(Circular) (Six-base) MAPSORT of: pFN21AA1472.seq Check: 2586 from: 1 to: 6694 pFN21AA1472 6694 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4504 278 Size: 53 83 186 936 2968 2468 Fragments arranged by size: 2968 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2498 4037 1937 Size: 561 1539 4594 Fragments arranged by size: 4594 1539 561 Acc65I G'GTAC_C Cuts at: 4015 4015 Size: 6694 AclI AA'CG_TT Cuts at: 1563 4821 5194 1563 Size: 3258 373 3063 Fragments arranged by size: 3258 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2879 3107 4757 6141 6409 1632 Size: 1247 228 1650 1384 268 1917 Fragments arranged by size: 1917 1650 1384 1247 268 228 AflII C'TTAA_G Cuts at: 820 1017 2909 820 Size: 197 1892 4605 Fragments arranged by size: 4605 1892 197 AflIII A'CryG_T Cuts at: 1203 2754 5593 6570 1203 Size: 1551 2839 977 1327 Fragments arranged by size: 2839 1551 1327 977 AgeI A'CCGG_T Cuts at: 5525 5525 Size: 6694 AhdI GACnn_n'nnGTC Cuts at: 2617 5423 2617 Size: 2806 3888 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6694 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6694 AlwNI CAG_nnn'CTG Cuts at: 2138 2226 2291 3362 3939 4437 6009 2138 Size: 88 65 1071 577 498 1572 2823 Fragments arranged by size: 2823 1572 1071 577 498 88 65 ApaI G_GGCC'C Cuts at: 2013 2013 Size: 6694 ApaLI G'TGCA_C Cuts at: 4751 5907 4751 Size: 1156 5538 ApoI r'AATT_y Cuts at: 1493 2076 4003 4229 4381 6490 1493 Size: 583 1927 226 152 2109 1697 Fragments arranged by size: 2109 1927 1697 583 226 152 AseI AT'TA_AT Cuts at: 160 1032 5248 160 Size: 872 4216 1606 Fragments arranged by size: 4216 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6694 AvaI C'yCGr_G Cuts at: 1870 1943 2455 3550 4019 1870 Size: 73 512 1095 469 4545 Fragments arranged by size: 4545 1095 512 469 73 BamHI G'GATC_C Cuts at: 3904 4024 4391 3904 Size: 120 367 6207 Fragments arranged by size: 6207 367 120 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3481 4015 5470 618 Size: 325 217 627 1694 534 1455 1842 Fragments arranged by size: 1842 1694 1455 627 534 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2648 3510 4013 1391 Size: 622 635 862 503 4072 Fragments arranged by size: 4072 862 635 622 503 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2282 2511 928 Size: 971 383 229 5111 Fragments arranged by size: 5111 971 383 229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4885 4919 1903 Size: 34 2948 34 3678 Fragments arranged by size: 3678 2948 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4586 5796 4586 Size: 1210 5484 BclI T'GATC_A Cuts at: 1548 1548 Size: 6694 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2307 2978 5305 Size: 107 122 71 631 605 635 671 2327 Cuts at: 5305 136 Size: 1525 Fragments arranged by size: 2327 1525 671 635 631 605 122 107 71 BglII A'GATC_T Cuts at: 6689 6689 Size: 6694 BlpI GC'TnA_GC Cuts at: 2197 2642 4111 2197 Size: 445 1469 4780 Fragments arranged by size: 4780 1469 445 Bme1580I G_kGCm'C Cuts at: 1790 2013 3486 4755 5911 1790 Size: 223 1473 1269 1156 2573 Fragments arranged by size: 2573 1473 1269 1156 223 BmgBI CAC'GTC Cuts at: 1318 3478 1318 Size: 2160 4534 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2658 2968 4425 Size: 416 53 83 399 24 1241 310 1457 Cuts at: 4425 5383 442 Size: 958 1753 Fragments arranged by size: 1753 1457 1241 958 416 399 310 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6694 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3628 3628 Size: 6694 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3131 3392 3428 3518 3832 5354 1239 Size: 1892 261 36 90 314 1522 2579 Fragments arranged by size: 2579 1892 1522 314 261 90 36 Bpu10I CC'TnA_GC Cuts at: 3932 3932 Size: 6694 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4819 5684 5982 4819 Size: 865 298 5531 Fragments arranged by size: 5531 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3671 5357 882 Size: 617 2172 1686 2219 Fragments arranged by size: 2219 2172 1686 617 BsaAI yAC'GTr Cuts at: 493 1204 4485 493 Size: 711 3281 2702 Fragments arranged by size: 3281 2702 711 BsaBI GATnn'nnATC Cuts at: 6688 6688 Size: 6694 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3560 3795 4501 4883 Size: 53 83 186 936 2027 235 706 382 Cuts at: 4883 5518 275 Size: 635 1451 Fragments arranged by size: 2027 1451 936 706 635 382 235 186 83 53 BsaWI w'CCGG_w Cuts at: 5127 5525 5799 5946 6357 5127 Size: 398 274 147 411 5464 Fragments arranged by size: 5464 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2687 2717 2897 2927 1178 Size: 30 165 30 1284 30 180 30 4945 Fragments arranged by size: 4945 1284 180 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2443 2963 2966 3452 1186 Size: 1257 520 3 486 4428 Fragments arranged by size: 4428 1257 520 486 3 BseYI C'CCAG_C Cuts at: 2131 2180 2916 3493 5897 6393 2131 Size: 49 736 577 2404 496 2432 Fragments arranged by size: 2432 2404 736 577 496 49 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2063 2650 1163 Size: 573 327 587 5207 Fragments arranged by size: 5207 587 573 327 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4144 4905 5933 1540 Size: 275 186 2143 761 1028 2301 Fragments arranged by size: 2301 2143 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2037 3510 4755 4840 5911 2037 Size: 1473 1245 85 1071 2820 Fragments arranged by size: 2820 1473 1245 1071 85 BsmI GAATG_Cn' Cuts at: 2379 3066 3382 3878 3958 4205 4298 2379 Size: 687 316 496 80 247 93 4775 Fragments arranged by size: 4775 687 496 316 247 93 80 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2037 2648 3486 3510 4013 4755 Size: 399 223 24 611 838 24 503 742 Cuts at: 4755 4840 5911 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 838 742 611 503 399 223 85 24 24 BspEI T'CCGG_A Cuts at: 6357 6357 Size: 6694 BspHI T'CATG_A Cuts at: 2050 2733 3723 4583 6313 2050 Size: 683 990 860 1730 2431 Fragments arranged by size: 2431 1730 990 860 683 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3250 4049 844 Size: 2406 799 3489 Fragments arranged by size: 3489 2406 799 BsrBI CCG'CTC Cuts at: 1394 1823 2480 4581 1394 Size: 429 657 2101 3507 Fragments arranged by size: 3507 2101 657 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5189 5363 65 Size: 1183 3941 174 1396 Fragments arranged by size: 3941 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5338 5525 1700 Size: 110 3528 187 2869 Fragments arranged by size: 3528 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6694 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6694 BssSI C'ACGA_G Cuts at: 4754 5766 4754 Size: 1012 5682 BstAPI GCAn_nnn'nTGC Cuts at: 794 2041 794 Size: 1247 5447 BstBI TT'CG_AA Cuts at: 5498 5498 Size: 6694 BstEII G'GTnAC_C Cuts at: 1184 3365 1184 Size: 2181 4513 BstXI CCAn_nnnn'nTGG Cuts at: 3427 3427 Size: 6694 BstYI r'GATC_y Cuts at: 1972 3832 3904 4024 4391 4776 4793 6245 6331 Size: 1860 72 120 367 385 17 1452 86 Cuts at: 6331 6343 6689 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1860 1452 385 367 346 120 86 72 17 12 Bsu36I CC'TnA_GG Cuts at: 2484 2484 Size: 6694 BtgI C'CryG_G Cuts at: 513 1065 2004 2600 3672 3855 3864 513 Size: 552 939 596 1072 183 9 3343 Fragments arranged by size: 3343 1072 939 596 552 183 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5955 Fragments arranged by size: 5955 683 56 BtsI GCAGTG_nn' Cuts at: 2813 3451 4210 5004 5024 2813 Size: 638 759 794 20 4483 Fragments arranged by size: 4483 794 759 638 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6694 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6659 DraI TTT'AAA Cuts at: 3998 3998 Size: 6694 DraIII CAC_nnn'GTG Cuts at: 2041 2125 3146 2041 Size: 84 1021 5589 Fragments arranged by size: 5589 1021 84 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5701 809 Size: 507 4385 1802 Fragments arranged by size: 4385 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4141 5030 6659 Size: 54 1425 50 275 18 2311 889 1629 Cuts at: 6659 8 Size: 43 Fragments arranged by size: 2311 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4141 1537 Size: 275 2329 4090 Fragments arranged by size: 4090 2329 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 3194 3249 3750 4624 1353 Size: 801 1040 55 501 874 3423 Fragments arranged by size: 3423 1040 874 801 501 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2566 3956 5281 5655 5801 387 Size: 1064 138 977 1390 1325 374 146 1280 Fragments arranged by size: 1390 1325 1280 1064 977 374 146 138 EcoICRI GAG'CTC Cuts at: 3508 3508 Size: 6694 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2879 3107 3131 3392 3428 3518 3832 Size: 393 1247 228 24 261 36 90 314 Cuts at: 3832 4757 5354 6141 6409 1239 Size: 925 597 787 268 1524 Fragments arranged by size: 1524 1247 925 787 597 393 314 268 261 228 90 36 24 EcoNI CCTnn'n_nnAGG Cuts at: 3931 3931 Size: 6694 EcoO109I rG'GnC_Cy Cuts at: 2009 2010 2540 2611 3538 3924 2009 Size: 1 530 71 927 386 4779 Fragments arranged by size: 4779 927 530 386 71 1 EcoRI G'AATT_C Cuts at: 2076 4003 4381 2076 Size: 1927 378 4389 Fragments arranged by size: 4389 1927 378 FspI TGC'GCA Cuts at: 5200 5200 Size: 6694 HaeII r_GCGC'y Cuts at: 1678 2197 2875 5841 1678 Size: 519 678 2966 2531 Fragments arranged by size: 2966 2531 678 519 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2435 2467 2603 2635 2897 Size: 32 135 32 863 32 136 32 262 Cuts at: 2897 2929 3230 3262 5335 5367 5409 5441 1373 Size: 32 301 32 2073 32 42 32 2626 Fragments arranged by size: 2626 2073 863 301 262 136 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2499 4038 4284 669 Size: 988 281 561 1539 246 3079 Fragments arranged by size: 3079 1539 988 561 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6694 HpaI GTT'AAC Cuts at: 4284 4284 Size: 6694 KpnI G_GTAC'C Cuts at: 4019 4019 Size: 6694 MfeI C'AATT_G Cuts at: 4293 4293 Size: 6694 MluI A'CGCG_T Cuts at: 6570 6570 Size: 6694 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3744 5808 5992 1305 Size: 2439 2064 184 2007 Fragments arranged by size: 2439 2064 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4872 Fragments arranged by size: 4872 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3134 3425 4652 5011 5170 518 Size: 648 1968 291 1227 359 159 2042 Fragments arranged by size: 2042 1968 1227 648 359 291 159 MspA1I CmG'CkG Cuts at: 1606 2602 2641 2920 4110 4787 5935 6180 1606 Size: 996 39 279 1190 677 1148 245 2120 Fragments arranged by size: 2120 1190 1148 996 677 279 245 39 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5203 Fragments arranged by size: 5203 939 552 NdeI CA'TA_TG Cuts at: 387 2103 2493 387 Size: 1716 390 4588 Fragments arranged by size: 4588 1716 390 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6694 NotI GC'GGCC_GC Cuts at: 4141 4141 Size: 6694 NspI r_CATG'y Cuts at: 1132 1229 2758 2932 4052 5597 1132 Size: 97 1529 174 1120 1545 2229 Fragments