(Circular) (Six-base) MAPSORT of: pFN21AA1451.seq Check: 7707 from: 1 to: 7372 pFN21AA1451 7372 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3164 3164 Size: 7372 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5182 278 Size: 53 83 186 936 3646 2468 Fragments arranged by size: 3646 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4715 1937 Size: 2778 4594 Acc65I G'GTAC_C Cuts at: 4693 4693 Size: 7372 AclI AA'CG_TT Cuts at: 1563 5499 5872 1563 Size: 3936 373 3063 Fragments arranged by size: 3936 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2733 3225 3464 4125 5435 6819 7087 1632 Size: 1101 492 239 661 1310 1384 268 1917 Fragments arranged by size: 1917 1384 1310 1101 661 492 268 239 AflII C'TTAA_G Cuts at: 820 1017 2366 820 Size: 197 1349 5826 Fragments arranged by size: 5826 1349 197 AflIII A'CryG_T Cuts at: 1203 2875 3785 6271 7248 1203 Size: 1672 910 2486 977 1327 Fragments arranged by size: 2486 1672 1327 977 910 AgeI A'CCGG_T Cuts at: 6203 6203 Size: 7372 AhdI GACnn_n'nnGTC Cuts at: 6101 6101 Size: 7372 AleI CACnn'nnGTG Cuts at: 1166 2876 2919 1166 Size: 1710 43 5619 Fragments arranged by size: 5619 1710 43 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7372 AlwNI CAG_nnn'CTG Cuts at: 2116 3197 4409 5115 6687 2116 Size: 1081 1212 706 1572 2801 Fragments arranged by size: 2801 1572 1212 1081 706 ApaLI G'TGCA_C Cuts at: 5429 6585 5429 Size: 1156 6216 ApoI r'AATT_y Cuts at: 1493 3735 3818 4025 4681 4907 5059 7168 1493 Size: 2242 83 207 656 226 152 2109 1697 Fragments arranged by size: 2242 2109 1697 656 226 207 152 83 AseI AT'TA_AT Cuts at: 160 1032 4007 5926 160 Size: 872 2975 1919 1606 Fragments arranged by size: 2975 1919 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7372 AvaI C'yCGr_G Cuts at: 1870 1943 4077 4697 1870 Size: 73 2134 620 4545 Fragments arranged by size: 4545 2134 620 73 AvrII C'CTAG_G Cuts at: 3838 3838 Size: 7372 BamHI G'GATC_C Cuts at: 2699 4702 5069 2699 Size: 2003 367 5002 Fragments arranged by size: 5002 2003 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4693 6148 618 Size: 325 217 627 2906 1455 1842 Fragments arranged by size: 2906 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4691 1391 Size: 3300 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2801 928 Size: 971 902 5499 Fragments arranged by size: 5499 971 902 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5563 5597 1903 Size: 34 3626 34 3678 Fragments arranged by size: 3678 3626 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3620 3794 5264 6474 3620 Size: 174 1470 1210 4518 Fragments arranged by size: 4518 1470 1210 174 BclI T'GATC_A Cuts at: 1548 2733 2826 3010 1548 Size: 1185 93 184 5910 Fragments arranged by size: 5910 1185 184 93 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4366 5983 136 Size: 107 122 71 631 605 2694 1617 1525 Fragments arranged by size: 2694 1617 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7367 7367 Size: 7372 BlpI GC'TnA_GC Cuts at: 4789 4789 Size: 7372 Bme1580I G_kGCm'C Cuts at: 1790 5433 6589 1790 Size: 3643 1156 2573 Fragments arranged by size: 3643 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7372 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3893 4493 5103 Size: 416 53 83 399 24 2476 600 610 Cuts at: 5103 6061 442 Size: 958 1753 Fragments arranged by size: 2476 1753 958 610 600 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7372 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3779 6032 1239 Size: 2540 2253 2579 Fragments arranged by size: 2579 2540 2253 Bpu10I CC'TnA_GC Cuts at: 2852 3108 3599 2852 Size: 256 491 6625 Fragments arranged by size: 6625 491 256 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5497 6362 6660 5497 Size: 865 298 6209 Fragments arranged by size: 6209 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6035 882 Size: 617 4536 2219 Fragments arranged by size: 4536 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2900 5163 493 Size: 711 1696 2263 2702 Fragments arranged by size: 2702 2263 1696 711 BsaBI GATnn'nnATC Cuts at: 2181 7366 2181 Size: 5185 2187 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5179 5561 6196 275 Size: 53 83 186 936 3646 382 635 1451 Fragments arranged by size: 3646 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5805 6203 6477 6624 7035 5805 Size: 398 274 147 411 6142 Fragments arranged by size: 6142 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7147 Fragments arranged by size: 7147 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4414 1186 Size: 3228 4144 BseYI C'CCAG_C Cuts at: 6575 7071 6575 Size: 496 6876 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6799 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4822 5583 6611 1540 Size: 275 186 2821 761 1028 2301 Fragments arranged by size: 2821 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5433 5518 6589 5433 Size: 85 1071 6216 Fragments arranged by size: 6216 1071 85 BsmI GAATG_Cn' Cuts at: 2590 4883 4976 2590 Size: 2293 93 4986 Fragments arranged by size: 4986 2293 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4691 5433 5518 6589 1391 Size: 399 2901 742 85 1071 2174 Fragments arranged by size: 2901 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7035 7035 Size: 7372 BspHI T'CATG_A Cuts at: 5261 6991 5261 Size: 1730 5642 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3164 4727 844 Size: 2320 1563 3489 Fragments arranged by size: 3489 2320 1563 BsrBI CCG'CTC Cuts at: 1394 1823 5259 1394 Size: 429 3436 3507 Fragments arranged by size: 3507 3436 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4004 5867 6041 65 Size: 1183 2756 1863 174 1396 Fragments arranged by size: 2756 1863 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4582 6016 6203 1700 Size: 110 2772 1434 187 2869 Fragments arranged by size: 2869 2772 1434 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7372 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7372 BssSI C'ACGA_G Cuts at: 5432 6444 5432 Size: 1012 6360 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7372 BstBI TT'CG_AA Cuts at: 4558 6176 4558 Size: 1618 5754 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7372 BstXI CCAn_nnnn'nTGG Cuts at: 2555 2555 Size: 7372 BstYI r'GATC_y Cuts at: 1972 2437 2699 4702 5069 5454 5471 6923 7009 Size: 465 262 2003 367 385 17 1452 86 Cuts at: 7009 7021 7367 1972 Size: 12 346 1977 Fragments arranged by size: 2003 1977 1452 465 385 367 346 262 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 4253 4587 513 Size: 552 939 2249 334 3298 Fragments arranged by size: 3298 2249 939 552 334 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6633 Fragments arranged by size: 6633 683 56 BtsI GCAGTG_nn' Cuts at: 2676 4888 5682 5702 2676 Size: 2212 794 20 4346 Fragments arranged by size: 4346 2212 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7372 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7337 DraI TTT'AAA Cuts at: 3126 3823 4676 3126 Size: 697 853 5822 Fragments arranged by size: 5822 853 697 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6379 809 Size: 507 5063 1802 Fragments arranged by size: 5063 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4584 4819 5708 Size: 54 1425 50 275 18 2754 235 889 Cuts at: 5708 7337 8 Size: 1629 43 Fragments arranged by size: 2754 1629 1425 889 275 235 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4819 1537 Size: 275 3007 4090 Fragments arranged by size: 4090 3007 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4173 4560 5302 1353 Size: 2820 387 742 3423 Fragments arranged by size: 3423 2820 742 387 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5959 6333 6479 387 Size: 1064 138 4370 374 146 1280 Fragments arranged by size: 4370 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2733 3225 3464 3779 4125 5435 6032 Size: 393 1101 492 239 315 346 1310 597 Cuts at: 6032 6819 7087 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1310 1101 787 597 492 393 346 315 268 239 EcoNI CCTnn'n_nnAGG Cuts at: 3266 3353 3266 Size: 87 7285 EcoO109I rG'GnC_Cy Cuts at: 4258 4258 Size: 7372 EcoRI G'AATT_C Cuts at: 3735 4681 5059 3735 Size: 946 378 6048 Fragments arranged by size: 6048 946 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2087 2381 2641 3755 2087 Size: 294 260 1114 5704 Fragments arranged by size: 5704 1114 294 260 FspI TGC'GCA Cuts at: 5878 5878 Size: 7372 HaeII r_GCGC'y Cuts at: 1678 2143 6519 1678 Size: 465 4376 2531 Fragments arranged by size: 4376 2531 465 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3335 3367 4034 4066 6013 Size: 32 135 32 1763 32 667 32 1947 Cuts at: 6013 6045 6087 6119 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1947 1763 667 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3675 4716 4962 669 Size: 988 281 1737 1041 246 3079 Fragments arranged by size: 3079 1737 1041 988 281 246 HindIII A'AGCT_T Cuts