(Circular) (Six-base) MAPSORT of: pFN21AA1385.seq Check: 4117 from: 1 to: 6913 pFN21AA1385 6913 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3745 3914 3745 Size: 169 6744 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4723 278 Size: 53 83 186 936 3187 2468 Fragments arranged by size: 3187 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4033 4256 1937 Size: 2096 223 4594 Fragments arranged by size: 4594 2096 223 Acc65I G'GTAC_C Cuts at: 4234 4234 Size: 6913 AclI AA'CG_TT Cuts at: 1563 5040 5413 1563 Size: 3477 373 3063 Fragments arranged by size: 3477 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2068 2790 3345 3564 4976 6360 6628 1632 Size: 436 722 555 219 1412 1384 268 1917 Fragments arranged by size: 1917 1412 1384 722 555 436 268 219 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6716 AflIII A'CryG_T Cuts at: 1203 5812 6789 1203 Size: 4609 977 1327 Fragments arranged by size: 4609 1327 977 AgeI A'CCGG_T Cuts at: 5744 5744 Size: 6913 AhdI GACnn_n'nnGTC Cuts at: 5642 5642 Size: 6913 AleI CACnn'nnGTG Cuts at: 1166 2936 1166 Size: 1770 5143 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6913 AlwNI CAG_nnn'CTG Cuts at: 3760 4656 6228 3760 Size: 896 1572 4445 Fragments arranged by size: 4445 1572 896 ApaI G_GGCC'C Cuts at: 3440 3440 Size: 6913 ApaLI G'TGCA_C Cuts at: 4970 6126 4970 Size: 1156 5757 ApoI r'AATT_y Cuts at: 1493 3342 4222 4448 4600 6709 1493 Size: 1849 880 226 152 2109 1697 Fragments arranged by size: 2109 1849 1697 880 226 152 AseI AT'TA_AT Cuts at: 160 1032 5467 160 Size: 872 4435 1606 Fragments arranged by size: 4435 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6913 AvaI C'yCGr_G Cuts at: 1870 1943 4238 1870 Size: 73 2295 4545 Fragments arranged by size: 4545 2295 73 BamHI G'GATC_C Cuts at: 3955 4243 4610 3955 Size: 288 367 6258 Fragments arranged by size: 6258 367 288 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4234 5689 618 Size: 325 217 627 2447 1455 1842 Fragments arranged by size: 2447 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3440 4170 4232 1391 Size: 2049 730 62 4072 Fragments arranged by size: 4072 2049 730 62 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2106 2628 2953 3446 928 Size: 971 207 522 325 493 4395 Fragments arranged by size: 4395 971 522 493 325 207 BbvCI CC'TCA_GC Cuts at: 2792 2792 Size: 6913 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5104 5138 1903 Size: 34 3167 34 3678 Fragments arranged by size: 3678 3167 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3736 4805 6015 3736 Size: 1069 1210 4634 Fragments arranged by size: 4634 1210 1069 BclI T'GATC_A Cuts at: 1548 1548 Size: 6913 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5524 136 Size: 107 122 71 631 605 3852 1525 Fragments arranged by size: 3852 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2125 6908 2125 Size: 4783 2130 BlpI GC'TnA_GC Cuts at: 4330 4330 Size: 6913 Bme1580I G_kGCm'C Cuts at: 1790 3440 4072 4974 6130 1790 Size: 1650 632 902 1156 2573 Fragments arranged by size: 2573 1650 1156 902 632 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6913 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4644 5602 442 Size: 416 53 83 399 24 3227 958 1753 Fragments arranged by size: 3227 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2277 2280 3026 5573 1239 Size: 1038 3 746 2547 2579 Fragments arranged by size: 2579 2547 1038 746 3 Bpu10I CC'TnA_GC Cuts at: 2792 2823 2792 Size: 31 6882 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3057 3734 5038 5903 6201 3057 Size: 677 1304 865 298 3769 Fragments arranged by size: 3769 1304 865 677 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5576 882 Size: 617 4077 2219 Fragments arranged by size: 4077 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2513 4162 4704 493 Size: 711 1309 1649 542 2702 Fragments arranged by size: 2702 1649 1309 711 542 BsaBI GATnn'nnATC Cuts at: 6907 6907 Size: 6913 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4720 5102 5737 275 Size: 53 83 186 936 3187 382 635 1451 Fragments arranged by size: 3187 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3016 5346 5744 6018 6165 6576 3016 Size: 2330 398 274 147 411 3353 Fragments arranged by size: 3353 2330 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2771 2801 3232 3262 1178 Size: 30 165 30 1368 30 431 30 4829 Fragments arranged by size: 4829 1368 431 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2580 1186 Size: 1394 5519 BseYI C'CCAG_C Cuts at: 3109 6116 6612 3109 Size: 3007 496 3410 Fragments arranged by size: 3410 3007 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2902 3912 1163 Size: 573 1166 1010 4164 Fragments arranged by size: 4164 1166 1010 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 4363 5124 6152 1540 Size: 275 186 12 2350 761 1028 2301 Fragments arranged by size: 2350 2301 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2772 2824 3250 4170 4974 5059 6130 2772 Size: 52 426 920 804 85 1071 3555 Fragments arranged by size: 3555 1071 920 804 426 85 52 BsmI GAATG_Cn' Cuts at: 2468 4424 4517 2468 Size: 1956 93 4864 Fragments arranged by size: 4864 1956 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2772 2824 3250 3440 4072 4170 4232 Size: 399 982 52 426 190 632 98 62 Cuts at: 4232 4974 5059 6130 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 982 742 632 426 399 190 98 85 62 52 BspEI T'CCGG_A Cuts at: 6576 6576 Size: 6913 BspHI T'CATG_A Cuts at: 4802 6532 4802 Size: 1730 5183 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2442 3745 3914 4268 844 Size: 1598 1303 169 354 3489 Fragments arranged by size: 3489 1598 1303 354 169 BsrBI CCG'CTC Cuts at: 1394 1823 4800 1394 Size: 429 2977 3507 Fragments arranged by size: 3507 2977 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2352 2721 5408 5582 65 Size: 1183 1104 369 2687 174 1396 Fragments arranged by size: 2687 1396 1183 1104 369 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5557 5744 1700 Size: 110 3747 187 2869 Fragments arranged by size: 3747 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6913 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6913 BssSI C'ACGA_G Cuts at: 2280 2754 2775 2817 4973 5985 2280 Size: 474 21 42 2156 1012 3208 Fragments arranged by size: 3208 2156 1012 474 42 21 BstAPI GCAn_nnn'nTGC Cuts at: 794 2723 794 Size: 1929 4984 BstBI TT'CG_AA Cuts at: 5717 5717 Size: 6913 BstEII G'GTnAC_C Cuts at: 1184 3617 3901 1184 Size: 2433 284 4196 Fragments arranged by size: 4196 2433 284 BstXI CCAn_nnnn'nTGG Cuts at: 4065 4065 Size: 6913 BstYI r'GATC_y Cuts at: 1972 2125 2137 2453 3783 3955 4243 4610 4995 Size: 153 12 316 1330 172 288 367 385 Cuts at: 4995 5012 6464 6550 6562 6908 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1330 385 367 346 316 288 172 153 86 17 12 12 BstZ17I GTA'TAC Cuts at: 4034 4034 Size: 6913 BtgI C'CryG_G Cuts at: 513 1065 2004 3729 513 Size: 552 939 1725 3697 Fragments arranged by size: 3697 1725 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2936 487 Size: 683 56 1710 4464 Fragments arranged by size: 4464 1710 683 56 BtsI GCAGTG_nn' Cuts at: 2113 4123 4429 5223 5243 2113 Size: 2010 306 794 20 3783 Fragments arranged by size: 3783 2010 794 306 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6913 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6878 DraI TTT'AAA Cuts at: 4217 4217 Size: 6913 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5920 809 Size: 507 4604 1802 Fragments arranged by size: 4604 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3359 3385 3417 Size: 54 1425 50 275 18 1529 26 32 Cuts at: 3417 3962 4360 5249 6878 8 Size: 545 398 889 1629 43 Fragments arranged by size: 1629 1529 1425 889 545 398 275 54 50 43 32 26 18 EagI C'GGCC_G Cuts at: 1537 1812 4360 1537 Size: 275 2548 4090 Fragments arranged by size: 4090 2548 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2640 2649 3597 4843 1353 Size: 1287 9 948 1246 3423 Fragments arranged by size: 3423 1287 1246 948 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5500 5874 6020 387 Size: 1064 138 3911 374 146 1280 Fragments arranged by size: 3911 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4168 4168 Size: 6913 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2277 2280 2790 3026 3345 3564 Size: 393 436 209 3 510 236 319 219 Cuts at: 3564 4976 5573 6360 6628 1239 Size: 1412 597 787 268 1524 Fragments arranged by size: 1524 1412 787 597 510 436 393 319 268 236 219 209 3 EcoO109I rG'GnC_Cy Cuts at: 3437 3437 Size: 6913 EcoRI G'AATT_C Cuts at: 3342 4222 4600 3342 Size: 880 378 5655 Fragments arranged by size: 5655 880 378 EcoRV GAT'ATC Cuts at: 2929 3373 2929 Size: 444 6469 FspI TGC'GCA Cuts at: 4115 5419 4115 Size: 1304 5609 HaeII r_GCGC'y Cuts at: 1678 6060 1678 Size: 4382 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2681 2713 2771 2803 4084 Size: 32 135 32 1109 32 58 32 1281 Cuts at: 4084 4116 4126 4158 5554 5586 5628 5660 1373 Size: 32 10 32 1396 32 42 32 2626 Fragments arranged by size: 2626 1396 1281 1109 135 58 42 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 3519 4257 4503 669 Size: 988 281 1581 738 246 3079 Fragments arranged by size: 3079 1581 988 738 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6913 HpaI GTT'AAC Cuts at: 4503 4503 Size: 6913 KpnI G_GTAC'C Cuts at: 4238 4238 Size: 6913 MfeI C'AATT_G Cuts at: 4512 4512 Size: 6913 MluI A'CGCG_T Cuts at: 6789 6789 Size: 6913 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2035 6027 6211 1305 Size: 730 3992 184 2007 Fragments arranged by size: 3992 2007 730 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3419 10 Size: 54 1425 343 1587 3504 Fragments arranged by size: 3504 1587 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2936 4871 5230 5389 518 Size: 648 1770 1935 359 159 2042 Fragments arranged by size: 2042 1935 1770 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4329 5006 6154 6399 1606 Size: 2723 677 1148 245 2120 Fragments arranged by size: 2723 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3729 513 Size: 552 939 1725 3697 Fragments arranged by size: 3697 1725 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6913 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6913 NotI GC'GGCC_GC Cuts at: 4360 4360 Size: 6913 NspI r_CATG'y Cuts at: 1132 1229 2386 2959 4114 4271 5816 1132 Size: 97 1157 573 1155 157 1545 2229 Fragments arranged by size: 2229 1545 1157 1155 573 157 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6913 PciI A'CATG_T Cuts at: 5812 5812 Size: 6913 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6913 PfoI T'CCnGG_A Cuts at: 3003 3003 Size: 6913 PmeI GTTT'AAAC Cuts at: 4217 4217 Size: 6913 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4982 5014 6495 6527 4982 Size: 32 1481 32 5368 Fragments arranged by size: 5368 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6913 PsiI TTA'TAA Cuts at: 4483 4483 Size: 6913 PspOMI G'GGCC_C Cuts at: 3436 3436 Size: 6913 PstI C_TGCA'G Cuts at: 830 2716 4265 830 Size: 1886 1549 3478 Fragments arranged by size: 3478 1886 1549 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6913 SacI G_AGCT'C Cuts at: 4170 4170 Size: 6913 SalI G'TCGA_C Cuts at: 1936 4255 1936 Size: 2319 4594 SbfI CC_TGCA'GG Cuts at: 4265 4265 Size: 6913 ScaI AGT'ACT Cuts at: 5161 6873 5161 Size: 1712 5201 SfcI C'TryA_G Cuts at: 826 1045 2712 2844 3465 4261 5396 6077 6268 Size: 219 1667 132 621 796 1135 681 191 Cuts at: 6268 826 Size: 1471 Fragments arranged by size: 1667 1471 1135 796 681 621 219 191 132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6913 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6913 SmaI CCC'GGG Cuts at: 1872 4240 1872 Size: 2368 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3072 3749 5017 5918 6180 820 Size: 197 926 1129 677 1268 901 262 1553 Fragments arranged by size: 1553 1268 1129 926 901 677 262 197 SnaBI TAC'GTA Cuts at: 493 4704 493 Size: 4211 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6913 SphI G_CATG'C Cuts at: 1132 2386 4114 4271 1132 Size: 1254 1728 157 3774 Fragments arranged by size: 3774 1728 1254 157 SspI AAT'ATT Cuts at: 5 52 4837 5 Size: 47 4785 2081 Fragments arranged by size: 4785 2081 47 StuI AGG'CCT Cuts at: 1517 2395 1517 Size: 878 6035 StyI C'CwwG_G Cuts at: 513 1065 2004 2934 3729 4064 4352 513 Size: 552 939 930 795 335 288 3074 Fragments arranged by size: 3074 939 930 795 552 335 288 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2027 2141 4940 4957 5110 5295 5740 Size: 1255 447 114 2799 17 153 185 445 Cuts at: 5740 325 Size: 1498 Fragments arranged by size: 2799 1498 1255 447 445 185 153 114 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3277 4076 5159 Size: 274 80 33 51 1443 1300 799 1083 Cuts at: 5159 6871 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1300 1083 799 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2853 2885 6873 6905 2853 Size: 32 3988 32 2861 Fragments arranged by size: 3988 2861 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6913 XbaI T'CTAG_A Cuts at: 4249 4249 Size: 6913 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3631 3631 Size: 6913 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6913 XmaI C'CCGG_G Cuts at: 1870 4238 1870 Size: 2368 4545 XmnI GAAnn'nnTTC Cuts at: 2466 5042 2466 Size: 2576 4337 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4721 276 Size: 53 83 186 936 3187 2468 Fragments arranged by size: 3187 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI