(Circular) (Six-base) MAPSORT of: pFN21AA1390.seq Check: 732 from: 1 to: 6112 pFN21AA1390 6112 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2813 2813 Size: 6112 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3922 278 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2669 3171 3455 1937 Size: 732 502 284 4594 Fragments arranged by size: 4594 732 502 284 Acc65I G'GTAC_C Cuts at: 3433 3433 Size: 6112 AclI AA'CG_TT Cuts at: 1563 4239 4612 1563 Size: 2676 373 3063 Fragments arranged by size: 3063 2676 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2238 2283 2432 2546 3245 4175 5559 5827 Size: 606 45 149 114 699 930 1384 268 Cuts at: 5827 1632 Size: 1917 Fragments arranged by size: 1917 1384 930 699 606 268 149 114 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5915 AflIII A'CryG_T Cuts at: 1203 5011 5988 1203 Size: 3808 977 1327 Fragments arranged by size: 3808 1327 977 AgeI A'CCGG_T Cuts at: 4943 4943 Size: 6112 AhdI GACnn_n'nnGTC Cuts at: 4841 4841 Size: 6112 AleI CACnn'nnGTG Cuts at: 1166 2677 2817 1166 Size: 1511 140 4461 Fragments arranged by size: 4461 1511 140 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6112 AlwNI CAG_nnn'CTG Cuts at: 2036 2863 2918 3855 5427 2036 Size: 827 55 937 1572 2721 Fragments arranged by size: 2721 1572 937 827 55 ApaLI G'TGCA_C Cuts at: 4169 5325 4169 Size: 1156 4956 ApoI r'AATT_y Cuts at: 1493 3421 3647 3799 5908 1493 Size: 1928 226 152 2109 1697 Fragments arranged by size: 2109 1928 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4666 160 Size: 872 3634 1606 Fragments arranged by size: 3634 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 AvaI C'yCGr_G Cuts at: 1870 1943 2675 3437 1870 Size: 73 732 762 4545 Fragments arranged by size: 4545 762 732 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2696 2729 2696 Size: 33 6079 BamHI G'GATC_C Cuts at: 2026 2358 3442 3809 2026 Size: 332 1084 367 4329 Fragments arranged by size: 4329 1084 367 332 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3433 4888 618 Size: 325 217 627 1646 1455 1842 Fragments arranged by size: 1842 1646 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2502 3431 1391 Size: 1111 929 4072 Fragments arranged by size: 4072 1111 929 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4303 4337 1903 Size: 34 2366 34 3678 Fragments arranged by size: 3678 2366 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4004 5214 4004 Size: 1210 4902 BclI T'GATC_A Cuts at: 1548 2484 1548 Size: 936 5176 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4723 136 Size: 107 122 71 631 605 3051 1525 Fragments arranged by size: 3051 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6107 6107 Size: 6112 BlpI GC'TnA_GC Cuts at: 2227 3529 2227 Size: 1302 4810 Bme1580I G_kGCm'C Cuts at: 1790 4173 5329 1790 Size: 2383 1156 2573 Fragments arranged by size: 2573 2383 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6112 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3843 4801 442 Size: 416 53 83 399 24 2426 958 1753 Fragments arranged by size: 2426 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2226 1056 Size: 1170 4942 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2800 2885 3147 4772 1239 Size: 1561 85 262 1625 2579 Fragments arranged by size: 2579 1625 1561 262 85 Bpu10I CC'TnA_GC Cuts at: 2331 2922 2331 Size: 591 5521 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2222 4237 5102 5400 2222 Size: 2015 865 298 2934 Fragments arranged by size: 2934 2015 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2861 4775 882 Size: 617 1362 1914 2219 Fragments arranged by size: 2219 1914 1362 617 BsaAI yAC'GTr Cuts at: 493 1204 3903 493 Size: 711 2699 2702 Fragments arranged by size: 2702 2699 711 BsaBI GATnn'nnATC Cuts at: 6106 6106 Size: 6112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2652 3355 3919 4301 Size: 53 83 186 936 1119 703 564 382 Cuts at: 4301 4936 275 Size: 635 1451 Fragments arranged by size: 1451 1119 936 703 635 564 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4545 4943 5217 5364 5775 4545 Size: 398 274 147 411 4882 Fragments arranged by size: 4882 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5887 Fragments arranged by size: 5887 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3044 3284 3354 1186 Size: 1858 240 70 3944 Fragments arranged by size: 3944 1858 240 70 BseYI C'CCAG_C Cuts at: 2880 3226 3252 3358 5315 5811 2880 Size: 346 26 106 1957 496 3181 Fragments arranged by size: 3181 1957 496 346 106 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2701 2778 3038 1163 Size: 573 965 77 260 4237 Fragments arranged by size: 4237 965 573 260 77 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3562 4323 5351 1540 Size: 275 186 1561 761 1028 2301 Fragments arranged by size: 2301 1561 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 2402 2502 4173 4258 5329 2106 Size: 296 100 1671 85 1071 2889 Fragments arranged by size: 2889 1671 1071 296 100 85 BsmI GAATG_Cn' Cuts at: 3402 3623 3716 3402 Size: 221 93 5798 Fragments arranged by size: 5798 221 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2659 2659 Size: 6112 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2402 2502 3431 4173 4258 5329 Size: 399 316 296 100 929 742 85 1071 Cuts at: 5329 1391 Size: 2174 Fragments arranged by size: 2174 1071 929 742 399 316 296 100 85 BspEI T'CCGG_A Cuts at: 5775 5775 Size: 6112 BspHI T'CATG_A Cuts at: 2430 4001 5731 2430 Size: 1571 1730 2811 Fragments arranged by size: 2811 1730 1571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2703 2813 3157 3467 844 Size: 1859 110 344 310 3489 Fragments arranged by size: 3489 1859 344 310 110 BsrBI CCG'CTC Cuts at: 1394 1823 3999 1394 Size: 429 2176 3507 Fragments arranged by size: 3507 2176 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2601 4607 4781 65 Size: 1183 1353 2006 174 1396 Fragments arranged by size: 2006 1396 1353 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4756 4943 1700 Size: 110 2946 187 2869 Fragments arranged by size: 2946 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6112 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6112 BssSI C'ACGA_G Cuts at: 2831 3216 3389 4172 5184 2831 Size: 385 173 783 1012 3759 Fragments arranged by size: 3759 1012 783 385 173 BstAPI GCAn_nnn'nTGC Cuts at: 794 2129 3301 794 Size: 1335 1172 3605 Fragments arranged by size: 3605 1335 1172 BstBI TT'CG_AA Cuts at: 4916 4916 Size: 6112 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6112 BstXI CCAn_nnnn'nTGG Cuts at: 2626 2626 Size: 6112 BstYI r'GATC_y Cuts at: 1972 2026 2358 3442 3809 4194 4211 5663 5749 Size: 54 332 1084 367 385 17 1452 86 Cuts at: 5749 5761 6107 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1084 385 367 346 332 86 54 17 12 BstZ17I GTA'TAC Cuts at: 2670 2670 Size: 6112 Bsu36I CC'TnA_GG Cuts at: 2022 2109 2745 2022 Size: 87 636 5389 Fragments arranged by size: 5389 636 87 BtgI C'CryG_G Cuts at: 513 1065 2004 2716 2874 2893 513 Size: 552 939 712 158 19 3732 Fragments arranged by size: 3732 939 712 552 158 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5373 Fragments arranged by size: 5373 683 56 BtsI GCAGTG_nn' Cuts at: 2255 2689 3326 3628 4422 4442 2255 Size: 434 637 302 794 20 3925 Fragments arranged by size: 3925 794 637 434 302 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6077 DraI TTT'AAA Cuts at: 3416 3416 Size: 6112 DraIII CAC_nnn'GTG Cuts at: 3061 3061 Size: 6112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5119 809 Size: 507 3803 1802 Fragments arranged by size: 3803 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2616 3559 4448 Size: 54 1425 50 275 18 786 943 889 Cuts at: 4448 6077 8 Size: 1629 43 Fragments arranged by size: 1629 1425 943 889 786 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3559 1537 Size: 275 1747 4090 Fragments arranged by size: 4090 1747 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4042 1353 Size: 2689 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4699 5073 5219 387 Size: 1064 138 3110 374 146 1280 Fragments arranged by size: 3110 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2500 2500 Size: 6112 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2238 2283 2432 2546 2800 2885 3147 Size: 393 606 45 149 114 254 85 262 Cuts at: 3147 3245 4175 4772 5559 5827 1239 Size: 98 930 597 787 268 1524 Fragments arranged by size: 1524 930 787 606 597 393 268 262 254 149 114 98 85 45 EcoNI CCTnn'n_nnAGG Cuts at: 2367 3116 2367 Size: 749 5363 EcoO109I rG'GnC_Cy Cuts at: 2319 3122 3319 2319 Size: 803 197 5112 Fragments arranged by size: 5112 803 197 EcoRI G'AATT_C Cuts at: 3421 3799 3421 Size: 378 5734 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3007 3007 Size: 6112 FspI TGC'GCA Cuts at: 4618 4618 Size: 6112 HaeII r_GCGC'y Cuts at: 1678 5259 1678 Size: 3581 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4753 4785 4827 4859 1373 Size: 32 135 32 3181 32 42 32 2626 Fragments