at: 748 2154 748 Size: 1406 5966 HpaI GTT'AAC Cuts at: 3675 4962 3675 Size: 1287 6085 KpnI G_GTAC'C Cuts at: 4697 4697 Size: 7372 MfeI C'AATT_G Cuts at: 4971 4971 Size: 7372 MluI A'CGCG_T Cuts at: 7248 7248 Size: 7372 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2186 2677 4004 6486 6670 1305 Size: 881 491 1327 2482 184 2007 Fragments arranged by size: 2482 2007 1327 881 491 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5550 Fragments arranged by size: 5550 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2876 2919 5330 5689 5848 518 Size: 648 1710 43 2411 359 159 2042 Fragments arranged by size: 2411 2042 1710 648 359 159 43 MspA1I CmG'CkG Cuts at: 1606 4788 5465 6613 6858 1606 Size: 3182 677 1148 245 2120 Fragments arranged by size: 3182 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 4253 513 Size: 552 939 2249 3632 Fragments arranged by size: 3632 2249 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7372 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7372 NotI GC'GGCC_GC Cuts at: 4819 4819 Size: 7372 NspI r_CATG'y Cuts at: 1132 1229 2879 3789 4730 6275 1132 Size: 97 1650 910 941 1545 2229 Fragments arranged by size: 2229 1650 1545 941 910 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7372 PciI A'CATG_T Cuts at: 2875 3785 6271 2875 Size: 910 2486 3976 Fragments arranged by size: 3976 2486 910 PflMI CCAn_nnn'nTGG Cuts at: 1108 2505 2650 1108 Size: 1397 145 5830 Fragments arranged by size: 5830 1397 145 PfoI T'CCnGG_A Cuts at: 3309 3309 Size: 7372 PmeI GTTT'AAAC Cuts at: 4676 4676 Size: 7372 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5441 5473 6954 6986 5441 Size: 32 1481 32 5827 Fragments arranged by size: 5827 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4258 4258 Size: 7372 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7372 PsiI TTA'TAA Cuts at: 2310 3456 4942 2310 Size: 1146 1486 4740 Fragments arranged by size: 4740 1486 1146 PspXI vC'TCGA_Gb Cuts at: 4077 4077 Size: 7372 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2559 2591 2559 Size: 32 7340 PstI C_TGCA'G Cuts at: 830 4724 830 Size: 3894 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7372 SalI G'TCGA_C Cuts at: 1936 4714 1936 Size: 2778 4594 SanDI GG'GwC_CC Cuts at: 4258 4258 Size: 7372 SapI GCTCTTCn'nnn_ Cuts at: 4560 4560 Size: 7372 SbfI CC_TGCA'GG Cuts at: 4724 4724 Size: 7372 ScaI AGT'ACT Cuts at: 5620 7332 5620 Size: 1712 5660 SfcI C'TryA_G Cuts at: 826 1045 4720 5855 6536 6727 826 Size: 219 3675 1135 681 191 1471 Fragments arranged by size: 3675 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7372 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7372 SmaI CCC'GGG Cuts at: 1872 4699 1872 Size: 2827 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2366 4077 5476 6377 6639 820 Size: 197 926 423 1711 1399 901 262 1553 Fragments arranged by size: 1711 1553 1399 926 901 423 262 197 SnaBI TAC'GTA Cuts at: 493 5163 493 Size: 4670 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7372 SphI G_CATG'C Cuts at: 1132 4730 1132 Size: 3598 3774 SspI AAT'ATT Cuts at: 5 52 3654 3781 4546 5296 5 Size: 47 3602 127 765 750 2081 Fragments arranged by size: 3602 2081 765 750 127 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7372 StyI C'CwwG_G Cuts at: 513 1065 2004 2071 2143 3267 3838 4253 4811 Size: 552 939 67 72 1124 571 415 558 Cuts at: 4811 513 Size: 3074 Fragments arranged by size: 3074 1124 939 571 558 552 415 72 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5399 5416 5569 5754 6199 325 Size: 1255 3819 17 153 185 445 1498 Fragments arranged by size: 3819 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2426 4457 5618 Size: 274 80 33 51 1443 449 2031 1161 Cuts at: 5618 7330 96 Size: 1712 138 Fragments arranged by size: 2031 1712 1443 1161 449 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7332 7364 7332 Size: 32 7340 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7372 XbaI T'CTAG_A Cuts at: 4708 4708 Size: 7372 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3161 3161 Size: 7372 XhoI C'TCGA_G Cuts at: 1943 4077 1943 Size: 2134 5238 XmaI C'CCGG_G Cuts at: 1870 4697 1870 Size: 2827 4545 XmnI GAAnn'nnTTC Cuts at: 5501 5501 Size: 7372 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5180 276 Size: 53 83 186 936 3646 2468 Fragments arranged by size: 3646 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PvuII RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI