Restriction map for
pFN21AA1384
##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp718I
AspEI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaBI BseAI BsiWI BsmBI BspDI
BspEI BspLU11I BssSI Bst1107I BstBI
BstZ17I Bsu36I CelII ClaI DdeI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoRV
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I SacI
SacII SalI SbfI SfoI SfuI
SgrAI SpeI Sse8387I SspI StuI
SwaI XbaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 886 GACGCC G[GA]^CG[CT]C
Alw44I 1402 GTGCAC G^TGCAC
ApaI 236 GGGCCC GGGCC^C
ApaLI 1402 GTGCAC G^TGCAC
Asp700I 1584 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 1047 GAAGAC GAAGAC(2/6)
BfaI 1336 CTAG C^TAG
BglII 539 AGATCT A^GATCT
BpuAI 1047 GAAGAC GAAGAC(2/6)
BsaAI 1279 TACGTA [CT]AC^GT[GA]
BsaHI 886 GACGCC G[GA]^CG[CT]C
BsaWI 999 ACCGGA [AT]^CCGG[AT]
BsmI 1618 GAATGC GAATGC(1/-1)
Bsp1407I 1434 TGTACA T^GTACA
BspHI 779 TCATGA T^CATGA
BsrBI 826 GAGCGG CCGCTC(-3/-3)
BsrDI 133 CATTGC GCAATG(2/0)
BsrFI 1004 ACCGGC [GA]^CCGG[CT]
BsrGI 1434 TGTACA T^GTACA
BssHII 663 GCGCGC G^CGCGC
BstAPI 1831 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1004 ACCGGC [GA]^CCGG[CT]
CpoI 1001 CGGACCG CG^G[AT]CCG
EcoNI 624 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 947 GAATTC G^AATTC
EcoT22I 1253 ATGCAT ATGCA^T
HgaI 886 GACGC GACGC(5/10)
Hin1I 886 GACGCC G[GA]^CG[CT]C
MaeI 1336 CTAG C^TAG
MfeI 1565 CAATTG C^AATTG
MseI 1202 TTAA T^TAA
MunI 1565 CAATTG C^AATTG
NcoI 1456 CCATGG C^CATGG
NsiI 1253 ATGCAT ATGCA^T
PaeR7I 415 CTCGAG C^TCGAG
PflMI 834 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 19 AGGACCT [GA]G^G[AT]CC[CT]
PvuI 175 CGATCG CGAT^CG
RcaI 779 TCATGA T^CATGA
RsrII 1001 CGGACCG CG^G[AT]CCG
ScaI 167 AGTACT AGT^ACT
SmlI 415 CTCGAG C^T[CT][GA]AG
SnaBI 1279 TACGTA TAC^GTA
SphI 758 GCATGC GCATG^C
SspBI 1434 TGTACA T^GTACA
Tru9I 1202 TTAA T^TAA
Van91I 834 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 415 CTCGAG C^TCGAG
XmnI 1584 GAAAGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 947 GAATTC [GA]^AATT[CT]
1173 AAATTT
ApoI 947 GAATTC [GA]^AATT[CT]
1173 AAATTT
AspI 441 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
859 GACCCGGTC
BalI 611 TGGCCA TGG^CCA
938 TGGCCA
BsaI 598 GAGACC GGTCTC(1/5)
751 GAGACC
BsiEI 175 CGATCG CG[GA][CT]^CG
744 CGACCG
BsmAI 598 GAGAC GTCTC(1/5)
751 GAGAC
BstEII 504 GGTCACC G^GT[ACGT]ACC
1636 GGTGACC
BstPI 504 GGTCACC G^GT[ACGT]ACC
1636 GGTGACC
BstXI 602 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1013 CCAGCAATTTGG
DsaI 692 CCGTGG C^C[GA][CT]GG
1456 CCATGG
EcoO65I 504 GGTCACC G^GT[ACGT]ACC
1636 GGTGACC
EcoT14I 242 CCAAGG C^C[AT][AT]GG
1456 CCATGG
HphI 1636 GGTGA GGTGA(8/7)
506 TCACC
MluNI 611 TGGCCA TGG^CCA
938 TGGCCA
MscI 611 TGGCCA TGG^CCA
938 TGGCCA
MslI 1065 CACAATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1597 CACCTTGATG
MspA1I 88 CAGCTG C[AC]G^C[GT]G
1734 CAGCTG
NspI 758 GCATGC [GA]CATG^[CT]
1255 GCATGT
PflFI 441 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
859 GACCCGGTC
PstI 379 CTGCAG CTGCA^G
924 CTGCAG
PvuII 88 CAGCTG CAG^CTG
1734 CAGCTG
SexAI 371 ACCTGGT A^CC[AT]GGT
623 ACCTGGT
SfaNI 660 GATGC GCATC(5/9)
906 GATGC
SfiI 144 GGCCGTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
220 GGCCAGGACGGCC
SmaI 310 CCCGGG CCC^GGG
356 CCCGGG
StyI 242 CCAAGG C^C[AT][AT]GG
1456 CCATGG
TfiI 1149 GAATC G^A[AT]TC
1212 GATTC
Tth111I 441 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
859 GACCCGGTC
XcmI 362 CCATCAACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
614 CCAACAAGTACCTGG
XmaCI 310 CCCGGG C^CCGGG
356 CCCGGG
XmaI 310 CCCGGG C^CCGGG
356 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DpnII
DraII EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI SapI Sau3AI
Sau96I ScrFI SfcI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII