(Circular) (Six-base) MAPSORT of: pFN21AA1402.seq Check: 5009 from: 1 to: 7021 pFN21AA1402 7021 bp With 182 enzymes: SgfI * February 27, 2009 14:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2434 4055 2434 Size: 1621 5400 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4831 278 Size: 53 83 186 936 3295 2468 Fragments arranged by size: 3295 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3588 4364 1937 Size: 1651 776 4594 Fragments arranged by size: 4594 1651 776 Acc65I G'GTAC_C Cuts at: 2936 3669 4342 2936 Size: 733 673 5615 Fragments arranged by size: 5615 733 673 AclI AA'CG_TT Cuts at: 1563 5148 5521 1563 Size: 3585 373 3063 Fragments arranged by size: 3585 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2950 3519 5084 6468 6736 1632 Size: 1318 569 1565 1384 268 1917 Fragments arranged by size: 1917 1565 1384 1318 569 268 AfeI AGC'GCT Cuts at: 2043 2971 2043 Size: 928 6093 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6824 AflIII A'CryG_T Cuts at: 1203 5920 6897 1203 Size: 4717 977 1327 Fragments arranged by size: 4717 1327 977 AgeI A'CCGG_T Cuts at: 5852 5852 Size: 7021 AhdI GACnn_n'nnGTC Cuts at: 5750 5750 Size: 7021 AleI CACnn'nnGTG Cuts at: 1166 2561 3467 1166 Size: 1395 906 4720 Fragments arranged by size: 4720 1395 906 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4026 1184 Size: 2842 4179 AlwNI CAG_nnn'CTG Cuts at: 3289 4764 6336 3289 Size: 1475 1572 3974 Fragments arranged by size: 3974 1572 1475 ApaI G_GGCC'C Cuts at: 2425 3388 3807 3921 2425 Size: 963 419 114 5525 Fragments arranged by size: 5525 963 419 114 ApaLI G'TGCA_C Cuts at: 2918 5078 6234 2918 Size: 2160 1156 3705 Fragments arranged by size: 3705 2160 1156 ApoI r'AATT_y Cuts at: 1493 4330 4556 4708 6817 1493 Size: 2837 226 152 2109 1697 Fragments arranged by size: 2837 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5575 160 Size: 872 4543 1606 Fragments arranged by size: 4543 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7021 AvaI C'yCGr_G Cuts at: 1870 1943 2331 2424 3465 3524 3922 3969 4050 Size: 73 388 93 1041 59 398 47 81 Cuts at: 4050 4346 1870 Size: 296 4545 Fragments arranged by size: 4545 1041 398 388 296 93 81 73 59 47 BamHI G'GATC_C Cuts at: 2103 4351 4718 2103 Size: 2248 367 4406 Fragments arranged by size: 4406 2248 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2936 3375 3669 4342 5797 Size: 325 217 627 1149 439 294 673 1455 Cuts at: 5797 618 Size: 1842 Fragments arranged by size: 1842 1455 1149 673 627 439 325 294 217 BanII G_rGCy'C Cuts at: 1391 2018 2182 2297 2425 2431 2894 3084 3175 Size: 627 164 115 128 6 463 190 91 Cuts at: 3175 3388 3619 3807 3921 3979 4057 4146 4340 Size: 213 231 188 114 58 78 89 194 Cuts at: 4340 1391 Size: 4072 Fragments arranged by size: 4072 627 463 231 213 194 190 188 164 128 115 114 91 89 78 58 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2208 928 Size: 971 309 5741 Fragments arranged by size: 5741 971 309 BbvCI CC'TCA_GC Cuts at: 2600 4244 2600 Size: 1644 5377 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2825 2859 3230 3264 3515 Size: 34 50 34 804 34 371 34 251 Cuts at: 3515 3549 5212 5246 1903 Size: 34 1663 34 3678 Fragments arranged by size: 3678 1663 804 371 251 50 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4913 6123 4913 Size: 1210 5811 BclI T'GATC_A Cuts at: 1548 1548 Size: 7021 BfrBI ATG'CAT Cuts at: 3566 3566 Size: 7021 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2476 3079 4061 Size: 107 122 71 631 605 804 603 982 Cuts at: 4061 4280 5632 136 Size: 219 1352 1525 Fragments arranged by size: 1525 1352 982 804 631 605 603 219 122 107 71 BglII A'GATC_T Cuts at: 7016 7016 Size: 7021 BlpI GC'TnA_GC Cuts at: 3065 4438 3065 Size: 1373 5648 Bme1580I G_kGCm'C Cuts at: 1790 2425 2764 2922 3185 3378 3388 3807 3921 Size: 635 339 158 263 193 10 419 114 Cuts at: 3921 4276 5082 6238 1790 Size: 355 806 1156 2573 Fragments arranged by size: 2573 1156 806 635 419 355 339 263 193 158 114 10 BmgBI CAC'GTC Cuts at: 1318 3211 3739 1318 Size: 1893 528 4600 Fragments arranged by size: 4600 1893 528 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2418 4752 5710 Size: 416 53 83 399 24 1001 2334 958 Cuts at: 5710 442 Size: 1753 Fragments arranged by size: 2334 1753 1001 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3201 1056 Size: 2145 4876 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2311 2771 2311 Size: 460 6561 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2415 2996 3257 3569 3603 4121 4130 4274 Size: 1176 581 261 312 34 518 9 144 Cuts at: 4274 5681 1239 Size: 1407 2579 Fragments arranged by size: 2579 1407 1176 581 518 312 261 144 34 9 Bpu10I CC'TnA_GC Cuts at: 2081 2264 2600 4244 2081 Size: 183 336 1644 4858 Fragments arranged by size: 4858 1644 336 183 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5146 6011 6309 5146 Size: 865 298 5858 Fragments arranged by size: 5858 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2483 3748 5684 882 Size: 617 984 1265 1936 2219 Fragments arranged by size: 2219 1936 1265 984 617 BsaAI yAC'GTr Cuts at: 493 1204 4812 493 Size: 711 3608 2702 Fragments arranged by size: 3608 2702 711 BsaBI GATnn'nnATC Cuts at: 7015 7015 Size: 7021 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2808 4828 5210 5845 Size: 53 83 186 936 1275 2020 382 635 Cuts at: 5845 275 Size: 1451 Fragments arranged by size: 2020 1451 1275 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3024 4131 5454 5852 6126 6273 6684 3024 Size: 1107 1323 398 274 147 411 3361 Fragments arranged by size: 3361 1323 1107 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2691 2721 2912 2942 3140 Size: 30 165 30 1288 30 191 30 198 Cuts at: 3140 3170 3929 3959 1178 Size: 30 759 30 4240 Fragments arranged by size: 4240 1288 759 198 191 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2166 2651 2760 4103 1186 Size: 980 485 109 1343 4104 Fragments arranged by size: 4104 1343 980 485 109 BseYI C'CCAG_C Cuts at: 2290 3121 3126 3316 4275 6224 6720 2290 Size: 831 5 190 959 1949 496 2591 Fragments arranged by size: 2591 1949 959 831 496 190 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2095 2585 3183 3491 4199 1163 Size: 573 359 490 598 308 708 3985 Fragments arranged by size: 3985 708 598 573 490 359 308 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4471 5232 6260 1540 Size: 275 186 2470 761 1028 2301 Fragments arranged by size: 2470 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2018 2182 2265 2894 2922 3619 5082 5167 6238 Size: 164 83 629 28 697 1463 85 1071 Cuts at: 6238 2018 Size: 2801 Fragments arranged by size: 2801 1463 1071 697 629 164 85 83 28 BsiWI C'GTAC_G Cuts at: 2369 2369 Size: 7021 BsmI GAATG_Cn' Cuts at: 3838 4532 4625 3838 Size: 694 93 6234 Fragments arranged by size: 6234 694 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2182 2265 2297 2425 2431 2764 Size: 399 228 164 83 32 128 6 333 Cuts at: 2764 2894 2922 3084 3175 3185 3378 3388 3619 Size: 130 28 162 91 10 193 10 231 Cuts at: 3619 3807 3921 3979 4057 4146 4276 4340 5082 Size: 188 114 58 78 89 130 64 742 Cuts at: 5082 5167 6238 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 333 231 228 193 188 164 162 130 130 128 114 91 89 85 83 78 64 58 32 28 10 10 6 BspEI T'CCGG_A Cuts at: 3024 6684 3024 Size: 3660 3361 BspHI T'CATG_A Cuts at: 4910 6640 4910 Size: 1730 5291 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2434 3354 4055 4376 844 Size: 1590 920 701 321 3489 Fragments arranged by size: 3489 1590 920 701 321 BsrBI CCG'CTC Cuts at: 1394 1823 2305 2467 2986 4908 1394 Size: 429 482 162 519 1922 3507 Fragments arranged by size: 3507 1922 519 482 429 162 BsrDI GCAATG_nn' Cuts at: 65 1248 3566 5516 5690 65 Size: 1183 2318 1950 174 1396 Fragments arranged by size: 2318 1950 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2949 3378 3999 5665 5852 1700 Size: 110 1139 429 621 1666 187 2869 Fragments arranged by size: 2869 1666 1139 621 429 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7021 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7021 BssSI C'ACGA_G Cuts at: 3555 3597 5081 6093 3555 Size: 42 1484 1012 4483 Fragments arranged by size: 4483 1484 1012 42 BstAPI GCAn_nnn'nTGC Cuts at: 794 2588 3289 3478 794 Size: 1794 701 189 4337 Fragments arranged by size: 4337 1794 701 189 BstBI TT'CG_AA Cuts at: 5825 5825 Size: 7021 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7021 BstXI CCAn_nnnn'nTGG Cuts at: 3547 3547 Size: 7021 BstYI r'GATC_y Cuts at: 1972 2103 2122 3021 4351 4718 5103 5120 6572 Size: 131 19 899 1330 367 385 17 1452 Cuts at: 6572 6658 6670 7016 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1330 899 385 367 346 131 86 19 17 12 BtgI C'CryG_G Cuts at: 513 1065 2355 3610 4208 513 Size: 552 1290 1255 598 3326 Fragments arranged by size: 3326 1290 1255 598 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6282 Fragments arranged by size: 6282 683 56 BtsI GCAGTG_nn' Cuts at: 4537 5331 5351 4537 Size: 794 20 6207 Fragments arranged by size: 6207 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7021 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6986 DraI TTT'AAA Cuts at: 4325 4325 Size: 7021 DraIII CAC_nnn'GTG Cuts at: 3371 3760 3789 3371 Size: 389 29 6603 Fragments arranged by size: 6603 389 29 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6028 809 Size: 507 4712 1802 Fragments arranged by size: 4712 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2036 2352 2593 Size: 54 1425 50 275 18 206 316 241 Cuts at: 2593 2723 2859 3413 3607 4468 5357 6986 8 Size: 130 136 554 194 861 889 1629 43 Fragments arranged by size: 1629 1425 889 861 554 316 275 241 206 194 136 130 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4468 1537 Size: 275 2656 4090 Fragments arranged by size: 4090 2656 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3776 4080 4951 1353 Size: 2423 304 871 3423 Fragments arranged by size: 3423 2423 871 304 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3221 5608 5982 6128 387 Size: 1064 138 1632 2387 374 146 1280 Fragments arranged by size: 2387 1632 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2016 2180 2892 3617 2016 Size: 164 712 725 5420 Fragments arranged by size: 5420 725 712 164 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2415 2950 2996 3257 3519 3569 3603 Size: 393 783 535 46 261 262 50 34 Cuts at: 3603 4121 4130 4274 5084 5681 6468 6736 1239 Size: 518 9 144 810 597 787 268 1524 Fragments arranged by size: 1524 810 787 783 597 535 518 393 268 262 261 144 50 46 34 9 EcoNI CCTnn'n_nnAGG Cuts at: 2230 2230 Size: 7021 EcoO109I rG'GnC_Cy Cuts at: 2236 2421 2569 2872 3385 3712 3917 3918 2236 Size: 185 148 303 513 327 205 1 5339 Fragments arranged by size: 5339 513 327 303 205 185 148 1 EcoRI G'AATT_C Cuts at: 4330 4708 4330 Size: 378 6643 FspI TGC'GCA Cuts at: 5527 5527 Size: 7021 HaeII r_GCGC'y Cuts at: 1678 2045 2973 3256 3310 6168 1678 Size: 367 928 283 54 2858 2531 Fragments arranged by size: 2858 2531 928 367 283 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2775 2807 3728 3760 3929 Size: 32 135 32 1203 32 921 32 169 Cuts at: 3929 3961 5662 5694 5736 5768 1373 Size: 32 1701 32 42 32 2626 Fragments arranged by size: 2626 1701 1203 921 169 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4365 4611 669 Size: 988 281 2427 246 3079 Fragments arranged by size: 3079 2427 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7021 HpaI GTT'AAC Cuts at: 4611 4611 Size: 7021 KpnI G_GTAC'C Cuts at: 2940 3673 4346 2940 Size: 733 673 5615 Fragments arranged by size: 5615 733 673 MfeI C'AATT_G Cuts at: 4620 4620 Size: 7021 MluI A'CGCG_T Cuts at: 6897 6897 Size: 7021 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2961 3204 3333 6135 6319 1305 Size: 1656 243 129 2802 184 2007 Fragments arranged by size: 2802 2007 1656 243 184 129 MscI TGG'CCA Cuts at: 10 64 1489 1832 2595 2861 10 Size: 54 1425 343 763 266 4170 Fragments arranged by size: 4170 1425 763 343 266 54 MslI CAynn'nnrTG Cuts at: 518 1166 2460 2561 2733 3467 3737 4979 5338 Size: 648 1294 101 172 734 270 1242 359 Cuts at: 5338 5497 518 Size: 159 2042 Fragments arranged by size: 2042 1294 1242 734 648 359 270 172 159 101 MspA1I CmG'CkG Cuts at: 1606 2416 3475 3653 3822 4236 4279 4437 5114 Size: 810 1059 178 169 414 43 158 677 Cuts at: 5114 6262 6507 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1059 810 677 414 245 178 169 158 43 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6469 NdeI CA'TA_TG Cuts at: 387 2560 387 Size: 2173 4848 NheI G'CTAG_C Cuts at: 1052 3197 1052 Size: 2145 4876 NotI GC'GGCC_GC Cuts at: 4468 4468 Size: 7021 NsiI A_TGCA'T Cuts at: 3568 3568 Size: 7021 NspI r_CATG'y Cuts at: 1132 1229 3566 4379 5924 1132 Size: 97 2337 813 1545 2229 Fragments arranged by size: 2337 2229 1545 813 97 PasI CC'CwG_GG Cuts at: 1348 3674 3914 1348 Size: 2326 240 4455 Fragments arranged by size: 4455 2326 240 PciI A'CATG_T Cuts at: 5920 5920 Size: 7021 PflMI CCAn_nnn'nTGG Cuts at: 1108 3632 3789 1108 Size: 2524 157 4340 Fragments arranged by size: 4340 2524 157 PfoI T'CCnGG_A Cuts at: 2099 3546 3873 2099 Size: 1447 327 5247 Fragments arranged by size: 5247 1447 327 PmeI GTTT'AAAC Cuts at: 4325 4325 Size: 7021 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5090 5122 6603 6635 5090 Size: 32 1481 32 5476 Fragments arranged by size: 5476 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2236 2872 2236 Size: 636 6385 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7021 PsiI TTA'TAA Cuts at: 4591 4591 Size: 7021 PspOMI G'GGCC_C Cuts at: 2421 3384 3803 3917 2421 Size: 963 419 114 5525 Fragments arranged by size: 5525 963 419 114 PspXI vC'TCGA_Gb Cuts at: 3524 3524 Size: 7021 PstI C_TGCA'G Cuts at: 830 3015 3511 3649 4205 4373 830 Size: 2185 496 138 556 168 3478 Fragments arranged by size: 3478 2185 556 496 168 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7021 PvuII CAG'CTG Cuts at: 3475 4279 3475 Size: 804 6217 SacI G_AGCT'C Cuts at: 2018 2182 2894 3619 2018 Size: 164 712 725 5420 Fragments arranged by size: 5420 725 712 164 SalI G'TCGA_C Cuts at: 1936 4363 1936 Size: 2427 4594 SanDI GG'GwC_CC Cuts at: 2236 2872 2236 Size: 636 6385 SbfI CC_TGCA'GG Cuts at: 4373 4373 Size: 7021 ScaI AGT'ACT Cuts at: 5269 6981 5269 Size: 1712 5309 SfcI C'TryA_G Cuts at: 826 1045 2139 2230 3011 3188 3507 3645 3981 Size: 219 1094 91 781 177 319 138 336 Cuts at: 3981 4201 4369 5504 6185 6376 826 Size: 220 168 1135 681 191 1471 Fragments arranged by size: 1471 1135 1094 781 681 336 319 220 219 191 177 168 138 91 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7021 SgrAI Cr'CCGG_yG Cuts at: 1700 3378 1700 Size: 1678 5343 SmaI CCC'GGG Cuts at: 1872 2426 3924 3971 4348 1872 Size: 554 1498 47 377 4545 Fragments arranged by size: 4545 1498 554 377 47 SmlI C'TyrA_G Cuts at: 820 1017 1943 3524 5125 6026 6288 820 Size: 197 926 1581 1601 901 262 1553 Fragments arranged by size: 1601 1581 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4812 493 Size: 4319 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7021 SphI G_CATG'C Cuts at: 1132 4379 1132 Size: 3247 3774 SrfI GCCC'GGGC Cuts at: 2426 2426 Size: 7021 SspI AAT'ATT Cuts at: 5 52 4945 5 Size: 47 4893 2081 Fragments arranged by size: 4893 2081 47 StuI AGG'CCT Cuts at: 1517 3530 3799 3854 1517 Size: 2013 269 55 4684 Fragments arranged by size: 4684 2013 269 55 StyI C'CwwG_G Cuts at: 513 1065 4460 513 Size: 552 3395 3074 Fragments arranged by size: 3395 3074 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3203 5048 5065 5218 5403 5848 325 Size: 1255 1623 1845 17 153 185 445 1498 Fragments arranged by size: 1845 1623 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2619 3333 5267 Size: 274 80 33 51 1443 642 714 1934 Cuts at: 5267 6979 96 Size: 1712 138 Fragments arranged by size: 1934 1712 1443 714 642 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2691 2723 2912 2944 3140 3172 6981 7013 2691 Size: 32 189 32 196 32 3809 32 2699 Fragments arranged by size: 3809 2699 196 189 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3740 1316 Size: 2424 4597 XbaI T'CTAG_A Cuts at: 4357 4357 Size: 7021 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2353 2353 Size: 7021 XhoI C'TCGA_G Cuts at: 1943 3524 1943 Size: 1581 5440 XmaI C'CCGG_G Cuts at: 1870 2424 3922 3969 4346 1870 Size: 554 1498 47 377 4545 Fragments arranged by size: 4545 1498 554 377 47 XmnI GAAnn'nnTTC Cuts at: 5150 5150 Size: 7021 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4829 276 Size: 53 83 186 936 3295 2468 Fragments arranged by size: 3295 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI RsrII SacII SapI SexAI SfiI SfoI SwaI