arranged by size: 2229 1545 1529 1120 174 97 PasI CC'CwG_GG Cuts at: 1348 3392 1348 Size: 2044 4650 PciI A'CATG_T Cuts at: 2754 5593 2754 Size: 2839 3855 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6694 PfoI T'CCnGG_A Cuts at: 3405 3534 3405 Size: 129 6565 PmeI GTTT'AAAC Cuts at: 3998 3998 Size: 6694 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4763 4795 6276 6308 4763 Size: 32 1481 32 5149 Fragments arranged by size: 5149 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2540 2611 3538 2540 Size: 71 927 5696 Fragments arranged by size: 5696 927 71 PshAI GACnn'nnGTC Cuts at: 1613 3543 1613 Size: 1930 4764 PsiI TTA'TAA Cuts at: 4264 4264 Size: 6694 PspOMI G'GGCC_C Cuts at: 2009 2009 Size: 6694 PspXI vC'TCGA_Gb Cuts at: 2455 3550 2455 Size: 1095 5599 PstI C_TGCA'G Cuts at: 830 2820 3528 4046 830 Size: 1990 708 518 3478 Fragments arranged by size: 3478 1990 708 518 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6694 PvuII CAG'CTG Cuts at: 2641 2920 2641 Size: 279 6415 SacI G_AGCT'C Cuts at: 3510 3510 Size: 6694 SacII CC_GC'GG Cuts at: 2603 2603 Size: 6694 SalI G'TCGA_C Cuts at: 1936 2497 4036 1936 Size: 561 1539 4594 Fragments arranged by size: 4594 1539 561 SapI GCTCTTCn'nnn_ Cuts at: 3194 3249 3194 Size: 55 6639 SbfI CC_TGCA'GG Cuts at: 4046 4046 Size: 6694 ScaI AGT'ACT Cuts at: 3809 3886 4942 6654 3809 Size: 77 1056 1712 3849 Fragments arranged by size: 3849 1712 1056 77 SexAI A'CCwGG_T Cuts at: 2428 2428 Size: 6694 SfcI C'TryA_G Cuts at: 826 1045 2722 2816 3487 3524 4042 5177 5858 Size: 219 1677 94 671 37 518 1135 681 Cuts at: 5858 6049 826 Size: 191 1471 Fragments arranged by size: 1677 1471 1135 681 671 518 219 191 94 37 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6694 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6694 SmaI CCC'GGG Cuts at: 1872 4021 1872 Size: 2149 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2455 2909 3550 4798 5699 5961 Size: 197 926 512 454 641 1248 901 262 Cuts at: 5961 820 Size: 1553 Fragments arranged by size: 1553 1248 926 901 641 512 454 262 197 SnaBI TAC'GTA Cuts at: 493 4485 493 Size: 3992 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6694 SphI G_CATG'C Cuts at: 1132 2932 4052 1132 Size: 1800 1120 3774 Fragments arranged by size: 3774 1800 1120 SspI AAT'ATT Cuts at: 5 52 3077 4618 5 Size: 47 3025 1541 2081 Fragments arranged by size: 3025 2081 1541 47 StuI AGG'CCT Cuts at: 1517 2418 1517 Size: 901 5793 StyI C'CwwG_G Cuts at: 513 1065 2004 4133 513 Size: 552 939 2129 3074 Fragments arranged by size: 3074 2129 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3488 4721 4738 4891 5076 5521 325 Size: 1255 1908 1233 17 153 185 445 1498 Fragments arranged by size: 1908 1498 1255 1233 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3807 3884 4940 Size: 274 80 33 51 1443 1830 77 1056 Cuts at: 4940 6652 96 Size: 1712 138 Fragments arranged by size: 1830 1712 1443 1056 274 138 80 77 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2111 2143 2230 2262 3152 3184 6654 6686 2111 Size: 32 87 32 890 32 3470 32 2119 Fragments arranged by size: 3470 2119 890 87 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6694 XbaI T'CTAG_A Cuts at: 4030 4030 Size: 6694 XhoI C'TCGA_G Cuts at: 1943 2455 3550 1943 Size: 512 1095 5087 Fragments arranged by size: 5087 1095 512 XmaI C'CCGG_G Cuts at: 1870 4019 1870 Size: 2149 4545 XmnI GAAnn'nnTTC Cuts at: 4823 4823 Size: 6694 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4502 276 Size: 53 83 186 936 2968 2468 Fragments arranged by size: 2968 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SanDI SfiI SfoI SrfI SwaI XcmI