(Circular) (Six-base) MAPSORT of: pFN21AA1469.seq Check: 4062 from: 1 to: 6652 pFN21AA1469 6652 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4462 278 Size: 53 83 186 936 2926 2468 Fragments arranged by size: 2926 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3995 1937 Size: 2058 4594 Acc65I G'GTAC_C Cuts at: 3973 3973 Size: 6652 AclI AA'CG_TT Cuts at: 1563 4779 5152 1563 Size: 3216 373 3063 Fragments arranged by size: 3216 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2183 2217 4715 6099 6367 1632 Size: 551 34 2498 1384 268 1917 Fragments arranged by size: 2498 1917 1384 551 268 34 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6455 AflIII A'CryG_T Cuts at: 1203 5551 6528 1203 Size: 4348 977 1327 Fragments arranged by size: 4348 1327 977 AgeI A'CCGG_T Cuts at: 5483 5483 Size: 6652 AhdI GACnn_n'nnGTC Cuts at: 5381 5381 Size: 6652 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6652 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6652 AlwNI CAG_nnn'CTG Cuts at: 3737 4395 5967 3737 Size: 658 1572 4422 Fragments arranged by size: 4422 1572 658 ApaLI G'TGCA_C Cuts at: 4709 5865 4709 Size: 1156 5496 ApoI r'AATT_y Cuts at: 1493 2294 2382 2707 3961 4187 4339 6448 1493 Size: 801 88 325 1254 226 152 2109 1697 Fragments arranged by size: 2109 1697 1254 801 325 226 152 88 AseI AT'TA_AT Cuts at: 160 1032 5206 160 Size: 872 4174 1606 Fragments arranged by size: 4174 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6652 AvaI C'yCGr_G Cuts at: 1870 1943 3977 1870 Size: 73 2034 4545 Fragments arranged by size: 4545 2034 73 BamHI G'GATC_C Cuts at: 3982 4349 3982 Size: 367 6285 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2788 3973 5428 618 Size: 325 217 627 1001 1185 1455 1842 Fragments arranged by size: 1842 1455 1185 1001 627 325 217 BanII G_rGCy'C Cuts at: 1391 3000 3971 1391 Size: 1609 971 4072 Fragments arranged by size: 4072 1609 971 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5681 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2807 2841 4843 4877 1903 Size: 34 870 34 2002 34 3678 Fragments arranged by size: 3678 2002 870 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3138 4544 5754 3138 Size: 1406 1210 4036 Fragments arranged by size: 4036 1406 1210 BclI T'GATC_A Cuts at: 1548 2007 1548 Size: 459 6193 BfrBI ATG'CAT Cuts at: 3438 3438 Size: 6652 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5263 136 Size: 107 122 71 631 605 3591 1525 Fragments arranged by size: 3591 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6647 6647 Size: 6652 BlpI GC'TnA_GC Cuts at: 4069 4069 Size: 6652 Bme1580I G_kGCm'C Cuts at: 1790 2793 4713 5869 1790 Size: 1003 1920 1156 2573 Fragments arranged by size: 2573 1920 1156 1003 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6652 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3341 4383 5341 Size: 416 53 83 399 24 1924 1042 958 Cuts at: 5341 442 Size: 1753 Fragments arranged by size: 1924 1753 1042 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6652 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2041 3138 3431 5312 1239 Size: 802 1097 293 1881 2579 Fragments arranged by size: 2579 1881 1097 802 293 Bpu10I CC'TnA_GC Cuts at: 2501 2501 Size: 6652 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3208 3667 4777 5642 5940 3208 Size: 459 1110 865 298 3920 Fragments arranged by size: 3920 1110 865 459 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2600 5315 882 Size: 617 1101 2715 2219 Fragments arranged by size: 2715 2219 1101 617 BsaAI yAC'GTr Cuts at: 493 1204 2954 4443 493 Size: 711 1750 1489 2702 Fragments arranged by size: 2702 1750 1489 711 BsaBI GATnn'nnATC Cuts at: 2556 6646 2556 Size: 4090 2562 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4459 4841 5476 275 Size: 53 83 186 936 2926 382 635 1451 Fragments arranged by size: 2926 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5085 5483 5757 5904 6315 5085 Size: 398 274 147 411 5422 Fragments arranged by size: 5422 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6427 Fragments arranged by size: 6427 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3724 3839 1186 Size: 2538 115 3999 Fragments arranged by size: 3999 2538 115 BseYI C'CCAG_C Cuts at: 2222 5855 6351 2222 Size: 3633 496 2523 Fragments arranged by size: 3633 2523 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2706 3492 1163 Size: 573 970 786 4323 Fragments arranged by size: 4323 970 786 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4102 4863 5891 1540 Size: 275 186 2101 761 1028 2301 Fragments arranged by size: 2301 2101 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4713 4798 5869 4713 Size: 85 1071 5496 Fragments arranged by size: 5496 1071 85 BsmI GAATG_Cn' Cuts at: 2450 4163 4256 2450 Size: 1713 93 4846 Fragments arranged by size: 4846 1713 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2793 3000 3971 4713 4798 5869 1391 Size: 399 1003 207 971 742 85 1071 2174 Fragments arranged by size: 2174 1071 1003 971 742 399 207 85 BspEI T'CCGG_A Cuts at: 6315 6315 Size: 6652 BspHI T'CATG_A Cuts at: 4541 6271 4541 Size: 1730 4922 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4007 844 Size: 3163 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4539 1394 Size: 429 2716 3507 Fragments arranged by size: 3507 2716 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3627 3811 5147 5321 65 Size: 1183 2379 184 1336 174 1396 Fragments arranged by size: 2379 1396 1336 1183 184 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5296 5483 1700 Size: 110 3486 187 2869 Fragments arranged by size: 3486 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3522 3870 96 Size: 3426 348 2878 Fragments arranged by size: 3426 2878 348 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6652 BssSI C'ACGA_G Cuts at: 4712 5724 4712 Size: 1012 5640 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6652 BstBI TT'CG_AA Cuts at: 3517 5456 3517 Size: 1939 4713 BstEII G'GTnAC_C Cuts at: 1184 3622 1184 Size: 2438 4214 BstYI r'GATC_y Cuts at: 1972 3043 3353 3982 4349 4734 4751 6203 6289 Size: 1071 310 629 367 385 17 1452 86 Cuts at: 6289 6301 6647 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1071 629 385 367 346 310 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2750 2865 2750 Size: 115 6537 BtgI C'CryG_G Cuts at: 513 1065 3447 513 Size: 552 2382 3718 Fragments arranged by size: 3718 2382 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2130 487 Size: 683 56 904 5009 Fragments arranged by size: 5009 904 683 56 BtsI GCAGTG_nn' Cuts at: 3105 3394 4168 4962 4982 3105 Size: 289 774 794 20 4775 Fragments arranged by size: 4775 794 774 289 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6652 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2974 3009 3125 3160 529 Size: 35 2410 35 116 35 4021 Fragments arranged by size: 4021 2410 116 35 35 35 DraI TTT'AAA Cuts at: 3956 3956 Size: 6652 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2420 2692 5659 809 Size: 507 1104 272 2967 1802 Fragments arranged by size: 2967 1802 1104 507 272 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3146 4099 4988 Size: 54 1425 50 275 18 1316 953 889 Cuts at: 4988 6617 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1316 953 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4099 1537 Size: 275 2287 4090 Fragments arranged by size: 4090 2287 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2436 2592 3683 4582 1353 Size: 1083 156 1091 899 3423 Fragments arranged by size: 3423 1091 1083 899 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5239 5613 5759 387 Size: 1064 138 3650 374 146 1280 Fragments arranged by size: 3650 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 2183 2217 3138 3431 4715 5312 Size: 393 409 142 34 921 293 1284 597 Cuts at: 5312 6099 6367 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1284 921 787 597 409 393 293 268 142 34 EcoNI CCTnn'n_nnAGG Cuts at: 3135 3135 Size: 6652 EcoRI G'AATT_C Cuts at: 2707 3961 4339 2707 Size: 1254 378 5020 Fragments arranged by size: 5020 1254 378 FspI TGC'GCA Cuts at: 5158 5158 Size: 6652 HaeII r_GCGC'y Cuts at: 1678 5799 1678 Size: 4121 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2402 2434 5293 5325 5367 Size: 32 135 32 830 32 2859 32 42 Cuts at: 5367 5399 1373 Size: 32 2626 Fragments arranged by size: 2859 2626 830 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2986 3996 4242 669 Size: 988 281 1048 1010 246 3079 Fragments arranged by size: 3079 1048 1010 988 281 246 HindIII A'AGCT_T Cuts at: 748 2756 748 Size: 2008 4644 HpaI GTT'AAC Cuts at: 4242 4242 Size: 6652 KpnI G_GTAC'C Cuts at: 3977 3977 Size: 6652 MfeI C'AATT_G Cuts at: 3369 4251 3369 Size: 882 5770 MluI A'CGCG_T Cuts at: 6528 6528 Size: 6652 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2186 2497 5766 5950 1305 Size: 881 311 3269 184 2007 Fragments arranged by size: 3269 2007 881 311 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3148 10 Size: 54 1425 343 1316 3514 Fragments arranged by size: 3514 1425 1316 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2104 2409 3666 4610 4969 5128 518 Size: 648 938 305 1257 944 359 159 2042 Fragments arranged by size: 2042 1257 944 938 648 359 305 159 MspA1I CmG'CkG Cuts at: 1606 3321 4068 4745 5893 6138 1606 Size: 1715 747 677 1148 245 2120 Fragments arranged by size: 2120 1715 1148 747 677 245 NcoI C'CATG_G Cuts at: 513 1065 3447 513 Size: 552 2382 3718 Fragments arranged by size: 3718 2382 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6652 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6652 NotI GC'GGCC_GC Cuts at: 4099 4099 Size: 6652 NsiI A_TGCA'T Cuts at: 3440 3440 Size: 6652 NspI r_CATG'y Cuts at: 1132 1229 4010 5555 1132 Size: 97 2781 1545 2229 Fragments arranged by size: 2781 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2514 1348 Size: 1166 5486 PciI A'CATG_T Cuts at: 5551 5551 Size: 6652 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6652 PfoI T'CCnGG_A Cuts at: 3762 3762 Size: 6652 PmeI GTTT'AAAC Cuts at: 3956 3956 Size: 6652 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4721 4753 6234 6266 4721 Size: 32 1481 32 5107 Fragments arranged by size: 5107 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6652 PsiI TTA'TAA Cuts at: 4222 4222 Size: 6652 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3426 3458 3426 Size: 32 6620 PstI C_TGCA'G Cuts at: 830 2431 4004 830 Size: 1601 1573 3478 Fragments arranged by size: 3478 1601 1573 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6652 PvuII CAG'CTG Cuts at: 3321 3321 Size: 6652 SalI G'TCGA_C Cuts at: 1936 3994 1936 Size: 2058 4594 SapI GCTCTTCn'nnn_ Cuts at: 3683 3683 Size: 6652 SbfI CC_TGCA'GG Cuts at: 4004 4004 Size: 6652 ScaI AGT'ACT Cuts at: 3395 4900 6612 3395 Size: 1505 1712 3435 Fragments arranged by size: 3435 1712 1505 SfcI C'TryA_G Cuts at: 826 1045 2427 2532 3910 4000 5135 5816 6007 Size: 219 1382 105 1378 90 1135 681 191 Cuts at: 6007 826 Size: 1471 Fragments arranged by size: 1471 1382 1378 1135 681 219 191 105 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6652 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6652 SmaI CCC'GGG Cuts at: 1872 3979 1872 Size: 2107 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3223 3682 4756 5657 5919 820 Size: 197 926 1280 459 1074 901 262 1553 Fragments arranged by size: 1553 1280 1074 926 901 459 262 197 SnaBI TAC'GTA Cuts at: 493 4443 493 Size: 3950 2702 SpeI A'CTAG_T Cuts at: 152 2600 152 Size: 2448 4204 SphI G_CATG'C Cuts at: 1132 4010 1132 Size: 2878 3774 SspI AAT'ATT Cuts at: 5 52 4576 5 Size: 47 4524 2081 Fragments arranged by size: 4524 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6652 StyI C'CwwG_G Cuts at: 513 1065 3136 3447 4091 513 Size: 552 2071 311 644 3074 Fragments arranged by size: 3074 2071 644 552 311 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4679 4696 4849 5034 5479 325 Size: 1255 3099 17 153 185 445 1498 Fragments arranged by size: 3099 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3393 3522 3870 Size: 274 80 33 51 1443 1416 129 348 Cuts at: 3870 4898 6610 96 Size: 1028 1712 138 Fragments arranged by size: 1712 1443 1416 1028 348 274 138 129 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3112 3144 3202 3234 3832 3864 6612 6644 3112 Size: 32 58 32 598 32 2748 32 3120 Fragments arranged by size: 3120 2748 598 58 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6652 XbaI T'CTAG_A Cuts at: 2620 3988 2620 Size: 1368 5284 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3586 3586 Size: 6652 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6652 XmaI C'CCGG_G Cuts at: 1870 3977 1870 Size: 2107 4545 XmnI GAAnn'nnTTC Cuts at: 3021 4781 3021 Size: 1760 4892 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4460 276 Size: 53 83 186 936 2926 2468 Fragments arranged by size: 2926 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI