Restriction map for
pFN21AA1451
(Linear) (Six-base) MAPSORT of: KIAA1451.seq Check: 9566 from: 1 to: 2667
KIAA1451.orf
With 182 enzymes: SgfI *
October 24, 2006 15:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1159 2667
Size: 1159 1508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 1220 1459 2120 2667
Size: 728 492 239 661 547
Fragments arranged by size:
728 661 547 492 239
AflII C'TTAA_G
Cuts at: 0 361 2667
Size: 361 2306
AflIII A'CryG_T
Cuts at: 0 870 1780 2667
Size: 870 910 887
Fragments arranged by size:
910 887 870
AleI CACnn'nnGTG
Cuts at: 0 871 914 2667
Size: 871 43 1753
Fragments arranged by size:
1753 871 43
AlwNI CAG_nnn'CTG
Cuts at: 0 111 1192 2404 2667
Size: 111 1081 1212 263
Fragments arranged by size:
1212 1081 263 111
ApoI r'AATT_y
Cuts at: 0 1730 1813 2020 2667
Size: 1730 83 207 647
Fragments arranged by size:
1730 647 207 83
AseI AT'TA_AT
Cuts at: 0 2002 2667
Size: 2002 665
AvaI C'yCGr_G
Cuts at: 0 2072 2667
Size: 2072 595
AvrII C'CTAG_G
Cuts at: 0 1833 2667
Size: 1833 834
BamHI G'GATC_C
Cuts at: 0 694 2667
Size: 694 1973
BbsI GAAGACnn'nnnn_
Cuts at: 0 796 2667
Size: 796 1871
BciVI GTATCCnnnnn_n'
Cuts at: 0 1615 1789 2667
Size: 1615 174 878
Fragments arranged by size:
1615 878 174
BclI T'GATC_A
Cuts at: 0 728 821 1005 2667
Size: 728 93 184 1662
Fragments arranged by size:
1662 728 184 93
BglI GCCn_nnn'nGGC
Cuts at: 0 2361 2667
Size: 2361 306
BmrI ACTGGGnnnn_n'
Cuts at: 0 1888 2488 2667
Size: 1888 600 179
Fragments arranged by size:
1888 600 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1774 2667
Size: 1774 893
Bpu10I CC'TnA_GC
Cuts at: 0 847 1103 1594 2667
Size: 847 256 491 1073
Fragments arranged by size:
1073 847 491 256
BsaAI yAC'GTr
Cuts at: 0 895 2667
Size: 895 1772
BsaBI GATnn'nnATC
Cuts at: 0 176 2667
Size: 176 2491
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2409 2667
Size: 2409 258
BsmI GAATG_Cn'
Cuts at: 0 585 2667
Size: 585 2082
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1159 2667
Size: 1159 1508
BsrDI GCAATG_nn'
Cuts at: 0 1999 2667
Size: 1999 668
BsrFI r'CCGG_y
Cuts at: 0 2577 2667
Size: 2577 90
BstBI TT'CG_AA
Cuts at: 0 2553 2667
Size: 2553 114
BstXI CCAn_nnnn'nTGG
Cuts at: 0 550 2667
Size: 550 2117
BstYI r'GATC_y
Cuts at: 0 432 694 2667
Size: 432 262 1973
Fragments arranged by size:
1973 432 262
BtgI C'CryG_G
Cuts at: 0 2248 2582 2667
Size: 2248 334 85
Fragments arranged by size:
2248 334 85
BtsI GCAGTG_nn'
Cuts at: 0 671 2667
Size: 671 1996
DraI TTT'AAA
Cuts at: 0 1121 1818 2667
Size: 1121 697 849
Fragments arranged by size:
1121 849 697
EaeI y'GGCC_r
Cuts at: 0 2579 2667
Size: 2579 88
EarI CTCTTCn'nnn_
Cuts at: 0 2168 2555 2667
Size: 2168 387 112
Fragments arranged by size:
2168 387 112
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 1220 1459 1774 2120 2667
Size: 728 492 239 315 346 547
Fragments arranged by size:
728 547 492 346 315 239
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1261 1348 2667
Size: 1261 87 1319
Fragments arranged by size:
1319 1261 87
EcoO109I rG'GnC_Cy