arranged by size: 3181 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3456 3702 669 Size: 988 281 1518 246 3079 Fragments arranged by size: 3079 1518 988 281 246 HindIII A'AGCT_T Cuts at: 748 2169 748 Size: 1421 4691 HpaI GTT'AAC Cuts at: 3702 3702 Size: 6112 KpnI G_GTAC'C Cuts at: 3437 3437 Size: 6112 MfeI C'AATT_G Cuts at: 3711 3711 Size: 6112 MluI A'CGCG_T Cuts at: 5988 5988 Size: 6112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5226 5410 1305 Size: 3921 184 2007 Fragments arranged by size: 3921 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2618 10 Size: 54 1425 343 786 3504 Fragments arranged by size: 3504 1425 786 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2677 2817 4070 4429 4588 518 Size: 648 1511 140 1253 359 159 2042 Fragments arranged by size: 2042 1511 1253 648 359 159 140 MspA1I CmG'CkG Cuts at: 1606 2785 2876 2884 2918 3075 3163 3226 3528 Size: 1179 91 8 34 157 88 63 302 Cuts at: 3528 4205 5353 5598 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1179 1148 677 302 245 157 91 88 63 34 8 NcoI C'CATG_G Cuts at: 513 1065 2004 2716 513 Size: 552 939 712 3909 Fragments arranged by size: 3909 939 712 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6112 NheI G'CTAG_C Cuts at: 1052 2222 1052 Size: 1170 4942 NotI GC'GGCC_GC Cuts at: 3559 3559 Size: 6112 NspI r_CATG'y Cuts at: 1132 1229 2286 3470 5015 1132 Size: 97 1057 1184 1545 2229 Fragments arranged by size: 2229 1545 1184 1057 97 PasI CC'CwG_GG Cuts at: 1348 3112 1348 Size: 1764 4348 PciI A'CATG_T Cuts at: 5011 5011 Size: 6112 PflMI CCAn_nnn'nTGG Cuts at: 1108 2036 2249 2788 1108 Size: 928 213 539 4432 Fragments arranged by size: 4432 928 539 213 PfoI T'CCnGG_A Cuts at: 2862 2862 Size: 6112 PmeI GTTT'AAAC Cuts at: 3416 3416 Size: 6112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4181 4213 5694 5726 4181 Size: 32 1481 32 4567 Fragments arranged by size: 4567 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2319 3319 2319 Size: 1000 5112 PshAI GACnn'nnGTC Cuts at: 1613 3317 1613 Size: 1704 4408 PsiI TTA'TAA Cuts at: 3682 3682 Size: 6112 PstI C_TGCA'G Cuts at: 830 2056 3168 3204 3333 3464 830 Size: 1226 1112 36 129 131 3478 Fragments arranged by size: 3478 1226 1112 131 129 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6112 PvuII CAG'CTG Cuts at: 2785 2884 2918 3075 3163 2785 Size: 99 34 157 88 5734 Fragments arranged by size: 5734 157 99 88 34 SacI G_AGCT'C Cuts at: 2502 2502 Size: 6112 SacII CC_GC'GG Cuts at: 2877 2877 Size: 6112 SalI G'TCGA_C Cuts at: 1936 3454 1936 Size: 1518 4594 SbfI CC_TGCA'GG Cuts at: 3464 3464 Size: 6112 ScaI AGT'ACT Cuts at: 4360 6072 4360 Size: 1712 4400 SfcI C'TryA_G Cuts at: 826 1045 2052 3023 3164 3200 3329 3460 4595 Size: 219 1007 971 141 36 129 131 1135 Cuts at: 4595 5276 5467 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1007 971 681 219 191 141 131 129 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6112 SmaI CCC'GGG Cuts at: 1872 3439 1872 Size: 1567 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2201 4216 5117 5379 820 Size: 197 926 258 2015 901 262 1553 Fragments arranged by size: 2015 1553 926 901 262 258 197 SnaBI TAC'GTA Cuts at: 493 3903 493 Size: 3410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6112 SphI G_CATG'C Cuts at: 1132 2286 3470 1132 Size: 1154 1184 3774 Fragments arranged by size: 3774 1184 1154 SspI AAT'ATT Cuts at: 5 52 4036 5 Size: 47 3984 2081 Fragments arranged by size: 3984 2081 47 StuI AGG'CCT Cuts at: 1517 2844 1517 Size: 1327 4785 StyI C'CwwG_G Cuts at: 513 1065 2004 2248 2363 2716 3551 513 Size: 552 939 244 115 353 835 3074 Fragments arranged by size: 3074 939 835 552 353 244 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2398 2408 4139 4156 4309 4494 4939 Size: 1255 818 10 1731 17 153 185 445 Cuts at: 4939 325 Size: 1498 Fragments arranged by size: 1731 1498 1255 818 445 185 153 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4358 6070 96 Size: 274 80 33 51 1443 2381 1712 138 Fragments arranged by size: 2381 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6072 6104 6072 Size: 32 6080 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6112 XbaI T'CTAG_A Cuts at: 3448 3448 Size: 6112 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6112 XmaI C'CCGG_G Cuts at: 1870 3437 1870 Size: 1567 4545 XmnI GAAnn'nnTTC Cuts at: 4241 4241 Size: 6112 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3920 276 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI