Restriction map for
pFN21AA1402
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII AccII AclI AcsI AflII
AflIII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BbeI BbrPI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsiEI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MseI MunI MvnI NaeI
NarI NcoI NgoMIV NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tru9I
Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AccI 1582 GTCTAC GT^[AC][GT]AC
AccIII 1019 TCCGGA T^CCGGA
AcyI 802 GGCGTC G[GA]^CG[CT]C
Alw44I 913 GTGCAC G^TGCAC
AlwNI 1279 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 913 GTGCAC G^TGCAC
AspI 1732 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 98 GGATCC G^GATCC
BbsI 196 GAAGAC GAAGAC(2/6)
BlpI 1059 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1059 GCTGAGC GC^T[ACGT]AGC
BpuAI 196 GAAGAC GAAGAC(2/6)
BsaHI 802 GGCGTC G[GA]^CG[CT]C
BseAI 1019 TCCGGA T^CCGGA
BsiWI 364 CGTACG C^GTACG
BsmI 1827 GAATGC GAATGC(1/-1)
BspEI 1019 TCCGGA T^CCGGA
BsrDI 1562 CATTGC GCAATG(2/0)
BstXI 1535 CCAATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1059 GCTGAGC GC^T[ACGT]AGC
EcoNI 221 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1559 ATGCAT ATGCA^T
Hin1I 802 GGCGTC G[GA]^CG[CT]C
MroI 1019 TCCGGA T^CCGGA
NdeI 554 CATATG CA^TATG
NheI 1192 GCTAGC G^CTAGC
NsiI 1559 ATGCAT ATGCA^T
NspI 1557 ACATGC [GA]CATG^[CT]
PaeR7I 1519 CTCGAG C^TCGAG
PflFI 1732 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SgrAI 1372 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1519 CTCGAG C^T[CT][GA]AG
Tth111I 1732 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 341 CCACTTTGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1519 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
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Acc65I 931 GGTACC G^GTACC
1664 GGTACC
Aor51HI 36 AGCGCT AGC^GCT
964 AGCGCT
Asp718I 931 GGTACC G^GTACC
1664 GGTACC
BalI 588 TGGCCA TGG^CCA
854 TGGCCA
BbvCI 594 CCTCAGC CCTCAGC(-5/-2)
2238 CCTCAGC
BsaI 484 GAGACC GGTCTC(1/5)
1737 GGTCTC
BsaWI 1019 TCCGGA [AT]^CCGG[AT]
2126 TCCGGT
BssSI 1550 CACGAG CACGAG(-5/-1)
1592 CACGAG
EarI 1765 CTCTTC CTCTTC(1/4)
2080 GAAGAG
Eco47III 36 AGCGCT AGC^GCT
964 AGCGCT
HgaI 779 GACGC GACGC(5/10)
803 GCGTC
KpnI 931 GGTACC GGTAC^C
1664 GGTACC
Ksp632I 1765 CTCTTC CTCTTC(1/4)
2080 GAAGAG
MaeII 1205 ACGT A^CGT
1733 ACGT
MluNI 588 TGGCCA TGG^CCA
854 TGGCCA
MscI 588 TGGCCA TGG^CCA
854 TGGCCA
PflMI 1621 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1778 CCACCGTGTGG
PpuMI 230 GGGACCC [GA]G^G[AT]CC[CT]
866 GGGACCC
PvuII 1468 CAGCTG CAG^CTG
2272 CAGCTG
TfiI 159 GAATC G^A[AT]TC
166 GATTC
Van91I 1621 CCATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1778 CCACCGTGTGG
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# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AspHI AvaI AvaII BanI BanII
BbvI BcgI BcnI BfaI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StuI TaqI TseI Tsp45I TspRI
TthHB8I XhoII XmaCI XmaI