(Circular) (Six-base) MAPSORT of: pFN21AA1480.seq Check: 5599 from: 1 to: 7129 pFN21AA1480 7129 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3919 3919 Size: 7129 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4939 278 Size: 53 83 186 936 3403 2468 Fragments arranged by size: 3403 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2496 3257 4472 1937 Size: 559 761 1215 4594 Fragments arranged by size: 4594 1215 761 559 Acc65I G'GTAC_C Cuts at: 4450 4450 Size: 7129 AclI AA'CG_TT Cuts at: 1563 2251 4050 5256 5629 1563 Size: 688 1799 1206 373 3063 Fragments arranged by size: 3063 1799 1206 688 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5192 6576 6844 1632 Size: 3560 1384 268 1917 Fragments arranged by size: 3560 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6932 AflIII A'CryG_T Cuts at: 1203 3792 6028 7005 1203 Size: 2589 2236 977 1327 Fragments arranged by size: 2589 2236 1327 977 AgeI A'CCGG_T Cuts at: 5960 5960 Size: 7129 AhdI GACnn_n'nnGTC Cuts at: 5858 5858 Size: 7129 AleI CACnn'nnGTG Cuts at: 1166 2342 3743 3821 4206 1166 Size: 1176 1401 78 385 4089 Fragments arranged by size: 4089 1401 1176 385 78 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2967 1184 Size: 1783 5346 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3605 3637 3605 Size: 32 7097 AlwNI CAG_nnn'CTG Cuts at: 4872 6444 4872 Size: 1572 5557 ApaI G_GGCC'C Cuts at: 4141 4193 4141 Size: 52 7077 ApaLI G'TGCA_C Cuts at: 5186 6342 5186 Size: 1156 5973 ApoI r'AATT_y Cuts at: 1493 4438 4664 4816 6925 1493 Size: 2945 226 152 2109 1697 Fragments arranged by size: 2945 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5683 160 Size: 872 4651 1606 Fragments arranged by size: 4651 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 AvaI C'yCGr_G Cuts at: 1870 1943 2166 2743 3678 4422 4454 1870 Size: 73 223 577 935 744 32 4545 Fragments arranged by size: 4545 935 744 577 223 73 32 BamHI G'GATC_C Cuts at: 3589 4459 4826 3589 Size: 870 367 5892 Fragments arranged by size: 5892 870 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2162 2218 3766 4092 4450 Size: 325 217 627 375 56 1548 326 358 Cuts at: 4450 5905 618 Size: 1455 1842 Fragments arranged by size: 1842 1548 1455 627 375 358 326 325 217 56 BanII G_rGCy'C Cuts at: 1391 2045 2415 2658 3342 4104 4141 4193 4448 Size: 654 370 243 684 762 37 52 255 Cuts at: 4448 1391 Size: 4072 Fragments arranged by size: 4072 762 684 654 370 255 243 52 37 BbeI G_GCGC'C Cuts at: 4096 4096 Size: 7129 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2427 2930 928 Size: 971 528 503 5127 Fragments arranged by size: 5127 971 528 503 BbvCI CC'TCA_GC Cuts at: 2102 4122 2102 Size: 2020 5109 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2536 2557 2570 2591 2681 2715 3347 Size: 34 599 21 13 21 90 34 632 Cuts at: 3347 3381 5320 5354 1903 Size: 34 1939 34 3678 Fragments arranged by size: 3678 1939 632 599 90 34 34 34 34 21 21 13 BciVI GTATCCnnnnn_n' Cuts at: 3829 5021 6231 3829 Size: 1192 1210 4727 Fragments arranged by size: 4727 1210 1192 BclI T'GATC_A Cuts at: 1548 1548 Size: 7129 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4028 4108 5740 Size: 107 122 71 631 605 2356 80 1632 Cuts at: 5740 136 Size: 1525 Fragments arranged by size: 2356 1632 1525 631 605 122 107 80 71 BglII A'GATC_T Cuts at: 7124 7124 Size: 7129 BlpI GC'TnA_GC Cuts at: 4546 4546 Size: 7129 Bme1580I G_kGCm'C Cuts at: 1790 2167 2223 2324 2349 3771 4141 4193 5190 Size: 377 56 101 25 1422 370 52 997 Cuts at: 5190 6346 1790 Size: 1156 2573 Fragments arranged by size: 2573 1422 1156 997 377 370 101 56 52 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7129 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2175 2309 3274 Size: 416 53 83 399 24 758 134 965 Cuts at: 3274 3594 4196 4860 5818 442 Size: 320 602 664 958 1753 Fragments arranged by size: 1753 965 958 758 664 602 416 399 320 134 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7129 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3704 3704 Size: 7129 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2646 3904 4143 5789 1239 Size: 1407 1258 239 1646 2579 Fragments arranged by size: 2579 1646 1407 1258 239 Bpu10I CC'TnA_GC Cuts at: 2039 2102 2156 4122 2039 Size: 63 54 1966 5046 Fragments arranged by size: 5046 1966 63 54 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3101 5254 6119 6417 3101 Size: 2153 865 298 3813 Fragments arranged by size: 3813 2153 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2709 3119 3233 3281 3675 5792 882 Size: 617 1210 410 114 48 394 2117 2219 Fragments arranged by size: 2219 2117 1210 617 410 394 114 48 BsaAI yAC'GTr Cuts at: 493 1204 2998 4920 493 Size: 711 1794 1922 2702 Fragments arranged by size: 2702 1922 1794 711 BsaBI GATnn'nnATC Cuts at: 7123 7123 Size: 7129 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4093 4936 5318 5953 Size: 53 83 186 936 2560 843 382 635 Cuts at: 5953 275 Size: 1451 Fragments arranged by size: 2560 1451 936 843 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2292 3832 5562 5960 6234 6381 6792 2292 Size: 1540 1730 398 274 147 411 2629 Fragments arranged by size: 2629 1730 1540 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4397 4427 1178 Size: 30 165 30 2994 30 3880 Fragments arranged by size: 3880 2994 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2762 4178 4181 1186 Size: 1576 1416 3 4134 Fragments arranged by size: 4134 1576 1416 3 BseYI C'CCAG_C Cuts at: 2122 2682 2844 6332 6828 2122 Size: 560 162 3488 496 2423 Fragments arranged by size: 3488 2423 560 496 162 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6556 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2241 4579 5340 6368 1540 Size: 275 186 240 2338 761 1028 2301 Fragments arranged by size: 2338 2301 1028 761 275 240 186 BsiHKAI G_wGCw'C Cuts at: 3890 5190 5275 6346 3890 Size: 1300 85 1071 4673 Fragments arranged by size: 4673 1300 1071 85 BsmI GAATG_Cn' Cuts at: 3934 4640 4733 3934 Size: 706 93 6330 Fragments arranged by size: 6330 706 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3187 3187 Size: 7129 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2167 2223 2324 2349 2415 2658 Size: 399 255 122 56 101 25 66 243 Cuts at: 2658 3342 3771 3890 4104 4141 4193 4448 5190 Size: 684 429 119 214 37 52 255 742 Cuts at: 5190 5275 6346 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 684 429 399 255 255 243 214 122 119 101 85 66 56 52 37 25 BspEI T'CCGG_A Cuts at: 2292 3832 6792 2292 Size: 1540 2960 2629 Fragments arranged by size: 2960 2629 1540 BspHI T'CATG_A Cuts at: 3423 3555 4299 5018 6748 3423 Size: 132 744 719 1730 3804 Fragments arranged by size: 3804 1730 744 719 132 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3792 3919 4484 844 Size: 2948 127 565 3489 Fragments arranged by size: 3489 2948 565 127 BsrBI CCG'CTC Cuts at: 1394 1823 5016 1394 Size: 429 3193 3507 Fragments arranged by size: 3507 3193 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2575 4247 4352 5624 5798 65 Size: 1183 1327 1672 105 1272 174 1396 Fragments arranged by size: 1672 1396 1327 1272 1183 174 105 BsrFI r'CCGG_y Cuts at: 1700 1810 2362 3601 4214 5773 5960 1700 Size: 110 552 1239 613 1559 187 2869 Fragments arranged by size: 2869 1559 1239 613 552 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7129 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7129 BssSI C'ACGA_G Cuts at: 3987 4202 5189 6201 3987 Size: 215 987 1012 4915 Fragments arranged by size: 4915 1012 987 215 BstAPI GCAn_nnn'nTGC Cuts at: 794 4354 794 Size: 3560 3569 BstBI TT'CG_AA Cuts at: 5933 5933 Size: 7129 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7129 BstXI CCAn_nnnn'nTGG Cuts at: 2884 2962 3193 3745 4024 4336 2884 Size: 78 231 552 279 312 5677 Fragments arranged by size: 5677 552 312 279 231 78 BstYI r'GATC_y Cuts at: 1972 2187 2667 3048 3589 4459 4826 5211 5228 Size: 215 480 381 541 870 367 385 17 Cuts at: 5228 6680 6766 6778 7124 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 870 541 480 385 381 367 346 215 86 17 12 BstZ17I GTA'TAC Cuts at: 3258 3258 Size: 7129 Bsu36I CC'TnA_GG Cuts at: 3144 3216 3144 Size: 72 7057 BtgI C'CryG_G Cuts at: 513 1065 2052 2503 2919 4023 513 Size: 552 987 451 416 1104 3619 Fragments arranged by size: 3619 1104 987 552 451 416 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4003 487 Size: 683 56 2777 3613 Fragments arranged by size: 3613 2777 683 56 BtsI GCAGTG_nn' Cuts at: 2174 2373 3409 4242 4645 5439 5459 2174 Size: 199 1036 833 403 794 20 3844 Fragments arranged by size: 3844 1036 833 794 403 199 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7129 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7094 DraI TTT'AAA Cuts at: 4433 4433 Size: 7129 DraIII CAC_nnn'GTG Cuts at: 2082 2082 Size: 7129 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3179 6136 809 Size: 507 1863 2957 1802 Fragments arranged by size: 2957 1863 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3896 4576 5465 Size: 54 1425 50 275 18 2066 680 889 Cuts at: 5465 7094 8 Size: 1629 43 Fragments arranged by size: 2066 1629 1425 889 680 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4576 1537 Size: 275 2764 4090 Fragments arranged by size: 4090 2764 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3320 5059 1353 Size: 1967 1739 3423 Fragments arranged by size: 3423 1967 1739 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5716 6090 6236 387 Size: 1064 138 4127 374 146 1280 Fragments arranged by size: 4127 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2646 3904 4143 5192 5789 6576 6844 Size: 393 1014 1258 239 1049 597 787 268 Cuts at: 6844 1239 Size: 1524 Fragments arranged by size: 1524 1258 1049 1014 787 597 393 268 239 EcoNI CCTnn'n_nnAGG Cuts at: 3489 3489 Size: 7129 EcoO109I rG'GnC_Cy Cuts at: 2119 2195 2674 3494 3939 4029 4137 4138 4190 Size: 76 479 820 445 90 108 1 52 Cuts at: 4190 2119 Size: 5058 Fragments arranged by size: 5058 820 479 445 108 90 76 52 1 EcoRI G'AATT_C Cuts at: 4438 4816 4438 Size: 378 6751 EcoRV GAT'ATC Cuts at: 2389 2389 Size: 7129 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3766 3766 Size: 7129 FspI TGC'GCA Cuts at: 5635 5635 Size: 7129 HaeII r_GCGC'y Cuts at: 1678 2102 4096 4279 6276 1678 Size: 424 1994 183 1997 2531 Fragments arranged by size: 2531 1997 1994 424 183 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2802 2834 3140 3172 3228 Size: 32 135 32 1230 32 306 32 56 Cuts at: 3228 3260 3343 3375 3574 3606 5770 5802 5844 Size: 32 83 32 199 32 2164 32 42 Cuts at: 5844 5876 1373 Size: 32 2626 Fragments arranged by size: 2626 2164 1230 306 199 135 83 56 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2137 3100 4473 4719 669 Size: 988 281 199 963 1373 246 3079 Fragments arranged by size: 3079 1373 988 963 281 246 199 HindIII A'AGCT_T Cuts at: 748 748 Size: 7129 HpaI GTT'AAC Cuts at: 4719 4719 Size: 7129 KasI G'GCGC_C Cuts at: 4092 4092 Size: 7129 KpnI G_GTAC'C Cuts at: 4454 4454 Size: 7129 MfeI C'AATT_G Cuts at: 4728 4728 Size: 7129 MluI A'CGCG_T Cuts at: 7005 7005 Size: 7129 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2581 6243 6427 1305 Size: 1276 3662 184 2007 Fragments arranged by size: 3662 2007 1276 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5307 Fragments arranged by size: 5307 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2342 3456 3743 3821 4022 4206 4319 Size: 648 1176 1114 287 78 201 184 113 Cuts at: 4319 5087 5446 5605 518 Size: 768 359 159 2042 Fragments arranged by size: 2042 1176 1114 768 648 359 287 201 184 159 113 78 MspA1I CmG'CkG Cuts at: 1606 2342 2682 2844 4126 4545 5222 6370 6615 Size: 736 340 162 1282 419 677 1148 245 Cuts at: 6615 1606 Size: 2120 Fragments arranged by size: 2120 1282 1148 736 677 419 340 245 162 NarI GG'CG_CC Cuts at: 4093 4093 Size: 7129 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6577 NdeI CA'TA_TG Cuts at: 387 387 Size: 7129 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7129 NotI GC'GGCC_GC Cuts at: 4576 4576 Size: 7129 NspI r_CATG'y Cuts at: 1132 1229 3368 3796 4487 6032 1132 Size: 97 2139 428 691 1545 2229 Fragments arranged by size: 2229 2139 1545 691 428 97 PasI CC'CwG_GG Cuts at: 1348 3934 1348 Size: 2586 4543 PciI A'CATG_T Cuts at: 3792 6028 3792 Size: 2236 4893 PflMI CCAn_nnn'nTGG Cuts at: 1108 2212 3334 1108 Size: 1104 1122 4903 Fragments arranged by size: 4903 1122 1104 PfoI T'CCnGG_A Cuts at: 2406 2466 3941 2406 Size: 60 1475 5594 Fragments arranged by size: 5594 1475 60 PmeI GTTT'AAAC Cuts at: 4433 4433 Size: 7129 PmlI CAC'GTG Cuts at: 2998 2998 Size: 7129 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5198 5230 6711 6743 5198 Size: 32 1481 32 5584 Fragments arranged by size: 5584 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3939 3939 Size: 7129 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7129 PsiI TTA'TAA Cuts at: 4699 4699 Size: 7129 PspOMI G'GGCC_C Cuts at: 4137 4189 4137 Size: 52 7077 PstI C_TGCA'G Cuts at: 830 4350 4481 830 Size: 3520 131 3478 Fragments arranged by size: 3520 3478 131 PvuI CG_AT'CG Cuts at: 2001 2241 2001 Size: 240 6889 PvuII CAG'CTG Cuts at: 2342 2844 2342 Size: 502 6627 SalI G'TCGA_C Cuts at: 1936 4471 1936 Size: 2535 4594 SbfI CC_TGCA'GG Cuts at: 4481 4481 Size: 7129 ScaI AGT'ACT Cuts at: 2623 5377 7089 2623 Size: 2754 1712 2663 Fragments arranged by size: 2754 2663 1712 SfcI C'TryA_G Cuts at: 826 1045 3916 4346 4477 5612 6293 6484 826 Size: 219 2871 430 131 1135 681 191 1471 Fragments arranged by size: 2871 1471 1135 681 430 219 191 131 SfoI GGC'GCC Cuts at: 4094 4094 Size: 7129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7129 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7129 SmaI CCC'GGG Cuts at: 1872 4424 4456 1872 Size: 2552 32 4545 Fragments arranged by size: 4545 2552 32 SmlI C'TyrA_G Cuts at: 820 1017 1943 3116 5233 6134 6396 820 Size: 197 926 1173 2117 901 262 1553 Fragments arranged by size: 2117 1553 1173 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4920 493 Size: 4427 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7129 SphI G_CATG'C Cuts at: 1132 3368 4487 1132 Size: 2236 1119 3774 Fragments arranged by size: 3774 2236 1119 SspI AAT'ATT Cuts at: 5 52 2698 5053 5 Size: 47 2646 2355 2081 Fragments arranged by size: 2646 2355 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7129 StyI C'CwwG_G Cuts at: 513 1065 2640 2955 3633 3713 4568 513 Size: 552 1575 315 678 80 855 3074 Fragments arranged by size: 3074 1575 855 678 552 315 80 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2143 2674 3322 3857 4211 5156 5173 Size: 1255 563 531 648 535 354 945 17 Cuts at: 5173 5326 5511 5956 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 945 648 563 535 531 445 354 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2621 2871 5375 Size: 274 80 33 51 1443 644 250 2504 Cuts at: 5375 7087 96 Size: 1712 138 Fragments arranged by size: 2504 1712 1443 644 274 250 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2293 2325 3791 3823 4397 4429 7089 7121 2293 Size: 32 1466 32 574 32 2660 32 2301 Fragments arranged by size: 2660 2301 1466 574 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7129 XbaI T'CTAG_A Cuts at: 4465 4465 Size: 7129 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2188 2277 3002 2188 Size: 89 725 6315 Fragments arranged by size: 6315 725 89 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7129 XmaI C'CCGG_G Cuts at: 1870 4422 4454 1870 Size: 2552 32 4545 Fragments arranged by size: 4545 2552 32 XmnI GAAnn'nnTTC Cuts at: 2252 5258 2252 Size: 3006 4123 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4937 276 Size: 53 83 186 936 3403 2468 Fragments arranged by size: 3403 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BsiWI EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI