Restriction map for
pFN21AA1469
(Linear) (Six-base) MAPSORT of: KIAA1469.seq Check: 4998 from: 1 to: 1947
KIAA1469.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 212 1947
Size: 178 34 1735
Fragments arranged by size:
1735 178 34
AlwNI CAG_nnn'CTG
Cuts at: 0 1732 1947
Size: 1732 215
ApoI r'AATT_y
Cuts at: 0 289 377 702 1947
Size: 289 88 325 1245
Fragments arranged by size:
1245 325 289 88
BanI G'GyrC_C
Cuts at: 0 783 1947
Size: 783 1164
BanII G_rGCy'C
Cuts at: 0 995 1947
Size: 995 952
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 802 836 1947
Size: 802 34 1111
Fragments arranged by size:
1111 802 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1133 1947
Size: 1133 814
BclI T'GATC_A
Cuts at: 0 2 1947
Size: 2 1945
BfrBI ATG'CAT
Cuts at: 0 1433 1947
Size: 1433 514
Bme1580I G_kGCm'C
Cuts at: 0 788 1947
Size: 788 1159
BmrI ACTGGGnnnn_n'
Cuts at: 0 1336 1947
Size: 1336 611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 1133 1426 1947
Size: 36 1097 293 521
Fragments arranged by size:
1097 521 293 36
Bpu10I CC'TnA_GC
Cuts at: 0 496 1947
Size: 496 1451
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1203 1662 1947
Size: 1203 459 285
Fragments arranged by size:
1203 459 285
BsaI GGTCTCn'nnnn_
Cuts at: 0 595 1947
Size: 595 1352
BsaAI yAC'GTr
Cuts at: 0 949 1947
Size: 949 998
BsaBI GATnn'nnATC
Cuts at: 0 551 1947
Size: 551 1396
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1719 1834 1947
Size: 1719 115 113
Fragments arranged by size:
1719 115 113
BseYI C'CCAG_C
Cuts at: 0 217 1947
Size: 217 1730
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1487 1947
Size: 701 786 460
Fragments arranged by size:
786 701 460
BsmI GAATG_Cn'
Cuts at: 0 445 1947
Size: 445 1502
Bsp1286I G_dGCh'C
Cuts at: 0 788 995 1947
Size: 788 207 952
Fragments arranged by size:
952 788 207
BsrDI GCAATG_nn'
Cuts at: 0 1622 1806 1947
Size: 1622 184 141
Fragments arranged by size:
1622 184 141
BsrGI T'GTAC_A
Cuts at: 0 1517 1865 1947
Size: 1517 348 82
Fragments arranged by size:
1517 348 82
BstBI TT'CG_AA
Cuts at: 0 1512 1947
Size: 1512 435
BstEII G'GTnAC_C
Cuts at: 0 1617 1947
Size: 1617 330
BstYI r'GATC_y
Cuts at: 0 1038 1348 1947
Size: 1038 310 599
Fragments arranged by size:
1038 599 310
Bsu36I CC'TnA_GG
Cuts at: 0 745 860 1947
Size: 745 115 1087
Fragments arranged by size:
1087 745 115
BtgI C'CryG_G
Cuts at: 0 1442 1947
Size: 1442 505
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 125 1947
Size: 125 1822
BtsI GCAGTG_nn'
Cuts at: 0 1100 1389 1947
Size: 1100 289 558
Fragments arranged by size:
1100 558 289
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 969 1004 1120 1155 1947
Size: 969 35 116 35 792
Fragments arranged by size:
969 792 116 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 415 687 1947
Size: 415 272 1260
Fragments arranged by size:
1260 415 272
EaeI y'GGCC_r
Cuts at: 0 1141 1947
Size: 1141 806
EarI CTCTTCn'nnn_
Cuts at: 0 431 587 1678 1947
Size: 431 156 1091 269
Fragments arranged by size:
1091 431 269 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 178 212 1133 1426 1947
Size: 36 142 34 921 293 521
Fragments arranged by size:
921 521 293 142 36 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1130 1947
Size: 1130 817
EcoRI G'AATT_C
Cuts at: 0 702 1947
Size: 702 1245
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 1947
Size: 397 32 1518
Fragments arranged by size:
1518 397 32
HincII GTy'rAC
Cuts at: 0 981 1947
Size: 981 966
HindIII A'AGCT_T
Cuts at: 0 751 1947
Size: 751 1196
MfeI C'AATT_G
Cuts at: 0 1364 1947
Size: 1364 583
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 492 1947
Size: 181 311 1455
Fragments arranged by size:
1455 311 181
MscI TGG'CCA
Cuts at: 0 1143 1947
Size: 1143 804
MslI CAynn'nnrTG
Cuts at: 0 99 404 1661 1947
Size: 99 305 1257 286
Fragments arranged by size:
1257 305 286 99
MspA1I CmG'CkG
Cuts at: 0 1316 1947
Size: 1316 631
NcoI C'CATG_G
Cuts at: 0 1442 1947
Size: 1442 505
NsiI A_TGCA'T
Cuts at: 0 1435 1947
Size: 1435 512
PasI CC'CwG_GG
Cuts at: 0 509 1947
Size: 509 1438
PfoI T'CCnGG_A
Cuts at: 0 1757 1947
Size: 1757 190
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1421 1453 1947
Size: 1421 32 494
Fragments arranged by size:
1421 494 32
PstI C_TGCA'G
Cuts at: 0 426 1947
Size: 426 1521
PvuII CAG'CTG
Cuts at: 0 1316 1947
Size: 1316 631
SapI GCTCTTCn'nnn_
Cuts at: 0 1678 1947
Size: 1678 269
ScaI AGT'ACT
Cuts at: 0 1390 1947
Size: 1390 557
SfcI C'TryA_G
Cuts at: 0 422 527 1905 1947
Size: 422 105 1378 42
Fragments arranged by size:
1378 422 105 42
SmlI C'TyrA_G
Cuts at: 0 1218 1677 1947
Size: 1218 459 270
Fragments arranged by size:
1218 459 270
SpeI A'CTAG_T
Cuts at: 0 595 1947
Size: 595 1352
StyI C'CwwG_G
Cuts at: 0 1131 1442 1947
Size: 1131 311 505
Fragments arranged by size:
1131 505 311
TatI w'GTAC_w
Cuts at: 0 1388 1517 1865 1947
Size: 1388 129 348 82
Fragments arranged by size:
1388 348 129 82
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1107 1139 1197 1229 1827 1859 1947
Size: 1107 32 58 32 598 32 88
Fragments arranged by size:
1107 598 88 58 32 32 32
XbaI T'CTAG_A
Cuts at: 0 615 1947
Size: 615 1332
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1581 1947
Size: 1581 366
XmnI GAAnn'nnTTC
Cuts at: 0 1016 1947
Size: 1016 931
Enzymes that do cut:
AcuI AlwNI ApoI BanI BanII BcgI BciVI BclI
BfrBI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BseRI BseYI BsgI BsmI Bsp1286I BsrDI BsrGI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI CspCI
DrdI EaeI EarI Eco57MI EcoNI EcoRI Hin4I HincII
HindIII MfeI MmeI MscI MslI MspA1I NcoI NsiI
PasI PfoI PsrI PstI PvuII SapI ScaI SfcI
SmlI SpeI StyI TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BbvCI BglI BglII BlpI BmgBI BmtI BplI BsaHI
BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstZ17I
ClaI DraI DraIII EagI EciI EcoICRI EcoO109I EcoRV
FalI FseI FspI FspAI HaeII HpaI KasI KpnI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII
SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SspI StuI SwaI TaqII
Tth111I XhoI XmaI ZraI