Cuts at: 0 2253 2667
Size: 2253 414
EcoRI G'AATT_C
Cuts at: 0 1730 2667
Size: 1730 937
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 82 376 636 1750 2667
Size: 82 294 260 1114 917
Fragments arranged by size:
1114 917 294 260 82
HaeII r_GCGC'y
Cuts at: 0 138 2667
Size: 138 2529
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1330 1362 2029 2061 2667
Size: 1330 32 667 32 606
Fragments arranged by size:
1330 667 606 32 32
HincII GTy'rAC
Cuts at: 0 1670 2667
Size: 1670 997
HindIII A'AGCT_T
Cuts at: 0 149 2667
Size: 149 2518
HpaI GTT'AAC
Cuts at: 0 1670 2667
Size: 1670 997
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 672 1999 2667
Size: 181 491 1327 668
Fragments arranged by size:
1327 668 491 181
MslI CAynn'nnrTG
Cuts at: 0 871 914 2667
Size: 871 43 1753
Fragments arranged by size:
1753 871 43
NcoI C'CATG_G
Cuts at: 0 2248 2667
Size: 2248 419
NspI r_CATG'y
Cuts at: 0 874 1784 2667
Size: 874 910 883
Fragments arranged by size:
910 883 874
PciI A'CATG_T
Cuts at: 0 870 1780 2667
Size: 870 910 887
Fragments arranged by size:
910 887 870
PflMI CCAn_nnn'nTGG
Cuts at: 0 500 645 2667
Size: 500 145 2022
Fragments arranged by size:
2022 500 145
PfoI T'CCnGG_A
Cuts at: 0 1304 2667
Size: 1304 1363
PpuMI rG'GwC_Cy
Cuts at: 0 2253 2667
Size: 2253 414
PsiI TTA'TAA
Cuts at: 0 305 1451 2667
Size: 305 1146 1216
Fragments arranged by size:
1216 1146 305
PspXI vC'TCGA_Gb
Cuts at: 0 2072 2667
Size: 2072 595
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 554 586 2667
Size: 554 32 2081
Fragments arranged by size:
2081 554 32
SanDI GG'GwC_CC
Cuts at: 0 2253 2667
Size: 2253 414
SapI GCTCTTCn'nnn_
Cuts at: 0 2555 2667
Size: 2555 112
SmlI C'TyrA_G
Cuts at: 0 361 2072 2667
Size: 361 1711 595
Fragments arranged by size:
1711 595 361
SspI AAT'ATT
Cuts at: 0 1649 1776 2541 2667
Size: 1649 127 765 126
Fragments arranged by size:
1649 765 127 126
StyI C'CwwG_G
Cuts at: 0 66 138 1262 1833 2248 2667
Size: 66 72 1124 571 415 419
Fragments arranged by size:
1124 571 419 415 72 66
TatI w'GTAC_w
Cuts at: 0 421 2452 2667
Size: 421 2031 215
Fragments arranged by size:
2031 421 215
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1156 2667
Size: 1156 1511
XhoI C'TCGA_G
Cuts at: 0 2072 2667
Size: 2072 595
Enzymes that do cut:
AarI AcuI AflII AflIII AleI AlwNI ApoI AseI
AvaI AvrII BamHI BbsI BciVI BclI BglI BmrI
BpmI Bpu10I BsaAI BsaBI BseRI BsmI BspMI BsrDI
BsrFI BstBI BstXI BstYI BtgI BtsI DraI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII HpaI MmeI MslI NcoI NspI PciI
PflMI PfoI PpuMI PsiI PspXI PsrI SanDI SapI
SmlI SspI StyI TatI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AlfI
AloI ApaI ApaLI AscI AsiSI BaeI BanI BanII
BbeI BbvCI BcgI BfrBI BglII BlpI Bme1580I BmgBI
BmtI BplI BpuEI BsaI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BsrBI BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoRV FseI FspI FspAI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PmlI PpiI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TaqII
TstI Tth111I XbaI XmaI XmnI ZraI