI
NdeI NsiI NspI PciI PpuMI PsrI PvuII SacI
SacII SanDI SapI SfcI SspI StyI TaqII TatI
Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BbeI
BcgI BclI BglI BlpI Bme1580I BmtI BplI BpuEI
BsaHI BsaWI BsgI BsiEI BsiWI BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BtsI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoRI EcoRV FalI FseI
FspAI HaeII HincII HpaI KasI MfeI MluI MscI
NaeI NarI NgoMIV NheI NotI NruI PacI PasI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI RsrII SalI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI SwaI TstI XhoI XmaI XmnI
ZraI