Restriction map for
pFN21AA1469
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# NO CUT ENZYMES ( 129 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoO109I EcoRV FseI
FspI HaeII HgaI Hin1I HpaI
KasI KpnI KspI MluI MroI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SphI
Sse8387I SspI StuI SwaI Tth111I
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AlwNI 1727 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1012 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1141 TGGCCA TGG^CCA
BanI 783 GGTGCC G^G[CT][GA]CC
BanII 991 GGGCTC G[GA]GC[CT]^C
BcgI 813 GCAGCTCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1122 GTATCC GTATCC(6/5)
BclI 2 TGATCA T^GATCA
BcnI 1338 CCCGG CC^[GC]GG
BsaAI 947 TACGTG [CT]AC^GT[GA]
BsaBI 547 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 589 GGTCTC GGTCTC(1/5)
BsmI 445 GCATTC GAATGC(1/-1)
BstBI 1511 TTCGAA TT^CGAA
BstEII 1617 GGTTACC G^GT[ACGT]ACC
BstPI 1617 GGTTACC G^GT[ACGT]ACC
CfoI 8 GCGC GCG^C
DsaI 1442 CCATGG C^C[GA][CT]GG
EaeI 1141 TGGCCA [CT]^GGCC[GA]
EcoNI 1126 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1617 GGTTACC G^GT[ACGT]ACC
EcoRI 702 GAATTC G^AATTC
EcoT22I 1431 ATGCAT ATGCA^T
FbaI 2 TGATCA T^GATCA
HapII 1339 CCGG C^CGG
HhaI 8 GCGC GCG^C
HinP1I 8 GCGC G^CGC
HincII 979 GTCAAC GT[CT]^[GA]AC
HindII 979 GTCAAC GT[CT]^[GA]AC
HindIII 751 AAGCTT A^AGCTT
HpaII 1339 CCGG C^CGG
MamI 547 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1364 CAATTG C^AATTG
MluNI 1141 TGGCCA TGG^CCA
MscI 1141 TGGCCA TGG^CCA
MspA1I 1314 CAGCTG C[AC]G^C[GT]G
MspI 1339 CCGG C^CGG
MunI 1364 CAATTG C^AATTG
MwoI 1627 GCCCTTCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 1338 CCCGG CC^[GC]GG
NcoI 1442 CCATGG C^CATGG
NsiI 1431 ATGCAT ATGCA^T
NspV 1511 TTCGAA TT^CGAA
PstI 422 CTGCAG CTGCA^G
PvuII 1314 CAGCTG CAG^CTG
SapI 1671 GCTCTTC GCTCTTC(1/4)
ScaI 1388 AGTACT AGT^ACT
SfuI 1511 TTCGAA TT^CGAA
SpeI 595 ACTAGT A^CTAGT
XbaI 615 TCTAGA T^CTAGA
XcmI 1574 CCAATTTACCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1012 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AocI 744 CCTGAGG CC^T[ACGT]AGG
859 CCTCAGG
BmyI 784 GTGCCC G[AGT]GC[ACT]^C
991 GGGCTC
BseRI 1704 GAGGAG GAGGAG(10/8)
1819 GAGGAG
BsgI 716 CTGCAC GTGCAG(16/14)
1502 CTGCAC
Bsp1286I 784 GTGCCC G[AGT]GC[ACT]^C
991 GGGCTC
Bsp1407I 1517 TGTACA T^GTACA
1865 TGTACA
BsrDI 1623 CATTGC GCAATG(2/0)
1799 GCAATG
BsrGI 1517 TGTACA T^GTACA
1865 TGTACA
BstYI 1038 GGATCT [GA]^GATC[CT]
1348 GGATCT
Bsu36I 744 CCTGAGG CC^T[ACGT]AGG
859 CCTCAGG
Cfr13I 1329 GGGCC G^G[ACGT]CC
1611 GGCCC
DrdI 409 GACAACTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
681 GACAGAGCTGTC
Eco81I 744 CCTGAGG CC^T[ACGT]AGG
859 CCTCAGG
EcoT14I 1131 CCAAGG C^C[AT][AT]GG
1442 CCATGG
FauI 115 GCGGG CCCGC(4/6)
926 GCGGG
MaeII 948 ACGT A^CGT
983 ACGT
MflI 1038 GGATCT [GA]^GATC[CT]
1348 GGATCT
Sau96I 1329 GGGCC G^G[ACGT]CC
1611 GGCCC
SmlI 1218 CTCAAG C^T[CT][GA]AG
1677 CTCAAG
SspBI 1517 TGTACA T^GTACA
1865 TGTACA
StyI 1131 CCAAGG C^C[AT][AT]GG
1442 CCATGG
XhoII 1038 GGATCT [GA]^GATC[CT]
1348 GGATCT
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BpmI BsaJI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EarI EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV PleI RsaI Sau3AI ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I